Amino acid dipepetide frequency for Gordonia phage Powerball

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.12AlaAla: 18.12 ± 1.735
1.032AlaCys: 1.032 ± 0.287
9.092AlaAsp: 9.092 ± 0.702
9.221AlaGlu: 9.221 ± 0.849
2.837AlaPhe: 2.837 ± 0.466
9.479AlaGly: 9.479 ± 0.799
2.128AlaHis: 2.128 ± 0.37
4.578AlaIle: 4.578 ± 0.477
4.385AlaLys: 4.385 ± 0.616
10.769AlaLeu: 10.769 ± 0.811
3.289AlaMet: 3.289 ± 0.615
4.127AlaAsn: 4.127 ± 0.608
5.417AlaPro: 5.417 ± 0.786
5.546AlaGln: 5.546 ± 0.828
9.286AlaArg: 9.286 ± 0.883
5.803AlaSer: 5.803 ± 0.773
8.383AlaThr: 8.383 ± 0.963
8.254AlaVal: 8.254 ± 1.087
1.677AlaTrp: 1.677 ± 0.346
2.386AlaTyr: 2.386 ± 0.353
0.0AlaXaa: 0.0 ± 0.0
Cys
0.709CysAla: 0.709 ± 0.233
0.064CysCys: 0.064 ± 0.061
0.516CysAsp: 0.516 ± 0.167
0.387CysGlu: 0.387 ± 0.151
0.129CysPhe: 0.129 ± 0.096
0.903CysGly: 0.903 ± 0.262
0.193CysHis: 0.193 ± 0.117
0.258CysIle: 0.258 ± 0.172
0.129CysLys: 0.129 ± 0.091
0.58CysLeu: 0.58 ± 0.175
0.0CysMet: 0.0 ± 0.0
0.387CysAsn: 0.387 ± 0.154
0.516CysPro: 0.516 ± 0.205
0.129CysGln: 0.129 ± 0.09
0.838CysArg: 0.838 ± 0.237
0.516CysSer: 0.516 ± 0.196
0.709CysThr: 0.709 ± 0.291
0.451CysVal: 0.451 ± 0.188
0.193CysTrp: 0.193 ± 0.119
0.193CysTyr: 0.193 ± 0.112
0.0CysXaa: 0.0 ± 0.0
Asp
7.867AspAla: 7.867 ± 1.005
0.451AspCys: 0.451 ± 0.178
6.9AspAsp: 6.9 ± 1.028
4.32AspGlu: 4.32 ± 0.613
1.741AspPhe: 1.741 ± 0.362
5.159AspGly: 5.159 ± 0.664
1.612AspHis: 1.612 ± 0.345
2.063AspIle: 2.063 ± 0.346
1.806AspLys: 1.806 ± 0.327
7.802AspLeu: 7.802 ± 0.663
1.483AspMet: 1.483 ± 0.37
1.999AspAsn: 1.999 ± 0.354
4.32AspPro: 4.32 ± 0.633
2.257AspGln: 2.257 ± 0.423
4.127AspArg: 4.127 ± 0.551
3.289AspSer: 3.289 ± 0.606
3.869AspThr: 3.869 ± 0.596
4.901AspVal: 4.901 ± 0.62
1.87AspTrp: 1.87 ± 0.338
1.87AspTyr: 1.87 ± 0.303
0.0AspXaa: 0.0 ± 0.0
Glu
7.158GluAla: 7.158 ± 0.819
0.838GluCys: 0.838 ± 0.256
2.837GluAsp: 2.837 ± 0.418
3.031GluGlu: 3.031 ± 0.49
1.999GluPhe: 1.999 ± 0.432
2.902GluGly: 2.902 ± 0.465
0.903GluHis: 0.903 ± 0.266
2.966GluIle: 2.966 ± 0.528
1.806GluLys: 1.806 ± 0.419
5.546GluLeu: 5.546 ± 0.769
1.161GluMet: 1.161 ± 0.39
1.225GluAsn: 1.225 ± 0.348
2.644GluPro: 2.644 ± 0.478
3.095GluGln: 3.095 ± 0.482
4.514GluArg: 4.514 ± 0.723
2.773GluSer: 2.773 ± 0.456
3.74GluThr: 3.74 ± 0.448
3.933GluVal: 3.933 ± 0.538
1.161GluTrp: 1.161 ± 0.285
1.29GluTyr: 1.29 ± 0.322
0.0GluXaa: 0.0 ± 0.0
Phe
2.773PheAla: 2.773 ± 0.457
0.129PheCys: 0.129 ± 0.089
2.321PheAsp: 2.321 ± 0.337
1.161PheGlu: 1.161 ± 0.223
0.903PhePhe: 0.903 ± 0.325
2.45PheGly: 2.45 ± 0.36
0.516PheHis: 0.516 ± 0.233
0.645PheIle: 0.645 ± 0.215
0.645PheLys: 0.645 ± 0.179
1.483PheLeu: 1.483 ± 0.355
0.387PheMet: 0.387 ± 0.139
0.774PheAsn: 0.774 ± 0.22
1.419PhePro: 1.419 ± 0.3
0.58PheGln: 0.58 ± 0.265
1.999PheArg: 1.999 ± 0.5
1.225PheSer: 1.225 ± 0.239
1.806PheThr: 1.806 ± 0.344
1.483PheVal: 1.483 ± 0.386
0.645PheTrp: 0.645 ± 0.181
0.58PheTyr: 0.58 ± 0.191
0.0PheXaa: 0.0 ± 0.0
Gly
8.834GlyAla: 8.834 ± 0.851
0.58GlyCys: 0.58 ± 0.219
5.288GlyAsp: 5.288 ± 0.511
4.772GlyGlu: 4.772 ± 0.477
2.063GlyPhe: 2.063 ± 0.394
7.673GlyGly: 7.673 ± 1.041
1.29GlyHis: 1.29 ± 0.258
3.998GlyIle: 3.998 ± 0.514
3.16GlyLys: 3.16 ± 0.5
6.255GlyLeu: 6.255 ± 0.706
2.579GlyMet: 2.579 ± 0.52
2.966GlyAsn: 2.966 ± 0.419
4.191GlyPro: 4.191 ± 0.595
2.966GlyGln: 2.966 ± 0.533
6.771GlyArg: 6.771 ± 0.731
4.707GlySer: 4.707 ± 0.927
6.577GlyThr: 6.577 ± 0.692
6.126GlyVal: 6.126 ± 0.629
1.29GlyTrp: 1.29 ± 0.256
1.934GlyTyr: 1.934 ± 0.417
0.0GlyXaa: 0.0 ± 0.0
His
2.321HisAla: 2.321 ± 0.401
0.0HisCys: 0.0 ± 0.0
1.419HisAsp: 1.419 ± 0.359
1.161HisGlu: 1.161 ± 0.32
0.193HisPhe: 0.193 ± 0.115
1.806HisGly: 1.806 ± 0.329
0.387HisHis: 0.387 ± 0.18
1.161HisIle: 1.161 ± 0.386
0.387HisLys: 0.387 ± 0.158
1.806HisLeu: 1.806 ± 0.453
0.193HisMet: 0.193 ± 0.104
0.387HisAsn: 0.387 ± 0.17
1.483HisPro: 1.483 ± 0.361
1.096HisGln: 1.096 ± 0.245
1.354HisArg: 1.354 ± 0.342
1.096HisSer: 1.096 ± 0.369
1.87HisThr: 1.87 ± 0.362
1.741HisVal: 1.741 ± 0.361
0.451HisTrp: 0.451 ± 0.16
0.903HisTyr: 0.903 ± 0.257
0.0HisXaa: 0.0 ± 0.0
Ile
7.029IleAla: 7.029 ± 0.792
0.258IleCys: 0.258 ± 0.136
4.191IleAsp: 4.191 ± 0.519
2.837IleGlu: 2.837 ± 0.501
0.903IlePhe: 0.903 ± 0.25
3.611IleGly: 3.611 ± 0.569
1.032IleHis: 1.032 ± 0.254
1.032IleIle: 1.032 ± 0.284
0.774IleLys: 0.774 ± 0.234
2.321IleLeu: 2.321 ± 0.38
1.032IleMet: 1.032 ± 0.277
1.354IleAsn: 1.354 ± 0.331
2.579IlePro: 2.579 ± 0.474
1.612IleGln: 1.612 ± 0.32
3.16IleArg: 3.16 ± 0.425
2.515IleSer: 2.515 ± 0.357
2.708IleThr: 2.708 ± 0.577
3.353IleVal: 3.353 ± 0.497
0.322IleTrp: 0.322 ± 0.137
1.225IleTyr: 1.225 ± 0.298
0.0IleXaa: 0.0 ± 0.0
Lys
4.514LysAla: 4.514 ± 0.645
0.129LysCys: 0.129 ± 0.092
1.161LysAsp: 1.161 ± 0.267
1.548LysGlu: 1.548 ± 0.364
0.838LysPhe: 0.838 ± 0.234
2.321LysGly: 2.321 ± 0.48
0.58LysHis: 0.58 ± 0.199
1.29LysIle: 1.29 ± 0.276
1.419LysLys: 1.419 ± 0.474
2.386LysLeu: 2.386 ± 0.503
0.516LysMet: 0.516 ± 0.17
0.709LysAsn: 0.709 ± 0.249
2.321LysPro: 2.321 ± 0.362
1.29LysGln: 1.29 ± 0.381
2.128LysArg: 2.128 ± 0.446
2.257LysSer: 2.257 ± 0.514
2.773LysThr: 2.773 ± 0.505
2.45LysVal: 2.45 ± 0.372
0.645LysTrp: 0.645 ± 0.234
0.516LysTyr: 0.516 ± 0.193
0.0LysXaa: 0.0 ± 0.0
Leu
11.027LeuAla: 11.027 ± 1.003
0.58LeuCys: 0.58 ± 0.237
5.288LeuAsp: 5.288 ± 0.65
4.062LeuGlu: 4.062 ± 0.578
1.87LeuPhe: 1.87 ± 0.471
6.513LeuGly: 6.513 ± 0.604
1.806LeuHis: 1.806 ± 0.408
4.256LeuIle: 4.256 ± 0.519
1.934LeuLys: 1.934 ± 0.452
5.803LeuLeu: 5.803 ± 0.585
2.063LeuMet: 2.063 ± 0.373
1.806LeuAsn: 1.806 ± 0.31
4.256LeuPro: 4.256 ± 0.491
2.966LeuGln: 2.966 ± 0.532
6.706LeuArg: 6.706 ± 0.654
4.191LeuSer: 4.191 ± 0.465
6.19LeuThr: 6.19 ± 0.746
5.803LeuVal: 5.803 ± 0.766
1.806LeuTrp: 1.806 ± 0.361
1.483LeuTyr: 1.483 ± 0.309
0.0LeuXaa: 0.0 ± 0.0
Met
2.837MetAla: 2.837 ± 0.636
0.258MetCys: 0.258 ± 0.116
0.967MetAsp: 0.967 ± 0.226
0.193MetGlu: 0.193 ± 0.113
0.645MetPhe: 0.645 ± 0.213
1.483MetGly: 1.483 ± 0.38
0.774MetHis: 0.774 ± 0.217
0.451MetIle: 0.451 ± 0.169
0.903MetLys: 0.903 ± 0.245
1.612MetLeu: 1.612 ± 0.346
0.387MetMet: 0.387 ± 0.128
0.709MetAsn: 0.709 ± 0.199
1.419MetPro: 1.419 ± 0.312
0.58MetGln: 0.58 ± 0.224
1.87MetArg: 1.87 ± 0.344
1.806MetSer: 1.806 ± 0.395
2.515MetThr: 2.515 ± 0.373
1.225MetVal: 1.225 ± 0.272
0.322MetTrp: 0.322 ± 0.228
0.322MetTyr: 0.322 ± 0.151
0.0MetXaa: 0.0 ± 0.0
Asn
2.966AsnAla: 2.966 ± 0.634
0.064AsnCys: 0.064 ± 0.07
1.806AsnAsp: 1.806 ± 0.307
1.354AsnGlu: 1.354 ± 0.274
0.709AsnPhe: 0.709 ± 0.178
2.708AsnGly: 2.708 ± 0.489
1.29AsnHis: 1.29 ± 0.287
1.29AsnIle: 1.29 ± 0.259
0.709AsnLys: 0.709 ± 0.263
1.87AsnLeu: 1.87 ± 0.424
0.387AsnMet: 0.387 ± 0.131
0.709AsnAsn: 0.709 ± 0.23
2.128AsnPro: 2.128 ± 0.299
0.838AsnGln: 0.838 ± 0.236
2.515AsnArg: 2.515 ± 0.401
1.741AsnSer: 1.741 ± 0.308
1.87AsnThr: 1.87 ± 0.364
1.29AsnVal: 1.29 ± 0.31
0.709AsnTrp: 0.709 ± 0.224
0.387AsnTyr: 0.387 ± 0.168
0.0AsnXaa: 0.0 ± 0.0
Pro
6.706ProAla: 6.706 ± 0.638
0.645ProCys: 0.645 ± 0.252
4.514ProAsp: 4.514 ± 0.684
3.676ProGlu: 3.676 ± 0.483
1.419ProPhe: 1.419 ± 0.391
5.481ProGly: 5.481 ± 0.792
0.645ProHis: 0.645 ± 0.213
2.386ProIle: 2.386 ± 0.37
1.677ProLys: 1.677 ± 0.312
3.676ProLeu: 3.676 ± 0.498
1.354ProMet: 1.354 ± 0.261
1.741ProAsn: 1.741 ± 0.35
2.321ProPro: 2.321 ± 0.404
1.161ProGln: 1.161 ± 0.287
3.16ProArg: 3.16 ± 0.521
2.902ProSer: 2.902 ± 0.361
4.901ProThr: 4.901 ± 0.636
4.643ProVal: 4.643 ± 0.562
1.032ProTrp: 1.032 ± 0.219
0.58ProTyr: 0.58 ± 0.166
0.0ProXaa: 0.0 ± 0.0
Gln
5.094GlnAla: 5.094 ± 0.761
0.193GlnCys: 0.193 ± 0.098
1.612GlnAsp: 1.612 ± 0.34
1.354GlnGlu: 1.354 ± 0.264
1.225GlnPhe: 1.225 ± 0.305
2.257GlnGly: 2.257 ± 0.584
0.774GlnHis: 0.774 ± 0.232
1.999GlnIle: 1.999 ± 0.505
1.161GlnLys: 1.161 ± 0.339
3.933GlnLeu: 3.933 ± 0.462
0.903GlnMet: 0.903 ± 0.237
0.709GlnAsn: 0.709 ± 0.29
1.354GlnPro: 1.354 ± 0.263
1.032GlnGln: 1.032 ± 0.383
3.804GlnArg: 3.804 ± 0.628
1.999GlnSer: 1.999 ± 0.317
2.644GlnThr: 2.644 ± 0.443
3.16GlnVal: 3.16 ± 0.522
0.709GlnTrp: 0.709 ± 0.201
0.58GlnTyr: 0.58 ± 0.239
0.0GlnXaa: 0.0 ± 0.0
Arg
7.996ArgAla: 7.996 ± 0.904
0.58ArgCys: 0.58 ± 0.185
5.417ArgAsp: 5.417 ± 0.853
3.804ArgGlu: 3.804 ± 0.54
1.483ArgPhe: 1.483 ± 0.256
5.417ArgGly: 5.417 ± 0.673
1.677ArgHis: 1.677 ± 0.387
4.191ArgIle: 4.191 ± 0.678
2.644ArgLys: 2.644 ± 0.335
7.287ArgLeu: 7.287 ± 0.567
1.677ArgMet: 1.677 ± 0.367
2.386ArgAsn: 2.386 ± 0.361
4.191ArgPro: 4.191 ± 0.68
2.837ArgGln: 2.837 ± 0.438
5.868ArgArg: 5.868 ± 0.888
4.449ArgSer: 4.449 ± 0.647
5.417ArgThr: 5.417 ± 0.53
4.578ArgVal: 4.578 ± 0.567
1.548ArgTrp: 1.548 ± 0.329
1.806ArgTyr: 1.806 ± 0.364
0.0ArgXaa: 0.0 ± 0.0
Ser
7.544SerAla: 7.544 ± 1.01
0.193SerCys: 0.193 ± 0.119
3.418SerAsp: 3.418 ± 0.436
2.708SerGlu: 2.708 ± 0.387
1.548SerPhe: 1.548 ± 0.31
6.513SerGly: 6.513 ± 0.812
1.225SerHis: 1.225 ± 0.343
2.579SerIle: 2.579 ± 0.367
1.806SerLys: 1.806 ± 0.326
3.74SerLeu: 3.74 ± 0.492
1.032SerMet: 1.032 ± 0.27
1.161SerAsn: 1.161 ± 0.229
2.515SerPro: 2.515 ± 0.455
2.515SerGln: 2.515 ± 0.709
3.031SerArg: 3.031 ± 0.549
3.031SerSer: 3.031 ± 0.53
3.933SerThr: 3.933 ± 0.509
4.32SerVal: 4.32 ± 0.554
0.903SerTrp: 0.903 ± 0.245
1.225SerTyr: 1.225 ± 0.437
0.0SerXaa: 0.0 ± 0.0
Thr
9.35ThrAla: 9.35 ± 0.873
0.58ThrCys: 0.58 ± 0.211
4.449ThrAsp: 4.449 ± 0.607
3.676ThrGlu: 3.676 ± 0.509
1.29ThrPhe: 1.29 ± 0.274
7.673ThrGly: 7.673 ± 0.812
1.612ThrHis: 1.612 ± 0.306
4.256ThrIle: 4.256 ± 0.479
2.579ThrLys: 2.579 ± 0.451
5.481ThrLeu: 5.481 ± 0.551
1.096ThrMet: 1.096 ± 0.271
1.161ThrAsn: 1.161 ± 0.266
5.03ThrPro: 5.03 ± 0.607
2.063ThrGln: 2.063 ± 0.431
5.546ThrArg: 5.546 ± 0.772
3.224ThrSer: 3.224 ± 0.457
5.481ThrThr: 5.481 ± 0.743
6.642ThrVal: 6.642 ± 0.788
1.483ThrTrp: 1.483 ± 0.361
1.29ThrTyr: 1.29 ± 0.297
0.0ThrXaa: 0.0 ± 0.0
Val
8.125ValAla: 8.125 ± 0.939
0.709ValCys: 0.709 ± 0.236
6.384ValAsp: 6.384 ± 0.606
4.191ValGlu: 4.191 ± 0.449
1.419ValPhe: 1.419 ± 0.271
6.061ValGly: 6.061 ± 0.646
1.483ValHis: 1.483 ± 0.29
3.16ValIle: 3.16 ± 0.558
2.386ValLys: 2.386 ± 0.473
5.481ValLeu: 5.481 ± 0.704
1.096ValMet: 1.096 ± 0.228
2.45ValAsn: 2.45 ± 0.471
4.32ValPro: 4.32 ± 0.461
2.515ValGln: 2.515 ± 0.564
4.578ValArg: 4.578 ± 0.615
4.127ValSer: 4.127 ± 0.64
5.546ValThr: 5.546 ± 0.675
4.965ValVal: 4.965 ± 0.638
1.161ValTrp: 1.161 ± 0.41
1.741ValTyr: 1.741 ± 0.378
0.0ValXaa: 0.0 ± 0.0
Trp
1.741TrpAla: 1.741 ± 0.273
0.387TrpCys: 0.387 ± 0.18
0.903TrpAsp: 0.903 ± 0.249
0.645TrpGlu: 0.645 ± 0.204
0.516TrpPhe: 0.516 ± 0.219
1.548TrpGly: 1.548 ± 0.31
0.645TrpHis: 0.645 ± 0.258
0.709TrpIle: 0.709 ± 0.22
0.838TrpLys: 0.838 ± 0.219
1.354TrpLeu: 1.354 ± 0.419
0.387TrpMet: 0.387 ± 0.161
0.322TrpAsn: 0.322 ± 0.149
1.032TrpPro: 1.032 ± 0.207
0.645TrpGln: 0.645 ± 0.18
2.386TrpArg: 2.386 ± 0.451
1.29TrpSer: 1.29 ± 0.32
1.419TrpThr: 1.419 ± 0.307
1.096TrpVal: 1.096 ± 0.293
0.387TrpTrp: 0.387 ± 0.155
0.451TrpTyr: 0.451 ± 0.156
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.095TyrAla: 3.095 ± 0.412
0.129TyrCys: 0.129 ± 0.084
1.225TyrAsp: 1.225 ± 0.289
1.419TyrGlu: 1.419 ± 0.297
0.193TyrPhe: 0.193 ± 0.126
2.063TyrGly: 2.063 ± 0.383
0.645TyrHis: 0.645 ± 0.255
0.58TyrIle: 0.58 ± 0.179
0.645TyrLys: 0.645 ± 0.231
1.225TyrLeu: 1.225 ± 0.285
0.193TyrMet: 0.193 ± 0.107
0.258TyrAsn: 0.258 ± 0.13
1.161TyrPro: 1.161 ± 0.346
0.774TyrGln: 0.774 ± 0.227
1.677TyrArg: 1.677 ± 0.282
1.999TyrSer: 1.999 ± 0.338
1.612TyrThr: 1.612 ± 0.307
1.483TyrVal: 1.483 ± 0.279
0.387TyrTrp: 0.387 ± 0.187
0.709TyrTyr: 0.709 ± 0.241
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (15509 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski