Myotis lucifugus (Little brown bat)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Chiroptera; Microchiroptera;

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20651 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1Q1D6|G1Q1D6_MYOLU ABC transporter domain-containing protein OS=Myotis lucifugus OX=59463 PE=4 SV=1
MM1 pKa = 7.31EE2 pKa = 5.18AAILLPLALGLLLLPLLAVLLMALCVRR29 pKa = 11.84CRR31 pKa = 11.84EE32 pKa = 4.17LPDD35 pKa = 3.27TYY37 pKa = 10.45TYY39 pKa = 11.37DD40 pKa = 3.47SAVPDD45 pKa = 3.81SLPRR49 pKa = 11.84SIVIKK54 pKa = 10.55RR55 pKa = 11.84PATLAPWPQATVTSCPPLSPPDD77 pKa = 3.97LLPIPRR83 pKa = 11.84SPQPPGGSHH92 pKa = 6.76RR93 pKa = 11.84MPSARR98 pKa = 11.84QDD100 pKa = 3.26SDD102 pKa = 3.27GTNSVASYY110 pKa = 10.58EE111 pKa = 4.2NEE113 pKa = 3.76EE114 pKa = 4.21PACEE118 pKa = 4.53DD119 pKa = 3.53EE120 pKa = 5.76DD121 pKa = 5.71DD122 pKa = 4.9EE123 pKa = 5.49EE124 pKa = 4.43EE125 pKa = 4.81DD126 pKa = 3.78YY127 pKa = 11.57HH128 pKa = 8.34NEE130 pKa = 4.04GYY132 pKa = 10.82LVVLPDD138 pKa = 4.13TVPATATATCAAVPAAAAPSSPGPRR163 pKa = 11.84DD164 pKa = 3.13SAFSMEE170 pKa = 4.23SGEE173 pKa = 4.99DD174 pKa = 3.63YY175 pKa = 11.66VNVPEE180 pKa = 4.68SEE182 pKa = 4.29EE183 pKa = 4.08SADD186 pKa = 3.61ASLVGAGKK194 pKa = 10.16YY195 pKa = 9.46VNVSQEE201 pKa = 3.83LQPTVRR207 pKa = 11.84TEE209 pKa = 3.87PATLASQEE217 pKa = 4.05AEE219 pKa = 4.08EE220 pKa = 4.52EE221 pKa = 4.35EE222 pKa = 4.8EE223 pKa = 4.45EE224 pKa = 4.84GAPDD228 pKa = 3.86YY229 pKa = 11.54EE230 pKa = 4.6NLQEE234 pKa = 4.11LQQ236 pKa = 3.33

Molecular weight: 25.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1Q7W3|G1Q7W3_MYOLU Uncharacterized protein OS=Myotis lucifugus OX=59463 GN=WFDC11 PE=4 SV=1
YYY2 pKa = 9.28HH3 pKa = 5.54YY4 pKa = 9.4HH5 pKa = 5.39HH6 pKa = 6.98HH7 pKa = 5.96MRR9 pKa = 11.84RR10 pKa = 11.84KKK12 pKa = 7.84RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84GKKK23 pKa = 8.85KK24 pKa = 8.0RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84GRR31 pKa = 11.84RR32 pKa = 11.84KKK34 pKa = 8.41RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84

Molecular weight: 5.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19655

996

20651

10579164

10

34984

512.3

57.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.959 ± 0.018

2.251 ± 0.015

4.805 ± 0.012

7.014 ± 0.021

3.71 ± 0.015

6.55 ± 0.023

2.588 ± 0.01

4.375 ± 0.015

5.729 ± 0.02

10.051 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.126 ± 0.009

3.6 ± 0.015

6.278 ± 0.029

4.779 ± 0.017

5.672 ± 0.017

8.241 ± 0.02

5.256 ± 0.013

6.084 ± 0.016

1.238 ± 0.007

2.69 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski