Amino acid dipepetide frequency for Synechococcus phage S-WAM2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.523AlaAla: 6.523 ± 0.686
0.645AlaCys: 0.645 ± 0.104
4.883AlaAsp: 4.883 ± 0.388
3.959AlaGlu: 3.959 ± 0.295
2.965AlaPhe: 2.965 ± 0.22
6.941AlaGly: 6.941 ± 0.559
0.82AlaHis: 0.82 ± 0.12
4.186AlaIle: 4.186 ± 0.326
3.854AlaLys: 3.854 ± 0.37
4.761AlaLeu: 4.761 ± 0.337
1.203AlaMet: 1.203 ± 0.193
4.186AlaAsn: 4.186 ± 0.354
3.104AlaPro: 3.104 ± 0.207
2.581AlaGln: 2.581 ± 0.212
2.511AlaArg: 2.511 ± 0.212
5.476AlaSer: 5.476 ± 0.379
6.383AlaThr: 6.383 ± 0.451
4.744AlaVal: 4.744 ± 0.317
0.767AlaTrp: 0.767 ± 0.145
2.721AlaTyr: 2.721 ± 0.216
0.0AlaXaa: 0.0 ± 0.0
Cys
0.767CysAla: 0.767 ± 0.144
0.157CysCys: 0.157 ± 0.049
0.68CysAsp: 0.68 ± 0.11
0.541CysGlu: 0.541 ± 0.104
0.349CysPhe: 0.349 ± 0.08
0.61CysGly: 0.61 ± 0.122
0.227CysHis: 0.227 ± 0.066
0.558CysIle: 0.558 ± 0.113
0.576CysLys: 0.576 ± 0.109
0.541CysLeu: 0.541 ± 0.111
0.174CysMet: 0.174 ± 0.058
0.453CysAsn: 0.453 ± 0.083
0.331CysPro: 0.331 ± 0.091
0.436CysGln: 0.436 ± 0.078
0.366CysArg: 0.366 ± 0.087
0.506CysSer: 0.506 ± 0.103
0.331CysThr: 0.331 ± 0.086
0.68CysVal: 0.68 ± 0.138
0.087CysTrp: 0.087 ± 0.04
0.314CysTyr: 0.314 ± 0.07
0.0CysXaa: 0.0 ± 0.0
Asp
5.389AspAla: 5.389 ± 0.482
0.541AspCys: 0.541 ± 0.1
4.744AspAsp: 4.744 ± 0.347
3.942AspGlu: 3.942 ± 0.288
3.052AspPhe: 3.052 ± 0.245
5.127AspGly: 5.127 ± 0.297
1.169AspHis: 1.169 ± 0.143
4.221AspIle: 4.221 ± 0.287
3.122AspLys: 3.122 ± 0.296
4.761AspLeu: 4.761 ± 0.294
1.378AspMet: 1.378 ± 0.183
3.907AspAsn: 3.907 ± 0.265
3.314AspPro: 3.314 ± 0.35
2.11AspGln: 2.11 ± 0.207
2.616AspArg: 2.616 ± 0.23
3.383AspSer: 3.383 ± 0.251
4.674AspThr: 4.674 ± 0.278
4.552AspVal: 4.552 ± 0.292
1.099AspTrp: 1.099 ± 0.175
3.296AspTyr: 3.296 ± 0.276
0.0AspXaa: 0.0 ± 0.0
Glu
3.889GluAla: 3.889 ± 0.254
0.593GluCys: 0.593 ± 0.106
3.732GluAsp: 3.732 ± 0.311
5.372GluGlu: 5.372 ± 0.496
3.0GluPhe: 3.0 ± 0.275
4.186GluGly: 4.186 ± 0.293
0.994GluHis: 0.994 ± 0.163
4.168GluIle: 4.168 ± 0.27
3.907GluLys: 3.907 ± 0.405
4.622GluLeu: 4.622 ± 0.364
1.622GluMet: 1.622 ± 0.215
3.296GluAsn: 3.296 ± 0.251
2.145GluPro: 2.145 ± 0.227
2.145GluGln: 2.145 ± 0.244
2.529GluArg: 2.529 ± 0.262
2.79GluSer: 2.79 ± 0.223
3.907GluThr: 3.907 ± 0.214
4.848GluVal: 4.848 ± 0.336
1.134GluTrp: 1.134 ± 0.173
2.825GluTyr: 2.825 ± 0.249
0.0GluXaa: 0.0 ± 0.0
Phe
3.157PheAla: 3.157 ± 0.251
0.453PheCys: 0.453 ± 0.088
3.07PheAsp: 3.07 ± 0.21
2.947PheGlu: 2.947 ± 0.226
1.744PhePhe: 1.744 ± 0.182
3.104PheGly: 3.104 ± 0.214
0.715PheHis: 0.715 ± 0.142
2.093PheIle: 2.093 ± 0.254
2.354PheLys: 2.354 ± 0.23
2.982PheLeu: 2.982 ± 0.26
0.907PheMet: 0.907 ± 0.151
2.668PheAsn: 2.668 ± 0.287
1.413PhePro: 1.413 ± 0.189
1.517PheGln: 1.517 ± 0.177
1.727PheArg: 1.727 ± 0.183
3.296PheSer: 3.296 ± 0.242
3.506PheThr: 3.506 ± 0.248
3.104PheVal: 3.104 ± 0.223
0.366PheTrp: 0.366 ± 0.079
1.884PheTyr: 1.884 ± 0.189
0.0PheXaa: 0.0 ± 0.0
Gly
6.279GlyAla: 6.279 ± 0.464
0.558GlyCys: 0.558 ± 0.097
5.11GlyAsp: 5.11 ± 0.521
4.221GlyGlu: 4.221 ± 0.258
2.738GlyPhe: 2.738 ± 0.206
8.337GlyGly: 8.337 ± 1.352
0.872GlyHis: 0.872 ± 0.129
3.819GlyIle: 3.819 ± 0.256
3.593GlyLys: 3.593 ± 0.311
4.587GlyLeu: 4.587 ± 0.326
1.395GlyMet: 1.395 ± 0.197
5.633GlyAsn: 5.633 ± 0.513
1.814GlyPro: 1.814 ± 0.172
2.773GlyGln: 2.773 ± 0.241
2.651GlyArg: 2.651 ± 0.201
7.342GlySer: 7.342 ± 0.601
7.046GlyThr: 7.046 ± 0.555
5.511GlyVal: 5.511 ± 0.336
0.907GlyTrp: 0.907 ± 0.144
3.436GlyTyr: 3.436 ± 0.321
0.0GlyXaa: 0.0 ± 0.0
His
0.872HisAla: 0.872 ± 0.156
0.192HisCys: 0.192 ± 0.067
0.959HisAsp: 0.959 ± 0.145
0.994HisGlu: 0.994 ± 0.178
0.628HisPhe: 0.628 ± 0.108
1.012HisGly: 1.012 ± 0.154
0.279HisHis: 0.279 ± 0.082
0.872HisIle: 0.872 ± 0.148
0.68HisLys: 0.68 ± 0.136
0.942HisLeu: 0.942 ± 0.154
0.349HisMet: 0.349 ± 0.095
0.732HisAsn: 0.732 ± 0.117
0.889HisPro: 0.889 ± 0.152
0.349HisGln: 0.349 ± 0.073
0.75HisArg: 0.75 ± 0.121
1.099HisSer: 1.099 ± 0.174
0.977HisThr: 0.977 ± 0.142
0.855HisVal: 0.855 ± 0.146
0.105HisTrp: 0.105 ± 0.046
0.75HisTyr: 0.75 ± 0.134
0.0HisXaa: 0.0 ± 0.0
Ile
4.517IleAla: 4.517 ± 0.283
0.576IleCys: 0.576 ± 0.102
4.988IleAsp: 4.988 ± 0.394
3.785IleGlu: 3.785 ± 0.286
2.407IlePhe: 2.407 ± 0.216
4.482IleGly: 4.482 ± 0.396
0.732IleHis: 0.732 ± 0.099
3.628IleIle: 3.628 ± 0.296
3.558IleLys: 3.558 ± 0.287
4.343IleLeu: 4.343 ± 0.32
0.924IleMet: 0.924 ± 0.147
3.61IleAsn: 3.61 ± 0.308
2.668IlePro: 2.668 ± 0.209
2.634IleGln: 2.634 ± 0.27
2.494IleArg: 2.494 ± 0.218
4.639IleSer: 4.639 ± 0.354
5.546IleThr: 5.546 ± 0.388
4.133IleVal: 4.133 ± 0.43
0.645IleTrp: 0.645 ± 0.119
1.884IleTyr: 1.884 ± 0.194
0.0IleXaa: 0.0 ± 0.0
Lys
3.017LysAla: 3.017 ± 0.221
0.732LysCys: 0.732 ± 0.124
3.331LysAsp: 3.331 ± 0.253
3.889LysGlu: 3.889 ± 0.389
2.581LysPhe: 2.581 ± 0.251
2.93LysGly: 2.93 ± 0.334
0.75LysHis: 0.75 ± 0.152
4.168LysIle: 4.168 ± 0.335
4.133LysLys: 4.133 ± 0.424
4.325LysLeu: 4.325 ± 0.293
1.674LysMet: 1.674 ± 0.251
2.825LysAsn: 2.825 ± 0.235
1.622LysPro: 1.622 ± 0.184
2.25LysGln: 2.25 ± 0.224
2.389LysArg: 2.389 ± 0.214
3.157LysSer: 3.157 ± 0.245
3.924LysThr: 3.924 ± 0.306
3.453LysVal: 3.453 ± 0.26
0.523LysTrp: 0.523 ± 0.119
3.0LysTyr: 3.0 ± 0.245
0.0LysXaa: 0.0 ± 0.0
Leu
4.552LeuAla: 4.552 ± 0.311
0.645LeuCys: 0.645 ± 0.122
5.389LeuAsp: 5.389 ± 0.3
4.657LeuGlu: 4.657 ± 0.319
2.721LeuPhe: 2.721 ± 0.245
4.552LeuGly: 4.552 ± 0.391
1.325LeuHis: 1.325 ± 0.224
4.221LeuIle: 4.221 ± 0.281
4.255LeuLys: 4.255 ± 0.32
4.848LeuLeu: 4.848 ± 0.415
1.308LeuMet: 1.308 ± 0.183
4.36LeuAsn: 4.36 ± 0.259
3.157LeuPro: 3.157 ± 0.241
3.052LeuGln: 3.052 ± 0.237
3.017LeuArg: 3.017 ± 0.218
4.866LeuSer: 4.866 ± 0.333
5.93LeuThr: 5.93 ± 0.346
4.517LeuVal: 4.517 ± 0.278
0.732LeuTrp: 0.732 ± 0.114
3.244LeuTyr: 3.244 ± 0.224
0.0LeuXaa: 0.0 ± 0.0
Met
1.395MetAla: 1.395 ± 0.193
0.174MetCys: 0.174 ± 0.072
1.012MetAsp: 1.012 ± 0.162
0.942MetGlu: 0.942 ± 0.158
0.977MetPhe: 0.977 ± 0.143
1.221MetGly: 1.221 ± 0.166
0.296MetHis: 0.296 ± 0.102
1.064MetIle: 1.064 ± 0.183
1.884MetLys: 1.884 ± 0.257
1.587MetLeu: 1.587 ± 0.231
0.61MetMet: 0.61 ± 0.127
0.994MetAsn: 0.994 ± 0.185
0.942MetPro: 0.942 ± 0.154
0.645MetGln: 0.645 ± 0.123
0.698MetArg: 0.698 ± 0.096
1.186MetSer: 1.186 ± 0.172
1.308MetThr: 1.308 ± 0.206
1.169MetVal: 1.169 ± 0.132
0.296MetTrp: 0.296 ± 0.072
0.732MetTyr: 0.732 ± 0.118
0.0MetXaa: 0.0 ± 0.0
Asn
3.959AsnAla: 3.959 ± 0.384
0.401AsnCys: 0.401 ± 0.096
3.279AsnAsp: 3.279 ± 0.226
3.261AsnGlu: 3.261 ± 0.246
2.79AsnPhe: 2.79 ± 0.24
4.779AsnGly: 4.779 ± 0.432
1.029AsnHis: 1.029 ± 0.151
4.133AsnIle: 4.133 ± 0.262
2.651AsnLys: 2.651 ± 0.221
4.726AsnLeu: 4.726 ± 0.268
0.942AsnMet: 0.942 ± 0.126
3.959AsnAsn: 3.959 ± 0.31
2.913AsnPro: 2.913 ± 0.232
2.18AsnGln: 2.18 ± 0.22
2.494AsnArg: 2.494 ± 0.246
4.604AsnSer: 4.604 ± 0.323
4.482AsnThr: 4.482 ± 0.32
4.046AsnVal: 4.046 ± 0.253
0.732AsnTrp: 0.732 ± 0.107
2.354AsnTyr: 2.354 ± 0.186
0.0AsnXaa: 0.0 ± 0.0
Pro
3.0ProAla: 3.0 ± 0.235
0.157ProCys: 0.157 ± 0.052
2.738ProAsp: 2.738 ± 0.275
2.703ProGlu: 2.703 ± 0.266
1.639ProPhe: 1.639 ± 0.198
3.157ProGly: 3.157 ± 0.285
0.419ProHis: 0.419 ± 0.081
2.145ProIle: 2.145 ± 0.179
1.779ProLys: 1.779 ± 0.222
2.616ProLeu: 2.616 ± 0.195
0.68ProMet: 0.68 ± 0.131
2.32ProAsn: 2.32 ± 0.184
1.57ProPro: 1.57 ± 0.16
1.692ProGln: 1.692 ± 0.195
1.465ProArg: 1.465 ± 0.2
2.965ProSer: 2.965 ± 0.27
3.54ProThr: 3.54 ± 0.25
2.843ProVal: 2.843 ± 0.197
0.436ProTrp: 0.436 ± 0.115
1.517ProTyr: 1.517 ± 0.158
0.0ProXaa: 0.0 ± 0.0
Gln
2.407GlnAla: 2.407 ± 0.285
0.349GlnCys: 0.349 ± 0.093
2.145GlnAsp: 2.145 ± 0.18
2.302GlnGlu: 2.302 ± 0.25
1.849GlnPhe: 1.849 ± 0.166
2.267GlnGly: 2.267 ± 0.204
0.628GlnHis: 0.628 ± 0.118
2.302GlnIle: 2.302 ± 0.247
2.32GlnLys: 2.32 ± 0.231
3.157GlnLeu: 3.157 ± 0.279
0.732GlnMet: 0.732 ± 0.138
2.075GlnAsn: 2.075 ± 0.163
1.186GlnPro: 1.186 ± 0.133
1.5GlnGln: 1.5 ± 0.192
1.5GlnArg: 1.5 ± 0.183
2.651GlnSer: 2.651 ± 0.207
2.703GlnThr: 2.703 ± 0.286
2.389GlnVal: 2.389 ± 0.222
0.663GlnTrp: 0.663 ± 0.115
1.849GlnTyr: 1.849 ± 0.163
0.0GlnXaa: 0.0 ± 0.0
Arg
2.965ArgAla: 2.965 ± 0.234
0.314ArgCys: 0.314 ± 0.081
2.424ArgAsp: 2.424 ± 0.219
2.459ArgGlu: 2.459 ± 0.244
1.796ArgPhe: 1.796 ± 0.176
2.546ArgGly: 2.546 ± 0.209
0.593ArgHis: 0.593 ± 0.117
2.634ArgIle: 2.634 ± 0.21
2.407ArgLys: 2.407 ± 0.258
3.226ArgLeu: 3.226 ± 0.226
0.907ArgMet: 0.907 ± 0.148
2.197ArgAsn: 2.197 ± 0.189
1.186ArgPro: 1.186 ± 0.137
1.587ArgGln: 1.587 ± 0.208
2.32ArgArg: 2.32 ± 0.256
2.337ArgSer: 2.337 ± 0.208
2.128ArgThr: 2.128 ± 0.2
2.477ArgVal: 2.477 ± 0.236
0.506ArgTrp: 0.506 ± 0.085
2.285ArgTyr: 2.285 ± 0.207
0.0ArgXaa: 0.0 ± 0.0
Ser
5.703SerAla: 5.703 ± 0.35
0.488SerCys: 0.488 ± 0.1
4.099SerAsp: 4.099 ± 0.248
3.558SerGlu: 3.558 ± 0.301
2.913SerPhe: 2.913 ± 0.275
7.97SerGly: 7.97 ± 0.733
0.889SerHis: 0.889 ± 0.136
5.145SerIle: 5.145 ± 0.285
3.523SerLys: 3.523 ± 0.281
4.883SerLeu: 4.883 ± 0.311
1.029SerMet: 1.029 ± 0.201
4.29SerAsn: 4.29 ± 0.332
2.354SerPro: 2.354 ± 0.189
2.285SerGln: 2.285 ± 0.185
2.407SerArg: 2.407 ± 0.203
5.441SerSer: 5.441 ± 0.533
5.058SerThr: 5.058 ± 0.358
4.535SerVal: 4.535 ± 0.345
0.645SerTrp: 0.645 ± 0.129
3.0SerTyr: 3.0 ± 0.254
0.0SerXaa: 0.0 ± 0.0
Thr
6.122ThrAla: 6.122 ± 0.409
0.506ThrCys: 0.506 ± 0.106
4.709ThrAsp: 4.709 ± 0.361
4.325ThrGlu: 4.325 ± 0.275
3.401ThrPhe: 3.401 ± 0.248
7.028ThrGly: 7.028 ± 0.564
0.924ThrHis: 0.924 ± 0.146
5.337ThrIle: 5.337 ± 0.455
3.192ThrLys: 3.192 ± 0.292
5.476ThrLeu: 5.476 ± 0.353
0.959ThrMet: 0.959 ± 0.124
4.604ThrAsn: 4.604 ± 0.422
3.889ThrPro: 3.889 ± 0.294
2.808ThrGln: 2.808 ± 0.198
2.093ThrArg: 2.093 ± 0.197
5.773ThrSer: 5.773 ± 0.494
6.156ThrThr: 6.156 ± 0.431
5.424ThrVal: 5.424 ± 0.505
0.802ThrTrp: 0.802 ± 0.135
3.75ThrTyr: 3.75 ± 0.296
0.0ThrXaa: 0.0 ± 0.0
Val
5.162ValAla: 5.162 ± 0.307
0.593ValCys: 0.593 ± 0.116
5.093ValAsp: 5.093 ± 0.342
4.325ValGlu: 4.325 ± 0.342
2.965ValPhe: 2.965 ± 0.18
4.691ValGly: 4.691 ± 0.347
0.75ValHis: 0.75 ± 0.136
3.942ValIle: 3.942 ± 0.269
3.994ValLys: 3.994 ± 0.271
4.726ValLeu: 4.726 ± 0.226
1.081ValMet: 1.081 ± 0.146
3.854ValAsn: 3.854 ± 0.206
2.756ValPro: 2.756 ± 0.262
2.18ValGln: 2.18 ± 0.215
2.93ValArg: 2.93 ± 0.293
5.197ValSer: 5.197 ± 0.402
5.651ValThr: 5.651 ± 0.436
4.918ValVal: 4.918 ± 0.342
0.506ValTrp: 0.506 ± 0.117
2.756ValTyr: 2.756 ± 0.252
0.0ValXaa: 0.0 ± 0.0
Trp
0.837TrpAla: 0.837 ± 0.126
0.07TrpCys: 0.07 ± 0.036
0.959TrpAsp: 0.959 ± 0.124
0.802TrpGlu: 0.802 ± 0.132
0.419TrpPhe: 0.419 ± 0.081
0.837TrpGly: 0.837 ± 0.155
0.296TrpHis: 0.296 ± 0.079
0.541TrpIle: 0.541 ± 0.086
0.715TrpLys: 0.715 ± 0.123
0.785TrpLeu: 0.785 ± 0.129
0.349TrpMet: 0.349 ± 0.091
0.698TrpAsn: 0.698 ± 0.099
0.227TrpPro: 0.227 ± 0.072
0.506TrpGln: 0.506 ± 0.12
0.471TrpArg: 0.471 ± 0.074
0.82TrpSer: 0.82 ± 0.133
0.732TrpThr: 0.732 ± 0.116
0.75TrpVal: 0.75 ± 0.103
0.14TrpTrp: 0.14 ± 0.047
0.541TrpTyr: 0.541 ± 0.105
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.703TyrAla: 2.703 ± 0.236
0.541TyrCys: 0.541 ± 0.114
3.052TyrAsp: 3.052 ± 0.268
2.721TyrGlu: 2.721 ± 0.27
2.075TyrPhe: 2.075 ± 0.186
2.843TyrGly: 2.843 ± 0.28
0.576TyrHis: 0.576 ± 0.109
3.017TyrIle: 3.017 ± 0.301
2.18TyrLys: 2.18 ± 0.211
3.453TyrLeu: 3.453 ± 0.217
0.889TyrMet: 0.889 ± 0.149
3.052TyrAsn: 3.052 ± 0.262
1.901TyrPro: 1.901 ± 0.179
1.744TyrGln: 1.744 ± 0.181
1.866TyrArg: 1.866 ± 0.198
2.686TyrSer: 2.686 ± 0.191
3.296TyrThr: 3.296 ± 0.27
3.122TyrVal: 3.122 ± 0.261
0.419TyrTrp: 0.419 ± 0.089
2.18TyrTyr: 2.18 ± 0.272
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 229 proteins (57339 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski