Amino acid dipepetide frequency for Bacillus salarius

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.475AlaAla: 6.475 ± 0.083
0.624AlaCys: 0.624 ± 0.02
4.037AlaAsp: 4.037 ± 0.052
5.458AlaGlu: 5.458 ± 0.067
3.3AlaPhe: 3.3 ± 0.047
5.729AlaGly: 5.729 ± 0.057
1.305AlaHis: 1.305 ± 0.029
4.98AlaIle: 4.98 ± 0.05
3.77AlaLys: 3.77 ± 0.05
6.974AlaLeu: 6.974 ± 0.064
2.092AlaMet: 2.092 ± 0.038
2.667AlaAsn: 2.667 ± 0.035
2.212AlaPro: 2.212 ± 0.039
2.255AlaGln: 2.255 ± 0.035
2.601AlaArg: 2.601 ± 0.038
4.357AlaSer: 4.357 ± 0.053
3.269AlaThr: 3.269 ± 0.045
5.929AlaVal: 5.929 ± 0.059
0.623AlaTrp: 0.623 ± 0.02
2.258AlaTyr: 2.258 ± 0.032
0.0AlaXaa: 0.0 ± 0.0
Cys
0.431CysAla: 0.431 ± 0.02
0.094CysCys: 0.094 ± 0.008
0.376CysAsp: 0.376 ± 0.015
0.422CysGlu: 0.422 ± 0.013
0.318CysPhe: 0.318 ± 0.014
0.684CysGly: 0.684 ± 0.023
0.191CysHis: 0.191 ± 0.011
0.444CysIle: 0.444 ± 0.015
0.305CysLys: 0.305 ± 0.013
0.651CysLeu: 0.651 ± 0.017
0.171CysMet: 0.171 ± 0.012
0.248CysAsn: 0.248 ± 0.011
0.321CysPro: 0.321 ± 0.014
0.224CysGln: 0.224 ± 0.012
0.315CysArg: 0.315 ± 0.013
0.487CysSer: 0.487 ± 0.017
0.363CysThr: 0.363 ± 0.012
0.407CysVal: 0.407 ± 0.015
0.076CysTrp: 0.076 ± 0.006
0.217CysTyr: 0.217 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
3.557AspAla: 3.557 ± 0.047
0.338AspCys: 0.338 ± 0.015
3.14AspAsp: 3.14 ± 0.049
5.08AspGlu: 5.08 ± 0.068
2.309AspPhe: 2.309 ± 0.032
3.664AspGly: 3.664 ± 0.057
1.224AspHis: 1.224 ± 0.028
4.676AspIle: 4.676 ± 0.05
3.247AspLys: 3.247 ± 0.042
4.862AspLeu: 4.862 ± 0.053
1.745AspMet: 1.745 ± 0.03
2.419AspAsn: 2.419 ± 0.039
2.042AspPro: 2.042 ± 0.03
2.042AspGln: 2.042 ± 0.031
2.202AspArg: 2.202 ± 0.035
3.125AspSer: 3.125 ± 0.046
3.026AspThr: 3.026 ± 0.037
4.408AspVal: 4.408 ± 0.056
0.744AspTrp: 0.744 ± 0.022
2.231AspTyr: 2.231 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
6.001GluAla: 6.001 ± 0.063
0.398GluCys: 0.398 ± 0.017
5.03GluAsp: 5.03 ± 0.07
9.244GluGlu: 9.244 ± 0.101
2.335GluPhe: 2.335 ± 0.031
4.896GluGly: 4.896 ± 0.057
1.609GluHis: 1.609 ± 0.027
4.99GluIle: 4.99 ± 0.054
6.586GluLys: 6.586 ± 0.069
6.768GluLeu: 6.768 ± 0.064
2.612GluMet: 2.612 ± 0.04
4.547GluAsn: 4.547 ± 0.057
2.249GluPro: 2.249 ± 0.036
3.937GluGln: 3.937 ± 0.052
3.746GluArg: 3.746 ± 0.05
4.276GluSer: 4.276 ± 0.055
4.913GluThr: 4.913 ± 0.06
5.15GluVal: 5.15 ± 0.057
1.041GluTrp: 1.041 ± 0.02
2.273GluTyr: 2.273 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
2.885PheAla: 2.885 ± 0.042
0.324PheCys: 0.324 ± 0.014
2.321PheAsp: 2.321 ± 0.032
2.911PheGlu: 2.911 ± 0.038
2.423PhePhe: 2.423 ± 0.043
3.147PheGly: 3.147 ± 0.049
1.069PheHis: 1.069 ± 0.028
3.648PheIle: 3.648 ± 0.054
1.812PheLys: 1.812 ± 0.037
4.425PheLeu: 4.425 ± 0.058
1.217PheMet: 1.217 ± 0.026
1.657PheAsn: 1.657 ± 0.028
1.666PhePro: 1.666 ± 0.03
1.787PheGln: 1.787 ± 0.031
1.609PheArg: 1.609 ± 0.031
3.319PheSer: 3.319 ± 0.049
2.534PheThr: 2.534 ± 0.036
3.019PheVal: 3.019 ± 0.037
0.475PheTrp: 0.475 ± 0.016
1.659PheTyr: 1.659 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
5.053GlyAla: 5.053 ± 0.066
0.575GlyCys: 0.575 ± 0.019
3.472GlyAsp: 3.472 ± 0.048
5.236GlyGlu: 5.236 ± 0.062
3.371GlyPhe: 3.371 ± 0.047
5.077GlyGly: 5.077 ± 0.064
1.385GlyHis: 1.385 ± 0.029
5.556GlyIle: 5.556 ± 0.065
4.185GlyLys: 4.185 ± 0.048
6.415GlyLeu: 6.415 ± 0.066
2.392GlyMet: 2.392 ± 0.033
2.675GlyAsn: 2.675 ± 0.041
1.905GlyPro: 1.905 ± 0.035
2.162GlyGln: 2.162 ± 0.036
2.733GlyArg: 2.733 ± 0.04
4.283GlySer: 4.283 ± 0.051
4.155GlyThr: 4.155 ± 0.047
5.176GlyVal: 5.176 ± 0.057
0.853GlyTrp: 0.853 ± 0.023
2.663GlyTyr: 2.663 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
1.366HisAla: 1.366 ± 0.027
0.215HisCys: 0.215 ± 0.011
1.144HisAsp: 1.144 ± 0.026
1.46HisGlu: 1.46 ± 0.029
1.08HisPhe: 1.08 ± 0.025
1.461HisGly: 1.461 ± 0.029
0.767HisHis: 0.767 ± 0.026
1.542HisIle: 1.542 ± 0.029
0.981HisLys: 0.981 ± 0.023
2.173HisLeu: 2.173 ± 0.036
0.546HisMet: 0.546 ± 0.016
0.788HisAsn: 0.788 ± 0.02
1.179HisPro: 1.179 ± 0.027
0.894HisGln: 0.894 ± 0.018
0.967HisArg: 0.967 ± 0.026
1.364HisSer: 1.364 ± 0.027
1.212HisThr: 1.212 ± 0.026
1.557HisVal: 1.557 ± 0.03
0.261HisTrp: 0.261 ± 0.011
0.937HisTyr: 0.937 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
5.489IleAla: 5.489 ± 0.051
0.566IleCys: 0.566 ± 0.02
4.224IleAsp: 4.224 ± 0.05
5.336IleGlu: 5.336 ± 0.051
3.042IlePhe: 3.042 ± 0.055
5.714IleGly: 5.714 ± 0.063
1.612IleHis: 1.612 ± 0.031
5.361IleIle: 5.361 ± 0.067
3.617IleLys: 3.617 ± 0.046
6.235IleLeu: 6.235 ± 0.063
1.81IleMet: 1.81 ± 0.031
3.093IleAsn: 3.093 ± 0.042
3.201IlePro: 3.201 ± 0.045
2.701IleGln: 2.701 ± 0.039
2.865IleArg: 2.865 ± 0.038
4.881IleSer: 4.881 ± 0.063
4.322IleThr: 4.322 ± 0.047
5.103IleVal: 5.103 ± 0.057
0.644IleTrp: 0.644 ± 0.019
2.252IleTyr: 2.252 ± 0.038
0.0IleXaa: 0.0 ± 0.0
Lys
4.198LysAla: 4.198 ± 0.047
0.242LysCys: 0.242 ± 0.011
3.482LysAsp: 3.482 ± 0.044
6.566LysGlu: 6.566 ± 0.066
1.369LysPhe: 1.369 ± 0.025
3.853LysGly: 3.853 ± 0.048
1.343LysHis: 1.343 ± 0.031
3.616LysIle: 3.616 ± 0.045
5.04LysLys: 5.04 ± 0.054
4.336LysLeu: 4.336 ± 0.046
1.91LysMet: 1.91 ± 0.032
3.081LysAsn: 3.081 ± 0.043
1.948LysPro: 1.948 ± 0.033
3.166LysGln: 3.166 ± 0.046
3.048LysArg: 3.048 ± 0.043
3.035LysSer: 3.035 ± 0.043
3.466LysThr: 3.466 ± 0.042
3.825LysVal: 3.825 ± 0.045
0.711LysTrp: 0.711 ± 0.018
1.734LysTyr: 1.734 ± 0.031
0.0LysXaa: 0.0 ± 0.0
Leu
6.604LeuAla: 6.604 ± 0.068
0.632LeuCys: 0.632 ± 0.018
4.954LeuAsp: 4.954 ± 0.056
6.959LeuGlu: 6.959 ± 0.064
4.628LeuPhe: 4.628 ± 0.07
6.047LeuGly: 6.047 ± 0.075
2.16LeuHis: 2.16 ± 0.037
6.289LeuIle: 6.289 ± 0.06
5.357LeuLys: 5.357 ± 0.056
9.511LeuLeu: 9.511 ± 0.092
2.48LeuMet: 2.48 ± 0.038
3.899LeuAsn: 3.899 ± 0.053
3.874LeuPro: 3.874 ± 0.048
3.857LeuGln: 3.857 ± 0.05
3.633LeuArg: 3.633 ± 0.05
6.638LeuSer: 6.638 ± 0.062
5.358LeuThr: 5.358 ± 0.051
5.679LeuVal: 5.679 ± 0.061
0.81LeuTrp: 0.81 ± 0.022
3.055LeuTyr: 3.055 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
2.226MetAla: 2.226 ± 0.039
0.134MetCys: 0.134 ± 0.009
1.697MetAsp: 1.697 ± 0.031
2.489MetGlu: 2.489 ± 0.036
1.126MetPhe: 1.126 ± 0.028
1.866MetGly: 1.866 ± 0.032
0.486MetHis: 0.486 ± 0.014
2.167MetIle: 2.167 ± 0.036
2.356MetLys: 2.356 ± 0.037
2.646MetLeu: 2.646 ± 0.037
0.968MetMet: 0.968 ± 0.022
1.68MetAsn: 1.68 ± 0.031
1.03MetPro: 1.03 ± 0.026
1.095MetGln: 1.095 ± 0.026
1.114MetArg: 1.114 ± 0.026
1.865MetSer: 1.865 ± 0.036
1.838MetThr: 1.838 ± 0.032
1.858MetVal: 1.858 ± 0.03
0.213MetTrp: 0.213 ± 0.01
0.802MetTyr: 0.802 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
2.783AsnAla: 2.783 ± 0.04
0.251AsnCys: 0.251 ± 0.011
2.572AsnAsp: 2.572 ± 0.045
3.824AsnGlu: 3.824 ± 0.049
1.447AsnPhe: 1.447 ± 0.024
3.274AsnGly: 3.274 ± 0.045
1.138AsnHis: 1.138 ± 0.026
3.442AsnIle: 3.442 ± 0.048
2.801AsnLys: 2.801 ± 0.041
3.368AsnLeu: 3.368 ± 0.041
1.301AsnMet: 1.301 ± 0.027
2.232AsnAsn: 2.232 ± 0.048
2.024AsnPro: 2.024 ± 0.032
2.161AsnGln: 2.161 ± 0.04
2.022AsnArg: 2.022 ± 0.032
2.061AsnSer: 2.061 ± 0.036
2.34AsnThr: 2.34 ± 0.039
3.34AsnVal: 3.34 ± 0.045
0.517AsnTrp: 0.517 ± 0.016
1.406AsnTyr: 1.406 ± 0.028
0.0AsnXaa: 0.0 ± 0.0
Pro
2.548ProAla: 2.548 ± 0.039
0.221ProCys: 0.221 ± 0.011
2.449ProAsp: 2.449 ± 0.032
3.414ProGlu: 3.414 ± 0.041
1.927ProPhe: 1.927 ± 0.035
2.493ProGly: 2.493 ± 0.041
0.806ProHis: 0.806 ± 0.023
2.482ProIle: 2.482 ± 0.041
1.697ProLys: 1.697 ± 0.033
3.492ProLeu: 3.492 ± 0.037
0.957ProMet: 0.957 ± 0.024
1.51ProAsn: 1.51 ± 0.03
1.149ProPro: 1.149 ± 0.028
1.101ProGln: 1.101 ± 0.025
1.15ProArg: 1.15 ± 0.028
2.415ProSer: 2.415 ± 0.038
1.777ProThr: 1.777 ± 0.028
3.108ProVal: 3.108 ± 0.039
0.407ProTrp: 0.407 ± 0.013
1.403ProTyr: 1.403 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
3.039GlnAla: 3.039 ± 0.041
0.196GlnCys: 0.196 ± 0.01
2.013GlnAsp: 2.013 ± 0.035
3.524GlnGlu: 3.524 ± 0.049
1.513GlnPhe: 1.513 ± 0.028
2.412GlnGly: 2.412 ± 0.039
0.868GlnHis: 0.868 ± 0.022
2.245GlnIle: 2.245 ± 0.036
2.511GlnLys: 2.511 ± 0.04
3.761GlnLeu: 3.761 ± 0.055
1.241GlnMet: 1.241 ± 0.025
1.708GlnAsn: 1.708 ± 0.032
1.318GlnPro: 1.318 ± 0.026
2.148GlnGln: 2.148 ± 0.048
1.721GlnArg: 1.721 ± 0.032
2.424GlnSer: 2.424 ± 0.035
2.454GlnThr: 2.454 ± 0.038
2.471GlnVal: 2.471 ± 0.038
0.53GlnTrp: 0.53 ± 0.016
1.209GlnTyr: 1.209 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
2.47ArgAla: 2.47 ± 0.04
0.258ArgCys: 0.258 ± 0.012
2.207ArgAsp: 2.207 ± 0.037
3.3ArgGlu: 3.3 ± 0.045
1.957ArgPhe: 1.957 ± 0.033
2.366ArgGly: 2.366 ± 0.034
0.944ArgHis: 0.944 ± 0.025
2.846ArgIle: 2.846 ± 0.039
2.853ArgLys: 2.853 ± 0.037
4.05ArgLeu: 4.05 ± 0.046
1.336ArgMet: 1.336 ± 0.027
1.86ArgAsn: 1.86 ± 0.03
1.338ArgPro: 1.338 ± 0.025
1.699ArgGln: 1.699 ± 0.032
1.937ArgArg: 1.937 ± 0.037
2.438ArgSer: 2.438 ± 0.04
2.163ArgThr: 2.163 ± 0.034
2.525ArgVal: 2.525 ± 0.039
0.469ArgTrp: 0.469 ± 0.015
1.616ArgTyr: 1.616 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
4.074SerAla: 4.074 ± 0.049
0.377SerCys: 0.377 ± 0.015
3.365SerAsp: 3.365 ± 0.054
4.404SerGlu: 4.404 ± 0.054
3.427SerPhe: 3.427 ± 0.046
4.515SerGly: 4.515 ± 0.054
1.336SerHis: 1.336 ± 0.027
4.92SerIle: 4.92 ± 0.054
3.222SerLys: 3.222 ± 0.04
6.255SerLeu: 6.255 ± 0.065
2.012SerMet: 2.012 ± 0.028
2.653SerAsn: 2.653 ± 0.044
2.2SerPro: 2.2 ± 0.036
2.119SerGln: 2.119 ± 0.036
2.374SerArg: 2.374 ± 0.035
4.44SerSer: 4.44 ± 0.057
3.365SerThr: 3.365 ± 0.046
4.349SerVal: 4.349 ± 0.047
0.72SerTrp: 0.72 ± 0.021
2.271SerTyr: 2.271 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
4.353ThrAla: 4.353 ± 0.048
0.362ThrCys: 0.362 ± 0.013
3.116ThrAsp: 3.116 ± 0.045
4.162ThrGlu: 4.162 ± 0.044
2.882ThrPhe: 2.882 ± 0.041
4.526ThrGly: 4.526 ± 0.05
1.022ThrHis: 1.022 ± 0.024
4.305ThrIle: 4.305 ± 0.051
2.964ThrLys: 2.964 ± 0.037
5.312ThrLeu: 5.312 ± 0.051
1.551ThrMet: 1.551 ± 0.029
2.43ThrAsn: 2.43 ± 0.034
2.407ThrPro: 2.407 ± 0.033
1.558ThrGln: 1.558 ± 0.028
1.93ThrArg: 1.93 ± 0.034
3.586ThrSer: 3.586 ± 0.05
3.137ThrThr: 3.137 ± 0.045
4.201ThrVal: 4.201 ± 0.048
0.572ThrTrp: 0.572 ± 0.018
1.979ThrTyr: 1.979 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
4.68ValAla: 4.68 ± 0.062
0.556ValCys: 0.556 ± 0.017
3.92ValAsp: 3.92 ± 0.052
5.324ValGlu: 5.324 ± 0.069
3.207ValPhe: 3.207 ± 0.04
4.449ValGly: 4.449 ± 0.049
1.478ValHis: 1.478 ± 0.028
5.4ValIle: 5.4 ± 0.058
4.039ValLys: 4.039 ± 0.05
6.898ValLeu: 6.898 ± 0.058
1.997ValMet: 1.997 ± 0.034
3.074ValAsn: 3.074 ± 0.041
2.721ValPro: 2.721 ± 0.035
2.542ValGln: 2.542 ± 0.037
2.703ValArg: 2.703 ± 0.038
4.716ValSer: 4.716 ± 0.048
4.368ValThr: 4.368 ± 0.046
5.088ValVal: 5.088 ± 0.063
0.672ValTrp: 0.672 ± 0.019
2.247ValTyr: 2.247 ± 0.037
0.0ValXaa: 0.0 ± 0.0
Trp
0.624TrpAla: 0.624 ± 0.02
0.076TrpCys: 0.076 ± 0.006
0.536TrpAsp: 0.536 ± 0.016
0.832TrpGlu: 0.832 ± 0.021
0.53TrpPhe: 0.53 ± 0.019
0.706TrpGly: 0.706 ± 0.021
0.228TrpHis: 0.228 ± 0.011
0.81TrpIle: 0.81 ± 0.021
0.794TrpLys: 0.794 ± 0.022
1.149TrpLeu: 1.149 ± 0.033
0.425TrpMet: 0.425 ± 0.017
0.589TrpAsn: 0.589 ± 0.019
0.302TrpPro: 0.302 ± 0.015
0.426TrpGln: 0.426 ± 0.015
0.428TrpArg: 0.428 ± 0.017
0.668TrpSer: 0.668 ± 0.018
0.57TrpThr: 0.57 ± 0.016
0.669TrpVal: 0.669 ± 0.018
0.154TrpTrp: 0.154 ± 0.009
0.357TrpTyr: 0.357 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.057TyrAla: 2.057 ± 0.034
0.306TyrCys: 0.306 ± 0.012
2.027TyrAsp: 2.027 ± 0.034
2.658TyrGlu: 2.658 ± 0.037
1.715TyrPhe: 1.715 ± 0.029
2.443TyrGly: 2.443 ± 0.037
0.922TyrHis: 0.922 ± 0.023
2.344TyrIle: 2.344 ± 0.034
1.831TyrLys: 1.831 ± 0.036
3.143TyrLeu: 3.143 ± 0.039
0.894TyrMet: 0.894 ± 0.023
1.495TyrAsn: 1.495 ± 0.032
1.421TyrPro: 1.421 ± 0.029
1.432TyrGln: 1.432 ± 0.031
1.538TyrArg: 1.538 ± 0.025
2.023TyrSer: 2.023 ± 0.037
1.724TyrThr: 1.724 ± 0.026
2.216TyrVal: 2.216 ± 0.034
0.379TyrTrp: 0.379 ± 0.014
1.39TyrTyr: 1.39 ± 0.029
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6761 proteins (1924827 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski