Klebsiella phage ST846-OXA48phi9.2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482ILZ6|A0A482ILZ6_9CAUD Uncharacterized protein OS=Klebsiella phage ST846-OXA48phi9.2 OX=2510489 PE=4 SV=1
MM1 pKa = 7.68LYY3 pKa = 10.4VSSPFEE9 pKa = 4.06AKK11 pKa = 9.53VYY13 pKa = 10.22RR14 pKa = 11.84YY15 pKa = 7.64FVPEE19 pKa = 5.17DD20 pKa = 3.89YY21 pKa = 11.3SPDD24 pKa = 3.48QQFTSFDD31 pKa = 3.67VLLSTLVV38 pKa = 3.14

Molecular weight: 4.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482ILV9|A0A482ILV9_9CAUD DNA polymerase III theta subunit OS=Klebsiella phage ST846-OXA48phi9.2 OX=2510489 PE=4 SV=1
MM1 pKa = 7.42ARR3 pKa = 11.84AFSAVEE9 pKa = 3.27RR10 pKa = 11.84RR11 pKa = 11.84EE12 pKa = 3.7YY13 pKa = 10.04VRR15 pKa = 11.84AVIRR19 pKa = 11.84ITRR22 pKa = 11.84HH23 pKa = 4.0QGRR26 pKa = 11.84LTTAEE31 pKa = 3.94AMKK34 pKa = 10.83KK35 pKa = 10.16LGLSRR40 pKa = 11.84ATVQRR45 pKa = 11.84YY46 pKa = 7.82FSEE49 pKa = 5.16AEE51 pKa = 3.76ATGEE55 pKa = 4.31VVRR58 pKa = 11.84HH59 pKa = 5.21GRR61 pKa = 11.84LGLFRR66 pKa = 11.84DD67 pKa = 3.57QRR69 pKa = 11.84AVIDD73 pKa = 3.78FDD75 pKa = 3.63MKK77 pKa = 10.91RR78 pKa = 11.84FGLVPKK84 pKa = 10.23VAVGMNYY91 pKa = 10.41SLLGSSVFQRR101 pKa = 11.84VLDD104 pKa = 3.83VQEE107 pKa = 5.21AIHH110 pKa = 6.31GG111 pKa = 4.0

Molecular weight: 12.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

17045

37

1032

187.3

20.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.786 ± 0.318

1.25 ± 0.126

6.125 ± 0.175

6.189 ± 0.289

3.538 ± 0.172

7.287 ± 0.286

1.678 ± 0.132

5.714 ± 0.205

5.339 ± 0.302

8.9 ± 0.385

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.505 ± 0.132

3.884 ± 0.176

4.248 ± 0.174

4.124 ± 0.242

6.066 ± 0.271

6.096 ± 0.22

6.019 ± 0.333

6.612 ± 0.255

1.561 ± 0.108

3.08 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski