Escherichia phage 1720a-02

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5M7Q0|S5M7Q0_9CAUD Uncharacterized protein OS=Escherichia phage 1720a-02 OX=1115653 PE=4 SV=1
MM1 pKa = 7.49TEE3 pKa = 3.97LSFFVHH9 pKa = 6.37PVDD12 pKa = 4.27MDD14 pKa = 3.81DD15 pKa = 5.07FSDD18 pKa = 3.6FRR20 pKa = 11.84KK21 pKa = 10.33SVFCPGAVACFIRR34 pKa = 11.84DD35 pKa = 3.81AIFAGYY41 pKa = 10.07SEE43 pKa = 5.61FSACC47 pKa = 5.25

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5MDR7|S5MDR7_9CAUD Regulatory protein OS=Escherichia phage 1720a-02 OX=1115653 PE=4 SV=1
MM1 pKa = 7.28IRR3 pKa = 11.84NIFKK7 pKa = 10.82RR8 pKa = 11.84FTNQTFRR15 pKa = 11.84CPRR18 pKa = 11.84PGQWYY23 pKa = 5.47TTPAGHH29 pKa = 6.23VLRR32 pKa = 11.84VSLVDD37 pKa = 3.87RR38 pKa = 11.84EE39 pKa = 4.3CQKK42 pKa = 10.88VICEE46 pKa = 4.01PLGRR50 pKa = 11.84NYY52 pKa = 9.75RR53 pKa = 11.84VSMPLIAFRR62 pKa = 11.84SGKK65 pKa = 8.95NMKK68 pKa = 9.95HH69 pKa = 6.22LGGAAA74 pKa = 3.13

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

14165

38

1155

208.3

23.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.756 ± 0.521

1.384 ± 0.164

5.711 ± 0.197

6.636 ± 0.232

3.572 ± 0.19

7.78 ± 0.435

1.807 ± 0.167

5.111 ± 0.261

5.344 ± 0.295

7.857 ± 0.258

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.718 ± 0.206

4.038 ± 0.168

4.391 ± 0.232

3.925 ± 0.281

6.608 ± 0.402

6.22 ± 0.218

5.824 ± 0.37

6.565 ± 0.26

1.814 ± 0.116

2.937 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski