Pterula gracilis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Agaricales incertae sedis; Pterulaceae; Pterula

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12852 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C3QXB9|A0A5C3QXB9_9AGAR Uncharacterized protein OS=Pterula gracilis OX=1884261 GN=BDV98DRAFT_647655 PE=4 SV=1
MM1 pKa = 6.63TTYY4 pKa = 10.53SHH6 pKa = 7.19HH7 pKa = 7.31LLTLDD12 pKa = 3.46EE13 pKa = 5.49RR14 pKa = 11.84IAAHH18 pKa = 6.88INALPVLAKK27 pKa = 10.55DD28 pKa = 5.39AITLDD33 pKa = 4.08DD34 pKa = 3.51TCAICFLPFSSAFDD48 pKa = 4.25DD49 pKa = 4.3EE50 pKa = 4.94PLPSSTVDD58 pKa = 3.47SDD60 pKa = 4.06VEE62 pKa = 4.27LNGITKK68 pKa = 9.72IVEE71 pKa = 4.4CGHH74 pKa = 5.11TFCRR78 pKa = 11.84KK79 pKa = 10.16DD80 pKa = 3.4LVQWIQSRR88 pKa = 11.84HH89 pKa = 4.32GTCPTCRR96 pKa = 11.84FPFLDD101 pKa = 3.55VRR103 pKa = 11.84PPSDD107 pKa = 3.9SDD109 pKa = 4.64DD110 pKa = 3.7EE111 pKa = 4.95SSDD114 pKa = 3.15GGEE117 pKa = 3.82YY118 pKa = 10.73VPDD121 pKa = 3.74YY122 pKa = 11.02EE123 pKa = 6.27HH124 pKa = 7.42DD125 pKa = 3.82LEE127 pKa = 5.84YY128 pKa = 9.91DD129 pKa = 3.2TDD131 pKa = 5.54GDD133 pKa = 3.97MDD135 pKa = 3.75WDD137 pKa = 3.86YY138 pKa = 11.95DD139 pKa = 3.49EE140 pKa = 6.65DD141 pKa = 3.9MDD143 pKa = 5.2IEE145 pKa = 4.52RR146 pKa = 11.84EE147 pKa = 4.1MMEE150 pKa = 4.33LDD152 pKa = 3.5ADD154 pKa = 4.16EE155 pKa = 5.54RR156 pKa = 11.84DD157 pKa = 3.64HH158 pKa = 7.68LHH160 pKa = 7.25DD161 pKa = 4.56YY162 pKa = 10.94EE163 pKa = 5.31PGSVGEE169 pKa = 4.4GEE171 pKa = 4.5GDD173 pKa = 3.79YY174 pKa = 11.32EE175 pKa = 4.48PGSVGHH181 pKa = 5.88YY182 pKa = 10.13AGLLIGEE189 pKa = 4.46SQAEE193 pKa = 4.06DD194 pKa = 3.63EE195 pKa = 4.6EE196 pKa = 5.38RR197 pKa = 11.84DD198 pKa = 3.52EE199 pKa = 4.24GRR201 pKa = 11.84MMGAAVGLVMAMDD214 pKa = 5.4AYY216 pKa = 10.74DD217 pKa = 3.81SSSDD221 pKa = 3.49EE222 pKa = 4.3SEE224 pKa = 3.91EE225 pKa = 4.03DD226 pKa = 3.47LLEE229 pKa = 4.28GEE231 pKa = 4.28IRR233 pKa = 11.84STEE236 pKa = 4.01VDD238 pKa = 3.35SHH240 pKa = 7.78DD241 pKa = 3.54GGTPGSYY248 pKa = 10.21AGEE251 pKa = 4.05EE252 pKa = 4.17PKK254 pKa = 10.93

Molecular weight: 28.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C3Q956|A0A5C3Q956_9AGAR Uncharacterized protein OS=Pterula gracilis OX=1884261 GN=BDV98DRAFT_608104 PE=4 SV=1
MM1 pKa = 7.16HH2 pKa = 7.56RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84GGSLGRR11 pKa = 11.84LVRR14 pKa = 11.84RR15 pKa = 11.84LLRR18 pKa = 11.84RR19 pKa = 11.84PPALRR24 pKa = 11.84LLFSTPPLPPLPLTPSTLRR43 pKa = 11.84HH44 pKa = 5.89SPTPKK49 pKa = 9.47PKK51 pKa = 10.21PMPRR55 pKa = 11.84KK56 pKa = 8.87SKK58 pKa = 9.31NWKK61 pKa = 9.32ISPLRR66 pKa = 11.84PVHH69 pKa = 6.3RR70 pKa = 11.84RR71 pKa = 11.84ARR73 pKa = 11.84SPSLSPFRR81 pKa = 11.84PPTLHH86 pKa = 5.81RR87 pKa = 11.84HH88 pKa = 5.24RR89 pKa = 11.84RR90 pKa = 11.84SSLLRR95 pKa = 11.84SPRR98 pKa = 11.84RR99 pKa = 11.84GVCMIRR105 pKa = 11.84RR106 pKa = 11.84GRR108 pKa = 11.84GGLAGGGFRR117 pKa = 11.84GRR119 pKa = 11.84GRR121 pKa = 11.84SLRR124 pKa = 11.84LSSFPSQLVHH134 pKa = 5.79TKK136 pKa = 10.12ARR138 pKa = 11.84RR139 pKa = 11.84TIGRR143 pKa = 11.84SRR145 pKa = 11.84AGFFLRR151 pKa = 11.84TILHH155 pKa = 5.89TLRR158 pKa = 11.84TVIGSS163 pKa = 3.48

Molecular weight: 18.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12852

0

12852

5104399

49

4988

397.2

43.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.603 ± 0.019

1.303 ± 0.009

5.447 ± 0.016

5.715 ± 0.02

3.719 ± 0.015

6.785 ± 0.03

2.641 ± 0.012

4.551 ± 0.015

4.382 ± 0.02

9.241 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.148 ± 0.008

3.349 ± 0.011

6.48 ± 0.025

3.756 ± 0.014

6.145 ± 0.02

9.16 ± 0.026

6.177 ± 0.012

6.369 ± 0.014

1.403 ± 0.008

2.625 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski