Synechococcus phage S-WAM2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Cymopoleiavirus; Synechococcus virus SWAM2

Average proteome isoelectric point is 5.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8KSY2|A0A1D8KSY2_9CAUD Uncharacterized protein OS=Synechococcus phage S-WAM2 OX=1815522 GN=P29B0810_095 PE=4 SV=1
MM1 pKa = 7.39GFYY4 pKa = 10.6RR5 pKa = 11.84KK6 pKa = 7.73TLGVLSAASTDD17 pKa = 3.28KK18 pKa = 11.24DD19 pKa = 3.44IVDD22 pKa = 4.7FALTGTVFFTDD33 pKa = 3.14VNLRR37 pKa = 11.84KK38 pKa = 10.18YY39 pKa = 10.57FIADD43 pKa = 3.57DD44 pKa = 3.99NVVISSPGSNYY55 pKa = 10.37SVGDD59 pKa = 3.84YY60 pKa = 11.15VSVPLLGGTGSGAVADD76 pKa = 3.95IGVNDD81 pKa = 4.03WVGSITNAGSGYY93 pKa = 9.02TEE95 pKa = 3.89GTYY98 pKa = 10.28TSLYY102 pKa = 9.13PYY104 pKa = 9.91VISGAGNGDD113 pKa = 3.82ALVAISVNEE122 pKa = 3.88NGNISSFTVEE132 pKa = 3.95QYY134 pKa = 9.51GTGYY138 pKa = 7.89TQGTVLGLYY147 pKa = 10.05GATTSATTLSTVDD160 pKa = 3.97FNVTVSDD167 pKa = 3.64TSSILVNSAVTGTGIAEE184 pKa = 4.35GTTVASVLDD193 pKa = 3.92GTTVEE198 pKa = 4.35LSAYY202 pKa = 9.53PEE204 pKa = 3.85IDD206 pKa = 3.33GASTLTFTPSYY217 pKa = 10.81GGGNGFQFTISAINAVGTVTITDD240 pKa = 3.83GGNGYY245 pKa = 10.12AAGDD249 pKa = 3.94TLTVADD255 pKa = 4.94ASLSVPIEE263 pKa = 3.99YY264 pKa = 10.2VVSDD268 pKa = 4.15LSVQRR273 pKa = 11.84LTFVEE278 pKa = 4.92TIPVGTFSIGGTLEE292 pKa = 3.63ISTLDD297 pKa = 3.21GTQYY301 pKa = 11.14NIVEE305 pKa = 4.37IEE307 pKa = 4.12TSGGNITSVVLRR319 pKa = 11.84DD320 pKa = 3.59SFFGATEE327 pKa = 3.75TLAYY331 pKa = 9.96NGSATPIYY339 pKa = 9.16TVDD342 pKa = 3.14VATSEE347 pKa = 3.99FRR349 pKa = 11.84AGIDD353 pKa = 2.98IGDD356 pKa = 3.89GNGVQFTPNLTLYY369 pKa = 10.72EE370 pKa = 4.32DD371 pKa = 3.27ATYY374 pKa = 10.89EE375 pKa = 4.04FTYY378 pKa = 10.65SGSNTFALSQTQDD391 pKa = 3.06GPGEE395 pKa = 4.15YY396 pKa = 10.37APSTVVRR403 pKa = 11.84DD404 pKa = 4.01TEE406 pKa = 4.45NNTLTVSVTDD416 pKa = 4.31SYY418 pKa = 11.72PSTLYY423 pKa = 10.88YY424 pKa = 10.73FSTDD428 pKa = 2.85NPNYY432 pKa = 9.92GGSSSITIDD441 pKa = 3.72PNNPSPPGTGFQLLVNTVTILDD463 pKa = 4.54SIALDD468 pKa = 4.09IIDD471 pKa = 4.61GSVTALDD478 pKa = 4.21VITTNLTATTGTVSTLTSTSGDD500 pKa = 2.86ITTLKK505 pKa = 9.03ITSLSDD511 pKa = 3.0KK512 pKa = 10.73GSGIAVTTGGSTNITLTPGNSLNVGGALSIEE543 pKa = 4.47SATGNLTTSGVLKK556 pKa = 10.05STGSFNSNDD565 pKa = 3.17KK566 pKa = 11.11LLISEE571 pKa = 4.62NSISSLGTSDD581 pKa = 4.8VVLAPIANTNAKK593 pKa = 10.09VSGTMSLIIPSGDD606 pKa = 2.61ISQRR610 pKa = 11.84PQGAKK615 pKa = 10.22AEE617 pKa = 4.3SGSIRR622 pKa = 11.84FNTEE626 pKa = 3.29TQQYY630 pKa = 9.2EE631 pKa = 5.01GYY633 pKa = 10.54NGIAAAWSSLGGVRR647 pKa = 11.84DD648 pKa = 3.63VDD650 pKa = 3.62GNTYY654 pKa = 10.3ILAEE658 pKa = 4.45EE659 pKa = 4.09FTGANDD665 pKa = 3.31NTFWFYY671 pKa = 11.73NGGTNSLTISQSEE684 pKa = 4.32INLKK688 pKa = 10.03SANKK692 pKa = 9.66FKK694 pKa = 10.56STDD697 pKa = 3.39VTSYY701 pKa = 10.51TPWAGNTYY709 pKa = 10.21YY710 pKa = 11.38GLGEE714 pKa = 5.27LLYY717 pKa = 10.55QDD719 pKa = 4.16LNVYY723 pKa = 9.12EE724 pKa = 4.25VTVAGLSDD732 pKa = 4.44TIAPTHH738 pKa = 5.46EE739 pKa = 4.45TGAVTANGGTPTIAGAIAAFTVSTPSGNNAVGTYY773 pKa = 10.03SYY775 pKa = 11.58DD776 pKa = 3.42DD777 pKa = 3.95TYY779 pKa = 11.9GSAQFEE785 pKa = 4.99IIFQLGTVEE794 pKa = 5.16VNLLSQGSGGYY805 pKa = 7.8STSGGGVDD813 pKa = 4.1NVITILGSLIGGTDD827 pKa = 3.49GVDD830 pKa = 3.47DD831 pKa = 4.03LTITITEE838 pKa = 3.89IAVALEE844 pKa = 3.99LTWYY848 pKa = 7.53GTTAGNIEE856 pKa = 4.2FEE858 pKa = 4.72DD859 pKa = 3.68VNEE862 pKa = 4.46VIFTDD867 pKa = 3.99STIHH871 pKa = 6.75ASNTISGRR879 pKa = 11.84NLLITGGEE887 pKa = 4.0IEE889 pKa = 4.45GDD891 pKa = 3.24GDD893 pKa = 4.3LSIKK897 pKa = 10.47IPDD900 pKa = 3.75GSNLVVTATGSLAVPVGDD918 pKa = 3.29NLQRR922 pKa = 11.84GTPVAGSIRR931 pKa = 11.84YY932 pKa = 9.14NNEE935 pKa = 2.92INQYY939 pKa = 9.94EE940 pKa = 5.04GYY942 pKa = 10.93NSAASNWSSLGGVRR956 pKa = 11.84DD957 pKa = 3.63VDD959 pKa = 3.61GNTYY963 pKa = 10.37IIPEE967 pKa = 4.15SSAGANEE974 pKa = 4.39NILYY978 pKa = 9.77FYY980 pKa = 11.37NNDD983 pKa = 3.69DD984 pKa = 3.37NTLQVTQSQVLFGTIDD1000 pKa = 4.01SISSTSNALNINVEE1014 pKa = 4.05NVTFNNLHH1022 pKa = 6.2SGIDD1026 pKa = 3.59VSDD1029 pKa = 3.44ATTTLVYY1036 pKa = 10.83SSVNNLDD1043 pKa = 3.91FGLSTGLTIDD1053 pKa = 3.36TVLRR1057 pKa = 11.84LTDD1060 pKa = 3.51AGEE1063 pKa = 3.83IFLNKK1068 pKa = 10.33GYY1070 pKa = 8.6GTGVFEE1076 pKa = 4.23GVKK1079 pKa = 10.6FIDD1082 pKa = 3.71AFLKK1086 pKa = 10.09TFEE1089 pKa = 5.14LDD1091 pKa = 3.34DD1092 pKa = 3.98VQIKK1096 pKa = 9.58TDD1098 pKa = 3.94DD1099 pKa = 3.59VVLTKK1104 pKa = 10.16GTTDD1108 pKa = 2.78TGTVVLYY1115 pKa = 10.76DD1116 pKa = 3.55PVEE1119 pKa = 4.28AKK1121 pKa = 9.9SSKK1124 pKa = 10.83VIVTAYY1130 pKa = 7.97NTTTEE1135 pKa = 4.16DD1136 pKa = 3.31VHH1138 pKa = 7.28TEE1140 pKa = 4.13EE1141 pKa = 4.95ISVIVKK1147 pKa = 10.58GSDD1150 pKa = 3.11LYY1152 pKa = 10.08TVEE1155 pKa = 4.16YY1156 pKa = 8.53GTNKK1160 pKa = 6.72TTNLFAAVVDD1170 pKa = 4.92LNATGKK1176 pKa = 9.85VRR1178 pKa = 11.84LSLALDD1184 pKa = 3.33SGIATGEE1191 pKa = 3.96IVNITVVRR1199 pKa = 11.84TNVKK1203 pKa = 10.03KK1204 pKa = 10.88

Molecular weight: 125.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8KSU6|A0A1D8KSU6_9CAUD Carbon metabolic regulator OS=Synechococcus phage S-WAM2 OX=1815522 GN=P29B0810_011 PE=4 SV=1
MM1 pKa = 8.12RR2 pKa = 11.84SGQPDD7 pKa = 3.65PSSAGNHH14 pKa = 5.48SASKK18 pKa = 10.47FSRR21 pKa = 11.84KK22 pKa = 7.49QLCSNLFSQLPLLHH36 pKa = 6.47PLWRR40 pKa = 11.84PLLWQVPMM48 pKa = 4.91

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

229

0

229

57339

37

4759

250.4

27.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.321 ± 0.269

0.869 ± 0.079

6.639 ± 0.167

6.17 ± 0.285

4.301 ± 0.113

7.862 ± 0.369

1.428 ± 0.111

6.219 ± 0.193

5.467 ± 0.333

7.135 ± 0.157

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.913 ± 0.198

5.972 ± 0.178

3.938 ± 0.111

3.697 ± 0.105

3.929 ± 0.111

7.021 ± 0.274

7.728 ± 0.333

6.807 ± 0.177

1.151 ± 0.086

4.432 ± 0.111
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski