Amino acid dipepetide frequency for Phycisphaerae bacterium RAS1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.046AlaAla: 20.046 ± 0.191	1.689AlaCys: 1.689 ± 0.044	7.386AlaAsp: 7.386 ± 0.071	6.885AlaGlu: 6.885 ± 0.081	3.829AlaPhe: 3.829 ± 0.056	11.405AlaGly: 11.405 ± 0.102	1.991AlaHis: 1.991 ± 0.036	5.197AlaIle: 5.197 ± 0.057	2.988AlaLys: 2.988 ± 0.062	12.05AlaLeu: 12.05 ± 0.139	2.555AlaMet: 2.555 ± 0.044	2.793AlaAsn: 2.793 ± 0.053	5.967AlaPro: 5.967 ± 0.063	3.648AlaGln: 3.648 ± 0.061	8.933AlaArg: 8.933 ± 0.099	6.194AlaSer: 6.194 ± 0.072	6.29AlaThr: 6.29 ± 0.073	8.483AlaVal: 8.483 ± 0.086	1.797AlaTrp: 1.797 ± 0.035	2.584AlaTyr: 2.584 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	1.344CysAla: 1.344 ± 0.039	0.324CysCys: 0.324 ± 0.02	1.074CysAsp: 1.074 ± 0.035	0.746CysGlu: 0.746 ± 0.022	0.485CysPhe: 0.485 ± 0.02	1.525CysGly: 1.525 ± 0.048	0.37CysHis: 0.37 ± 0.029	0.545CysIle: 0.545 ± 0.021	0.315CysLys: 0.315 ± 0.015	1.106CysLeu: 1.106 ± 0.033	0.199CysMet: 0.199 ± 0.012	0.456CysAsn: 0.456 ± 0.022	0.878CysPro: 0.878 ± 0.031	0.356CysGln: 0.356 ± 0.014	0.983CysArg: 0.983 ± 0.03	0.692CysSer: 0.692 ± 0.025	0.726CysThr: 0.726 ± 0.023	1.066CysVal: 1.066 ± 0.036	0.204CysTrp: 0.204 ± 0.012	0.333CysTyr: 0.333 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	7.93AspAla: 7.93 ± 0.08	0.791AspCys: 0.791 ± 0.035	3.802AspAsp: 3.802 ± 0.062	3.908AspGlu: 3.908 ± 0.059	2.172AspPhe: 2.172 ± 0.04	6.27AspGly: 6.27 ± 0.101	1.069AspHis: 1.069 ± 0.029	2.71AspIle: 2.71 ± 0.047	1.458AspLys: 1.458 ± 0.038	5.315AspLeu: 5.315 ± 0.06	1.002AspMet: 1.002 ± 0.027	1.511AspAsn: 1.511 ± 0.043	3.823AspPro: 3.823 ± 0.05	1.689AspGln: 1.689 ± 0.033	4.101AspArg: 4.101 ± 0.056	2.708AspSer: 2.708 ± 0.044	2.4AspThr: 2.4 ± 0.049	4.769AspVal: 4.769 ± 0.05	0.956AspTrp: 0.956 ± 0.026	1.412AspTyr: 1.412 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	6.162GluAla: 6.162 ± 0.092	0.662GluCys: 0.662 ± 0.024	2.319GluAsp: 2.319 ± 0.044	2.621GluGlu: 2.621 ± 0.056	2.158GluPhe: 2.158 ± 0.038	3.284GluGly: 3.284 ± 0.049	1.348GluHis: 1.348 ± 0.033	3.047GluIle: 3.047 ± 0.051	2.121GluLys: 2.121 ± 0.049	6.369GluLeu: 6.369 ± 0.071	1.333GluMet: 1.333 ± 0.028	1.536GluAsn: 1.536 ± 0.038	2.995GluPro: 2.995 ± 0.046	2.49GluGln: 2.49 ± 0.043	4.915GluArg: 4.915 ± 0.078	3.221GluSer: 3.221 ± 0.046	2.964GluThr: 2.964 ± 0.042	3.415GluVal: 3.415 ± 0.055	0.845GluTrp: 0.845 ± 0.021	1.412GluTyr: 1.412 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	3.833PheAla: 3.833 ± 0.056	0.526PheCys: 0.526 ± 0.02	2.627PheAsp: 2.627 ± 0.042	2.28PheGlu: 2.28 ± 0.043	1.321PhePhe: 1.321 ± 0.032	3.28PheGly: 3.28 ± 0.056	0.656PheHis: 0.656 ± 0.022	1.452PheIle: 1.452 ± 0.029	0.89PheLys: 0.89 ± 0.028	2.872PheLeu: 2.872 ± 0.054	0.575PheMet: 0.575 ± 0.02	1.168PheAsn: 1.168 ± 0.033	1.533PhePro: 1.533 ± 0.037	1.032PheGln: 1.032 ± 0.025	2.403PheArg: 2.403 ± 0.04	2.23PheSer: 2.23 ± 0.043	1.905PheThr: 1.905 ± 0.035	3.032PheVal: 3.032 ± 0.048	0.552PheTrp: 0.552 ± 0.019	0.86PheTyr: 0.86 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	9.406GlyAla: 9.406 ± 0.104	1.35GlyCys: 1.35 ± 0.037	5.548GlyAsp: 5.548 ± 0.096	4.623GlyGlu: 4.623 ± 0.057	2.953GlyPhe: 2.953 ± 0.044	8.826GlyGly: 8.826 ± 0.152	1.619GlyHis: 1.619 ± 0.04	3.397GlyIle: 3.397 ± 0.06	2.752GlyLys: 2.752 ± 0.056	7.573GlyLeu: 7.573 ± 0.085	1.875GlyMet: 1.875 ± 0.038	2.532GlyAsn: 2.532 ± 0.07	3.925GlyPro: 3.925 ± 0.052	2.994GlyGln: 2.994 ± 0.046	6.669GlyArg: 6.669 ± 0.08	4.912GlySer: 4.912 ± 0.063	4.571GlyThr: 4.571 ± 0.076	6.777GlyVal: 6.777 ± 0.068	1.364GlyTrp: 1.364 ± 0.031	1.998GlyTyr: 1.998 ± 0.044	0.0GlyXaa: 0.0 ± 0.0
His	2.456HisAla: 2.456 ± 0.045	0.302HisCys: 0.302 ± 0.016	1.205HisAsp: 1.205 ± 0.028	1.203HisGlu: 1.203 ± 0.029	0.783HisPhe: 0.783 ± 0.024	1.698HisGly: 1.698 ± 0.036	0.468HisHis: 0.468 ± 0.017	0.799HisIle: 0.799 ± 0.023	0.482HisLys: 0.482 ± 0.02	1.842HisLeu: 1.842 ± 0.034	0.348HisMet: 0.348 ± 0.015	0.46HisAsn: 0.46 ± 0.019	1.268HisPro: 1.268 ± 0.03	0.551HisGln: 0.551 ± 0.018	1.516HisArg: 1.516 ± 0.037	0.95HisSer: 0.95 ± 0.023	0.927HisThr: 0.927 ± 0.024	1.662HisVal: 1.662 ± 0.036	0.324HisTrp: 0.324 ± 0.017	0.541HisTyr: 0.541 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	5.769IleAla: 5.769 ± 0.067	0.62IleCys: 0.62 ± 0.021	3.47IleAsp: 3.47 ± 0.048	3.375IleGlu: 3.375 ± 0.05	1.291IlePhe: 1.291 ± 0.03	4.079IleGly: 4.079 ± 0.059	0.922IleHis: 0.922 ± 0.022	1.769IleIle: 1.769 ± 0.039	1.144IleLys: 1.144 ± 0.032	3.791IleLeu: 3.791 ± 0.056	0.643IleMet: 0.643 ± 0.022	1.625IleAsn: 1.625 ± 0.045	2.172IlePro: 2.172 ± 0.038	1.241IleGln: 1.241 ± 0.031	3.388IleArg: 3.388 ± 0.058	2.391IleSer: 2.391 ± 0.042	2.299IleThr: 2.299 ± 0.043	3.717IleVal: 3.717 ± 0.055	0.547IleTrp: 0.547 ± 0.017	1.036IleTyr: 1.036 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	3.006LysAla: 3.006 ± 0.064	0.307LysCys: 0.307 ± 0.015	1.346LysAsp: 1.346 ± 0.038	1.382LysGlu: 1.382 ± 0.04	0.846LysPhe: 0.846 ± 0.025	1.623LysGly: 1.623 ± 0.04	0.597LysHis: 0.597 ± 0.022	1.353LysIle: 1.353 ± 0.037	1.273LysLys: 1.273 ± 0.041	3.346LysLeu: 3.346 ± 0.059	0.689LysMet: 0.689 ± 0.019	0.775LysAsn: 0.775 ± 0.025	1.884LysPro: 1.884 ± 0.048	1.096LysGln: 1.096 ± 0.036	2.268LysArg: 2.268 ± 0.043	1.661LysSer: 1.661 ± 0.038	1.609LysThr: 1.609 ± 0.041	1.766LysVal: 1.766 ± 0.038	0.367LysTrp: 0.367 ± 0.017	0.66LysTyr: 0.66 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	13.104LeuAla: 13.104 ± 0.135	1.265LeuCys: 1.265 ± 0.031	5.944LeuAsp: 5.944 ± 0.064	5.057LeuGlu: 5.057 ± 0.076	3.066LeuPhe: 3.066 ± 0.049	7.583LeuGly: 7.583 ± 0.076	2.004LeuHis: 2.004 ± 0.038	4.401LeuIle: 4.401 ± 0.051	2.939LeuLys: 2.939 ± 0.049	10.408LeuLeu: 10.408 ± 0.126	1.801LeuMet: 1.801 ± 0.036	2.713LeuAsn: 2.713 ± 0.041	5.359LeuPro: 5.359 ± 0.065	2.708LeuGln: 2.708 ± 0.048	8.336LeuArg: 8.336 ± 0.09	5.932LeuSer: 5.932 ± 0.063	5.633LeuThr: 5.633 ± 0.067	6.735LeuVal: 6.735 ± 0.08	1.26LeuTrp: 1.26 ± 0.03	2.089LeuTyr: 2.089 ± 0.04	0.0LeuXaa: 0.0 ± 0.0
Met	2.063MetAla: 2.063 ± 0.039	0.222MetCys: 0.222 ± 0.012	0.865MetAsp: 0.865 ± 0.027	0.796MetGlu: 0.796 ± 0.024	0.639MetPhe: 0.639 ± 0.022	1.329MetGly: 1.329 ± 0.034	0.479MetHis: 0.479 ± 0.018	1.02MetIle: 1.02 ± 0.028	0.788MetLys: 0.788 ± 0.024	2.304MetLeu: 2.304 ± 0.045	0.438MetMet: 0.438 ± 0.02	0.798MetAsn: 0.798 ± 0.024	1.173MetPro: 1.173 ± 0.027	0.781MetGln: 0.781 ± 0.025	1.603MetArg: 1.603 ± 0.033	1.383MetSer: 1.383 ± 0.03	1.311MetThr: 1.311 ± 0.029	1.196MetVal: 1.196 ± 0.03	0.203MetTrp: 0.203 ± 0.013	0.36MetTyr: 0.36 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.142AsnAla: 3.142 ± 0.05	0.589AsnCys: 0.589 ± 0.03	1.739AsnAsp: 1.739 ± 0.048	1.35AsnGlu: 1.35 ± 0.033	0.974AsnPhe: 0.974 ± 0.029	2.859AsnGly: 2.859 ± 0.07	0.492AsnHis: 0.492 ± 0.017	1.334AsnIle: 1.334 ± 0.032	0.604AsnLys: 0.604 ± 0.022	2.505AsnLeu: 2.505 ± 0.046	0.476AsnMet: 0.476 ± 0.019	0.973AsnAsn: 0.973 ± 0.036	2.227AsnPro: 2.227 ± 0.052	0.91AsnGln: 0.91 ± 0.033	1.881AsnArg: 1.881 ± 0.036	1.465AsnSer: 1.465 ± 0.04	1.445AsnThr: 1.445 ± 0.041	2.243AsnVal: 2.243 ± 0.044	0.468AsnTrp: 0.468 ± 0.019	0.706AsnTyr: 0.706 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	7.735ProAla: 7.735 ± 0.091	0.611ProCys: 0.611 ± 0.028	3.768ProAsp: 3.768 ± 0.058	3.291ProGlu: 3.291 ± 0.049	1.85ProPhe: 1.85 ± 0.036	5.013ProGly: 5.013 ± 0.054	1.124ProHis: 1.124 ± 0.027	2.407ProIle: 2.407 ± 0.039	1.352ProLys: 1.352 ± 0.032	5.135ProLeu: 5.135 ± 0.066	1.135ProMet: 1.135 ± 0.026	1.514ProAsn: 1.514 ± 0.038	4.416ProPro: 4.416 ± 0.078	1.903ProGln: 1.903 ± 0.036	3.88ProArg: 3.88 ± 0.059	2.969ProSer: 2.969 ± 0.044	3.078ProThr: 3.078 ± 0.049	3.772ProVal: 3.772 ± 0.051	0.697ProTrp: 0.697 ± 0.023	1.057ProTyr: 1.057 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	3.714GlnAla: 3.714 ± 0.061	0.377GlnCys: 0.377 ± 0.017	1.307GlnAsp: 1.307 ± 0.029	1.411GlnGlu: 1.411 ± 0.037	1.238GlnPhe: 1.238 ± 0.026	1.867GlnGly: 1.867 ± 0.032	0.745GlnHis: 0.745 ± 0.024	1.746GlnIle: 1.746 ± 0.036	1.034GlnLys: 1.034 ± 0.03	3.229GlnLeu: 3.229 ± 0.052	0.726GlnMet: 0.726 ± 0.02	0.929GlnAsn: 0.929 ± 0.029	2.209GlnPro: 2.209 ± 0.043	1.451GlnGln: 1.451 ± 0.043	3.321GlnArg: 3.321 ± 0.056	1.833GlnSer: 1.833 ± 0.034	1.933GlnThr: 1.933 ± 0.04	2.384GlnVal: 2.384 ± 0.043	0.501GlnTrp: 0.501 ± 0.02	0.743GlnTyr: 0.743 ± 0.025	0.0GlnXaa: 0.0 ± 0.0
Arg	7.919ArgAla: 7.919 ± 0.097	1.069ArgCys: 1.069 ± 0.029	4.496ArgAsp: 4.496 ± 0.058	4.83ArgGlu: 4.83 ± 0.079	3.2ArgPhe: 3.2 ± 0.05	5.738ArgGly: 5.738 ± 0.07	1.608ArgHis: 1.608 ± 0.035	3.849ArgIle: 3.849 ± 0.051	2.233ArgLys: 2.233 ± 0.046	8.239ArgLeu: 8.239 ± 0.098	1.842ArgMet: 1.842 ± 0.033	1.97ArgAsn: 1.97 ± 0.039	4.257ArgPro: 4.257 ± 0.056	2.671ArgGln: 2.671 ± 0.05	7.744ArgArg: 7.744 ± 0.1	4.161ArgSer: 4.161 ± 0.054	3.714ArgThr: 3.714 ± 0.058	6.033ArgVal: 6.033 ± 0.068	1.35ArgTrp: 1.35 ± 0.034	2.028ArgTyr: 2.028 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	6.659SerAla: 6.659 ± 0.079	0.644SerCys: 0.644 ± 0.025	3.293SerAsp: 3.293 ± 0.048	2.792SerGlu: 2.792 ± 0.052	1.998SerPhe: 1.998 ± 0.038	5.918SerGly: 5.918 ± 0.079	0.991SerHis: 0.991 ± 0.027	2.404SerIle: 2.404 ± 0.044	1.358SerLys: 1.358 ± 0.033	5.202SerLeu: 5.202 ± 0.066	1.033SerMet: 1.033 ± 0.026	1.59SerAsn: 1.59 ± 0.039	3.399SerPro: 3.399 ± 0.046	1.779SerGln: 1.779 ± 0.033	4.019SerArg: 4.019 ± 0.052	3.22SerSer: 3.22 ± 0.062	2.946SerThr: 2.946 ± 0.045	3.996SerVal: 3.996 ± 0.058	0.77SerTrp: 0.77 ± 0.025	1.192SerTyr: 1.192 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	6.002ThrAla: 6.002 ± 0.065	0.733ThrCys: 0.733 ± 0.028	2.876ThrAsp: 2.876 ± 0.051	2.178ThrGlu: 2.178 ± 0.041	1.954ThrPhe: 1.954 ± 0.041	4.834ThrGly: 4.834 ± 0.065	1.051ThrHis: 1.051 ± 0.029	2.726ThrIle: 2.726 ± 0.049	1.119ThrLys: 1.119 ± 0.03	5.748ThrLeu: 5.748 ± 0.068	0.943ThrMet: 0.943 ± 0.022	1.543ThrAsn: 1.543 ± 0.042	3.791ThrPro: 3.791 ± 0.054	1.688ThrGln: 1.688 ± 0.037	3.801ThrArg: 3.801 ± 0.049	2.808ThrSer: 2.808 ± 0.042	3.026ThrThr: 3.026 ± 0.061	3.901ThrVal: 3.901 ± 0.069	0.917ThrTrp: 0.917 ± 0.03	1.376ThrTyr: 1.376 ± 0.043	0.0ThrXaa: 0.0 ± 0.0
Val	8.633ValAla: 8.633 ± 0.079	1.093ValCys: 1.093 ± 0.029	4.212ValAsp: 4.212 ± 0.052	4.413ValGlu: 4.413 ± 0.065	2.716ValPhe: 2.716 ± 0.045	5.774ValGly: 5.774 ± 0.059	1.406ValHis: 1.406 ± 0.031	3.503ValIle: 3.503 ± 0.054	1.894ValLys: 1.894 ± 0.039	7.49ValLeu: 7.49 ± 0.084	1.419ValMet: 1.419 ± 0.029	2.234ValAsn: 2.234 ± 0.043	3.546ValPro: 3.546 ± 0.05	2.303ValGln: 2.303 ± 0.04	5.897ValArg: 5.897 ± 0.078	4.189ValSer: 4.189 ± 0.055	4.108ValThr: 4.108 ± 0.061	5.93ValVal: 5.93 ± 0.067	1.092ValTrp: 1.092 ± 0.029	1.81ValTyr: 1.81 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	1.329TrpAla: 1.329 ± 0.036	0.194TrpCys: 0.194 ± 0.012	0.8TrpAsp: 0.8 ± 0.033	0.675TrpGlu: 0.675 ± 0.022	0.552TrpPhe: 0.552 ± 0.018	0.928TrpGly: 0.928 ± 0.025	0.338TrpHis: 0.338 ± 0.016	0.776TrpIle: 0.776 ± 0.021	0.518TrpLys: 0.518 ± 0.02	1.661TrpLeu: 1.661 ± 0.032	0.346TrpMet: 0.346 ± 0.012	0.57TrpAsn: 0.57 ± 0.022	0.793TrpPro: 0.793 ± 0.024	0.607TrpGln: 0.607 ± 0.021	1.35TrpArg: 1.35 ± 0.034	0.961TrpSer: 0.961 ± 0.027	0.982TrpThr: 0.982 ± 0.034	0.885TrpVal: 0.885 ± 0.026	0.27TrpTrp: 0.27 ± 0.014	0.366TrpTyr: 0.366 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.459TyrAla: 2.459 ± 0.046	0.361TyrCys: 0.361 ± 0.015	1.717TyrAsp: 1.717 ± 0.048	1.399TyrGlu: 1.399 ± 0.034	1.0TyrPhe: 1.0 ± 0.026	1.835TyrGly: 1.835 ± 0.042	0.507TyrHis: 0.507 ± 0.019	0.853TyrIle: 0.853 ± 0.024	0.594TyrLys: 0.594 ± 0.018	2.152TyrLeu: 2.152 ± 0.043	0.373TyrMet: 0.373 ± 0.015	0.691TyrAsn: 0.691 ± 0.026	1.092TyrPro: 1.092 ± 0.028	0.8TyrGln: 0.8 ± 0.028	1.942TyrArg: 1.942 ± 0.037	1.324TyrSer: 1.324 ± 0.034	1.148TyrThr: 1.148 ± 0.032	1.899TyrVal: 1.899 ± 0.039	0.416TyrTrp: 0.416 ± 0.016	0.762TyrTyr: 0.762 ± 0.024	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4315 proteins (1565664 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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