Amino acid dipepetide frequency for Methylobacterium sp. Leaf399

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.235AlaAla: 21.235 ± 0.207	1.263AlaCys: 1.263 ± 0.036	7.786AlaAsp: 7.786 ± 0.101	7.993AlaGlu: 7.993 ± 0.1	4.725AlaPhe: 4.725 ± 0.068	13.101AlaGly: 13.101 ± 0.145	2.371AlaHis: 2.371 ± 0.05	6.411AlaIle: 6.411 ± 0.074	3.75AlaLys: 3.75 ± 0.082	15.268AlaLeu: 15.268 ± 0.151	3.617AlaMet: 3.617 ± 0.058	2.81AlaAsn: 2.81 ± 0.074	7.06AlaPro: 7.06 ± 0.109	4.085AlaGln: 4.085 ± 0.066	11.109AlaArg: 11.109 ± 0.14	6.842AlaSer: 6.842 ± 0.089	7.316AlaThr: 7.316 ± 0.087	10.578AlaVal: 10.578 ± 0.093	1.689AlaTrp: 1.689 ± 0.045	2.687AlaTyr: 2.687 ± 0.056	0.002AlaXaa: 0.002 ± 0.001
Cys	0.993CysAla: 0.993 ± 0.031	0.088CysCys: 0.088 ± 0.01	0.51CysAsp: 0.51 ± 0.021	0.422CysGlu: 0.422 ± 0.019	0.248CysPhe: 0.248 ± 0.015	0.884CysGly: 0.884 ± 0.034	0.182CysHis: 0.182 ± 0.012	0.324CysIle: 0.324 ± 0.017	0.147CysLys: 0.147 ± 0.011	0.915CysLeu: 0.915 ± 0.026	0.136CysMet: 0.136 ± 0.011	0.177CysAsn: 0.177 ± 0.011	0.441CysPro: 0.441 ± 0.022	0.182CysGln: 0.182 ± 0.012	0.707CysArg: 0.707 ± 0.027	0.349CysSer: 0.349 ± 0.017	0.41CysThr: 0.41 ± 0.02	0.544CysVal: 0.544 ± 0.021	0.09CysTrp: 0.09 ± 0.009	0.132CysTyr: 0.132 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	8.062AspAla: 8.062 ± 0.091	0.43AspCys: 0.43 ± 0.02	3.297AspAsp: 3.297 ± 0.068	3.252AspGlu: 3.252 ± 0.042	1.833AspPhe: 1.833 ± 0.046	6.223AspGly: 6.223 ± 0.122	1.238AspHis: 1.238 ± 0.031	2.418AspIle: 2.418 ± 0.045	1.337AspLys: 1.337 ± 0.04	7.069AspLeu: 7.069 ± 0.087	0.981AspMet: 0.981 ± 0.028	0.961AspAsn: 0.961 ± 0.034	4.069AspPro: 4.069 ± 0.059	1.359AspGln: 1.359 ± 0.039	5.194AspArg: 5.194 ± 0.069	1.849AspSer: 1.849 ± 0.044	2.909AspThr: 2.909 ± 0.09	4.516AspVal: 4.516 ± 0.069	0.821AspTrp: 0.821 ± 0.026	1.28AspTyr: 1.28 ± 0.033	0.001AspXaa: 0.001 ± 0.001
Glu	9.157GluAla: 9.157 ± 0.116	0.287GluCys: 0.287 ± 0.015	2.576GluAsp: 2.576 ± 0.052	2.63GluGlu: 2.63 ± 0.056	1.315GluPhe: 1.315 ± 0.036	4.741GluGly: 4.741 ± 0.061	0.964GluHis: 0.964 ± 0.03	3.058GluIle: 3.058 ± 0.054	1.433GluLys: 1.433 ± 0.043	3.82GluLeu: 3.82 ± 0.072	1.175GluMet: 1.175 ± 0.036	1.149GluAsn: 1.149 ± 0.035	2.992GluPro: 2.992 ± 0.063	1.454GluGln: 1.454 ± 0.04	5.367GluArg: 5.367 ± 0.083	2.181GluSer: 2.181 ± 0.045	3.735GluThr: 3.735 ± 0.061	3.823GluVal: 3.823 ± 0.056	0.549GluTrp: 0.549 ± 0.021	0.719GluTyr: 0.719 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	4.577PheAla: 4.577 ± 0.067	0.318PheCys: 0.318 ± 0.016	2.39PheAsp: 2.39 ± 0.046	1.876PheGlu: 1.876 ± 0.037	1.132PhePhe: 1.132 ± 0.031	3.484PheGly: 3.484 ± 0.06	0.621PheHis: 0.621 ± 0.025	1.092PheIle: 1.092 ± 0.03	0.883PheLys: 0.883 ± 0.03	3.033PheLeu: 3.033 ± 0.049	0.599PheMet: 0.599 ± 0.023	0.784PheAsn: 0.784 ± 0.027	1.441PhePro: 1.441 ± 0.035	0.788PheGln: 0.788 ± 0.029	2.182PheArg: 2.182 ± 0.042	2.022PheSer: 2.022 ± 0.047	1.961PheThr: 1.961 ± 0.044	2.85PheVal: 2.85 ± 0.051	0.414PheTrp: 0.414 ± 0.019	0.762PheTyr: 0.762 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	11.292GlyAla: 11.292 ± 0.143	0.804GlyCys: 0.804 ± 0.025	5.028GlyAsp: 5.028 ± 0.099	4.844GlyGlu: 4.844 ± 0.068	3.638GlyPhe: 3.638 ± 0.051	8.397GlyGly: 8.397 ± 0.164	1.897GlyHis: 1.897 ± 0.04	4.499GlyIle: 4.499 ± 0.064	2.515GlyLys: 2.515 ± 0.06	10.438GlyLeu: 10.438 ± 0.121	1.926GlyMet: 1.926 ± 0.045	2.081GlyAsn: 2.081 ± 0.105	4.473GlyPro: 4.473 ± 0.072	2.835GlyGln: 2.835 ± 0.053	8.031GlyArg: 8.031 ± 0.098	4.787GlySer: 4.787 ± 0.089	5.606GlyThr: 5.606 ± 0.113	6.175GlyVal: 6.175 ± 0.074	1.266GlyTrp: 1.266 ± 0.04	2.227GlyTyr: 2.227 ± 0.054	0.004GlyXaa: 0.004 ± 0.002
His	2.542HisAla: 2.542 ± 0.048	0.194HisCys: 0.194 ± 0.013	1.244HisAsp: 1.244 ± 0.031	0.904HisGlu: 0.904 ± 0.026	0.634HisPhe: 0.634 ± 0.024	1.991HisGly: 1.991 ± 0.051	0.578HisHis: 0.578 ± 0.028	0.653HisIle: 0.653 ± 0.025	0.333HisLys: 0.333 ± 0.016	2.159HisLeu: 2.159 ± 0.05	0.333HisMet: 0.333 ± 0.017	0.393HisAsn: 0.393 ± 0.018	1.367HisPro: 1.367 ± 0.037	0.457HisGln: 0.457 ± 0.019	1.578HisArg: 1.578 ± 0.035	0.652HisSer: 0.652 ± 0.024	0.685HisThr: 0.685 ± 0.023	1.478HisVal: 1.478 ± 0.034	0.262HisTrp: 0.262 ± 0.014	0.454HisTyr: 0.454 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	7.112IleAla: 7.112 ± 0.071	0.404IleCys: 0.404 ± 0.02	3.203IleAsp: 3.203 ± 0.053	3.221IleGlu: 3.221 ± 0.059	1.162IlePhe: 1.162 ± 0.034	4.65IleGly: 4.65 ± 0.07	0.766IleHis: 0.766 ± 0.027	1.306IleIle: 1.306 ± 0.042	1.077IleLys: 1.077 ± 0.033	4.187IleLeu: 4.187 ± 0.061	0.638IleMet: 0.638 ± 0.02	0.985IleAsn: 0.985 ± 0.033	2.099IlePro: 2.099 ± 0.042	1.05IleGln: 1.05 ± 0.031	3.106IleArg: 3.106 ± 0.053	1.91IleSer: 1.91 ± 0.042	2.335IleThr: 2.335 ± 0.05	4.079IleVal: 4.079 ± 0.07	0.427IleTrp: 0.427 ± 0.021	0.814IleTyr: 0.814 ± 0.025	0.001IleXaa: 0.001 ± 0.001
Lys	3.938LysAla: 3.938 ± 0.082	0.103LysCys: 0.103 ± 0.01	1.458LysAsp: 1.458 ± 0.038	1.107LysGlu: 1.107 ± 0.037	0.576LysPhe: 0.576 ± 0.02	2.322LysGly: 2.322 ± 0.042	0.373LysHis: 0.373 ± 0.017	1.172LysIle: 1.172 ± 0.04	0.818LysLys: 0.818 ± 0.033	2.305LysLeu: 2.305 ± 0.052	0.493LysMet: 0.493 ± 0.02	0.607LysAsn: 0.607 ± 0.025	1.899LysPro: 1.899 ± 0.049	0.642LysGln: 0.642 ± 0.025	2.026LysArg: 2.026 ± 0.045	1.344LysSer: 1.344 ± 0.04	1.512LysThr: 1.512 ± 0.037	2.016LysVal: 2.016 ± 0.048	0.217LysTrp: 0.217 ± 0.013	0.391LysTyr: 0.391 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	16.004LeuAla: 16.004 ± 0.133	0.921LeuCys: 0.921 ± 0.029	6.884LeuAsp: 6.884 ± 0.085	4.365LeuGlu: 4.365 ± 0.071	3.21LeuPhe: 3.21 ± 0.055	8.783LeuGly: 8.783 ± 0.091	1.827LeuHis: 1.827 ± 0.045	4.153LeuIle: 4.153 ± 0.06	2.861LeuLys: 2.861 ± 0.061	8.732LeuLeu: 8.732 ± 0.1	2.062LeuMet: 2.062 ± 0.046	2.142LeuAsn: 2.142 ± 0.047	5.749LeuPro: 5.749 ± 0.068	2.309LeuGln: 2.309 ± 0.043	7.704LeuArg: 7.704 ± 0.089	6.354LeuSer: 6.354 ± 0.086	5.975LeuThr: 5.975 ± 0.084	8.78LeuVal: 8.78 ± 0.096	1.063LeuTrp: 1.063 ± 0.032	1.922LeuTyr: 1.922 ± 0.045	0.002LeuXaa: 0.002 ± 0.002
Met	2.856MetAla: 2.856 ± 0.045	0.116MetCys: 0.116 ± 0.012	0.892MetAsp: 0.892 ± 0.03	0.934MetGlu: 0.934 ± 0.028	0.471MetPhe: 0.471 ± 0.017	1.578MetGly: 1.578 ± 0.043	0.354MetHis: 0.354 ± 0.018	1.071MetIle: 1.071 ± 0.028	0.658MetLys: 0.658 ± 0.024	2.001MetLeu: 2.001 ± 0.043	0.514MetMet: 0.514 ± 0.022	0.566MetAsn: 0.566 ± 0.02	1.51MetPro: 1.51 ± 0.041	0.656MetGln: 0.656 ± 0.024	1.826MetArg: 1.826 ± 0.04	1.349MetSer: 1.349 ± 0.031	1.712MetThr: 1.712 ± 0.032	1.416MetVal: 1.416 ± 0.032	0.138MetTrp: 0.138 ± 0.011	0.178MetTyr: 0.178 ± 0.011	0.001MetXaa: 0.001 ± 0.001
Asn	2.893AsnAla: 2.893 ± 0.054	0.172AsnCys: 0.172 ± 0.01	1.258AsnAsp: 1.258 ± 0.05	0.98AsnGlu: 0.98 ± 0.026	0.656AsnPhe: 0.656 ± 0.026	2.186AsnGly: 2.186 ± 0.071	0.384AsnHis: 0.384 ± 0.017	0.865AsnIle: 0.865 ± 0.031	0.475AsnLys: 0.475 ± 0.023	2.238AsnLeu: 2.238 ± 0.054	0.376AsnMet: 0.376 ± 0.021	0.499AsnAsn: 0.499 ± 0.025	1.57AsnPro: 1.57 ± 0.04	0.57AsnGln: 0.57 ± 0.024	1.656AsnArg: 1.656 ± 0.039	0.832AsnSer: 0.832 ± 0.036	1.088AsnThr: 1.088 ± 0.037	1.716AsnVal: 1.716 ± 0.048	0.256AsnTrp: 0.256 ± 0.016	0.475AsnTyr: 0.475 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	8.129ProAla: 8.129 ± 0.132	0.342ProCys: 0.342 ± 0.018	4.121ProAsp: 4.121 ± 0.069	3.781ProGlu: 3.781 ± 0.064	2.047ProPhe: 2.047 ± 0.042	5.491ProGly: 5.491 ± 0.089	1.038ProHis: 1.038 ± 0.029	2.228ProIle: 2.228 ± 0.044	1.437ProLys: 1.437 ± 0.038	4.849ProLeu: 4.849 ± 0.067	1.198ProMet: 1.198 ± 0.036	1.129ProAsn: 1.129 ± 0.031	3.254ProPro: 3.254 ± 0.088	1.476ProGln: 1.476 ± 0.03	3.783ProArg: 3.783 ± 0.064	2.979ProSer: 2.979 ± 0.051	2.722ProThr: 2.722 ± 0.055	4.751ProVal: 4.751 ± 0.079	0.733ProTrp: 0.733 ± 0.022	1.06ProTyr: 1.06 ± 0.029	0.002ProXaa: 0.002 ± 0.001
Gln	4.449GlnAla: 4.449 ± 0.075	0.167GlnCys: 0.167 ± 0.013	1.398GlnAsp: 1.398 ± 0.037	1.221GlnGlu: 1.221 ± 0.037	0.831GlnPhe: 0.831 ± 0.025	2.528GlnGly: 2.528 ± 0.05	0.443GlnHis: 0.443 ± 0.019	1.437GlnIle: 1.437 ± 0.04	0.727GlnLys: 0.727 ± 0.027	2.051GlnLeu: 2.051 ± 0.045	0.581GlnMet: 0.581 ± 0.021	0.608GlnAsn: 0.608 ± 0.025	1.509GlnPro: 1.509 ± 0.039	0.772GlnGln: 0.772 ± 0.033	2.158GlnArg: 2.158 ± 0.053	1.411GlnSer: 1.411 ± 0.04	1.607GlnThr: 1.607 ± 0.04	2.172GlnVal: 2.172 ± 0.051	0.271GlnTrp: 0.271 ± 0.014	0.438GlnTyr: 0.438 ± 0.02	0.002GlnXaa: 0.002 ± 0.001
Arg	9.806ArgAla: 9.806 ± 0.113	0.522ArgCys: 0.522 ± 0.022	4.805ArgAsp: 4.805 ± 0.073	4.339ArgGlu: 4.339 ± 0.072	3.134ArgPhe: 3.134 ± 0.062	5.73ArgGly: 5.73 ± 0.083	1.863ArgHis: 1.863 ± 0.046	4.388ArgIle: 4.388 ± 0.069	1.769ArgLys: 1.769 ± 0.042	9.272ArgLeu: 9.272 ± 0.103	1.828ArgMet: 1.828 ± 0.042	1.6ArgAsn: 1.6 ± 0.036	4.726ArgPro: 4.726 ± 0.085	2.371ArgGln: 2.371 ± 0.054	7.227ArgArg: 7.227 ± 0.101	4.122ArgSer: 4.122 ± 0.063	4.184ArgThr: 4.184 ± 0.073	5.585ArgVal: 5.585 ± 0.076	0.993ArgTrp: 0.993 ± 0.031	1.711ArgTyr: 1.711 ± 0.041	0.002ArgXaa: 0.002 ± 0.001
Ser	6.287SerAla: 6.287 ± 0.086	0.33SerCys: 0.33 ± 0.019	2.893SerAsp: 2.893 ± 0.056	2.542SerGlu: 2.542 ± 0.051	1.888SerPhe: 1.888 ± 0.039	5.475SerGly: 5.475 ± 0.086	0.94SerHis: 0.94 ± 0.029	2.124SerIle: 2.124 ± 0.048	1.128SerLys: 1.128 ± 0.035	5.5SerLeu: 5.5 ± 0.067	0.947SerMet: 0.947 ± 0.03	1.065SerAsn: 1.065 ± 0.041	2.894SerPro: 2.894 ± 0.059	1.395SerGln: 1.395 ± 0.035	3.794SerArg: 3.794 ± 0.064	2.366SerSer: 2.366 ± 0.049	2.552SerThr: 2.552 ± 0.049	3.96SerVal: 3.96 ± 0.078	0.577SerTrp: 0.577 ± 0.026	1.15SerTyr: 1.15 ± 0.038	0.001SerXaa: 0.001 ± 0.001
Thr	7.193ThrAla: 7.193 ± 0.092	0.419ThrCys: 0.419 ± 0.022	2.993ThrAsp: 2.993 ± 0.06	2.479ThrGlu: 2.479 ± 0.05	1.941ThrPhe: 1.941 ± 0.048	6.008ThrGly: 6.008 ± 0.114	0.983ThrHis: 0.983 ± 0.031	2.776ThrIle: 2.776 ± 0.047	1.301ThrLys: 1.301 ± 0.038	6.514ThrLeu: 6.514 ± 0.088	1.084ThrMet: 1.084 ± 0.031	1.154ThrAsn: 1.154 ± 0.035	3.522ThrPro: 3.522 ± 0.061	1.466ThrGln: 1.466 ± 0.039	3.778ThrArg: 3.778 ± 0.061	2.515ThrSer: 2.515 ± 0.053	3.134ThrThr: 3.134 ± 0.068	5.006ThrVal: 5.006 ± 0.07	0.694ThrTrp: 0.694 ± 0.024	1.214ThrTyr: 1.214 ± 0.037	0.002ThrXaa: 0.002 ± 0.001
Val	11.365ValAla: 11.365 ± 0.113	0.647ValCys: 0.647 ± 0.024	4.481ValAsp: 4.481 ± 0.075	4.68ValGlu: 4.68 ± 0.069	2.63ValPhe: 2.63 ± 0.048	6.167ValGly: 6.167 ± 0.074	1.379ValHis: 1.379 ± 0.037	3.439ValIle: 3.439 ± 0.065	1.94ValLys: 1.94 ± 0.046	7.95ValLeu: 7.95 ± 0.091	1.734ValMet: 1.734 ± 0.032	1.668ValAsn: 1.668 ± 0.048	4.396ValPro: 4.396 ± 0.063	2.009ValGln: 2.009 ± 0.042	5.681ValArg: 5.681 ± 0.071	4.322ValSer: 4.322 ± 0.06	4.969ValThr: 4.969 ± 0.076	7.316ValVal: 7.316 ± 0.094	0.838ValTrp: 0.838 ± 0.028	1.358ValTyr: 1.358 ± 0.033	0.001ValXaa: 0.001 ± 0.001
Trp	1.177TrpAla: 1.177 ± 0.034	0.14TrpCys: 0.14 ± 0.01	0.552TrpAsp: 0.552 ± 0.021	0.456TrpGlu: 0.456 ± 0.022	0.451TrpPhe: 0.451 ± 0.021	0.803TrpGly: 0.803 ± 0.029	0.288TrpHis: 0.288 ± 0.014	0.556TrpIle: 0.556 ± 0.022	0.313TrpLys: 0.313 ± 0.017	1.446TrpLeu: 1.446 ± 0.041	0.276TrpMet: 0.276 ± 0.017	0.317TrpAsn: 0.317 ± 0.015	0.651TrpPro: 0.651 ± 0.025	0.4TrpGln: 0.4 ± 0.019	1.227TrpArg: 1.227 ± 0.036	0.774TrpSer: 0.774 ± 0.025	0.793TrpThr: 0.793 ± 0.028	0.698TrpVal: 0.698 ± 0.027	0.217TrpTrp: 0.217 ± 0.014	0.24TrpTyr: 0.24 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.627TyrAla: 2.627 ± 0.055	0.213TyrCys: 0.213 ± 0.012	1.325TyrAsp: 1.325 ± 0.031	1.101TyrGlu: 1.101 ± 0.03	0.689TyrPhe: 0.689 ± 0.021	2.217TyrGly: 2.217 ± 0.048	0.4TyrHis: 0.4 ± 0.018	0.564TyrIle: 0.564 ± 0.024	0.419TyrLys: 0.419 ± 0.021	2.057TyrLeu: 2.057 ± 0.047	0.298TyrMet: 0.298 ± 0.016	0.453TyrAsn: 0.453 ± 0.025	0.928TyrPro: 0.928 ± 0.032	0.5TyrGln: 0.5 ± 0.02	1.841TyrArg: 1.841 ± 0.042	0.846TyrSer: 0.846 ± 0.032	0.969TyrThr: 0.969 ± 0.033	1.506TyrVal: 1.506 ± 0.036	0.26TyrTrp: 0.26 ± 0.014	0.466TyrTyr: 0.466 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.002XaaAsp: 0.002 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.004XaaGly: 0.004 ± 0.004	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.004XaaArg: 0.004 ± 0.002	0.002XaaSer: 0.002 ± 0.001	0.002XaaThr: 0.002 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3885 proteins (1232780 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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