Oxytricha trifallax

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata; Spirotrichea; Stichotrichia; Sporadotrichida; Oxytrichidae; Oxytrichinae; Oxytricha

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23559 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9IE20|J9IE20_9SPIT Uncharacterized protein OS=Oxytricha trifallax OX=1172189 GN=OXYTRI_01707 PE=4 SV=1
MM1 pKa = 7.38LFVYY5 pKa = 10.09KK6 pKa = 10.4NCKK9 pKa = 9.58LLNQVIRR16 pKa = 11.84FSPLFKK22 pKa = 10.36LKK24 pKa = 10.71SIMRR28 pKa = 11.84LAKK31 pKa = 10.12VLALTVVGLIATVGCQDD48 pKa = 3.15QVEE51 pKa = 4.87PGTEE55 pKa = 3.92DD56 pKa = 3.14QQQPEE61 pKa = 4.24IIIPGEE67 pKa = 3.88EE68 pKa = 4.14YY69 pKa = 10.8LRR71 pKa = 11.84AVTGLANSMSSHH83 pKa = 7.46AIPGGVAANITFALQNLSTYY103 pKa = 9.89YY104 pKa = 10.88DD105 pKa = 3.64SFSGFGQLLNQDD117 pKa = 3.75QTVGQYY123 pKa = 11.01LYY125 pKa = 9.66DD126 pKa = 3.58TYY128 pKa = 10.38KK129 pKa = 9.73TQISKK134 pKa = 10.5QDD136 pKa = 3.5DD137 pKa = 3.83AVFGLVDD144 pKa = 4.27RR145 pKa = 11.84NLKK148 pKa = 8.63TLFEE152 pKa = 4.57GQDD155 pKa = 2.98DD156 pKa = 4.87CYY158 pKa = 11.61NDD160 pKa = 3.78FSRR163 pKa = 11.84GIAEE167 pKa = 5.16IFLGWSKK174 pKa = 10.8LWDD177 pKa = 4.91RR178 pKa = 11.84ITGVNRR184 pKa = 11.84YY185 pKa = 8.84GKK187 pKa = 10.52LEE189 pKa = 3.97EE190 pKa = 4.74SKK192 pKa = 11.06DD193 pKa = 3.66FADD196 pKa = 3.83FCVRR200 pKa = 11.84EE201 pKa = 3.88RR202 pKa = 11.84DD203 pKa = 3.81NLSSHH208 pKa = 6.92IDD210 pKa = 4.17DD211 pKa = 4.31IADD214 pKa = 3.41MLLGTEE220 pKa = 4.05QCDD223 pKa = 3.72VLQALYY229 pKa = 10.72NGNVQQNIWTGWRR242 pKa = 11.84DD243 pKa = 3.74NIDD246 pKa = 3.17NNAAYY251 pKa = 10.39LVMYY255 pKa = 7.61FTEE258 pKa = 4.91GVAVFDD264 pKa = 4.2SCHH267 pKa = 5.58EE268 pKa = 4.34VYY270 pKa = 10.34QQLDD274 pKa = 3.1ADD276 pKa = 4.78FAADD280 pKa = 3.38EE281 pKa = 4.43TQQTEE286 pKa = 4.1HH287 pKa = 6.34QAQLDD292 pKa = 3.59QYY294 pKa = 9.7RR295 pKa = 11.84TDD297 pKa = 3.81FQDD300 pKa = 2.64IVNRR304 pKa = 11.84ILEE307 pKa = 4.14FDD309 pKa = 3.44QLALHH314 pKa = 6.6SIEE317 pKa = 4.45LTLPQNLVQLLDD329 pKa = 3.9EE330 pKa = 4.71NQDD333 pKa = 3.21LSLEE337 pKa = 4.24VMGFNVNGFFLSTIYY352 pKa = 9.98WDD354 pKa = 4.17YY355 pKa = 10.99LMQMGAVFSDD365 pKa = 3.31ASEE368 pKa = 3.86DD369 pKa = 3.6AFYY372 pKa = 11.15SEE374 pKa = 5.17CWQCFTVTQGDD385 pKa = 3.98YY386 pKa = 10.6RR387 pKa = 11.84VTVSWLPVEE396 pKa = 4.66GTASVTQEE404 pKa = 3.89TLDD407 pKa = 4.37AIVFDD412 pKa = 4.12EE413 pKa = 4.59TNSKK417 pKa = 10.52EE418 pKa = 4.27DD419 pKa = 3.5LANQVWLGMGDD430 pKa = 3.42EE431 pKa = 4.6CVAGVMIFDD440 pKa = 4.45RR441 pKa = 11.84SIDD444 pKa = 3.67HH445 pKa = 6.22YY446 pKa = 11.82VNTQFTDD453 pKa = 3.31QVVFSQPDD461 pKa = 3.51DD462 pKa = 3.87VDD464 pKa = 4.3IIAWGINEE472 pKa = 4.4LCLGRR477 pKa = 11.84PSLGSTQPVEE487 pKa = 4.06EE488 pKa = 4.35QPTVEE493 pKa = 4.56TPTPADD499 pKa = 3.63TEE501 pKa = 4.2ATTDD505 pKa = 3.58NNEE508 pKa = 4.18EE509 pKa = 4.24SSDD512 pKa = 3.78TQPP515 pKa = 4.01

Molecular weight: 58.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9HMT3|J9HMT3_9SPIT Serine/threonine protein kinase OS=Oxytricha trifallax OX=1172189 GN=OXYTRI_14793 PE=4 SV=1
MM1 pKa = 7.52ARR3 pKa = 11.84TAAPKK8 pKa = 10.04RR9 pKa = 11.84VAKK12 pKa = 10.51RR13 pKa = 11.84PTKK16 pKa = 10.27KK17 pKa = 10.47AGSKK21 pKa = 10.11KK22 pKa = 8.73IAKK25 pKa = 9.98RR26 pKa = 11.84PVAKK30 pKa = 10.33KK31 pKa = 10.37AGAKK35 pKa = 9.23RR36 pKa = 11.84VAKK39 pKa = 10.28KK40 pKa = 8.88ATPVAAAPVVAGSTSATPQKK60 pKa = 10.72AAAKK64 pKa = 9.94KK65 pKa = 9.12SAKK68 pKa = 9.72KK69 pKa = 9.58AAKK72 pKa = 10.08RR73 pKa = 11.84PAKK76 pKa = 9.97KK77 pKa = 9.91AAPKK81 pKa = 10.26RR82 pKa = 11.84RR83 pKa = 3.57

Molecular weight: 8.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23559

0

23559

15273496

49

21680

648.3

74.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.901 ± 0.011

1.15 ± 0.009

5.628 ± 0.009

6.216 ± 0.02

4.569 ± 0.011

4.179 ± 0.016

1.915 ± 0.007

7.208 ± 0.014

8.4 ± 0.022

8.675 ± 0.013

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.443 ± 0.006

7.941 ± 0.017

3.11 ± 0.012

8.963 ± 0.022

3.851 ± 0.012

8.233 ± 0.016

4.91 ± 0.021

4.407 ± 0.01

0.579 ± 0.004

3.72 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski