Tortoise microvirus 13

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W5S1|A0A4P8W5S1_9VIRU DNA pilot protein OS=Tortoise microvirus 13 OX=2583114 PE=4 SV=1
MM1 pKa = 7.18KK2 pKa = 9.67TVKK5 pKa = 10.49LPTYY9 pKa = 8.02TGRR12 pKa = 11.84MQPVEE17 pKa = 4.24CFEE20 pKa = 4.52GEE22 pKa = 4.26TIEE25 pKa = 4.56EE26 pKa = 4.18KK27 pKa = 11.02VNRR30 pKa = 11.84VVNNNEE36 pKa = 4.55PITDD40 pKa = 4.24GAPIIFTEE48 pKa = 4.43KK49 pKa = 10.33KK50 pKa = 10.42DD51 pKa = 3.98GVLPEE56 pKa = 3.96YY57 pKa = 10.7NIRR60 pKa = 11.84TDD62 pKa = 2.96RR63 pKa = 11.84WDD65 pKa = 3.47VALTAMEE72 pKa = 4.97KK73 pKa = 9.6MDD75 pKa = 4.0QARR78 pKa = 11.84KK79 pKa = 9.34AKK81 pKa = 10.24KK82 pKa = 9.01EE83 pKa = 3.94NAVKK87 pKa = 10.69PEE89 pKa = 4.08DD90 pKa = 3.9FGKK93 pKa = 10.29DD94 pKa = 3.37VPNKK98 pKa = 10.62LEE100 pKa = 4.26GGTPSEE106 pKa = 4.34NN107 pKa = 3.16

Molecular weight: 12.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W9K7|A0A4P8W9K7_9VIRU Replication initiation protein OS=Tortoise microvirus 13 OX=2583114 PE=4 SV=1
MM1 pKa = 7.12IKK3 pKa = 9.96KK4 pKa = 9.38RR5 pKa = 11.84KK6 pKa = 9.05KK7 pKa = 8.97RR8 pKa = 11.84AIYY11 pKa = 10.21SIGRR15 pKa = 11.84KK16 pKa = 6.88TFNTFKK22 pKa = 10.93AMKK25 pKa = 9.16TFVWYY30 pKa = 10.41NSHH33 pKa = 6.31TDD35 pKa = 3.04EE36 pKa = 4.28KK37 pKa = 10.46TEE39 pKa = 3.94GFEE42 pKa = 5.29IIDD45 pKa = 4.65DD46 pKa = 5.21EE47 pKa = 4.4IQKK50 pKa = 10.66HH51 pKa = 5.2YY52 pKa = 11.02IFTKK56 pKa = 9.51KK57 pKa = 8.81EE58 pKa = 3.55RR59 pKa = 11.84RR60 pKa = 11.84LLCEE64 pKa = 4.35KK65 pKa = 10.26IYY67 pKa = 11.08DD68 pKa = 3.75KK69 pKa = 11.69NEE71 pKa = 3.67EE72 pKa = 3.87HH73 pKa = 6.86KK74 pKa = 10.75KK75 pKa = 8.71WIKK78 pKa = 10.48SIQLNLFNNGG88 pKa = 3.0

Molecular weight: 10.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1803

67

657

200.3

23.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.214 ± 1.217

1.276 ± 0.443

4.437 ± 0.633

9.04 ± 1.733

2.773 ± 0.651

6.267 ± 0.918

1.719 ± 0.489

8.098 ± 1.082

9.928 ± 1.729

6.767 ± 0.839

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.161 ± 0.418

7.543 ± 1.065

2.385 ± 0.717

4.215 ± 1.035

4.049 ± 0.575

4.548 ± 0.715

7.82 ± 1.074

5.103 ± 0.615

2.108 ± 0.221

3.55 ± 0.515
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski