Amino acid dipepetide frequency for Clostridium oryzae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.262AlaAla: 5.262 ± 0.084	0.739AlaCys: 0.739 ± 0.027	3.153AlaAsp: 3.153 ± 0.044	4.024AlaGlu: 4.024 ± 0.059	2.917AlaPhe: 2.917 ± 0.052	4.059AlaGly: 4.059 ± 0.057	0.924AlaHis: 0.924 ± 0.027	5.498AlaIle: 5.498 ± 0.071	4.751AlaLys: 4.751 ± 0.06	5.878AlaLeu: 5.878 ± 0.068	1.71AlaMet: 1.71 ± 0.036	2.746AlaAsn: 2.746 ± 0.049	1.463AlaPro: 1.463 ± 0.038	1.598AlaGln: 1.598 ± 0.035	2.065AlaArg: 2.065 ± 0.037	3.795AlaSer: 3.795 ± 0.059	2.689AlaThr: 2.689 ± 0.054	4.844AlaVal: 4.844 ± 0.064	0.465AlaTrp: 0.465 ± 0.017	2.44AlaTyr: 2.44 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.688CysAla: 0.688 ± 0.025	0.24CysCys: 0.24 ± 0.015	0.746CysAsp: 0.746 ± 0.023	0.794CysGlu: 0.794 ± 0.023	0.576CysPhe: 0.576 ± 0.022	1.097CysGly: 1.097 ± 0.029	0.237CysHis: 0.237 ± 0.014	1.161CysIle: 1.161 ± 0.032	0.945CysLys: 0.945 ± 0.027	0.912CysLeu: 0.912 ± 0.026	0.331CysMet: 0.331 ± 0.018	0.699CysAsn: 0.699 ± 0.024	0.435CysPro: 0.435 ± 0.021	0.254CysGln: 0.254 ± 0.014	0.46CysArg: 0.46 ± 0.017	0.944CysSer: 0.944 ± 0.027	0.653CysThr: 0.653 ± 0.021	0.692CysVal: 0.692 ± 0.023	0.116CysTrp: 0.116 ± 0.009	0.528CysTyr: 0.528 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	2.97AspAla: 2.97 ± 0.046	0.668AspCys: 0.668 ± 0.022	2.89AspAsp: 2.89 ± 0.048	4.337AspGlu: 4.337 ± 0.063	2.786AspPhe: 2.786 ± 0.045	3.581AspGly: 3.581 ± 0.055	0.567AspHis: 0.567 ± 0.023	5.991AspIle: 5.991 ± 0.068	5.271AspLys: 5.271 ± 0.069	4.156AspLeu: 4.156 ± 0.057	1.683AspMet: 1.683 ± 0.035	3.335AspAsn: 3.335 ± 0.05	1.325AspPro: 1.325 ± 0.033	0.967AspGln: 0.967 ± 0.028	2.035AspArg: 2.035 ± 0.047	3.397AspSer: 3.397 ± 0.055	2.799AspThr: 2.799 ± 0.052	3.465AspVal: 3.465 ± 0.054	0.524AspTrp: 0.524 ± 0.024	2.818AspTyr: 2.818 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	4.271GluAla: 4.271 ± 0.057	0.67GluCys: 0.67 ± 0.022	3.722GluAsp: 3.722 ± 0.054	5.323GluGlu: 5.323 ± 0.078	2.747GluPhe: 2.747 ± 0.051	3.603GluGly: 3.603 ± 0.055	1.064GluHis: 1.064 ± 0.027	6.066GluIle: 6.066 ± 0.07	7.052GluLys: 7.052 ± 0.082	6.281GluLeu: 6.281 ± 0.076	1.831GluMet: 1.831 ± 0.035	4.617GluAsn: 4.617 ± 0.057	1.398GluPro: 1.398 ± 0.033	2.024GluGln: 2.024 ± 0.039	2.567GluArg: 2.567 ± 0.05	3.269GluSer: 3.269 ± 0.042	2.841GluThr: 2.841 ± 0.044	4.105GluVal: 4.105 ± 0.061	0.475GluTrp: 0.475 ± 0.017	3.005GluTyr: 3.005 ± 0.04	0.0GluXaa: 0.0 ± 0.0
Phe	2.417PheAla: 2.417 ± 0.045	0.627PheCys: 0.627 ± 0.022	2.553PheAsp: 2.553 ± 0.043	2.598PheGlu: 2.598 ± 0.051	2.038PhePhe: 2.038 ± 0.051	2.791PheGly: 2.791 ± 0.056	0.687PheHis: 0.687 ± 0.022	4.53PheIle: 4.53 ± 0.065	3.493PheLys: 3.493 ± 0.049	3.771PheLeu: 3.771 ± 0.055	1.229PheMet: 1.229 ± 0.031	2.845PheAsn: 2.845 ± 0.054	1.268PhePro: 1.268 ± 0.031	1.151PheGln: 1.151 ± 0.03	1.504PheArg: 1.504 ± 0.037	3.603PheSer: 3.603 ± 0.042	2.458PheThr: 2.458 ± 0.047	2.742PheVal: 2.742 ± 0.052	0.411PheTrp: 0.411 ± 0.015	1.945PheTyr: 1.945 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	3.906GlyAla: 3.906 ± 0.065	0.94GlyCys: 0.94 ± 0.029	3.231GlyAsp: 3.231 ± 0.05	3.796GlyGlu: 3.796 ± 0.065	3.041GlyPhe: 3.041 ± 0.053	3.856GlyGly: 3.856 ± 0.061	1.01GlyHis: 1.01 ± 0.029	6.459GlyIle: 6.459 ± 0.071	5.668GlyLys: 5.668 ± 0.07	4.994GlyLeu: 4.994 ± 0.067	1.782GlyMet: 1.782 ± 0.038	3.281GlyAsn: 3.281 ± 0.048	1.111GlyPro: 1.111 ± 0.032	1.578GlyGln: 1.578 ± 0.034	2.285GlyArg: 2.285 ± 0.05	3.792GlySer: 3.792 ± 0.05	3.576GlyThr: 3.576 ± 0.059	4.05GlyVal: 4.05 ± 0.061	0.585GlyTrp: 0.585 ± 0.022	3.119GlyTyr: 3.119 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	0.75HisAla: 0.75 ± 0.026	0.232HisCys: 0.232 ± 0.012	0.734HisAsp: 0.734 ± 0.024	0.89HisGlu: 0.89 ± 0.023	0.75HisPhe: 0.75 ± 0.024	1.123HisGly: 1.123 ± 0.032	0.319HisHis: 0.319 ± 0.017	1.453HisIle: 1.453 ± 0.031	1.17HisLys: 1.17 ± 0.029	1.241HisLeu: 1.241 ± 0.029	0.443HisMet: 0.443 ± 0.016	0.845HisAsn: 0.845 ± 0.028	0.643HisPro: 0.643 ± 0.021	0.383HisGln: 0.383 ± 0.016	0.587HisArg: 0.587 ± 0.02	0.96HisSer: 0.96 ± 0.028	0.757HisThr: 0.757 ± 0.023	0.824HisVal: 0.824 ± 0.028	0.173HisTrp: 0.173 ± 0.012	0.678HisTyr: 0.678 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.84IleAla: 5.84 ± 0.071	1.268IleCys: 1.268 ± 0.03	5.588IleAsp: 5.588 ± 0.061	6.186IleGlu: 6.186 ± 0.076	4.185IlePhe: 4.185 ± 0.062	5.762IleGly: 5.762 ± 0.067	1.239IleHis: 1.239 ± 0.032	8.577IleIle: 8.577 ± 0.102	7.897IleLys: 7.897 ± 0.084	8.239IleLeu: 8.239 ± 0.092	2.433IleMet: 2.433 ± 0.043	5.923IleAsn: 5.923 ± 0.073	3.202IlePro: 3.202 ± 0.059	2.21IleGln: 2.21 ± 0.036	3.225IleArg: 3.225 ± 0.047	6.943IleSer: 6.943 ± 0.085	4.867IleThr: 4.867 ± 0.061	5.979IleVal: 5.979 ± 0.074	0.636IleTrp: 0.636 ± 0.019	3.685IleTyr: 3.685 ± 0.058	0.0IleXaa: 0.0 ± 0.0
Lys	5.296LysAla: 5.296 ± 0.058	0.886LysCys: 0.886 ± 0.03	5.223LysAsp: 5.223 ± 0.069	6.73LysGlu: 6.73 ± 0.082	3.145LysPhe: 3.145 ± 0.047	4.786LysGly: 4.786 ± 0.063	1.332LysHis: 1.332 ± 0.026	7.62LysIle: 7.62 ± 0.081	7.974LysLys: 7.974 ± 0.099	8.088LysLeu: 8.088 ± 0.078	2.343LysMet: 2.343 ± 0.044	6.038LysAsn: 6.038 ± 0.073	2.242LysPro: 2.242 ± 0.041	2.739LysGln: 2.739 ± 0.043	3.156LysArg: 3.156 ± 0.045	5.168LysSer: 5.168 ± 0.073	4.129LysThr: 4.129 ± 0.06	5.506LysVal: 5.506 ± 0.067	0.753LysTrp: 0.753 ± 0.028	4.293LysTyr: 4.293 ± 0.06	0.0LysXaa: 0.0 ± 0.0
Leu	5.258LeuAla: 5.258 ± 0.075	1.19LeuCys: 1.19 ± 0.032	4.846LeuAsp: 4.846 ± 0.065	5.48LeuGlu: 5.48 ± 0.07	3.853LeuPhe: 3.853 ± 0.068	5.32LeuGly: 5.32 ± 0.06	1.281LeuHis: 1.281 ± 0.031	7.647LeuIle: 7.647 ± 0.098	8.12LeuLys: 8.12 ± 0.083	7.922LeuLeu: 7.922 ± 0.095	2.444LeuMet: 2.444 ± 0.04	5.403LeuAsn: 5.403 ± 0.068	2.96LeuPro: 2.96 ± 0.048	2.503LeuGln: 2.503 ± 0.04	3.277LeuArg: 3.277 ± 0.052	6.766LeuSer: 6.766 ± 0.078	4.491LeuThr: 4.491 ± 0.054	4.993LeuVal: 4.993 ± 0.057	0.699LeuTrp: 0.699 ± 0.023	3.482LeuTyr: 3.482 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	1.809MetAla: 1.809 ± 0.04	0.293MetCys: 0.293 ± 0.015	1.698MetAsp: 1.698 ± 0.039	1.907MetGlu: 1.907 ± 0.037	1.15MetPhe: 1.15 ± 0.031	1.639MetGly: 1.639 ± 0.035	0.412MetHis: 0.412 ± 0.019	2.112MetIle: 2.112 ± 0.043	2.537MetLys: 2.537 ± 0.038	2.751MetLeu: 2.751 ± 0.045	0.727MetMet: 0.727 ± 0.024	1.759MetAsn: 1.759 ± 0.03	0.953MetPro: 0.953 ± 0.028	0.779MetGln: 0.779 ± 0.023	0.982MetArg: 0.982 ± 0.027	1.82MetSer: 1.82 ± 0.033	1.269MetThr: 1.269 ± 0.031	1.687MetVal: 1.687 ± 0.033	0.209MetTrp: 0.209 ± 0.012	0.961MetTyr: 0.961 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	3.2AsnAla: 3.2 ± 0.05	0.79AsnCys: 0.79 ± 0.025	3.026AsnAsp: 3.026 ± 0.05	3.888AsnGlu: 3.888 ± 0.06	2.527AsnPhe: 2.527 ± 0.045	3.858AsnGly: 3.858 ± 0.056	0.817AsnHis: 0.817 ± 0.026	6.321AsnIle: 6.321 ± 0.084	5.35AsnLys: 5.35 ± 0.068	4.871AsnLeu: 4.871 ± 0.061	1.758AsnMet: 1.758 ± 0.034	3.847AsnAsn: 3.847 ± 0.06	1.933AsnPro: 1.933 ± 0.046	1.443AsnGln: 1.443 ± 0.03	2.22AsnArg: 2.22 ± 0.038	4.269AsnSer: 4.269 ± 0.069	2.991AsnThr: 2.991 ± 0.048	3.781AsnVal: 3.781 ± 0.048	0.532AsnTrp: 0.532 ± 0.02	2.873AsnTyr: 2.873 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	1.676ProAla: 1.676 ± 0.042	0.305ProCys: 0.305 ± 0.016	1.68ProAsp: 1.68 ± 0.039	2.212ProGlu: 2.212 ± 0.04	1.401ProPhe: 1.401 ± 0.034	1.667ProGly: 1.667 ± 0.039	0.527ProHis: 0.527 ± 0.019	2.546ProIle: 2.546 ± 0.045	2.145ProLys: 2.145 ± 0.042	2.446ProLeu: 2.446 ± 0.046	0.805ProMet: 0.805 ± 0.026	1.439ProAsn: 1.439 ± 0.038	0.622ProPro: 0.622 ± 0.022	0.871ProGln: 0.871 ± 0.028	0.861ProArg: 0.861 ± 0.03	1.787ProSer: 1.787 ± 0.037	1.46ProThr: 1.46 ± 0.031	2.17ProVal: 2.17 ± 0.044	0.308ProTrp: 0.308 ± 0.014	1.363ProTyr: 1.363 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	1.613GlnAla: 1.613 ± 0.035	0.295GlnCys: 0.295 ± 0.015	1.295GlnAsp: 1.295 ± 0.034	1.619GlnGlu: 1.619 ± 0.037	1.161GlnPhe: 1.161 ± 0.026	1.49GlnGly: 1.49 ± 0.036	0.443GlnHis: 0.443 ± 0.019	2.332GlnIle: 2.332 ± 0.042	2.405GlnLys: 2.405 ± 0.045	2.568GlnLeu: 2.568 ± 0.043	0.774GlnMet: 0.774 ± 0.027	1.709GlnAsn: 1.709 ± 0.034	0.668GlnPro: 0.668 ± 0.022	1.08GlnGln: 1.08 ± 0.034	1.069GlnArg: 1.069 ± 0.029	1.543GlnSer: 1.543 ± 0.033	1.214GlnThr: 1.214 ± 0.029	1.605GlnVal: 1.605 ± 0.037	0.286GlnTrp: 0.286 ± 0.015	1.387GlnTyr: 1.387 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	2.046ArgAla: 2.046 ± 0.038	0.441ArgCys: 0.441 ± 0.019	1.888ArgAsp: 1.888 ± 0.036	2.647ArgGlu: 2.647 ± 0.044	1.537ArgPhe: 1.537 ± 0.03	1.95ArgGly: 1.95 ± 0.042	0.544ArgHis: 0.544 ± 0.019	3.422ArgIle: 3.422 ± 0.051	3.397ArgLys: 3.397 ± 0.049	3.218ArgLeu: 3.218 ± 0.052	1.079ArgMet: 1.079 ± 0.027	2.39ArgAsn: 2.39 ± 0.038	0.916ArgPro: 0.916 ± 0.023	1.125ArgGln: 1.125 ± 0.031	1.692ArgArg: 1.692 ± 0.039	1.879ArgSer: 1.879 ± 0.04	1.827ArgThr: 1.827 ± 0.035	2.256ArgVal: 2.256 ± 0.038	0.326ArgTrp: 0.326 ± 0.017	1.693ArgTyr: 1.693 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	3.813SerAla: 3.813 ± 0.058	0.784SerCys: 0.784 ± 0.025	3.689SerAsp: 3.689 ± 0.057	4.138SerGlu: 4.138 ± 0.058	3.106SerPhe: 3.106 ± 0.047	4.513SerGly: 4.513 ± 0.06	0.953SerHis: 0.953 ± 0.027	6.565SerIle: 6.565 ± 0.076	5.725SerLys: 5.725 ± 0.065	5.719SerLeu: 5.719 ± 0.067	1.747SerMet: 1.747 ± 0.035	3.704SerAsn: 3.704 ± 0.061	1.711SerPro: 1.711 ± 0.037	1.738SerGln: 1.738 ± 0.038	2.416SerArg: 2.416 ± 0.044	5.051SerSer: 5.051 ± 0.082	3.389SerThr: 3.389 ± 0.054	4.348SerVal: 4.348 ± 0.055	0.55SerTrp: 0.55 ± 0.023	2.859SerTyr: 2.859 ± 0.053	0.0SerXaa: 0.0 ± 0.0
Thr	3.646ThrAla: 3.646 ± 0.063	0.539ThrCys: 0.539 ± 0.021	2.737ThrAsp: 2.737 ± 0.046	3.027ThrGlu: 3.027 ± 0.056	2.207ThrPhe: 2.207 ± 0.036	3.694ThrGly: 3.694 ± 0.06	0.8ThrHis: 0.8 ± 0.024	4.721ThrIle: 4.721 ± 0.066	3.605ThrLys: 3.605 ± 0.061	4.542ThrLeu: 4.542 ± 0.061	1.207ThrMet: 1.207 ± 0.029	2.57ThrAsn: 2.57 ± 0.046	1.758ThrPro: 1.758 ± 0.036	1.2ThrGln: 1.2 ± 0.034	1.639ThrArg: 1.639 ± 0.032	3.297ThrSer: 3.297 ± 0.056	2.736ThrThr: 2.736 ± 0.052	3.843ThrVal: 3.843 ± 0.061	0.416ThrTrp: 0.416 ± 0.018	2.128ThrTyr: 2.128 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	3.701ValAla: 3.701 ± 0.051	0.847ValCys: 0.847 ± 0.025	3.738ValAsp: 3.738 ± 0.047	4.021ValGlu: 4.021 ± 0.056	3.016ValPhe: 3.016 ± 0.062	3.773ValGly: 3.773 ± 0.059	0.937ValHis: 0.937 ± 0.023	6.057ValIle: 6.057 ± 0.063	5.438ValLys: 5.438 ± 0.068	5.897ValLeu: 5.897 ± 0.074	1.728ValMet: 1.728 ± 0.035	3.77ValAsn: 3.77 ± 0.051	2.093ValPro: 2.093 ± 0.037	1.573ValGln: 1.573 ± 0.036	2.125ValArg: 2.125 ± 0.037	4.56ValSer: 4.56 ± 0.061	3.547ValThr: 3.547 ± 0.061	4.301ValVal: 4.301 ± 0.067	0.508ValTrp: 0.508 ± 0.022	2.686ValTyr: 2.686 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	0.473TrpAla: 0.473 ± 0.02	0.133TrpCys: 0.133 ± 0.01	0.489TrpAsp: 0.489 ± 0.021	0.489TrpGlu: 0.489 ± 0.02	0.371TrpPhe: 0.371 ± 0.015	0.606TrpGly: 0.606 ± 0.022	0.174TrpHis: 0.174 ± 0.011	0.661TrpIle: 0.661 ± 0.022	0.73TrpLys: 0.73 ± 0.026	0.733TrpLeu: 0.733 ± 0.026	0.233TrpMet: 0.233 ± 0.014	0.623TrpAsn: 0.623 ± 0.022	0.217TrpPro: 0.217 ± 0.012	0.321TrpGln: 0.321 ± 0.015	0.315TrpArg: 0.315 ± 0.017	0.545TrpSer: 0.545 ± 0.023	0.416TrpThr: 0.416 ± 0.019	0.489TrpVal: 0.489 ± 0.021	0.131TrpTrp: 0.131 ± 0.011	0.359TrpTyr: 0.359 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.385TyrAla: 2.385 ± 0.044	0.619TyrCys: 0.619 ± 0.022	2.639TyrAsp: 2.639 ± 0.045	2.826TyrGlu: 2.826 ± 0.045	2.149TyrPhe: 2.149 ± 0.041	2.89TyrGly: 2.89 ± 0.051	0.688TyrHis: 0.688 ± 0.025	4.145TyrIle: 4.145 ± 0.06	3.774TyrLys: 3.774 ± 0.051	3.712TyrLeu: 3.712 ± 0.052	1.169TyrMet: 1.169 ± 0.027	2.752TyrAsn: 2.752 ± 0.052	1.375TyrPro: 1.375 ± 0.033	1.017TyrGln: 1.017 ± 0.029	1.794TyrArg: 1.794 ± 0.039	3.128TyrSer: 3.128 ± 0.05	2.2TyrThr: 2.2 ± 0.046	2.633TyrVal: 2.633 ± 0.046	0.407TyrTrp: 0.407 ± 0.015	2.205TyrTyr: 2.205 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4505 proteins (1424398 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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