candidate division MSBL1 archaeon SCGC-AAA261O19

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 779 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A133VEJ6|A0A133VEJ6_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA261O19 OX=1698277 GN=AKJ48_01075 PE=4 SV=1
MM1 pKa = 7.71PNVIYY6 pKa = 10.26DD7 pKa = 3.61YY8 pKa = 11.37GKK10 pKa = 10.58CNGNASCAEE19 pKa = 4.0VCPVNILEE27 pKa = 4.15EE28 pKa = 4.49SEE30 pKa = 3.73NGRR33 pKa = 11.84WCKK36 pKa = 10.44PIDD39 pKa = 3.4EE40 pKa = 4.37KK41 pKa = 11.71VEE43 pKa = 3.81NEE45 pKa = 3.9EE46 pKa = 4.12AVKK49 pKa = 10.41QFHH52 pKa = 6.6EE53 pKa = 4.48EE54 pKa = 4.11VEE56 pKa = 4.24EE57 pKa = 5.14SEE59 pKa = 4.43TQLDD63 pKa = 3.79VTIEE67 pKa = 3.95NDD69 pKa = 3.31MPEE72 pKa = 5.31CIQCMACVASCPEE85 pKa = 3.87DD86 pKa = 4.59AIQIEE91 pKa = 4.38

Molecular weight:
10.23 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A133VD18|A0A133VD18_9EURY Ornithine carbamoyltransferase OS=candidate division MSBL1 archaeon SCGC-AAA261O19 OX=1698277 GN=AKJ48_02975 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 10.43SVGGTPRR9 pKa = 11.84FYY11 pKa = 10.28PKK13 pKa = 10.52EE14 pKa = 4.47GITLRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84GSWAWTEE28 pKa = 3.84MLFDD32 pKa = 5.57LMVDD36 pKa = 3.59PQRR39 pKa = 11.84WLRR42 pKa = 11.84EE43 pKa = 3.56YY44 pKa = 10.3HH45 pKa = 5.65VRR47 pKa = 11.84SNVEE51 pKa = 3.79SGFSIFTRR59 pKa = 11.84DD60 pKa = 3.18FLAPLRR66 pKa = 11.84KK67 pKa = 9.18RR68 pKa = 11.84IHH70 pKa = 6.32RR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84KK74 pKa = 7.27TEE76 pKa = 3.61AFARR80 pKa = 11.84TCDD83 pKa = 3.73YY84 pKa = 11.17NLKK87 pKa = 7.93QACYY91 pKa = 10.14ARR93 pKa = 11.84HH94 pKa = 5.27QEE96 pKa = 4.06GLIAPWMNTT105 pKa = 2.93

Molecular weight:
12.61 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

779

0

779

190618

50

1136

244.7

27.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.876 ± 0.093

0.965 ± 0.036

5.485 ± 0.081

9.471 ± 0.113

3.708 ± 0.061

7.659 ± 0.086

1.829 ± 0.038

7.209 ± 0.079

7.179 ± 0.113

9.752 ± 0.086

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.216 ± 0.035

3.346 ± 0.06

4.264 ± 0.054

2.459 ± 0.042

5.716 ± 0.083

6.025 ± 0.08

4.934 ± 0.058

6.974 ± 0.071

1.114 ± 0.036

2.817 ± 0.053

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski