Acanthamoeba castellanii str. Neff

Taxonomy: cellular organisms; Eukaryota; Amoebozoa; Discosea; Longamoebia; Centramoebida; Acanthamoebidae; Acanthamoeba; Acanthamoeba castellanii

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14939 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L8H3F0|L8H3F0_ACACA Homeobox domain containing protein OS=Acanthamoeba castellanii str. Neff OX=1257118 GN=ACA1_264140 PE=4 SV=1
MM1 pKa = 7.09TRR3 pKa = 11.84VALWALVGLILSIAVLSCSASDD25 pKa = 3.75GEE27 pKa = 4.6RR28 pKa = 11.84VKK30 pKa = 10.93RR31 pKa = 11.84PARR34 pKa = 11.84HH35 pKa = 6.66DD36 pKa = 4.01YY37 pKa = 11.05DD38 pKa = 4.28YY39 pKa = 11.66DD40 pKa = 5.7DD41 pKa = 5.77YY42 pKa = 11.92DD43 pKa = 4.22DD44 pKa = 5.95CEE46 pKa = 5.27RR47 pKa = 11.84DD48 pKa = 3.82DD49 pKa = 4.59NEE51 pKa = 4.97HH52 pKa = 6.54EE53 pKa = 4.51VACGYY58 pKa = 9.86GKK60 pKa = 10.04SWGSCEE66 pKa = 5.94DD67 pKa = 5.37DD68 pKa = 5.03DD69 pKa = 5.32DD70 pKa = 5.09CPPIALILEE79 pKa = 4.47LEE81 pKa = 4.16EE82 pKa = 4.23QEE84 pKa = 5.08GGEE87 pKa = 4.05DD88 pKa = 3.84SCWEE92 pKa = 4.16EE93 pKa = 4.81EE94 pKa = 4.73DD95 pKa = 5.11DD96 pKa = 4.18EE97 pKa = 4.8CTGGSPTTTTTSGGHH112 pKa = 4.35TTGGGHH118 pKa = 5.13STGGDD123 pKa = 3.07GSTGGEE129 pKa = 4.03NGGSDD134 pKa = 3.83TPVGAIVGGVLGGVFGAAAVGAAGAGAGYY163 pKa = 10.95ALYY166 pKa = 10.83RR167 pKa = 11.84FMQRR171 pKa = 11.84GGASPMPASAMDD183 pKa = 3.96GTEE186 pKa = 3.85MATMNDD192 pKa = 3.0NALFQSPTEE201 pKa = 3.91GHH203 pKa = 6.28TNDD206 pKa = 4.6LFQSGAMAAADD217 pKa = 3.43AVV219 pKa = 3.73

Molecular weight: 22.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L8GNE6|L8GNE6_ACACA Carrier superfamily protein OS=Acanthamoeba castellanii str. Neff OX=1257118 GN=ACA1_193100 PE=3 SV=1
MM1 pKa = 6.62TARR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84PPRR9 pKa = 11.84STSAPPPRR17 pKa = 11.84STTARR22 pKa = 11.84RR23 pKa = 11.84PPPRR27 pKa = 11.84STAARR32 pKa = 11.84RR33 pKa = 11.84PPPRR37 pKa = 11.84SPSAPLPRR45 pKa = 11.84SPSARR50 pKa = 11.84PRR52 pKa = 11.84CPGTMTRR59 pKa = 11.84RR60 pKa = 11.84SATGSATRR68 pKa = 11.84SAPRR72 pKa = 11.84SPTTPRR78 pKa = 11.84RR79 pKa = 11.84PPRR82 pKa = 11.84STALPPRR89 pKa = 11.84STPRR93 pKa = 11.84PPSIAPGSSASTPTEE108 pKa = 3.79HH109 pKa = 7.47

Molecular weight: 11.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14938

1

14939

6560542

16

4479

439.2

48.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.81 ± 0.025

1.408 ± 0.01

5.603 ± 0.016

6.968 ± 0.029

3.477 ± 0.013

6.888 ± 0.021

2.663 ± 0.013

3.783 ± 0.013

5.238 ± 0.023

9.353 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.088 ± 0.008

3.143 ± 0.011

5.528 ± 0.021

4.055 ± 0.016

6.339 ± 0.02

7.656 ± 0.027

5.622 ± 0.014

6.513 ± 0.018

1.352 ± 0.007

2.507 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski