Amino acid dipepetide frequency for Bifidobacterium cuniculi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.372AlaAla: 14.372 ± 0.204
1.403AlaCys: 1.403 ± 0.045
8.112AlaAsp: 8.112 ± 0.125
5.541AlaGlu: 5.541 ± 0.099
3.386AlaPhe: 3.386 ± 0.072
8.893AlaGly: 8.893 ± 0.124
2.675AlaHis: 2.675 ± 0.073
5.388AlaIle: 5.388 ± 0.085
4.042AlaLys: 4.042 ± 0.099
10.828AlaLeu: 10.828 ± 0.148
3.711AlaMet: 3.711 ± 0.081
3.084AlaAsn: 3.084 ± 0.071
4.567AlaPro: 4.567 ± 0.085
5.194AlaGln: 5.194 ± 0.132
7.349AlaArg: 7.349 ± 0.124
5.938AlaSer: 5.938 ± 0.099
6.722AlaThr: 6.722 ± 0.113
9.008AlaVal: 9.008 ± 0.139
1.792AlaTrp: 1.792 ± 0.053
2.801AlaTyr: 2.801 ± 0.072
0.0AlaXaa: 0.0 ± 0.0
Cys
1.381CysAla: 1.381 ± 0.05
0.148CysCys: 0.148 ± 0.014
0.646CysAsp: 0.646 ± 0.033
0.509CysGlu: 0.509 ± 0.026
0.299CysPhe: 0.299 ± 0.019
1.057CysGly: 1.057 ± 0.039
0.235CysHis: 0.235 ± 0.019
0.475CysIle: 0.475 ± 0.031
0.247CysLys: 0.247 ± 0.02
0.851CysLeu: 0.851 ± 0.042
0.31CysMet: 0.31 ± 0.022
0.239CysAsn: 0.239 ± 0.018
0.524CysPro: 0.524 ± 0.028
0.254CysGln: 0.254 ± 0.019
0.701CysArg: 0.701 ± 0.035
0.614CysSer: 0.614 ± 0.031
0.697CysThr: 0.697 ± 0.034
0.844CysVal: 0.844 ± 0.041
0.155CysTrp: 0.155 ± 0.014
0.254CysTyr: 0.254 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
8.453AspAla: 8.453 ± 0.14
0.607AspCys: 0.607 ± 0.033
5.28AspAsp: 5.28 ± 0.108
4.843AspGlu: 4.843 ± 0.1
2.217AspPhe: 2.217 ± 0.061
7.171AspGly: 7.171 ± 0.111
1.461AspHis: 1.461 ± 0.049
3.122AspIle: 3.122 ± 0.068
2.009AspLys: 2.009 ± 0.063
5.857AspLeu: 5.857 ± 0.088
2.018AspMet: 2.018 ± 0.05
1.661AspAsn: 1.661 ± 0.06
4.019AspPro: 4.019 ± 0.083
2.01AspGln: 2.01 ± 0.063
4.247AspArg: 4.247 ± 0.076
3.089AspSer: 3.089 ± 0.078
4.067AspThr: 4.067 ± 0.074
5.346AspVal: 5.346 ± 0.103
1.182AspTrp: 1.182 ± 0.043
2.014AspTyr: 2.014 ± 0.06
0.0AspXaa: 0.0 ± 0.0
Glu
6.363GluAla: 6.363 ± 0.123
0.506GluCys: 0.506 ± 0.027
3.601GluAsp: 3.601 ± 0.091
3.714GluGlu: 3.714 ± 0.086
1.661GluPhe: 1.661 ± 0.051
4.04GluGly: 4.04 ± 0.082
1.856GluHis: 1.856 ± 0.055
2.434GluIle: 2.434 ± 0.056
1.64GluLys: 1.64 ± 0.06
5.367GluLeu: 5.367 ± 0.107
1.315GluMet: 1.315 ± 0.052
1.446GluAsn: 1.446 ± 0.046
2.69GluPro: 2.69 ± 0.072
2.939GluGln: 2.939 ± 0.064
5.04GluArg: 5.04 ± 0.1
2.725GluSer: 2.725 ± 0.062
3.096GluThr: 3.096 ± 0.065
3.736GluVal: 3.736 ± 0.064
0.723GluTrp: 0.723 ± 0.031
1.647GluTyr: 1.647 ± 0.05
0.0GluXaa: 0.0 ± 0.0
Phe
3.63PheAla: 3.63 ± 0.077
0.35PheCys: 0.35 ± 0.023
2.642PheAsp: 2.642 ± 0.053
1.729PheGlu: 1.729 ± 0.045
0.997PhePhe: 0.997 ± 0.05
2.835PheGly: 2.835 ± 0.067
0.698PheHis: 0.698 ± 0.034
1.392PheIle: 1.392 ± 0.05
0.933PheLys: 0.933 ± 0.038
2.739PheLeu: 2.739 ± 0.071
0.745PheMet: 0.745 ± 0.036
1.038PheAsn: 1.038 ± 0.04
1.37PhePro: 1.37 ± 0.054
0.829PheGln: 0.829 ± 0.037
1.766PheArg: 1.766 ± 0.049
1.889PheSer: 1.889 ± 0.057
2.225PheThr: 2.225 ± 0.06
2.465PheVal: 2.465 ± 0.071
0.393PheTrp: 0.393 ± 0.022
0.803PheTyr: 0.803 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
8.085GlyAla: 8.085 ± 0.124
0.884GlyCys: 0.884 ± 0.04
5.029GlyAsp: 5.029 ± 0.074
4.676GlyGlu: 4.676 ± 0.084
2.929GlyPhe: 2.929 ± 0.06
5.978GlyGly: 5.978 ± 0.115
1.892GlyHis: 1.892 ± 0.055
4.402GlyIle: 4.402 ± 0.085
3.399GlyLys: 3.399 ± 0.078
6.867GlyLeu: 6.867 ± 0.116
2.579GlyMet: 2.579 ± 0.06
2.529GlyAsn: 2.529 ± 0.083
2.726GlyPro: 2.726 ± 0.059
3.037GlyGln: 3.037 ± 0.068
5.201GlyArg: 5.201 ± 0.083
4.706GlySer: 4.706 ± 0.084
5.628GlyThr: 5.628 ± 0.109
6.381GlyVal: 6.381 ± 0.098
1.345GlyTrp: 1.345 ± 0.046
2.601GlyTyr: 2.601 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
2.763HisAla: 2.763 ± 0.076
0.246HisCys: 0.246 ± 0.021
1.941HisAsp: 1.941 ± 0.057
1.342HisGlu: 1.342 ± 0.051
0.524HisPhe: 0.524 ± 0.028
2.398HisGly: 2.398 ± 0.059
0.628HisHis: 0.628 ± 0.031
0.957HisIle: 0.957 ± 0.037
0.528HisLys: 0.528 ± 0.032
1.725HisLeu: 1.725 ± 0.054
0.628HisMet: 0.628 ± 0.03
0.578HisAsn: 0.578 ± 0.029
1.42HisPro: 1.42 ± 0.048
0.65HisGln: 0.65 ± 0.031
1.705HisArg: 1.705 ± 0.049
0.923HisSer: 0.923 ± 0.033
1.41HisThr: 1.41 ± 0.055
2.043HisVal: 2.043 ± 0.06
0.345HisTrp: 0.345 ± 0.023
0.618HisTyr: 0.618 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
5.753IleAla: 5.753 ± 0.104
0.549IleCys: 0.549 ± 0.028
3.74IleAsp: 3.74 ± 0.08
2.857IleGlu: 2.857 ± 0.07
1.171IlePhe: 1.171 ± 0.046
4.106IleGly: 4.106 ± 0.081
0.974IleHis: 0.974 ± 0.041
2.263IleIle: 2.263 ± 0.067
1.32IleLys: 1.32 ± 0.05
3.651IleLeu: 3.651 ± 0.082
1.202IleMet: 1.202 ± 0.047
1.427IleAsn: 1.427 ± 0.044
2.446IlePro: 2.446 ± 0.06
1.221IleGln: 1.221 ± 0.044
2.903IleArg: 2.903 ± 0.06
2.384IleSer: 2.384 ± 0.055
2.779IleThr: 2.779 ± 0.062
4.273IleVal: 4.273 ± 0.088
0.508IleTrp: 0.508 ± 0.031
0.999IleTyr: 0.999 ± 0.037
0.0IleXaa: 0.0 ± 0.0
Lys
3.957LysAla: 3.957 ± 0.11
0.164LysCys: 0.164 ± 0.013
2.387LysAsp: 2.387 ± 0.075
1.937LysGlu: 1.937 ± 0.068
0.749LysPhe: 0.749 ± 0.037
2.49LysGly: 2.49 ± 0.07
0.694LysHis: 0.694 ± 0.034
1.301LysIle: 1.301 ± 0.048
1.264LysLys: 1.264 ± 0.06
2.685LysLeu: 2.685 ± 0.068
0.762LysMet: 0.762 ± 0.033
0.951LysAsn: 0.951 ± 0.045
1.89LysPro: 1.89 ± 0.058
1.29LysGln: 1.29 ± 0.049
2.112LysArg: 2.112 ± 0.056
1.638LysSer: 1.638 ± 0.057
2.043LysThr: 2.043 ± 0.063
2.534LysVal: 2.534 ± 0.077
0.401LysTrp: 0.401 ± 0.023
0.938LysTyr: 0.938 ± 0.041
0.0LysXaa: 0.0 ± 0.0
Leu
11.178LeuAla: 11.178 ± 0.158
1.047LeuCys: 1.047 ± 0.043
6.824LeuAsp: 6.824 ± 0.112
4.934LeuGlu: 4.934 ± 0.095
2.929LeuPhe: 2.929 ± 0.074
7.195LeuGly: 7.195 ± 0.116
2.103LeuHis: 2.103 ± 0.055
3.794LeuIle: 3.794 ± 0.086
2.901LeuLys: 2.901 ± 0.076
7.939LeuLeu: 7.939 ± 0.152
2.366LeuMet: 2.366 ± 0.061
2.443LeuAsn: 2.443 ± 0.069
4.66LeuPro: 4.66 ± 0.089
2.747LeuGln: 2.747 ± 0.067
5.854LeuArg: 5.854 ± 0.108
4.949LeuSer: 4.949 ± 0.1
5.339LeuThr: 5.339 ± 0.095
7.009LeuVal: 7.009 ± 0.127
1.049LeuTrp: 1.049 ± 0.046
1.958LeuTyr: 1.958 ± 0.057
0.0LeuXaa: 0.0 ± 0.0
Met
3.499MetAla: 3.499 ± 0.063
0.302MetCys: 0.302 ± 0.02
1.807MetAsp: 1.807 ± 0.049
1.393MetGlu: 1.393 ± 0.046
0.84MetPhe: 0.84 ± 0.035
2.094MetGly: 2.094 ± 0.049
0.603MetHis: 0.603 ± 0.027
1.06MetIle: 1.06 ± 0.038
0.842MetLys: 0.842 ± 0.035
2.632MetLeu: 2.632 ± 0.06
0.72MetMet: 0.72 ± 0.032
0.869MetAsn: 0.869 ± 0.035
1.582MetPro: 1.582 ± 0.048
0.997MetGln: 0.997 ± 0.038
2.049MetArg: 2.049 ± 0.062
1.766MetSer: 1.766 ± 0.039
1.905MetThr: 1.905 ± 0.046
2.158MetVal: 2.158 ± 0.049
0.297MetTrp: 0.297 ± 0.022
0.512MetTyr: 0.512 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
3.231AsnAla: 3.231 ± 0.085
0.241AsnCys: 0.241 ± 0.019
1.798AsnAsp: 1.798 ± 0.058
1.595AsnGlu: 1.595 ± 0.051
0.793AsnPhe: 0.793 ± 0.034
2.839AsnGly: 2.839 ± 0.075
0.59AsnHis: 0.59 ± 0.028
1.273AsnIle: 1.273 ± 0.048
0.898AsnLys: 0.898 ± 0.039
2.294AsnLeu: 2.294 ± 0.065
0.737AsnMet: 0.737 ± 0.031
0.825AsnAsn: 0.825 ± 0.043
1.973AsnPro: 1.973 ± 0.051
0.964AsnGln: 0.964 ± 0.035
1.813AsnArg: 1.813 ± 0.053
1.271AsnSer: 1.271 ± 0.049
1.727AsnThr: 1.727 ± 0.055
2.038AsnVal: 2.038 ± 0.055
0.441AsnTrp: 0.441 ± 0.026
0.782AsnTyr: 0.782 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
5.581ProAla: 5.581 ± 0.105
0.366ProCys: 0.366 ± 0.025
4.023ProAsp: 4.023 ± 0.086
3.188ProGlu: 3.188 ± 0.064
1.54ProPhe: 1.54 ± 0.043
3.809ProGly: 3.809 ± 0.075
1.115ProHis: 1.115 ± 0.05
2.009ProIle: 2.009 ± 0.056
1.54ProLys: 1.54 ± 0.047
4.008ProLeu: 4.008 ± 0.08
1.29ProMet: 1.29 ± 0.044
1.409ProAsn: 1.409 ± 0.044
1.555ProPro: 1.555 ± 0.054
2.027ProGln: 2.027 ± 0.066
2.808ProArg: 2.808 ± 0.069
2.817ProSer: 2.817 ± 0.069
3.31ProThr: 3.31 ± 0.077
4.055ProVal: 4.055 ± 0.078
0.811ProTrp: 0.811 ± 0.037
1.472ProTyr: 1.472 ± 0.05
0.0ProXaa: 0.0 ± 0.0
Gln
4.618GlnAla: 4.618 ± 0.116
0.368GlnCys: 0.368 ± 0.022
2.252GlnAsp: 2.252 ± 0.055
2.264GlnGlu: 2.264 ± 0.063
1.057GlnPhe: 1.057 ± 0.038
2.704GlnGly: 2.704 ± 0.054
0.831GlnHis: 0.831 ± 0.037
1.57GlnIle: 1.57 ± 0.044
0.883GlnLys: 0.883 ± 0.039
3.695GlnLeu: 3.695 ± 0.075
1.061GlnMet: 1.061 ± 0.041
0.817GlnAsn: 0.817 ± 0.038
2.028GlnPro: 2.028 ± 0.069
1.73GlnGln: 1.73 ± 0.06
2.474GlnArg: 2.474 ± 0.059
1.88GlnSer: 1.88 ± 0.048
1.799GlnThr: 1.799 ± 0.059
3.245GlnVal: 3.245 ± 0.081
0.749GlnTrp: 0.749 ± 0.036
1.177GlnTyr: 1.177 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
6.479ArgAla: 6.479 ± 0.11
0.589ArgCys: 0.589 ± 0.032
4.219ArgAsp: 4.219 ± 0.086
3.887ArgGlu: 3.887 ± 0.094
2.475ArgPhe: 2.475 ± 0.064
4.171ArgGly: 4.171 ± 0.085
1.882ArgHis: 1.882 ± 0.064
3.802ArgIle: 3.802 ± 0.077
2.453ArgLys: 2.453 ± 0.06
6.273ArgLeu: 6.273 ± 0.111
2.148ArgMet: 2.148 ± 0.06
1.978ArgAsn: 1.978 ± 0.058
2.992ArgPro: 2.992 ± 0.076
2.936ArgGln: 2.936 ± 0.074
5.486ArgArg: 5.486 ± 0.138
3.213ArgSer: 3.213 ± 0.078
4.059ArgThr: 4.059 ± 0.075
4.551ArgVal: 4.551 ± 0.086
0.997ArgTrp: 0.997 ± 0.043
1.949ArgTyr: 1.949 ± 0.049
0.0ArgXaa: 0.0 ± 0.0
Ser
5.36SerAla: 5.36 ± 0.097
0.515SerCys: 0.515 ± 0.028
3.306SerAsp: 3.306 ± 0.078
2.511SerGlu: 2.511 ± 0.064
1.857SerPhe: 1.857 ± 0.052
4.705SerGly: 4.705 ± 0.09
1.191SerHis: 1.191 ± 0.041
2.728SerIle: 2.728 ± 0.066
1.707SerLys: 1.707 ± 0.051
4.757SerLeu: 4.757 ± 0.094
1.714SerMet: 1.714 ± 0.049
1.56SerAsn: 1.56 ± 0.048
2.548SerPro: 2.548 ± 0.065
1.865SerGln: 1.865 ± 0.054
3.422SerArg: 3.422 ± 0.067
3.217SerSer: 3.217 ± 0.088
3.359SerThr: 3.359 ± 0.076
4.132SerVal: 4.132 ± 0.095
0.909SerTrp: 0.909 ± 0.041
1.542SerTyr: 1.542 ± 0.051
0.0SerXaa: 0.0 ± 0.0
Thr
6.824ThrAla: 6.824 ± 0.112
0.631ThrCys: 0.631 ± 0.032
4.229ThrAsp: 4.229 ± 0.092
2.659ThrGlu: 2.659 ± 0.069
1.893ThrPhe: 1.893 ± 0.062
5.252ThrGly: 5.252 ± 0.088
1.366ThrHis: 1.366 ± 0.049
3.147ThrIle: 3.147 ± 0.068
1.918ThrLys: 1.918 ± 0.067
5.841ThrLeu: 5.841 ± 0.102
1.72ThrMet: 1.72 ± 0.05
1.716ThrAsn: 1.716 ± 0.053
3.64ThrPro: 3.64 ± 0.089
2.17ThrGln: 2.17 ± 0.058
3.42ThrArg: 3.42 ± 0.086
3.157ThrSer: 3.157 ± 0.071
3.735ThrThr: 3.735 ± 0.095
5.814ThrVal: 5.814 ± 0.118
0.844ThrTrp: 0.844 ± 0.034
1.668ThrTyr: 1.668 ± 0.049
0.0ThrXaa: 0.0 ± 0.0
Val
8.804ValAla: 8.804 ± 0.132
0.982ValCys: 0.982 ± 0.034
5.906ValAsp: 5.906 ± 0.116
4.63ValGlu: 4.63 ± 0.102
2.712ValPhe: 2.712 ± 0.066
5.599ValGly: 5.599 ± 0.106
1.664ValHis: 1.664 ± 0.057
3.769ValIle: 3.769 ± 0.083
2.454ValLys: 2.454 ± 0.069
7.33ValLeu: 7.33 ± 0.12
1.897ValMet: 1.897 ± 0.06
2.276ValAsn: 2.276 ± 0.064
4.216ValPro: 4.216 ± 0.089
2.631ValGln: 2.631 ± 0.063
5.067ValArg: 5.067 ± 0.097
4.615ValSer: 4.615 ± 0.085
5.406ValThr: 5.406 ± 0.108
6.705ValVal: 6.705 ± 0.12
1.042ValTrp: 1.042 ± 0.036
1.734ValTyr: 1.734 ± 0.049
0.0ValXaa: 0.0 ± 0.0
Trp
1.353TrpAla: 1.353 ± 0.042
0.225TrpCys: 0.225 ± 0.017
0.949TrpAsp: 0.949 ± 0.039
0.672TrpGlu: 0.672 ± 0.032
0.56TrpPhe: 0.56 ± 0.029
0.924TrpGly: 0.924 ± 0.037
0.413TrpHis: 0.413 ± 0.025
0.672TrpIle: 0.672 ± 0.033
0.517TrpLys: 0.517 ± 0.028
1.58TrpLeu: 1.58 ± 0.05
0.411TrpMet: 0.411 ± 0.023
0.595TrpAsn: 0.595 ± 0.035
0.611TrpPro: 0.611 ± 0.03
0.764TrpGln: 0.764 ± 0.032
1.118TrpArg: 1.118 ± 0.044
0.796TrpSer: 0.796 ± 0.037
0.844TrpThr: 0.844 ± 0.037
0.974TrpVal: 0.974 ± 0.039
0.37TrpTrp: 0.37 ± 0.027
0.426TrpTyr: 0.426 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.122TyrAla: 3.122 ± 0.067
0.315TyrCys: 0.315 ± 0.022
1.984TyrAsp: 1.984 ± 0.06
1.658TyrGlu: 1.658 ± 0.05
0.877TyrPhe: 0.877 ± 0.041
2.612TyrGly: 2.612 ± 0.067
0.505TyrHis: 0.505 ± 0.023
0.967TyrIle: 0.967 ± 0.04
0.691TyrLys: 0.691 ± 0.033
2.3TyrLeu: 2.3 ± 0.064
0.593TyrMet: 0.593 ± 0.028
0.686TyrAsn: 0.686 ± 0.036
1.272TyrPro: 1.272 ± 0.045
0.945TyrGln: 0.945 ± 0.036
2.007TyrArg: 2.007 ± 0.047
1.318TyrSer: 1.318 ± 0.045
1.493TyrThr: 1.493 ± 0.053
2.13TyrVal: 2.13 ± 0.055
0.421TyrTrp: 0.421 ± 0.026
0.712TyrTyr: 0.712 ± 0.038
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2189 proteins (724817 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski