Trametes coccinea BRFM310

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Polyporaceae; Trametes; Trametes coccinea

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12648 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y2IP22|A0A1Y2IP22_PYCCO CRAL/TRIO domain-containing protein OS=Trametes coccinea BRFM310 OX=1353009 GN=PYCCODRAFT_1477715 PE=4 SV=1
MM1 pKa = 7.76CSLKK5 pKa = 10.6PSVSAALAASASATGVSGAASGSSSSSSSGNSSVGDD41 pKa = 3.39EE42 pKa = 4.44VVASTWYY49 pKa = 10.52AGWHH53 pKa = 6.25GEE55 pKa = 4.3DD56 pKa = 4.59FPPEE60 pKa = 4.21NISWSKK66 pKa = 8.18YY67 pKa = 6.24TQVTYY72 pKa = 11.12AFAVTTPDD80 pKa = 3.16VNTVSLEE87 pKa = 4.02ASDD90 pKa = 5.22EE91 pKa = 4.1EE92 pKa = 4.3LLPRR96 pKa = 11.84FVNAAHH102 pKa = 5.82QNNVKK107 pKa = 10.73AMLTVGGWTGSQYY120 pKa = 10.5FSSAVATEE128 pKa = 4.03ANRR131 pKa = 11.84TAFVKK136 pKa = 9.95TVLGLVSKK144 pKa = 11.08YY145 pKa = 10.9NLDD148 pKa = 3.58GLDD151 pKa = 4.4FDD153 pKa = 4.25WEE155 pKa = 4.5YY156 pKa = 10.9PNKK159 pKa = 10.16QGIGCNLISADD170 pKa = 3.91DD171 pKa = 3.89SQNFLSFLQALRR183 pKa = 11.84ADD185 pKa = 3.79PAGQNLTLSAAAGITPFAGPDD206 pKa = 3.73GTPMSDD212 pKa = 2.73VSDD215 pKa = 3.9FAKK218 pKa = 10.85VLDD221 pKa = 4.03YY222 pKa = 10.98VAIMNYY228 pKa = 9.64DD229 pKa = 2.97VWGSWSSSVGPNAPLNDD246 pKa = 3.78TCASAADD253 pKa = 3.86QQGSAVSAVKK263 pKa = 10.08AWTGAGFPANQLVLGVASYY282 pKa = 10.41GHH284 pKa = 6.2SFSVSNDD291 pKa = 2.84AALSGNTLLPYY302 pKa = 10.16PAFNKK307 pKa = 10.12ANQPLGDD314 pKa = 3.85KK315 pKa = 9.64WDD317 pKa = 3.64TDD319 pKa = 3.69AAPGVDD325 pKa = 3.15QCGNPTGGPSGIFDD339 pKa = 4.06FFGLIDD345 pKa = 4.34SKK347 pKa = 11.27FLTANGTVADD357 pKa = 4.96GIDD360 pKa = 3.45YY361 pKa = 10.92RR362 pKa = 11.84FDD364 pKa = 3.45EE365 pKa = 5.16CSQTPYY371 pKa = 10.61VYY373 pKa = 10.71NPSTQVMVSYY383 pKa = 11.44DD384 pKa = 3.68DD385 pKa = 3.74ATSFAAKK392 pKa = 10.22GKK394 pKa = 9.8FINDD398 pKa = 3.14AGLLGFAMWEE408 pKa = 3.97AAGDD412 pKa = 4.11SNDD415 pKa = 3.43ILLDD419 pKa = 4.56AIEE422 pKa = 3.89QAIGFEE428 pKa = 4.46EE429 pKa = 4.51VDD431 pKa = 3.79CC432 pKa = 5.43

Molecular weight: 45.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y2IP82|A0A1Y2IP82_PYCCO Uncharacterized protein OS=Trametes coccinea BRFM310 OX=1353009 GN=PYCCODRAFT_1477236 PE=4 SV=1
MM1 pKa = 7.89PKK3 pKa = 9.88RR4 pKa = 11.84SARR7 pKa = 11.84KK8 pKa = 8.95RR9 pKa = 11.84RR10 pKa = 11.84LARR13 pKa = 11.84PSSRR17 pKa = 11.84SSSARR22 pKa = 11.84TTRR25 pKa = 11.84KK26 pKa = 9.15RR27 pKa = 11.84MKK29 pKa = 8.8RR30 pKa = 11.84TRR32 pKa = 11.84MTKK35 pKa = 10.27KK36 pKa = 8.4MWRR39 pKa = 11.84LLPSRR44 pKa = 11.84SRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84AVASEE53 pKa = 3.48RR54 pKa = 11.84HH55 pKa = 5.53RR56 pKa = 11.84PQSRR60 pKa = 11.84PEE62 pKa = 3.76RR63 pKa = 11.84RR64 pKa = 11.84WRR66 pKa = 11.84RR67 pKa = 11.84RR68 pKa = 11.84WMRR71 pKa = 11.84TSTTSWRR78 pKa = 11.84TAMEE82 pKa = 4.32KK83 pKa = 9.88GTT85 pKa = 3.89

Molecular weight: 10.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12647

1

12648

5438953

39

5057

430.0

47.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.644 ± 0.022

1.284 ± 0.009

5.495 ± 0.014

5.886 ± 0.023

3.479 ± 0.014

6.564 ± 0.019

2.607 ± 0.011

4.344 ± 0.015

4.104 ± 0.022

9.103 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.034 ± 0.008

2.982 ± 0.012

7.089 ± 0.032

3.715 ± 0.016

6.825 ± 0.021

8.508 ± 0.036

5.853 ± 0.014

6.436 ± 0.017

1.431 ± 0.009

2.619 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski