Amino acid dipepetide frequency for Oxytricha trifallax

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	1.983AlaAla: 1.983 ± 0.019	0.513AlaCys: 0.513 ± 0.009	1.862AlaAsp: 1.862 ± 0.012	1.959AlaGlu: 1.959 ± 0.014	1.996AlaPhe: 1.996 ± 0.014	1.792AlaGly: 1.792 ± 0.02	0.721AlaHis: 0.721 ± 0.007	2.889AlaIle: 2.889 ± 0.019	3.182AlaLys: 3.182 ± 0.017	3.698AlaLeu: 3.698 ± 0.021	0.93AlaMet: 0.93 ± 0.008	2.819AlaAsn: 2.819 ± 0.015	1.196AlaPro: 1.196 ± 0.022	3.213AlaGln: 3.213 ± 0.015	1.414AlaArg: 1.414 ± 0.012	3.158AlaSer: 3.158 ± 0.018	2.033AlaThr: 2.033 ± 0.016	1.923AlaVal: 1.923 ± 0.015	0.23AlaTrp: 0.23 ± 0.004	1.499AlaTyr: 1.499 ± 0.012	0.001AlaXaa: 0.001 ± 0.0
Cys	0.446CysAla: 0.446 ± 0.007	0.236CysCys: 0.236 ± 0.005	0.659CysAsp: 0.659 ± 0.01	0.667CysGlu: 0.667 ± 0.008	0.604CysPhe: 0.604 ± 0.008	0.635CysGly: 0.635 ± 0.01	0.217CysHis: 0.217 ± 0.004	0.777CysIle: 0.777 ± 0.009	0.881CysLys: 0.881 ± 0.011	1.154CysLeu: 1.154 ± 0.013	0.27CysMet: 0.27 ± 0.005	0.676CysAsn: 0.676 ± 0.01	0.508CysPro: 0.508 ± 0.021	0.805CysGln: 0.805 ± 0.011	0.395CysArg: 0.395 ± 0.006	0.871CysSer: 0.871 ± 0.013	0.578CysThr: 0.578 ± 0.012	0.529CysVal: 0.529 ± 0.008	0.101CysTrp: 0.101 ± 0.003	0.491CysTyr: 0.491 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	1.906AspAla: 1.906 ± 0.012	0.666AspCys: 0.666 ± 0.008	3.777AspAsp: 3.777 ± 0.026	4.235AspGlu: 4.235 ± 0.021	3.033AspPhe: 3.033 ± 0.015	2.251AspGly: 2.251 ± 0.023	1.043AspHis: 1.043 ± 0.009	4.133AspIle: 4.133 ± 0.019	4.476AspLys: 4.476 ± 0.022	5.328AspLeu: 5.328 ± 0.019	1.287AspMet: 1.287 ± 0.01	3.6AspAsn: 3.6 ± 0.016	1.863AspPro: 1.863 ± 0.013	4.948AspGln: 4.948 ± 0.021	1.967AspArg: 1.967 ± 0.014	4.386AspSer: 4.386 ± 0.02	2.302AspThr: 2.302 ± 0.015	2.261AspVal: 2.261 ± 0.014	0.375AspTrp: 0.375 ± 0.006	2.446AspTyr: 2.446 ± 0.013	0.001AspXaa: 0.001 ± 0.0
Glu	2.364GluAla: 2.364 ± 0.016	0.713GluCys: 0.713 ± 0.009	4.128GluAsp: 4.128 ± 0.023	5.779GluGlu: 5.779 ± 0.035	2.755GluPhe: 2.755 ± 0.015	2.257GluGly: 2.257 ± 0.015	0.973GluHis: 0.973 ± 0.009	4.933GluIle: 4.933 ± 0.02	5.677GluLys: 5.677 ± 0.03	5.224GluLeu: 5.224 ± 0.024	1.601GluMet: 1.601 ± 0.011	4.591GluAsn: 4.591 ± 0.023	1.196GluPro: 1.196 ± 0.01	4.737GluGln: 4.737 ± 0.027	2.731GluArg: 2.731 ± 0.019	4.311GluSer: 4.311 ± 0.017	2.687GluThr: 2.687 ± 0.016	2.859GluVal: 2.859 ± 0.017	0.386GluTrp: 0.386 ± 0.007	2.254GluTyr: 2.254 ± 0.012	0.001GluXaa: 0.001 ± 0.0
Phe	1.782PheAla: 1.782 ± 0.013	0.589PheCys: 0.589 ± 0.008	3.034PheAsp: 3.034 ± 0.017	2.994PheGlu: 2.994 ± 0.017	2.03PhePhe: 2.03 ± 0.017	2.296PheGly: 2.296 ± 0.015	0.792PheHis: 0.792 ± 0.008	3.262PheIle: 3.262 ± 0.021	3.919PheLys: 3.919 ± 0.015	3.723PheLeu: 3.723 ± 0.023	1.26PheMet: 1.26 ± 0.01	3.582PheAsn: 3.582 ± 0.016	1.152PhePro: 1.152 ± 0.01	3.43PheGln: 3.43 ± 0.018	1.593PheArg: 1.593 ± 0.012	3.539PheSer: 3.539 ± 0.018	2.428PheThr: 2.428 ± 0.017	2.071PheVal: 2.071 ± 0.014	0.313PheTrp: 0.313 ± 0.005	1.897PheTyr: 1.897 ± 0.013	0.001PheXaa: 0.001 ± 0.0
Gly	1.896GlyAla: 1.896 ± 0.018	0.556GlyCys: 0.556 ± 0.009	2.275GlyAsp: 2.275 ± 0.019	2.014GlyGlu: 2.014 ± 0.016	2.188GlyPhe: 2.188 ± 0.017	3.208GlyGly: 3.208 ± 0.036	0.779GlyHis: 0.779 ± 0.008	2.894GlyIle: 2.894 ± 0.016	3.049GlyLys: 3.049 ± 0.018	3.497GlyLeu: 3.497 ± 0.02	1.059GlyMet: 1.059 ± 0.009	2.959GlyAsn: 2.959 ± 0.015	0.987GlyPro: 0.987 ± 0.011	2.943GlyGln: 2.943 ± 0.019	1.529GlyArg: 1.529 ± 0.011	3.644GlySer: 3.644 ± 0.025	2.077GlyThr: 2.077 ± 0.022	2.183GlyVal: 2.183 ± 0.016	0.313GlyTrp: 0.313 ± 0.008	1.741GlyTyr: 1.741 ± 0.019	0.001GlyXaa: 0.001 ± 0.0
His	0.643HisAla: 0.643 ± 0.007	0.217HisCys: 0.217 ± 0.004	0.943HisAsp: 0.943 ± 0.01	1.109HisGlu: 1.109 ± 0.009	0.901HisPhe: 0.901 ± 0.009	0.763HisGly: 0.763 ± 0.008	0.623HisHis: 0.623 ± 0.009	1.246HisIle: 1.246 ± 0.011	1.521HisLys: 1.521 ± 0.013	1.785HisLeu: 1.785 ± 0.012	0.397HisMet: 0.397 ± 0.005	1.421HisAsn: 1.421 ± 0.013	0.694HisPro: 0.694 ± 0.007	2.051HisGln: 2.051 ± 0.017	0.781HisArg: 0.781 ± 0.008	1.665HisSer: 1.665 ± 0.013	0.833HisThr: 0.833 ± 0.007	0.692HisVal: 0.692 ± 0.007	0.109HisTrp: 0.109 ± 0.002	0.757HisTyr: 0.757 ± 0.008	0.0HisXaa: 0.0 ± 0.0
Ile	2.847IleAla: 2.847 ± 0.015	0.899IleCys: 0.899 ± 0.009	4.625IleAsp: 4.625 ± 0.018	4.867IleGlu: 4.867 ± 0.022	3.234IlePhe: 3.234 ± 0.022	2.917IleGly: 2.917 ± 0.018	1.282IleHis: 1.282 ± 0.01	4.986IleIle: 4.986 ± 0.023	6.285IleLys: 6.285 ± 0.024	6.104IleLeu: 6.104 ± 0.025	1.73IleMet: 1.73 ± 0.011	5.791IleAsn: 5.791 ± 0.022	2.328IlePro: 2.328 ± 0.014	6.048IleGln: 6.048 ± 0.026	2.567IleArg: 2.567 ± 0.014	5.708IleSer: 5.708 ± 0.025	3.407IleThr: 3.407 ± 0.027	3.343IleVal: 3.343 ± 0.017	0.433IleTrp: 0.433 ± 0.006	2.677IleTyr: 2.677 ± 0.017	0.001IleXaa: 0.001 ± 0.0
Lys	3.245LysAla: 3.245 ± 0.017	0.927LysCys: 0.927 ± 0.014	4.72LysAsp: 4.72 ± 0.022	5.641LysGlu: 5.641 ± 0.033	3.575LysPhe: 3.575 ± 0.017	2.717LysGly: 2.717 ± 0.015	1.664LysHis: 1.664 ± 0.013	6.358LysIle: 6.358 ± 0.027	7.735LysLys: 7.735 ± 0.044	7.585LysLeu: 7.585 ± 0.031	1.974LysMet: 1.974 ± 0.013	6.345LysAsn: 6.345 ± 0.026	2.52LysPro: 2.52 ± 0.016	7.256LysGln: 7.256 ± 0.034	3.678LysArg: 3.678 ± 0.022	6.713LysSer: 6.713 ± 0.031	3.914LysThr: 3.914 ± 0.017	3.891LysVal: 3.891 ± 0.017	0.468LysTrp: 0.468 ± 0.007	3.076LysTyr: 3.076 ± 0.017	0.001LysXaa: 0.001 ± 0.0
Leu	3.571LeuAla: 3.571 ± 0.017	0.964LeuCys: 0.964 ± 0.011	4.904LeuAsp: 4.904 ± 0.022	5.603LeuGlu: 5.603 ± 0.026	3.603LeuPhe: 3.603 ± 0.022	3.474LeuGly: 3.474 ± 0.017	1.546LeuHis: 1.546 ± 0.013	6.358LeuIle: 6.358 ± 0.025	8.216LeuLys: 8.216 ± 0.035	7.14LeuLeu: 7.14 ± 0.036	2.282LeuMet: 2.282 ± 0.014	7.287LeuAsn: 7.287 ± 0.025	2.707LeuPro: 2.707 ± 0.016	7.035LeuGln: 7.035 ± 0.032	3.746LeuArg: 3.746 ± 0.02	6.841LeuSer: 6.841 ± 0.025	4.394LeuThr: 4.394 ± 0.026	3.741LeuVal: 3.741 ± 0.021	0.483LeuTrp: 0.483 ± 0.005	2.853LeuTyr: 2.853 ± 0.017	0.0LeuXaa: 0.0 ± 0.0
Met	0.944MetAla: 0.944 ± 0.007	0.237MetCys: 0.237 ± 0.004	1.177MetAsp: 1.177 ± 0.009	1.355MetGlu: 1.355 ± 0.01	1.003MetPhe: 1.003 ± 0.008	1.045MetGly: 1.045 ± 0.009	0.485MetHis: 0.485 ± 0.005	2.006MetIle: 2.006 ± 0.013	2.346MetLys: 2.346 ± 0.013	2.011MetLeu: 2.011 ± 0.012	0.825MetMet: 0.825 ± 0.01	2.108MetAsn: 2.108 ± 0.013	0.84MetPro: 0.84 ± 0.008	1.848MetGln: 1.848 ± 0.013	1.194MetArg: 1.194 ± 0.009	1.976MetSer: 1.976 ± 0.012	1.295MetThr: 1.295 ± 0.01	0.911MetVal: 0.911 ± 0.007	0.133MetTrp: 0.133 ± 0.003	0.694MetTyr: 0.694 ± 0.008	0.0MetXaa: 0.0 ± 0.0
Asn	2.624AsnAla: 2.624 ± 0.015	0.801AsnCys: 0.801 ± 0.011	4.034AsnAsp: 4.034 ± 0.018	4.413AsnGlu: 4.413 ± 0.021	3.674AsnPhe: 3.674 ± 0.016	3.075AsnGly: 3.075 ± 0.029	1.751AsnHis: 1.751 ± 0.013	5.467AsnIle: 5.467 ± 0.023	5.959AsnLys: 5.959 ± 0.027	7.31AsnLeu: 7.31 ± 0.026	1.82AsnMet: 1.82 ± 0.013	6.998AsnAsn: 6.998 ± 0.04	2.73AsnPro: 2.73 ± 0.016	8.786AsnGln: 8.786 ± 0.047	2.613AsnArg: 2.613 ± 0.015	7.172AsnSer: 7.172 ± 0.041	3.808AsnThr: 3.808 ± 0.022	2.868AsnVal: 2.868 ± 0.014	0.375AsnTrp: 0.375 ± 0.006	3.13AsnTyr: 3.13 ± 0.015	0.001AsnXaa: 0.001 ± 0.0
Pro	1.209ProAla: 1.209 ± 0.017	0.298ProCys: 0.298 ± 0.006	1.548ProAsp: 1.548 ± 0.012	1.733ProGlu: 1.733 ± 0.013	1.439ProPhe: 1.439 ± 0.01	1.234ProGly: 1.234 ± 0.015	0.61ProHis: 0.61 ± 0.007	2.309ProIle: 2.309 ± 0.016	2.431ProLys: 2.431 ± 0.013	2.713ProLeu: 2.713 ± 0.016	0.651ProMet: 0.651 ± 0.007	2.296ProAsn: 2.296 ± 0.014	1.294ProPro: 1.294 ± 0.02	2.925ProGln: 2.925 ± 0.016	1.14ProArg: 1.14 ± 0.01	2.755ProSer: 2.755 ± 0.021	1.737ProThr: 1.737 ± 0.017	1.442ProVal: 1.442 ± 0.014	0.17ProTrp: 0.17 ± 0.004	1.166ProTyr: 1.166 ± 0.01	0.0ProXaa: 0.0 ± 0.0
Gln	3.284GlnAla: 3.284 ± 0.017	0.726GlnCys: 0.726 ± 0.011	4.474GlnAsp: 4.474 ± 0.021	5.384GlnGlu: 5.384 ± 0.029	3.336GlnPhe: 3.336 ± 0.017	2.814GlnGly: 2.814 ± 0.019	1.634GlnHis: 1.634 ± 0.013	6.575GlnIle: 6.575 ± 0.028	7.403GlnLys: 7.403 ± 0.037	7.014GlnLeu: 7.014 ± 0.028	2.315GlnMet: 2.315 ± 0.016	8.863GlnAsn: 8.863 ± 0.049	2.47GlnPro: 2.47 ± 0.016	10.717GlnGln: 10.717 ± 0.089	3.45GlnArg: 3.45 ± 0.02	7.78GlnSer: 7.78 ± 0.039	4.471GlnThr: 4.471 ± 0.022	3.721GlnVal: 3.721 ± 0.016	0.371GlnTrp: 0.371 ± 0.006	2.829GlnTyr: 2.829 ± 0.015	0.001GlnXaa: 0.001 ± 0.0
Arg	1.524ArgAla: 1.524 ± 0.013	0.385ArgCys: 0.385 ± 0.007	2.45ArgAsp: 2.45 ± 0.015	2.686ArgGlu: 2.686 ± 0.021	1.694ArgPhe: 1.694 ± 0.013	1.695ArgGly: 1.695 ± 0.015	0.699ArgHis: 0.699 ± 0.008	2.684ArgIle: 2.684 ± 0.015	3.528ArgLys: 3.528 ± 0.02	3.261ArgLeu: 3.261 ± 0.018	0.97ArgMet: 0.97 ± 0.008	2.964ArgAsn: 2.964 ± 0.016	1.128ArgPro: 1.128 ± 0.01	3.088ArgGln: 3.088 ± 0.02	1.893ArgArg: 1.893 ± 0.015	2.806ArgSer: 2.806 ± 0.018	1.605ArgThr: 1.605 ± 0.011	1.893ArgVal: 1.893 ± 0.012	0.209ArgTrp: 0.209 ± 0.004	1.349ArgTyr: 1.349 ± 0.01	0.0ArgXaa: 0.0 ± 0.0
Ser	3.06SerAla: 3.06 ± 0.017	0.859SerCys: 0.859 ± 0.011	4.039SerAsp: 4.039 ± 0.025	3.961SerGlu: 3.961 ± 0.019	3.653SerPhe: 3.653 ± 0.017	3.475SerGly: 3.475 ± 0.019	1.72SerHis: 1.72 ± 0.014	5.626SerIle: 5.626 ± 0.02	6.684SerLys: 6.684 ± 0.032	6.965SerLeu: 6.965 ± 0.025	1.886SerMet: 1.886 ± 0.012	6.962SerAsn: 6.962 ± 0.039	2.942SerPro: 2.942 ± 0.025	8.357SerGln: 8.357 ± 0.041	3.06SerArg: 3.06 ± 0.019	8.061SerSer: 8.061 ± 0.044	4.392SerThr: 4.392 ± 0.029	3.4SerVal: 3.4 ± 0.017	0.394SerTrp: 0.394 ± 0.007	2.838SerTyr: 2.838 ± 0.021	0.001SerXaa: 0.001 ± 0.0
Thr	2.118ThrAla: 2.118 ± 0.019	0.66ThrCys: 0.66 ± 0.013	2.349ThrAsp: 2.349 ± 0.016	2.331ThrGlu: 2.331 ± 0.014	2.508ThrPhe: 2.508 ± 0.019	2.164ThrGly: 2.164 ± 0.024	0.967ThrHis: 0.967 ± 0.008	3.533ThrIle: 3.533 ± 0.022	3.597ThrLys: 3.597 ± 0.016	4.53ThrLeu: 4.53 ± 0.021	1.064ThrMet: 1.064 ± 0.009	3.783ThrAsn: 3.783 ± 0.023	1.934ThrPro: 1.934 ± 0.016	4.359ThrGln: 4.359 ± 0.017	1.543ThrArg: 1.543 ± 0.012	4.289ThrSer: 4.289 ± 0.032	2.941ThrThr: 2.941 ± 0.032	2.25ThrVal: 2.25 ± 0.022	0.285ThrTrp: 0.285 ± 0.005	1.894ThrTyr: 1.894 ± 0.021	0.0ThrXaa: 0.0 ± 0.0
Val	1.877ValAla: 1.877 ± 0.014	0.583ValCys: 0.583 ± 0.007	2.622ValAsp: 2.622 ± 0.015	2.669ValGlu: 2.669 ± 0.017	2.164ValPhe: 2.164 ± 0.015	1.969ValGly: 1.969 ± 0.015	0.768ValHis: 0.768 ± 0.007	3.237ValIle: 3.237 ± 0.016	3.882ValLys: 3.882 ± 0.021	3.7ValLeu: 3.7 ± 0.02	1.063ValMet: 1.063 ± 0.01	3.292ValAsn: 3.292 ± 0.015	1.404ValPro: 1.404 ± 0.014	3.403ValGln: 3.403 ± 0.017	1.632ValArg: 1.632 ± 0.013	3.35ValSer: 3.35 ± 0.018	2.193ValThr: 2.193 ± 0.023	2.232ValVal: 2.232 ± 0.017	0.305ValTrp: 0.305 ± 0.005	1.726ValTyr: 1.726 ± 0.013	0.0ValXaa: 0.0 ± 0.0
Trp	0.275TrpAla: 0.275 ± 0.004	0.082TrpCys: 0.082 ± 0.002	0.352TrpAsp: 0.352 ± 0.007	0.339TrpGlu: 0.339 ± 0.005	0.282TrpPhe: 0.282 ± 0.005	0.291TrpGly: 0.291 ± 0.007	0.11TrpHis: 0.11 ± 0.003	0.46TrpIle: 0.46 ± 0.006	0.483TrpLys: 0.483 ± 0.006	0.493TrpLeu: 0.493 ± 0.007	0.151TrpMet: 0.151 ± 0.003	0.425TrpAsn: 0.425 ± 0.006	0.154TrpPro: 0.154 ± 0.003	0.319TrpGln: 0.319 ± 0.005	0.279TrpArg: 0.279 ± 0.005	0.413TrpSer: 0.413 ± 0.009	0.305TrpThr: 0.305 ± 0.007	0.307TrpVal: 0.307 ± 0.005	0.056TrpTrp: 0.056 ± 0.002	0.212TrpTyr: 0.212 ± 0.004	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.411TyrAla: 1.411 ± 0.012	0.588TyrCys: 0.588 ± 0.013	2.312TyrAsp: 2.312 ± 0.014	2.418TyrGlu: 2.418 ± 0.014	2.014TyrPhe: 2.014 ± 0.014	1.721TyrGly: 1.721 ± 0.013	0.767TyrHis: 0.767 ± 0.008	2.345TyrIle: 2.345 ± 0.015	2.744TyrLys: 2.744 ± 0.015	3.515TyrLeu: 3.515 ± 0.02	0.898TyrMet: 0.898 ± 0.007	2.644TyrAsn: 2.644 ± 0.018	1.054TyrPro: 1.054 ± 0.009	3.362TyrGln: 3.362 ± 0.019	1.308TyrArg: 1.308 ± 0.016	2.898TyrSer: 2.898 ± 0.021	1.7TyrThr: 1.7 ± 0.015	1.552TyrVal: 1.552 ± 0.013	0.278TyrTrp: 0.278 ± 0.005	1.665TyrTyr: 1.665 ± 0.015	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.001XaaLeu: 0.001 ± 0.0	0.001XaaMet: 0.001 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.001XaaXaa: 0.001 ± 0.0

Statistics based on 23559 proteins (15273496 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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