Amino acid dipepetide frequency for Chloroflexi bacterium (strain G233)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.663AlaAla: 22.663 ± 0.283	1.231AlaCys: 1.231 ± 0.04	6.247AlaAsp: 6.247 ± 0.098	9.076AlaGlu: 9.076 ± 0.154	4.41AlaPhe: 4.41 ± 0.09	12.979AlaGly: 12.979 ± 0.134	2.165AlaHis: 2.165 ± 0.065	6.408AlaIle: 6.408 ± 0.093	2.539AlaLys: 2.539 ± 0.067	13.783AlaLeu: 13.783 ± 0.148	2.984AlaMet: 2.984 ± 0.069	2.584AlaAsn: 2.584 ± 0.061	6.625AlaPro: 6.625 ± 0.136	3.258AlaGln: 3.258 ± 0.072	11.233AlaArg: 11.233 ± 0.132	5.641AlaSer: 5.641 ± 0.093	6.148AlaThr: 6.148 ± 0.11	10.627AlaVal: 10.627 ± 0.136	2.246AlaTrp: 2.246 ± 0.056	2.609AlaTyr: 2.609 ± 0.061	0.0AlaXaa: 0.0 ± 0.0
Cys	0.961CysAla: 0.961 ± 0.036	0.101CysCys: 0.101 ± 0.012	0.516CysAsp: 0.516 ± 0.029	0.52CysGlu: 0.52 ± 0.026	0.251CysPhe: 0.251 ± 0.017	1.025CysGly: 1.025 ± 0.037	0.208CysHis: 0.208 ± 0.016	0.422CysIle: 0.422 ± 0.023	0.137CysLys: 0.137 ± 0.013	0.695CysLeu: 0.695 ± 0.032	0.135CysMet: 0.135 ± 0.012	0.214CysAsn: 0.214 ± 0.015	0.564CysPro: 0.564 ± 0.031	0.223CysGln: 0.223 ± 0.018	0.712CysArg: 0.712 ± 0.029	0.424CysSer: 0.424 ± 0.024	0.45CysThr: 0.45 ± 0.029	0.537CysVal: 0.537 ± 0.024	0.119CysTrp: 0.119 ± 0.011	0.245CysTyr: 0.245 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	6.426AspAla: 6.426 ± 0.095	0.42AspCys: 0.42 ± 0.025	2.926AspAsp: 2.926 ± 0.073	3.555AspGlu: 3.555 ± 0.069	1.788AspPhe: 1.788 ± 0.047	4.707AspGly: 4.707 ± 0.084	0.963AspHis: 0.963 ± 0.037	2.67AspIle: 2.67 ± 0.062	0.624AspLys: 0.624 ± 0.023	5.808AspLeu: 5.808 ± 0.083	0.821AspMet: 0.821 ± 0.033	0.778AspAsn: 0.778 ± 0.033	4.28AspPro: 4.28 ± 0.09	1.225AspGln: 1.225 ± 0.043	4.435AspArg: 4.435 ± 0.077	1.601AspSer: 1.601 ± 0.041	2.214AspThr: 2.214 ± 0.045	4.076AspVal: 4.076 ± 0.074	0.794AspTrp: 0.794 ± 0.027	1.311AspTyr: 1.311 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	9.381GluAla: 9.381 ± 0.126	0.431GluCys: 0.431 ± 0.022	2.681GluAsp: 2.681 ± 0.055	4.455GluGlu: 4.455 ± 0.109	2.149GluPhe: 2.149 ± 0.054	5.13GluGly: 5.13 ± 0.079	1.496GluHis: 1.496 ± 0.042	2.794GluIle: 2.794 ± 0.067	1.687GluLys: 1.687 ± 0.047	7.347GluLeu: 7.347 ± 0.109	1.291GluMet: 1.291 ± 0.045	1.26GluAsn: 1.26 ± 0.041	3.995GluPro: 3.995 ± 0.081	2.384GluGln: 2.384 ± 0.059	7.072GluArg: 7.072 ± 0.112	2.276GluSer: 2.276 ± 0.055	3.335GluThr: 3.335 ± 0.059	5.033GluVal: 5.033 ± 0.086	0.87GluTrp: 0.87 ± 0.036	1.439GluTyr: 1.439 ± 0.047	0.0GluXaa: 0.0 ± 0.0
Phe	4.406PheAla: 4.406 ± 0.083	0.348PheCys: 0.348 ± 0.021	2.311PheAsp: 2.311 ± 0.053	2.566PheGlu: 2.566 ± 0.056	1.16PhePhe: 1.16 ± 0.041	3.519PheGly: 3.519 ± 0.069	0.617PheHis: 0.617 ± 0.031	1.55PheIle: 1.55 ± 0.044	0.661PheLys: 0.661 ± 0.033	3.116PheLeu: 3.116 ± 0.074	0.505PheMet: 0.505 ± 0.024	0.797PheAsn: 0.797 ± 0.032	1.626PhePro: 1.626 ± 0.048	0.813PheGln: 0.813 ± 0.032	2.652PheArg: 2.652 ± 0.053	1.562PheSer: 1.562 ± 0.047	1.956PheThr: 1.956 ± 0.053	2.635PheVal: 2.635 ± 0.05	0.486PheTrp: 0.486 ± 0.023	0.759PheTyr: 0.759 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	10.342GlyAla: 10.342 ± 0.136	0.815GlyCys: 0.815 ± 0.036	4.366GlyAsp: 4.366 ± 0.079	5.484GlyGlu: 5.484 ± 0.084	3.239GlyPhe: 3.239 ± 0.069	8.072GlyGly: 8.072 ± 0.136	1.775GlyHis: 1.775 ± 0.051	4.717GlyIle: 4.717 ± 0.076	2.233GlyLys: 2.233 ± 0.055	8.918GlyLeu: 8.918 ± 0.116	2.309GlyMet: 2.309 ± 0.053	1.83GlyAsn: 1.83 ± 0.057	4.889GlyPro: 4.889 ± 0.082	2.92GlyGln: 2.92 ± 0.067	7.731GlyArg: 7.731 ± 0.099	4.403GlySer: 4.403 ± 0.08	5.264GlyThr: 5.264 ± 0.105	6.591GlyVal: 6.591 ± 0.096	1.706GlyTrp: 1.706 ± 0.052	2.422GlyTyr: 2.422 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	2.121HisAla: 2.121 ± 0.051	0.208HisCys: 0.208 ± 0.016	1.06HisAsp: 1.06 ± 0.039	1.06HisGlu: 1.06 ± 0.036	0.605HisPhe: 0.605 ± 0.031	1.823HisGly: 1.823 ± 0.058	0.531HisHis: 0.531 ± 0.036	0.918HisIle: 0.918 ± 0.038	0.242HisLys: 0.242 ± 0.017	2.029HisLeu: 2.029 ± 0.049	0.356HisMet: 0.356 ± 0.021	0.404HisAsn: 0.404 ± 0.022	1.528HisPro: 1.528 ± 0.046	0.555HisGln: 0.555 ± 0.027	1.621HisArg: 1.621 ± 0.045	0.675HisSer: 0.675 ± 0.031	0.901HisThr: 0.901 ± 0.037	1.314HisVal: 1.314 ± 0.047	0.301HisTrp: 0.301 ± 0.02	0.49HisTyr: 0.49 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	6.606IleAla: 6.606 ± 0.095	0.42IleCys: 0.42 ± 0.021	3.13IleAsp: 3.13 ± 0.067	3.428IleGlu: 3.428 ± 0.068	1.287IlePhe: 1.287 ± 0.039	4.214IleGly: 4.214 ± 0.077	0.855IleHis: 0.855 ± 0.033	2.074IleIle: 2.074 ± 0.062	0.887IleLys: 0.887 ± 0.039	4.001IleLeu: 4.001 ± 0.072	0.587IleMet: 0.587 ± 0.031	1.032IleAsn: 1.032 ± 0.042	2.56IlePro: 2.56 ± 0.056	1.22IleGln: 1.22 ± 0.037	3.577IleArg: 3.577 ± 0.068	2.012IleSer: 2.012 ± 0.049	2.505IleThr: 2.505 ± 0.063	3.753IleVal: 3.753 ± 0.064	0.439IleTrp: 0.439 ± 0.023	0.923IleTyr: 0.923 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	2.369LysAla: 2.369 ± 0.062	0.137LysCys: 0.137 ± 0.014	1.023LysAsp: 1.023 ± 0.037	1.474LysGlu: 1.474 ± 0.045	0.615LysPhe: 0.615 ± 0.027	1.66LysGly: 1.66 ± 0.052	0.389LysHis: 0.389 ± 0.022	0.911LysIle: 0.911 ± 0.041	0.748LysLys: 0.748 ± 0.034	2.068LysLeu: 2.068 ± 0.056	0.45LysMet: 0.45 ± 0.026	0.54LysAsn: 0.54 ± 0.027	1.314LysPro: 1.314 ± 0.043	0.705LysGln: 0.705 ± 0.031	1.629LysArg: 1.629 ± 0.048	0.895LysSer: 0.895 ± 0.033	1.124LysThr: 1.124 ± 0.036	1.818LysVal: 1.818 ± 0.056	0.256LysTrp: 0.256 ± 0.018	0.563LysTyr: 0.563 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	15.832LeuAla: 15.832 ± 0.181	0.836LeuCys: 0.836 ± 0.032	5.665LeuAsp: 5.665 ± 0.085	7.243LeuGlu: 7.243 ± 0.117	3.187LeuPhe: 3.187 ± 0.072	8.966LeuGly: 8.966 ± 0.137	1.765LeuHis: 1.765 ± 0.052	3.703LeuIle: 3.703 ± 0.068	2.166LeuLys: 2.166 ± 0.054	10.323LeuLeu: 10.323 ± 0.142	1.582LeuMet: 1.582 ± 0.049	1.988LeuAsn: 1.988 ± 0.046	6.366LeuPro: 6.366 ± 0.094	2.936LeuGln: 2.936 ± 0.073	8.653LeuArg: 8.653 ± 0.115	4.279LeuSer: 4.279 ± 0.079	4.706LeuThr: 4.706 ± 0.074	8.243LeuVal: 8.243 ± 0.095	1.185LeuTrp: 1.185 ± 0.04	2.005LeuTyr: 2.005 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	2.854MetAla: 2.854 ± 0.072	0.142MetCys: 0.142 ± 0.013	0.872MetAsp: 0.872 ± 0.036	1.09MetGlu: 1.09 ± 0.044	0.579MetPhe: 0.579 ± 0.028	1.552MetGly: 1.552 ± 0.046	0.354MetHis: 0.354 ± 0.02	0.616MetIle: 0.616 ± 0.027	0.549MetLys: 0.549 ± 0.025	1.99MetLeu: 1.99 ± 0.048	0.354MetMet: 0.354 ± 0.02	0.537MetAsn: 0.537 ± 0.025	1.34MetPro: 1.34 ± 0.039	0.643MetGln: 0.643 ± 0.028	1.57MetArg: 1.57 ± 0.04	1.047MetSer: 1.047 ± 0.037	1.451MetThr: 1.451 ± 0.047	1.485MetVal: 1.485 ± 0.04	0.182MetTrp: 0.182 ± 0.016	0.343MetTyr: 0.343 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	2.475AsnAla: 2.475 ± 0.056	0.185AsnCys: 0.185 ± 0.017	1.071AsnAsp: 1.071 ± 0.039	1.174AsnGlu: 1.174 ± 0.036	0.764AsnPhe: 0.764 ± 0.029	1.959AsnGly: 1.959 ± 0.056	0.413AsnHis: 0.413 ± 0.025	1.101AsnIle: 1.101 ± 0.034	0.368AsnLys: 0.368 ± 0.023	2.214AsnLeu: 2.214 ± 0.052	0.401AsnMet: 0.401 ± 0.023	0.478AsnAsn: 0.478 ± 0.027	1.89AsnPro: 1.89 ± 0.049	0.553AsnGln: 0.553 ± 0.023	1.725AsnArg: 1.725 ± 0.048	0.756AsnSer: 0.756 ± 0.028	1.005AsnThr: 1.005 ± 0.036	1.665AsnVal: 1.665 ± 0.042	0.314AsnTrp: 0.314 ± 0.021	0.531AsnTyr: 0.531 ± 0.027	0.0AsnXaa: 0.0 ± 0.0
Pro	9.1ProAla: 9.1 ± 0.151	0.393ProCys: 0.393 ± 0.021	3.439ProAsp: 3.439 ± 0.064	4.698ProGlu: 4.698 ± 0.084	2.063ProPhe: 2.063 ± 0.045	7.348ProGly: 7.348 ± 0.107	1.05ProHis: 1.05 ± 0.037	2.102ProIle: 2.102 ± 0.052	1.156ProLys: 1.156 ± 0.043	5.342ProLeu: 5.342 ± 0.078	1.073ProMet: 1.073 ± 0.035	1.422ProAsn: 1.422 ± 0.042	4.461ProPro: 4.461 ± 0.112	1.421ProGln: 1.421 ± 0.035	4.319ProArg: 4.319 ± 0.068	2.744ProSer: 2.744 ± 0.069	3.084ProThr: 3.084 ± 0.11	4.984ProVal: 4.984 ± 0.089	0.913ProTrp: 0.913 ± 0.037	1.275ProTyr: 1.275 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	3.876GlnAla: 3.876 ± 0.074	0.185GlnCys: 0.185 ± 0.016	1.096GlnAsp: 1.096 ± 0.036	1.684GlnGlu: 1.684 ± 0.048	1.037GlnPhe: 1.037 ± 0.036	2.122GlnGly: 2.122 ± 0.051	0.58GlnHis: 0.58 ± 0.028	0.989GlnIle: 0.989 ± 0.042	0.722GlnLys: 0.722 ± 0.037	3.205GlnLeu: 3.205 ± 0.057	0.621GlnMet: 0.621 ± 0.032	0.588GlnAsn: 0.588 ± 0.025	1.964GlnPro: 1.964 ± 0.053	1.233GlnGln: 1.233 ± 0.045	2.633GlnArg: 2.633 ± 0.051	1.125GlnSer: 1.125 ± 0.044	1.206GlnThr: 1.206 ± 0.04	2.628GlnVal: 2.628 ± 0.055	0.433GlnTrp: 0.433 ± 0.025	0.694GlnTyr: 0.694 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	10.248ArgAla: 10.248 ± 0.132	0.686ArgCys: 0.686 ± 0.03	4.315ArgAsp: 4.315 ± 0.084	6.527ArgGlu: 6.527 ± 0.105	3.071ArgPhe: 3.071 ± 0.06	6.322ArgGly: 6.322 ± 0.092	1.704ArgHis: 1.704 ± 0.044	4.013ArgIle: 4.013 ± 0.079	1.875ArgLys: 1.875 ± 0.056	9.13ArgLeu: 9.13 ± 0.118	1.962ArgMet: 1.962 ± 0.049	1.799ArgAsn: 1.799 ± 0.044	4.986ArgPro: 4.986 ± 0.082	2.776ArgGln: 2.776 ± 0.055	7.806ArgArg: 7.806 ± 0.109	3.43ArgSer: 3.43 ± 0.062	3.882ArgThr: 3.882 ± 0.07	6.58ArgVal: 6.58 ± 0.103	1.532ArgTrp: 1.532 ± 0.05	2.179ArgTyr: 2.179 ± 0.052	0.0ArgXaa: 0.0 ± 0.0
Ser	5.022SerAla: 5.022 ± 0.101	0.354SerCys: 0.354 ± 0.021	2.124SerAsp: 2.124 ± 0.055	2.282SerGlu: 2.282 ± 0.054	1.575SerPhe: 1.575 ± 0.046	4.558SerGly: 4.558 ± 0.078	0.787SerHis: 0.787 ± 0.03	2.003SerIle: 2.003 ± 0.047	0.887SerLys: 0.887 ± 0.037	4.168SerLeu: 4.168 ± 0.086	0.961SerMet: 0.961 ± 0.034	0.895SerAsn: 0.895 ± 0.033	3.16SerPro: 3.16 ± 0.07	1.118SerGln: 1.118 ± 0.04	3.379SerArg: 3.379 ± 0.068	2.187SerSer: 2.187 ± 0.058	2.282SerThr: 2.282 ± 0.061	3.197SerVal: 3.197 ± 0.066	0.701SerTrp: 0.701 ± 0.033	1.03SerTyr: 1.03 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	6.371ThrAla: 6.371 ± 0.108	0.44ThrCys: 0.44 ± 0.025	2.315ThrAsp: 2.315 ± 0.06	2.489ThrGlu: 2.489 ± 0.053	1.96ThrPhe: 1.96 ± 0.057	5.185ThrGly: 5.185 ± 0.084	0.851ThrHis: 0.851 ± 0.034	2.74ThrIle: 2.74 ± 0.067	0.987ThrLys: 0.987 ± 0.038	5.002ThrLeu: 5.002 ± 0.093	0.931ThrMet: 0.931 ± 0.033	1.117ThrAsn: 1.117 ± 0.041	4.01ThrPro: 4.01 ± 0.133	1.155ThrGln: 1.155 ± 0.04	3.571ThrArg: 3.571 ± 0.064	2.391ThrSer: 2.391 ± 0.06	2.669ThrThr: 2.669 ± 0.061	4.612ThrVal: 4.612 ± 0.086	0.893ThrTrp: 0.893 ± 0.034	1.197ThrTyr: 1.197 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	10.439ValAla: 10.439 ± 0.122	0.688ValCys: 0.688 ± 0.035	4.196ValAsp: 4.196 ± 0.066	5.288ValGlu: 5.288 ± 0.088	2.808ValPhe: 2.808 ± 0.065	5.759ValGly: 5.759 ± 0.084	1.404ValHis: 1.404 ± 0.045	4.378ValIle: 4.378 ± 0.078	1.532ValLys: 1.532 ± 0.05	8.122ValLeu: 8.122 ± 0.109	1.558ValMet: 1.558 ± 0.052	1.87ValAsn: 1.87 ± 0.057	4.828ValPro: 4.828 ± 0.071	2.132ValGln: 2.132 ± 0.046	6.737ValArg: 6.737 ± 0.104	3.601ValSer: 3.601 ± 0.073	4.768ValThr: 4.768 ± 0.108	7.352ValVal: 7.352 ± 0.127	0.682ValTrp: 0.682 ± 0.026	1.807ValTyr: 1.807 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	1.539TrpAla: 1.539 ± 0.047	0.176TrpCys: 0.176 ± 0.015	0.74TrpAsp: 0.74 ± 0.029	1.011TrpGlu: 1.011 ± 0.041	0.614TrpPhe: 0.614 ± 0.031	1.04TrpGly: 1.04 ± 0.036	0.307TrpHis: 0.307 ± 0.02	0.416TrpIle: 0.416 ± 0.023	0.374TrpLys: 0.374 ± 0.022	1.795TrpLeu: 1.795 ± 0.054	0.328TrpMet: 0.328 ± 0.019	0.411TrpAsn: 0.411 ± 0.025	0.718TrpPro: 0.718 ± 0.032	0.59TrpGln: 0.59 ± 0.026	1.379TrpArg: 1.379 ± 0.045	0.639TrpSer: 0.639 ± 0.029	0.759TrpThr: 0.759 ± 0.03	1.162TrpVal: 1.162 ± 0.038	0.337TrpTrp: 0.337 ± 0.025	0.403TrpTyr: 0.403 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.424TyrAla: 2.424 ± 0.053	0.265TyrCys: 0.265 ± 0.023	1.332TyrAsp: 1.332 ± 0.043	1.398TyrGlu: 1.398 ± 0.043	0.883TyrPhe: 0.883 ± 0.032	2.085TyrGly: 2.085 ± 0.053	0.531TyrHis: 0.531 ± 0.025	0.984TyrIle: 0.984 ± 0.037	0.316TyrLys: 0.316 ± 0.021	2.574TyrLeu: 2.574 ± 0.054	0.337TyrMet: 0.337 ± 0.019	0.497TyrAsn: 0.497 ± 0.032	1.286TyrPro: 1.286 ± 0.043	0.763TyrGln: 0.763 ± 0.025	2.386TyrArg: 2.386 ± 0.059	0.983TyrSer: 0.983 ± 0.034	1.176TyrThr: 1.176 ± 0.037	1.658TyrVal: 1.658 ± 0.042	0.348TyrTrp: 0.348 ± 0.021	0.624TyrTyr: 0.624 ± 0.029	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2626 proteins (831118 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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