Amino acid dipepetide frequency for Mycobacterium phage Sbash

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.198AlaAla: 16.198 ± 1.13
1.106AlaCys: 1.106 ± 0.289
7.905AlaAsp: 7.905 ± 0.635
8.126AlaGlu: 8.126 ± 0.942
2.488AlaPhe: 2.488 ± 0.448
9.896AlaGly: 9.896 ± 1.06
2.598AlaHis: 2.598 ± 0.369
5.86AlaIle: 5.86 ± 0.559
3.925AlaLys: 3.925 ± 0.52
9.619AlaLeu: 9.619 ± 0.773
2.819AlaMet: 2.819 ± 0.445
3.649AlaAsn: 3.649 ± 0.478
6.413AlaPro: 6.413 ± 0.784
4.146AlaGln: 4.146 ± 0.743
7.74AlaArg: 7.74 ± 0.655
5.528AlaSer: 5.528 ± 0.555
6.689AlaThr: 6.689 ± 0.556
6.8AlaVal: 6.8 ± 0.638
1.88AlaTrp: 1.88 ± 0.386
3.096AlaTyr: 3.096 ± 0.373
0.0AlaXaa: 0.0 ± 0.0
Cys
1.05CysAla: 1.05 ± 0.203
0.276CysCys: 0.276 ± 0.157
0.94CysAsp: 0.94 ± 0.242
0.608CysGlu: 0.608 ± 0.184
0.055CysPhe: 0.055 ± 0.057
1.437CysGly: 1.437 ± 0.32
0.332CysHis: 0.332 ± 0.153
0.442CysIle: 0.442 ± 0.142
0.221CysLys: 0.221 ± 0.107
0.553CysLeu: 0.553 ± 0.173
0.332CysMet: 0.332 ± 0.126
0.276CysAsn: 0.276 ± 0.144
1.05CysPro: 1.05 ± 0.276
0.553CysGln: 0.553 ± 0.187
0.94CysArg: 0.94 ± 0.236
0.442CysSer: 0.442 ± 0.131
0.719CysThr: 0.719 ± 0.234
0.442CysVal: 0.442 ± 0.158
0.276CysTrp: 0.276 ± 0.143
0.442CysTyr: 0.442 ± 0.157
0.0CysXaa: 0.0 ± 0.0
Asp
7.353AspAla: 7.353 ± 0.833
0.829AspCys: 0.829 ± 0.216
6.081AspAsp: 6.081 ± 0.733
5.307AspGlu: 5.307 ± 0.5
1.216AspPhe: 1.216 ± 0.223
7.518AspGly: 7.518 ± 0.698
0.995AspHis: 0.995 ± 0.251
1.99AspIle: 1.99 ± 0.283
1.161AspLys: 1.161 ± 0.231
7.684AspLeu: 7.684 ± 0.661
1.216AspMet: 1.216 ± 0.233
1.603AspAsn: 1.603 ± 0.32
3.814AspPro: 3.814 ± 0.414
2.267AspGln: 2.267 ± 0.346
3.759AspArg: 3.759 ± 0.44
2.764AspSer: 2.764 ± 0.363
3.704AspThr: 3.704 ± 0.419
4.146AspVal: 4.146 ± 0.508
1.437AspTrp: 1.437 ± 0.291
1.493AspTyr: 1.493 ± 0.249
0.0AspXaa: 0.0 ± 0.0
Glu
7.187GluAla: 7.187 ± 0.746
0.663GluCys: 0.663 ± 0.182
2.543GluAsp: 2.543 ± 0.423
2.875GluGlu: 2.875 ± 0.567
2.432GluPhe: 2.432 ± 0.431
3.704GluGly: 3.704 ± 0.474
1.658GluHis: 1.658 ± 0.336
1.769GluIle: 1.769 ± 0.424
2.322GluLys: 2.322 ± 0.318
6.579GluLeu: 6.579 ± 0.807
1.106GluMet: 1.106 ± 0.269
1.824GluAsn: 1.824 ± 0.265
3.925GluPro: 3.925 ± 0.484
1.935GluGln: 1.935 ± 0.387
4.423GluArg: 4.423 ± 0.438
2.488GluSer: 2.488 ± 0.542
3.925GluThr: 3.925 ± 0.488
4.81GluVal: 4.81 ± 0.517
1.382GluTrp: 1.382 ± 0.277
1.935GluTyr: 1.935 ± 0.298
0.0GluXaa: 0.0 ± 0.0
Phe
2.93PheAla: 2.93 ± 0.492
0.332PheCys: 0.332 ± 0.122
1.714PheAsp: 1.714 ± 0.353
1.437PheGlu: 1.437 ± 0.229
0.442PhePhe: 0.442 ± 0.134
3.096PheGly: 3.096 ± 0.456
0.332PheHis: 0.332 ± 0.13
1.714PheIle: 1.714 ± 0.326
1.216PheLys: 1.216 ± 0.224
2.045PheLeu: 2.045 ± 0.272
0.553PheMet: 0.553 ± 0.194
0.94PheAsn: 0.94 ± 0.192
0.663PhePro: 0.663 ± 0.174
0.885PheGln: 0.885 ± 0.23
2.101PheArg: 2.101 ± 0.291
1.216PheSer: 1.216 ± 0.303
1.824PheThr: 1.824 ± 0.306
1.88PheVal: 1.88 ± 0.319
0.608PheTrp: 0.608 ± 0.177
0.553PheTyr: 0.553 ± 0.184
0.0PheXaa: 0.0 ± 0.0
Gly
8.569GlyAla: 8.569 ± 1.089
1.106GlyCys: 1.106 ± 0.29
4.312GlyAsp: 4.312 ± 0.458
4.257GlyGlu: 4.257 ± 0.559
2.819GlyPhe: 2.819 ± 0.514
10.669GlyGly: 10.669 ± 2.107
1.603GlyHis: 1.603 ± 0.33
4.091GlyIle: 4.091 ± 0.592
3.262GlyLys: 3.262 ± 0.426
7.187GlyLeu: 7.187 ± 0.725
1.935GlyMet: 1.935 ± 0.331
2.432GlyAsn: 2.432 ± 0.439
5.086GlyPro: 5.086 ± 0.651
3.427GlyGln: 3.427 ± 0.72
6.413GlyArg: 6.413 ± 0.54
5.086GlySer: 5.086 ± 0.679
6.081GlyThr: 6.081 ± 0.737
5.86GlyVal: 5.86 ± 0.649
2.377GlyTrp: 2.377 ± 0.395
2.377GlyTyr: 2.377 ± 0.303
0.0GlyXaa: 0.0 ± 0.0
His
2.598HisAla: 2.598 ± 0.567
0.332HisCys: 0.332 ± 0.134
1.327HisAsp: 1.327 ± 0.237
0.829HisGlu: 0.829 ± 0.198
0.498HisPhe: 0.498 ± 0.177
1.327HisGly: 1.327 ± 0.276
0.553HisHis: 0.553 ± 0.186
0.774HisIle: 0.774 ± 0.237
0.608HisLys: 0.608 ± 0.2
1.88HisLeu: 1.88 ± 0.327
0.387HisMet: 0.387 ± 0.124
0.774HisAsn: 0.774 ± 0.199
1.935HisPro: 1.935 ± 0.387
0.885HisGln: 0.885 ± 0.231
1.603HisArg: 1.603 ± 0.419
0.774HisSer: 0.774 ± 0.225
1.327HisThr: 1.327 ± 0.254
1.714HisVal: 1.714 ± 0.328
0.276HisTrp: 0.276 ± 0.105
0.774HisTyr: 0.774 ± 0.213
0.0HisXaa: 0.0 ± 0.0
Ile
5.528IleAla: 5.528 ± 0.643
0.332IleCys: 0.332 ± 0.139
3.427IleAsp: 3.427 ± 0.459
3.206IleGlu: 3.206 ± 0.466
1.106IlePhe: 1.106 ± 0.259
4.257IleGly: 4.257 ± 0.511
0.663IleHis: 0.663 ± 0.212
1.327IleIle: 1.327 ± 0.306
1.271IleLys: 1.271 ± 0.256
2.377IleLeu: 2.377 ± 0.342
0.332IleMet: 0.332 ± 0.114
1.382IleAsn: 1.382 ± 0.255
2.93IlePro: 2.93 ± 0.42
1.271IleGln: 1.271 ± 0.223
2.598IleArg: 2.598 ± 0.335
2.211IleSer: 2.211 ± 0.334
3.317IleThr: 3.317 ± 0.424
3.649IleVal: 3.649 ± 0.448
0.387IleTrp: 0.387 ± 0.125
0.719IleTyr: 0.719 ± 0.191
0.0IleXaa: 0.0 ± 0.0
Lys
3.538LysAla: 3.538 ± 0.459
0.442LysCys: 0.442 ± 0.163
1.88LysAsp: 1.88 ± 0.363
1.05LysGlu: 1.05 ± 0.197
1.161LysPhe: 1.161 ± 0.288
2.764LysGly: 2.764 ± 0.358
0.885LysHis: 0.885 ± 0.288
1.603LysIle: 1.603 ± 0.33
1.548LysLys: 1.548 ± 0.272
2.488LysLeu: 2.488 ± 0.317
0.663LysMet: 0.663 ± 0.184
0.663LysAsn: 0.663 ± 0.196
2.101LysPro: 2.101 ± 0.357
0.995LysGln: 0.995 ± 0.257
3.372LysArg: 3.372 ± 0.579
1.327LysSer: 1.327 ± 0.317
1.714LysThr: 1.714 ± 0.358
2.267LysVal: 2.267 ± 0.386
0.885LysTrp: 0.885 ± 0.173
0.553LysTyr: 0.553 ± 0.182
0.0LysXaa: 0.0 ± 0.0
Leu
10.669LeuAla: 10.669 ± 1.063
0.995LeuCys: 0.995 ± 0.223
4.865LeuAsp: 4.865 ± 0.569
4.644LeuGlu: 4.644 ± 0.385
2.156LeuPhe: 2.156 ± 0.318
6.855LeuGly: 6.855 ± 0.638
1.548LeuHis: 1.548 ± 0.391
3.427LeuIle: 3.427 ± 0.368
2.377LeuLys: 2.377 ± 0.49
6.413LeuLeu: 6.413 ± 0.545
1.658LeuMet: 1.658 ± 0.328
2.764LeuAsn: 2.764 ± 0.444
6.357LeuPro: 6.357 ± 0.594
2.819LeuGln: 2.819 ± 0.428
6.523LeuArg: 6.523 ± 0.67
3.704LeuSer: 3.704 ± 0.39
5.805LeuThr: 5.805 ± 0.526
6.302LeuVal: 6.302 ± 0.596
1.437LeuTrp: 1.437 ± 0.279
1.603LeuTyr: 1.603 ± 0.332
0.0LeuXaa: 0.0 ± 0.0
Met
2.875MetAla: 2.875 ± 0.338
0.332MetCys: 0.332 ± 0.125
1.161MetAsp: 1.161 ± 0.231
1.161MetGlu: 1.161 ± 0.301
0.332MetPhe: 0.332 ± 0.112
1.327MetGly: 1.327 ± 0.339
0.498MetHis: 0.498 ± 0.175
0.885MetIle: 0.885 ± 0.225
0.608MetLys: 0.608 ± 0.198
1.327MetLeu: 1.327 ± 0.344
0.276MetMet: 0.276 ± 0.14
0.663MetAsn: 0.663 ± 0.199
1.824MetPro: 1.824 ± 0.315
0.663MetGln: 0.663 ± 0.165
1.437MetArg: 1.437 ± 0.245
2.156MetSer: 2.156 ± 0.332
2.211MetThr: 2.211 ± 0.295
0.719MetVal: 0.719 ± 0.195
0.111MetTrp: 0.111 ± 0.078
0.276MetTyr: 0.276 ± 0.113
0.0MetXaa: 0.0 ± 0.0
Asn
3.206AsnAla: 3.206 ± 0.364
0.221AsnCys: 0.221 ± 0.113
1.493AsnAsp: 1.493 ± 0.263
1.327AsnGlu: 1.327 ± 0.279
0.498AsnPhe: 0.498 ± 0.175
2.985AsnGly: 2.985 ± 0.471
0.498AsnHis: 0.498 ± 0.168
0.885AsnIle: 0.885 ± 0.23
0.608AsnLys: 0.608 ± 0.162
2.598AsnLeu: 2.598 ± 0.44
0.663AsnMet: 0.663 ± 0.168
1.161AsnAsn: 1.161 ± 0.256
3.096AsnPro: 3.096 ± 0.41
0.608AsnGln: 0.608 ± 0.174
2.488AsnArg: 2.488 ± 0.388
1.548AsnSer: 1.548 ± 0.369
1.437AsnThr: 1.437 ± 0.234
1.824AsnVal: 1.824 ± 0.255
0.719AsnTrp: 0.719 ± 0.158
0.94AsnTyr: 0.94 ± 0.202
0.0AsnXaa: 0.0 ± 0.0
Pro
6.523ProAla: 6.523 ± 0.808
0.498ProCys: 0.498 ± 0.171
5.749ProAsp: 5.749 ± 0.645
5.141ProGlu: 5.141 ± 0.624
1.493ProPhe: 1.493 ± 0.323
6.413ProGly: 6.413 ± 0.879
1.161ProHis: 1.161 ± 0.227
2.875ProIle: 2.875 ± 0.308
1.935ProLys: 1.935 ± 0.377
4.699ProLeu: 4.699 ± 0.564
1.493ProMet: 1.493 ± 0.348
1.493ProAsn: 1.493 ± 0.264
4.478ProPro: 4.478 ± 0.689
2.156ProGln: 2.156 ± 0.407
3.814ProArg: 3.814 ± 0.56
3.262ProSer: 3.262 ± 0.487
4.865ProThr: 4.865 ± 0.507
4.367ProVal: 4.367 ± 0.535
1.327ProTrp: 1.327 ± 0.247
1.161ProTyr: 1.161 ± 0.222
0.0ProXaa: 0.0 ± 0.0
Gln
4.533GlnAla: 4.533 ± 0.718
0.387GlnCys: 0.387 ± 0.152
1.603GlnAsp: 1.603 ± 0.281
1.493GlnGlu: 1.493 ± 0.285
0.829GlnPhe: 0.829 ± 0.181
2.93GlnGly: 2.93 ± 0.52
0.829GlnHis: 0.829 ± 0.196
2.045GlnIle: 2.045 ± 0.277
0.995GlnLys: 0.995 ± 0.256
3.925GlnLeu: 3.925 ± 0.513
0.553GlnMet: 0.553 ± 0.181
1.05GlnAsn: 1.05 ± 0.194
2.377GlnPro: 2.377 ± 0.394
1.548GlnGln: 1.548 ± 0.44
3.704GlnArg: 3.704 ± 0.478
1.658GlnSer: 1.658 ± 0.283
1.603GlnThr: 1.603 ± 0.329
2.819GlnVal: 2.819 ± 0.409
0.774GlnTrp: 0.774 ± 0.215
0.498GlnTyr: 0.498 ± 0.266
0.0GlnXaa: 0.0 ± 0.0
Arg
7.85ArgAla: 7.85 ± 0.752
0.94ArgCys: 0.94 ± 0.304
4.699ArgAsp: 4.699 ± 0.52
3.98ArgGlu: 3.98 ± 0.512
2.101ArgPhe: 2.101 ± 0.313
5.639ArgGly: 5.639 ± 0.606
2.267ArgHis: 2.267 ± 0.417
3.206ArgIle: 3.206 ± 0.385
2.598ArgLys: 2.598 ± 0.396
6.136ArgLeu: 6.136 ± 0.653
2.322ArgMet: 2.322 ± 0.293
2.267ArgAsn: 2.267 ± 0.338
3.096ArgPro: 3.096 ± 0.422
2.93ArgGln: 2.93 ± 0.418
7.353ArgArg: 7.353 ± 0.909
3.704ArgSer: 3.704 ± 0.491
3.814ArgThr: 3.814 ± 0.428
4.975ArgVal: 4.975 ± 0.56
1.769ArgTrp: 1.769 ± 0.35
1.935ArgTyr: 1.935 ± 0.402
0.0ArgXaa: 0.0 ± 0.0
Ser
5.97SerAla: 5.97 ± 0.776
0.663SerCys: 0.663 ± 0.228
4.091SerAsp: 4.091 ± 0.452
2.322SerGlu: 2.322 ± 0.343
1.493SerPhe: 1.493 ± 0.323
4.533SerGly: 4.533 ± 0.593
0.885SerHis: 0.885 ± 0.242
2.101SerIle: 2.101 ± 0.385
1.658SerLys: 1.658 ± 0.35
3.98SerLeu: 3.98 ± 0.382
1.548SerMet: 1.548 ± 0.303
1.382SerAsn: 1.382 ± 0.23
3.372SerPro: 3.372 ± 0.461
2.156SerGln: 2.156 ± 0.312
2.709SerArg: 2.709 ± 0.465
2.432SerSer: 2.432 ± 0.403
2.985SerThr: 2.985 ± 0.349
3.262SerVal: 3.262 ± 0.418
1.271SerTrp: 1.271 ± 0.239
0.995SerTyr: 0.995 ± 0.246
0.0SerXaa: 0.0 ± 0.0
Thr
6.966ThrAla: 6.966 ± 0.627
0.553ThrCys: 0.553 ± 0.205
4.533ThrAsp: 4.533 ± 0.491
3.593ThrGlu: 3.593 ± 0.417
2.101ThrPhe: 2.101 ± 0.299
5.362ThrGly: 5.362 ± 0.713
1.548ThrHis: 1.548 ± 0.283
2.709ThrIle: 2.709 ± 0.33
2.985ThrLys: 2.985 ± 0.41
5.141ThrLeu: 5.141 ± 0.414
0.995ThrMet: 0.995 ± 0.226
1.548ThrAsn: 1.548 ± 0.356
5.473ThrPro: 5.473 ± 0.576
2.322ThrGln: 2.322 ± 0.349
3.096ThrArg: 3.096 ± 0.463
3.372ThrSer: 3.372 ± 0.399
3.372ThrThr: 3.372 ± 0.412
4.699ThrVal: 4.699 ± 0.631
1.548ThrTrp: 1.548 ± 0.285
1.769ThrTyr: 1.769 ± 0.31
0.0ThrXaa: 0.0 ± 0.0
Val
7.629ValAla: 7.629 ± 0.565
0.719ValCys: 0.719 ± 0.186
5.086ValAsp: 5.086 ± 0.489
4.699ValGlu: 4.699 ± 0.541
1.824ValPhe: 1.824 ± 0.249
5.197ValGly: 5.197 ± 0.603
1.106ValHis: 1.106 ± 0.22
2.985ValIle: 2.985 ± 0.4
1.658ValLys: 1.658 ± 0.272
4.423ValLeu: 4.423 ± 0.526
1.216ValMet: 1.216 ± 0.224
2.045ValAsn: 2.045 ± 0.371
4.146ValPro: 4.146 ± 0.362
2.709ValGln: 2.709 ± 0.317
6.192ValArg: 6.192 ± 0.693
3.372ValSer: 3.372 ± 0.394
5.694ValThr: 5.694 ± 0.662
5.86ValVal: 5.86 ± 0.782
1.437ValTrp: 1.437 ± 0.31
1.714ValTyr: 1.714 ± 0.307
0.0ValXaa: 0.0 ± 0.0
Trp
2.211TrpAla: 2.211 ± 0.398
0.442TrpCys: 0.442 ± 0.141
1.106TrpAsp: 1.106 ± 0.203
1.382TrpGlu: 1.382 ± 0.259
0.885TrpPhe: 0.885 ± 0.215
1.216TrpGly: 1.216 ± 0.236
0.719TrpHis: 0.719 ± 0.173
0.719TrpIle: 0.719 ± 0.21
0.608TrpLys: 0.608 ± 0.178
1.824TrpLeu: 1.824 ± 0.23
0.608TrpMet: 0.608 ± 0.197
0.332TrpAsn: 0.332 ± 0.151
0.995TrpPro: 0.995 ± 0.234
0.774TrpGln: 0.774 ± 0.216
1.658TrpArg: 1.658 ± 0.322
1.437TrpSer: 1.437 ± 0.265
1.493TrpThr: 1.493 ± 0.34
1.382TrpVal: 1.382 ± 0.288
0.553TrpTrp: 0.553 ± 0.166
0.608TrpTyr: 0.608 ± 0.178
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.151TyrAla: 3.151 ± 0.427
0.221TyrCys: 0.221 ± 0.118
1.824TyrAsp: 1.824 ± 0.297
2.045TyrGlu: 2.045 ± 0.336
0.553TyrPhe: 0.553 ± 0.167
1.824TyrGly: 1.824 ± 0.261
0.498TyrHis: 0.498 ± 0.138
0.885TyrIle: 0.885 ± 0.196
0.332TyrLys: 0.332 ± 0.136
2.045TyrLeu: 2.045 ± 0.372
0.111TyrMet: 0.111 ± 0.071
0.553TyrAsn: 0.553 ± 0.182
1.769TyrPro: 1.769 ± 0.356
1.106TyrGln: 1.106 ± 0.222
1.658TyrArg: 1.658 ± 0.274
1.327TyrSer: 1.327 ± 0.245
1.106TyrThr: 1.106 ± 0.249
1.935TyrVal: 1.935 ± 0.349
0.498TyrTrp: 0.498 ± 0.156
0.719TyrTyr: 0.719 ± 0.219
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 89 proteins (18090 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski