Amino acid dipepetide frequency for Lachnospiraceae bacterium TWA4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.629AlaAla: 4.629 ± 0.102	1.093AlaCys: 1.093 ± 0.043	3.558AlaAsp: 3.558 ± 0.069	3.417AlaGlu: 3.417 ± 0.076	2.817AlaPhe: 2.817 ± 0.065	4.644AlaGly: 4.644 ± 0.095	1.023AlaHis: 1.023 ± 0.04	5.593AlaIle: 5.593 ± 0.099	5.583AlaLys: 5.583 ± 0.09	6.032AlaLeu: 6.032 ± 0.111	2.194AlaMet: 2.194 ± 0.05	2.84AlaAsn: 2.84 ± 0.067	1.757AlaPro: 1.757 ± 0.05	1.803AlaGln: 1.803 ± 0.053	2.362AlaArg: 2.362 ± 0.054	3.84AlaSer: 3.84 ± 0.077	3.569AlaThr: 3.569 ± 0.077	4.64AlaVal: 4.64 ± 0.098	0.544AlaTrp: 0.544 ± 0.027	2.528AlaTyr: 2.528 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	0.943CysAla: 0.943 ± 0.038	0.282CysCys: 0.282 ± 0.021	0.826CysAsp: 0.826 ± 0.037	1.03CysGlu: 1.03 ± 0.03	0.655CysPhe: 0.655 ± 0.025	1.41CysGly: 1.41 ± 0.05	0.335CysHis: 0.335 ± 0.023	1.197CysIle: 1.197 ± 0.044	1.182CysLys: 1.182 ± 0.043	1.083CysLeu: 1.083 ± 0.039	0.437CysMet: 0.437 ± 0.024	0.611CysAsn: 0.611 ± 0.028	0.633CysPro: 0.633 ± 0.032	0.44CysGln: 0.44 ± 0.021	0.538CysArg: 0.538 ± 0.027	0.906CysSer: 0.906 ± 0.038	0.853CysThr: 0.853 ± 0.031	1.058CysVal: 1.058 ± 0.041	0.087CysTrp: 0.087 ± 0.01	0.615CysTyr: 0.615 ± 0.029	0.0CysXaa: 0.0 ± 0.0
Asp	3.724AspAla: 3.724 ± 0.081	0.903AspCys: 0.903 ± 0.035	2.957AspAsp: 2.957 ± 0.075	5.151AspGlu: 5.151 ± 0.085	2.803AspPhe: 2.803 ± 0.062	3.918AspGly: 3.918 ± 0.081	0.882AspHis: 0.882 ± 0.033	4.461AspIle: 4.461 ± 0.077	4.05AspLys: 4.05 ± 0.075	5.12AspLeu: 5.12 ± 0.078	1.666AspMet: 1.666 ± 0.045	2.157AspAsn: 2.157 ± 0.049	1.486AspPro: 1.486 ± 0.042	1.305AspGln: 1.305 ± 0.04	1.892AspArg: 1.892 ± 0.047	3.447AspSer: 3.447 ± 0.064	2.979AspThr: 2.979 ± 0.065	3.738AspVal: 3.738 ± 0.065	0.573AspTrp: 0.573 ± 0.031	3.211AspTyr: 3.211 ± 0.068	0.0AspXaa: 0.0 ± 0.0
Glu	4.749GluAla: 4.749 ± 0.087	0.859GluCys: 0.859 ± 0.033	4.484GluAsp: 4.484 ± 0.073	7.191GluGlu: 7.191 ± 0.13	3.064GluPhe: 3.064 ± 0.065	4.258GluGly: 4.258 ± 0.079	1.366GluHis: 1.366 ± 0.045	5.936GluIle: 5.936 ± 0.089	6.39GluLys: 6.39 ± 0.107	7.248GluLeu: 7.248 ± 0.111	2.13GluMet: 2.13 ± 0.046	4.268GluAsn: 4.268 ± 0.079	1.633GluPro: 1.633 ± 0.05	2.746GluGln: 2.746 ± 0.063	2.804GluArg: 2.804 ± 0.063	3.745GluSer: 3.745 ± 0.071	3.617GluThr: 3.617 ± 0.073	5.215GluVal: 5.215 ± 0.093	0.648GluTrp: 0.648 ± 0.028	3.095GluTyr: 3.095 ± 0.064	0.0GluXaa: 0.0 ± 0.0
Phe	2.593PheAla: 2.593 ± 0.059	0.749PheCys: 0.749 ± 0.036	2.68PheAsp: 2.68 ± 0.06	2.847PheGlu: 2.847 ± 0.062	1.761PhePhe: 1.761 ± 0.047	2.936PheGly: 2.936 ± 0.062	0.81PheHis: 0.81 ± 0.03	3.441PheIle: 3.441 ± 0.065	2.719PheLys: 2.719 ± 0.066	3.692PheLeu: 3.692 ± 0.073	1.241PheMet: 1.241 ± 0.039	1.897PheAsn: 1.897 ± 0.05	1.37PhePro: 1.37 ± 0.042	1.025PheGln: 1.025 ± 0.032	1.435PheArg: 1.435 ± 0.041	2.913PheSer: 2.913 ± 0.055	2.412PheThr: 2.412 ± 0.059	2.706PheVal: 2.706 ± 0.054	0.381PheTrp: 0.381 ± 0.022	1.898PheTyr: 1.898 ± 0.055	0.0PheXaa: 0.0 ± 0.0
Gly	4.238GlyAla: 4.238 ± 0.109	1.278GlyCys: 1.278 ± 0.048	3.169GlyAsp: 3.169 ± 0.053	4.06GlyGlu: 4.06 ± 0.075	2.869GlyPhe: 2.869 ± 0.068	4.289GlyGly: 4.289 ± 0.086	1.231GlyHis: 1.231 ± 0.04	6.003GlyIle: 6.003 ± 0.102	5.516GlyLys: 5.516 ± 0.084	5.717GlyLeu: 5.717 ± 0.102	2.187GlyMet: 2.187 ± 0.057	3.008GlyAsn: 3.008 ± 0.061	1.217GlyPro: 1.217 ± 0.043	1.974GlyGln: 1.974 ± 0.054	2.595GlyArg: 2.595 ± 0.061	3.913GlySer: 3.913 ± 0.074	3.935GlyThr: 3.935 ± 0.086	4.805GlyVal: 4.805 ± 0.081	0.604GlyTrp: 0.604 ± 0.027	3.157GlyTyr: 3.157 ± 0.065	0.0GlyXaa: 0.0 ± 0.0
His	1.032HisAla: 1.032 ± 0.033	0.335HisCys: 0.335 ± 0.022	0.826HisAsp: 0.826 ± 0.032	1.164HisGlu: 1.164 ± 0.039	0.793HisPhe: 0.793 ± 0.037	1.183HisGly: 1.183 ± 0.042	0.421HisHis: 0.421 ± 0.037	1.31HisIle: 1.31 ± 0.043	1.128HisLys: 1.128 ± 0.035	1.77HisLeu: 1.77 ± 0.051	0.511HisMet: 0.511 ± 0.02	0.714HisAsn: 0.714 ± 0.032	0.855HisPro: 0.855 ± 0.034	0.601HisGln: 0.601 ± 0.031	0.766HisArg: 0.766 ± 0.031	1.143HisSer: 1.143 ± 0.041	1.026HisThr: 1.026 ± 0.035	1.045HisVal: 1.045 ± 0.042	0.167HisTrp: 0.167 ± 0.014	0.799HisTyr: 0.799 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	5.329IleAla: 5.329 ± 0.104	1.416IleCys: 1.416 ± 0.04	4.712IleAsp: 4.712 ± 0.077	5.845IleGlu: 5.845 ± 0.095	3.281IlePhe: 3.281 ± 0.068	5.377IleGly: 5.377 ± 0.091	1.508IleHis: 1.508 ± 0.05	5.766IleIle: 5.766 ± 0.101	5.453IleLys: 5.453 ± 0.08	7.585IleLeu: 7.585 ± 0.134	1.87IleMet: 1.87 ± 0.047	3.523IleAsn: 3.523 ± 0.066	3.242IlePro: 3.242 ± 0.071	2.441IleGln: 2.441 ± 0.052	3.201IleArg: 3.201 ± 0.063	5.471IleSer: 5.471 ± 0.088	4.332IleThr: 4.332 ± 0.079	5.325IleVal: 5.325 ± 0.084	0.609IleTrp: 0.609 ± 0.032	3.324IleTyr: 3.324 ± 0.067	0.0IleXaa: 0.0 ± 0.0
Lys	5.096LysAla: 5.096 ± 0.088	0.816LysCys: 0.816 ± 0.033	4.887LysAsp: 4.887 ± 0.083	8.095LysGlu: 8.095 ± 0.126	2.404LysPhe: 2.404 ± 0.055	4.423LysGly: 4.423 ± 0.078	1.178LysHis: 1.178 ± 0.041	5.811LysIle: 5.811 ± 0.084	7.326LysLys: 7.326 ± 0.117	6.38LysLeu: 6.38 ± 0.094	2.292LysMet: 2.292 ± 0.05	4.589LysAsn: 4.589 ± 0.083	2.176LysPro: 2.176 ± 0.054	2.686LysGln: 2.686 ± 0.06	3.124LysArg: 3.124 ± 0.071	4.38LysSer: 4.38 ± 0.085	4.211LysThr: 4.211 ± 0.08	5.167LysVal: 5.167 ± 0.074	0.621LysTrp: 0.621 ± 0.028	3.101LysTyr: 3.101 ± 0.067	0.0LysXaa: 0.0 ± 0.0
Leu	6.183LeuAla: 6.183 ± 0.094	1.452LeuCys: 1.452 ± 0.052	5.42LeuAsp: 5.42 ± 0.082	6.985LeuGlu: 6.985 ± 0.115	3.656LeuPhe: 3.656 ± 0.08	5.827LeuGly: 5.827 ± 0.096	1.539LeuHis: 1.539 ± 0.043	6.601LeuIle: 6.601 ± 0.108	7.318LeuLys: 7.318 ± 0.112	8.422LeuLeu: 8.422 ± 0.127	2.659LeuMet: 2.659 ± 0.062	4.255LeuAsn: 4.255 ± 0.075	3.362LeuPro: 3.362 ± 0.069	2.793LeuGln: 2.793 ± 0.056	3.322LeuArg: 3.322 ± 0.076	6.358LeuSer: 6.358 ± 0.088	4.978LeuThr: 4.978 ± 0.082	5.885LeuVal: 5.885 ± 0.082	0.764LeuTrp: 0.764 ± 0.027	3.347LeuTyr: 3.347 ± 0.065	0.0LeuXaa: 0.0 ± 0.0
Met	2.093MetAla: 2.093 ± 0.057	0.351MetCys: 0.351 ± 0.023	1.838MetAsp: 1.838 ± 0.051	2.126MetGlu: 2.126 ± 0.047	0.961MetPhe: 0.961 ± 0.036	2.051MetGly: 2.051 ± 0.053	0.443MetHis: 0.443 ± 0.022	2.414MetIle: 2.414 ± 0.052	2.602MetLys: 2.602 ± 0.058	2.252MetLeu: 2.252 ± 0.049	0.912MetMet: 0.912 ± 0.038	1.883MetAsn: 1.883 ± 0.041	0.967MetPro: 0.967 ± 0.035	0.94MetGln: 0.94 ± 0.039	1.112MetArg: 1.112 ± 0.036	1.868MetSer: 1.868 ± 0.043	1.736MetThr: 1.736 ± 0.051	1.892MetVal: 1.892 ± 0.05	0.177MetTrp: 0.177 ± 0.016	0.921MetTyr: 0.921 ± 0.041	0.0MetXaa: 0.0 ± 0.0
Asn	2.864AsnAla: 2.864 ± 0.067	0.765AsnCys: 0.765 ± 0.031	2.499AsnAsp: 2.499 ± 0.059	3.66AsnGlu: 3.66 ± 0.067	1.914AsnPhe: 1.914 ± 0.051	3.366AsnGly: 3.366 ± 0.073	1.1AsnHis: 1.1 ± 0.039	3.348AsnIle: 3.348 ± 0.071	3.522AsnLys: 3.522 ± 0.068	4.58AsnLeu: 4.58 ± 0.072	1.343AsnMet: 1.343 ± 0.042	2.194AsnAsn: 2.194 ± 0.06	2.181AsnPro: 2.181 ± 0.05	1.988AsnGln: 1.988 ± 0.055	2.076AsnArg: 2.076 ± 0.049	2.826AsnSer: 2.826 ± 0.066	2.429AsnThr: 2.429 ± 0.057	2.889AsnVal: 2.889 ± 0.067	0.46AsnTrp: 0.46 ± 0.027	2.36AsnTyr: 2.36 ± 0.057	0.0AsnXaa: 0.0 ± 0.0
Pro	1.748ProAla: 1.748 ± 0.05	0.404ProCys: 0.404 ± 0.023	1.736ProAsp: 1.736 ± 0.05	2.618ProGlu: 2.618 ± 0.054	1.515ProPhe: 1.515 ± 0.043	1.821ProGly: 1.821 ± 0.048	0.554ProHis: 0.554 ± 0.026	2.673ProIle: 2.673 ± 0.056	2.266ProLys: 2.266 ± 0.054	2.652ProLeu: 2.652 ± 0.067	0.938ProMet: 0.938 ± 0.034	1.526ProAsn: 1.526 ± 0.045	0.609ProPro: 0.609 ± 0.028	0.821ProGln: 0.821 ± 0.035	0.95ProArg: 0.95 ± 0.035	1.981ProSer: 1.981 ± 0.052	1.886ProThr: 1.886 ± 0.046	2.502ProVal: 2.502 ± 0.055	0.3ProTrp: 0.3 ± 0.02	1.449ProTyr: 1.449 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.143GlnAla: 2.143 ± 0.054	0.365GlnCys: 0.365 ± 0.022	1.293GlnAsp: 1.293 ± 0.043	2.188GlnGlu: 2.188 ± 0.06	1.242GlnPhe: 1.242 ± 0.043	1.709GlnGly: 1.709 ± 0.055	0.371GlnHis: 0.371 ± 0.021	2.873GlnIle: 2.873 ± 0.058	2.684GlnLys: 2.684 ± 0.054	3.248GlnLeu: 3.248 ± 0.066	1.206GlnMet: 1.206 ± 0.035	1.557GlnAsn: 1.557 ± 0.044	0.772GlnPro: 0.772 ± 0.029	0.914GlnGln: 0.914 ± 0.039	1.069GlnArg: 1.069 ± 0.034	1.793GlnSer: 1.793 ± 0.051	1.764GlnThr: 1.764 ± 0.051	2.307GlnVal: 2.307 ± 0.054	0.318GlnTrp: 0.318 ± 0.021	1.297GlnTyr: 1.297 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	2.135ArgAla: 2.135 ± 0.046	0.557ArgCys: 0.557 ± 0.026	1.787ArgAsp: 1.787 ± 0.046	3.069ArgGlu: 3.069 ± 0.062	1.624ArgPhe: 1.624 ± 0.044	2.07ArgGly: 2.07 ± 0.058	0.616ArgHis: 0.616 ± 0.03	3.153ArgIle: 3.153 ± 0.067	3.162ArgLys: 3.162 ± 0.071	3.502ArgLeu: 3.502 ± 0.073	1.324ArgMet: 1.324 ± 0.039	1.892ArgAsn: 1.892 ± 0.051	1.181ArgPro: 1.181 ± 0.038	1.427ArgGln: 1.427 ± 0.042	1.746ArgArg: 1.746 ± 0.054	1.924ArgSer: 1.924 ± 0.04	2.026ArgThr: 2.026 ± 0.044	2.403ArgVal: 2.403 ± 0.059	0.306ArgTrp: 0.306 ± 0.02	1.748ArgTyr: 1.748 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	3.662SerAla: 3.662 ± 0.074	0.826SerCys: 0.826 ± 0.03	3.446SerAsp: 3.446 ± 0.073	4.055SerGlu: 4.055 ± 0.073	2.752SerPhe: 2.752 ± 0.056	4.456SerGly: 4.456 ± 0.085	1.077SerHis: 1.077 ± 0.042	5.229SerIle: 5.229 ± 0.086	4.879SerLys: 4.879 ± 0.078	5.555SerLeu: 5.555 ± 0.082	1.881SerMet: 1.881 ± 0.047	3.04SerAsn: 3.04 ± 0.071	1.719SerPro: 1.719 ± 0.047	1.829SerGln: 1.829 ± 0.052	2.276SerArg: 2.276 ± 0.056	4.016SerSer: 4.016 ± 0.088	3.37SerThr: 3.37 ± 0.066	4.158SerVal: 4.158 ± 0.067	0.561SerTrp: 0.561 ± 0.027	2.765SerTyr: 2.765 ± 0.065	0.0SerXaa: 0.0 ± 0.0
Thr	3.629ThrAla: 3.629 ± 0.075	0.736ThrCys: 0.736 ± 0.032	3.168ThrAsp: 3.168 ± 0.079	3.251ThrGlu: 3.251 ± 0.072	2.231ThrPhe: 2.231 ± 0.055	4.167ThrGly: 4.167 ± 0.081	0.955ThrHis: 0.955 ± 0.034	4.761ThrIle: 4.761 ± 0.086	4.132ThrLys: 4.132 ± 0.068	4.946ThrLeu: 4.946 ± 0.09	1.443ThrMet: 1.443 ± 0.044	2.585ThrAsn: 2.585 ± 0.059	1.976ThrPro: 1.976 ± 0.052	1.491ThrGln: 1.491 ± 0.044	1.887ThrArg: 1.887 ± 0.042	3.438ThrSer: 3.438 ± 0.066	3.311ThrThr: 3.311 ± 0.081	4.107ThrVal: 4.107 ± 0.081	0.476ThrTrp: 0.476 ± 0.026	2.507ThrTyr: 2.507 ± 0.068	0.0ThrXaa: 0.0 ± 0.0
Val	4.704ValAla: 4.704 ± 0.092	1.128ValCys: 1.128 ± 0.044	4.116ValAsp: 4.116 ± 0.075	4.801ValGlu: 4.801 ± 0.086	2.897ValPhe: 2.897 ± 0.061	4.382ValGly: 4.382 ± 0.087	1.115ValHis: 1.115 ± 0.037	5.355ValIle: 5.355 ± 0.098	5.025ValLys: 5.025 ± 0.091	6.429ValLeu: 6.429 ± 0.104	1.819ValMet: 1.819 ± 0.049	3.184ValAsn: 3.184 ± 0.067	2.164ValPro: 2.164 ± 0.049	1.844ValGln: 1.844 ± 0.047	2.427ValArg: 2.427 ± 0.056	4.393ValSer: 4.393 ± 0.079	3.917ValThr: 3.917 ± 0.073	5.032ValVal: 5.032 ± 0.091	0.514ValTrp: 0.514 ± 0.026	2.67ValTyr: 2.67 ± 0.055	0.0ValXaa: 0.0 ± 0.0
Trp	0.485TrpAla: 0.485 ± 0.024	0.126TrpCys: 0.126 ± 0.013	0.51TrpAsp: 0.51 ± 0.03	0.579TrpGlu: 0.579 ± 0.029	0.404TrpPhe: 0.404 ± 0.022	0.556TrpGly: 0.556 ± 0.026	0.144TrpHis: 0.144 ± 0.013	0.716TrpIle: 0.716 ± 0.031	0.754TrpLys: 0.754 ± 0.032	0.783TrpLeu: 0.783 ± 0.032	0.331TrpMet: 0.331 ± 0.023	0.589TrpAsn: 0.589 ± 0.031	0.188TrpPro: 0.188 ± 0.016	0.294TrpGln: 0.294 ± 0.021	0.292TrpArg: 0.292 ± 0.018	0.493TrpSer: 0.493 ± 0.024	0.427TrpThr: 0.427 ± 0.027	0.464TrpVal: 0.464 ± 0.024	0.115TrpTrp: 0.115 ± 0.011	0.373TrpTyr: 0.373 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.485TyrAla: 2.485 ± 0.053	0.679TyrCys: 0.679 ± 0.029	2.51TyrAsp: 2.51 ± 0.048	3.314TyrGlu: 3.314 ± 0.072	1.861TyrPhe: 1.861 ± 0.051	2.922TyrGly: 2.922 ± 0.066	0.921TyrHis: 0.921 ± 0.033	2.969TyrIle: 2.969 ± 0.066	3.075TyrLys: 3.075 ± 0.066	4.127TyrLeu: 4.127 ± 0.082	1.155TyrMet: 1.155 ± 0.036	2.054TyrAsn: 2.054 ± 0.053	1.46TyrPro: 1.46 ± 0.034	1.749TyrGln: 1.749 ± 0.047	1.779TyrArg: 1.779 ± 0.049	2.707TyrSer: 2.707 ± 0.067	2.419TyrThr: 2.419 ± 0.066	2.579TyrVal: 2.579 ± 0.07	0.399TyrTrp: 0.399 ± 0.024	2.118TyrTyr: 2.118 ± 0.067	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2918 proteins (819781 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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