Blueberry shock virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Ilarvirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T1WFS1|T1WFS1_9BROM RNA-directed RNA polymerase 2a OS=Blueberry shock virus OX=747056 PE=4 SV=1
MM1 pKa = 7.68AFLSSMFPSDD11 pKa = 4.17SFLDD15 pKa = 4.0RR16 pKa = 11.84DD17 pKa = 3.75WEE19 pKa = 4.53VPLLLDD25 pKa = 3.5EE26 pKa = 4.74RR27 pKa = 11.84PRR29 pKa = 11.84TEE31 pKa = 3.88VEE33 pKa = 3.92QVSNEE38 pKa = 4.06EE39 pKa = 3.79VDD41 pKa = 3.78TNALFLKK48 pKa = 10.43SYY50 pKa = 10.87LLTLFLSPKK59 pKa = 9.74FKK61 pKa = 11.17YY62 pKa = 10.14IFEE65 pKa = 5.1DD66 pKa = 4.0FLGTEE71 pKa = 4.58FACYY75 pKa = 9.86VKK77 pKa = 10.34PRR79 pKa = 11.84WAIVFGCEE87 pKa = 3.14VDD89 pKa = 4.05YY90 pKa = 10.99EE91 pKa = 4.35VHH93 pKa = 6.4EE94 pKa = 4.46LVNDD98 pKa = 3.92EE99 pKa = 5.22LPLHH103 pKa = 6.88LDD105 pKa = 3.31TYY107 pKa = 9.87EE108 pKa = 3.94PWEE111 pKa = 4.34GVDD114 pKa = 6.1DD115 pKa = 4.68EE116 pKa = 4.97PCLGIDD122 pKa = 4.99DD123 pKa = 5.48LLDD126 pKa = 4.28RR127 pKa = 11.84DD128 pKa = 3.91ASPVEE133 pKa = 3.89RR134 pKa = 11.84DD135 pKa = 3.5DD136 pKa = 5.57VLFCLEE142 pKa = 4.92DD143 pKa = 3.81EE144 pKa = 4.63SDD146 pKa = 3.71GTTSLVLPTQTGIDD160 pKa = 4.17DD161 pKa = 5.36DD162 pKa = 4.66VDD164 pKa = 4.25SDD166 pKa = 3.77TSLKK170 pKa = 10.59VFEE173 pKa = 4.38STEE176 pKa = 3.92DD177 pKa = 3.78LEE179 pKa = 5.23DD180 pKa = 3.48VKK182 pKa = 10.53PTLKK186 pKa = 10.89SSDD189 pKa = 3.38LVLRR193 pKa = 11.84TINDD197 pKa = 3.25VDD199 pKa = 3.81TVIPVVEE206 pKa = 4.25KK207 pKa = 10.57FDD209 pKa = 3.91TNRR212 pKa = 11.84LHH214 pKa = 6.88LVKK217 pKa = 10.14EE218 pKa = 4.04VMMDD222 pKa = 3.5GFEE225 pKa = 4.43SLLIAHH231 pKa = 6.33EE232 pKa = 5.2RR233 pKa = 11.84ILSRR237 pKa = 11.84NDD239 pKa = 2.97WASLAMYY246 pKa = 9.12CPDD249 pKa = 4.33MEE251 pKa = 5.74IPTCGDD257 pKa = 3.35LLEE260 pKa = 5.48IPVKK264 pKa = 9.95HH265 pKa = 6.24TDD267 pKa = 2.83IEE269 pKa = 4.72FIQMAVDD276 pKa = 3.45EE277 pKa = 5.08LLPGVCDD284 pKa = 4.24LNDD287 pKa = 3.6SFFQEE292 pKa = 4.49LVEE295 pKa = 4.45TGDD298 pKa = 3.85IALEE302 pKa = 3.9LDD304 pKa = 3.15KK305 pKa = 11.57AKK307 pKa = 10.18IDD309 pKa = 3.2RR310 pKa = 11.84SVFNDD315 pKa = 2.81WSKK318 pKa = 11.44SKK320 pKa = 10.63KK321 pKa = 10.12CISSRR326 pKa = 11.84LNTGNISKK334 pKa = 10.28RR335 pKa = 11.84VPTFRR340 pKa = 11.84EE341 pKa = 3.27AALAIKK347 pKa = 9.8KK348 pKa = 10.33RR349 pKa = 11.84NLNVPDD355 pKa = 3.92LQQVFFEE362 pKa = 4.65DD363 pKa = 3.63EE364 pKa = 3.84EE365 pKa = 4.12ARR367 pKa = 11.84RR368 pKa = 11.84IANRR372 pKa = 11.84FINTVLDD379 pKa = 4.21PNKK382 pKa = 9.9LAQFPGYY389 pKa = 9.69ISEE392 pKa = 4.62GEE394 pKa = 3.3IGYY397 pKa = 9.03YY398 pKa = 10.14NKK400 pKa = 10.62YY401 pKa = 9.06LTGKK405 pKa = 10.48AIDD408 pKa = 4.01PDD410 pKa = 4.08MFVDD414 pKa = 4.38PCALVSMDD422 pKa = 4.87KK423 pKa = 9.87YY424 pKa = 10.83RR425 pKa = 11.84HH426 pKa = 5.56MIKK429 pKa = 7.8TTLKK433 pKa = 10.61PMEE436 pKa = 4.78DD437 pKa = 3.33CSGLFEE443 pKa = 5.02RR444 pKa = 11.84PLPATITYY452 pKa = 9.28HH453 pKa = 7.58DD454 pKa = 4.21KK455 pKa = 11.19GKK457 pKa = 10.97VMSTSPIFLMMANRR471 pKa = 11.84LMLCLNDD478 pKa = 4.85KK479 pKa = 10.77ISIPSGKK486 pKa = 9.13YY487 pKa = 8.54HH488 pKa = 6.33QLFSVDD494 pKa = 3.26PFAFEE499 pKa = 3.88TTKK502 pKa = 10.48EE503 pKa = 4.0FKK505 pKa = 10.61EE506 pKa = 3.73IDD508 pKa = 3.38FSKK511 pKa = 10.51FDD513 pKa = 3.39KK514 pKa = 10.68SQQRR518 pKa = 11.84LHH520 pKa = 7.41HH521 pKa = 7.01LIQFHH526 pKa = 6.82IFTALGASSEE536 pKa = 4.1FLQMWFGSHH545 pKa = 5.69EE546 pKa = 4.27LSHH549 pKa = 8.5ISDD552 pKa = 4.63GPCGIGFSVNYY563 pKa = 8.16QRR565 pKa = 11.84RR566 pKa = 11.84TGDD569 pKa = 2.69ACTYY573 pKa = 10.06LGNTIITLSALAYY586 pKa = 9.8MYY588 pKa = 10.97DD589 pKa = 3.7LLDD592 pKa = 4.26PNVTFVIASGDD603 pKa = 3.52DD604 pKa = 3.56SLIGSIKK611 pKa = 9.66PLNRR615 pKa = 11.84EE616 pKa = 3.99DD617 pKa = 3.82EE618 pKa = 4.69YY619 pKa = 11.79KK620 pKa = 9.79FTTLFNFEE628 pKa = 4.46AKK630 pKa = 9.98FPHH633 pKa = 6.26NMPFVCSKK641 pKa = 10.38FLILAPTNDD650 pKa = 3.44GGKK653 pKa = 10.0KK654 pKa = 9.37VVAVPNALKK663 pKa = 10.82LFIKK667 pKa = 10.63LGVKK671 pKa = 10.06DD672 pKa = 3.83LSPYY676 pKa = 9.92VFNEE680 pKa = 4.58WYY682 pKa = 10.58SSWLDD687 pKa = 3.86LIWYY691 pKa = 8.15FDD693 pKa = 3.65NYY695 pKa = 10.63HH696 pKa = 5.86VVSTMRR702 pKa = 11.84DD703 pKa = 3.87YY704 pKa = 11.5LSHH707 pKa = 6.83RR708 pKa = 11.84YY709 pKa = 9.32KK710 pKa = 10.82RR711 pKa = 11.84GTTFYY716 pKa = 11.03QEE718 pKa = 4.21GGMLALRR725 pKa = 11.84SVFSSKK731 pKa = 9.31TKK733 pKa = 9.91CLKK736 pKa = 10.61VLFGLEE742 pKa = 4.06PKK744 pKa = 10.41DD745 pKa = 3.64IEE747 pKa = 4.19EE748 pKa = 4.51PKK750 pKa = 9.77PKK752 pKa = 9.93KK753 pKa = 10.54VSFNQIEE760 pKa = 4.06RR761 pKa = 11.84LNFRR765 pKa = 11.84EE766 pKa = 4.53KK767 pKa = 10.74EE768 pKa = 4.06NKK770 pKa = 9.36VPNKK774 pKa = 10.12DD775 pKa = 3.05RR776 pKa = 11.84SKK778 pKa = 10.91RR779 pKa = 11.84PNKK782 pKa = 9.54SSRR785 pKa = 11.84MNSDD789 pKa = 3.31LKK791 pKa = 11.33

Molecular weight: 90.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T1WEY7|T1WEY7_9BROM ATP-dependent helicase OS=Blueberry shock virus OX=747056 PE=3 SV=1
MM1 pKa = 6.99VCKK4 pKa = 10.52FCNHH8 pKa = 4.85THH10 pKa = 7.29AGGCGQCKK18 pKa = 9.46KK19 pKa = 10.65CHH21 pKa = 4.81GTKK24 pKa = 10.41AAGPSVKK31 pKa = 10.67AQDD34 pKa = 3.36RR35 pKa = 11.84AKK37 pKa = 10.85NNPNKK42 pKa = 10.23GSPSGTKK49 pKa = 10.18SPGAKK54 pKa = 9.65GAPKK58 pKa = 10.23DD59 pKa = 3.54KK60 pKa = 10.48QVQKK64 pKa = 9.75RR65 pKa = 11.84TDD67 pKa = 3.0WTVIGPNVQPVNHH80 pKa = 7.16PNGFTLRR87 pKa = 11.84SCRR90 pKa = 11.84DD91 pKa = 3.23VQATVAGKK99 pKa = 9.64FLHH102 pKa = 6.99INFKK106 pKa = 9.4TAFPQLLNQEE116 pKa = 4.11LKK118 pKa = 9.89IYY120 pKa = 10.25SFAVRR125 pKa = 11.84CSTSIGNGWVGLVRR139 pKa = 11.84GFNPSSPTGPAVLTRR154 pKa = 11.84KK155 pKa = 10.04GFLKK159 pKa = 10.61DD160 pKa = 4.09QARR163 pKa = 11.84GWQWLAPSDD172 pKa = 3.99LEE174 pKa = 3.81YY175 pKa = 11.29DD176 pKa = 3.69KK177 pKa = 11.28FSEE180 pKa = 4.43EE181 pKa = 4.18YY182 pKa = 10.47EE183 pKa = 4.07LVFEE187 pKa = 5.03FKK189 pKa = 10.22SDD191 pKa = 3.7YY192 pKa = 10.62PIGVVMTRR200 pKa = 11.84DD201 pKa = 3.7LYY203 pKa = 11.56VVTSSLPRR211 pKa = 11.84VRR213 pKa = 11.84IPDD216 pKa = 3.72DD217 pKa = 3.7LLFVDD222 pKa = 5.08EE223 pKa = 5.82DD224 pKa = 3.89LLEE227 pKa = 4.16II228 pKa = 5.03

Molecular weight: 25.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2359

228

1045

589.8

66.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.189 ± 0.972

1.95 ± 0.212

6.825 ± 0.783

6.783 ± 0.506

5.002 ± 0.67

5.256 ± 0.659

2.331 ± 0.234

5.002 ± 0.308

7.079 ± 0.209

9.496 ± 0.648

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.374 ± 0.412

4.324 ± 0.078

4.409 ± 0.811

2.671 ± 0.436

5.511 ± 0.508

7.418 ± 0.172

6.062 ± 0.386

7.206 ± 0.346

1.145 ± 0.097

2.967 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski