Amino acid dipepetide frequency for Mycobacterium phage Lolly9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.953AlaAla: 11.953 ± 0.95
1.187AlaCys: 1.187 ± 0.259
5.669AlaAsp: 5.669 ± 0.561
7.735AlaGlu: 7.735 ± 0.687
3.384AlaPhe: 3.384 ± 0.346
8.438AlaGly: 8.438 ± 0.736
2.065AlaHis: 2.065 ± 0.381
4.746AlaIle: 4.746 ± 0.49
6.987AlaLys: 6.987 ± 0.578
10.283AlaLeu: 10.283 ± 0.694
3.032AlaMet: 3.032 ± 0.35
3.823AlaAsn: 3.823 ± 0.478
4.439AlaPro: 4.439 ± 0.523
4.702AlaGln: 4.702 ± 0.609
6.065AlaArg: 6.065 ± 0.434
5.142AlaSer: 5.142 ± 0.495
5.625AlaThr: 5.625 ± 0.53
6.328AlaVal: 6.328 ± 0.511
1.758AlaTrp: 1.758 ± 0.3
3.648AlaTyr: 3.648 ± 0.369
0.0AlaXaa: 0.0 ± 0.0
Cys
1.143CysAla: 1.143 ± 0.244
0.264CysCys: 0.264 ± 0.142
0.571CysAsp: 0.571 ± 0.17
0.483CysGlu: 0.483 ± 0.174
0.439CysPhe: 0.439 ± 0.156
1.23CysGly: 1.23 ± 0.306
0.176CysHis: 0.176 ± 0.089
0.22CysIle: 0.22 ± 0.09
0.352CysLys: 0.352 ± 0.135
0.879CysLeu: 0.879 ± 0.194
0.352CysMet: 0.352 ± 0.13
0.396CysAsn: 0.396 ± 0.17
0.571CysPro: 0.571 ± 0.154
0.308CysGln: 0.308 ± 0.121
0.879CysArg: 0.879 ± 0.229
0.835CysSer: 0.835 ± 0.213
0.703CysThr: 0.703 ± 0.166
1.055CysVal: 1.055 ± 0.297
0.176CysTrp: 0.176 ± 0.092
0.22CysTyr: 0.22 ± 0.087
0.0CysXaa: 0.0 ± 0.0
Asp
6.328AspAla: 6.328 ± 0.52
0.791AspCys: 0.791 ± 0.169
4.614AspAsp: 4.614 ± 0.561
3.999AspGlu: 3.999 ± 0.402
2.681AspPhe: 2.681 ± 0.382
5.845AspGly: 5.845 ± 0.427
1.274AspHis: 1.274 ± 0.257
2.769AspIle: 2.769 ± 0.376
2.988AspLys: 2.988 ± 0.362
5.449AspLeu: 5.449 ± 0.402
1.626AspMet: 1.626 ± 0.316
2.022AspAsn: 2.022 ± 0.341
3.823AspPro: 3.823 ± 0.412
2.461AspGln: 2.461 ± 0.337
3.12AspArg: 3.12 ± 0.361
3.516AspSer: 3.516 ± 0.362
3.428AspThr: 3.428 ± 0.455
4.395AspVal: 4.395 ± 0.499
1.494AspTrp: 1.494 ± 0.262
2.681AspTyr: 2.681 ± 0.469
0.0AspXaa: 0.0 ± 0.0
Glu
5.713GluAla: 5.713 ± 0.477
0.703GluCys: 0.703 ± 0.184
3.779GluAsp: 3.779 ± 0.382
2.813GluGlu: 2.813 ± 0.36
1.934GluPhe: 1.934 ± 0.406
3.735GluGly: 3.735 ± 0.408
1.494GluHis: 1.494 ± 0.316
2.373GluIle: 2.373 ± 0.323
1.934GluLys: 1.934 ± 0.334
7.691GluLeu: 7.691 ± 0.597
1.494GluMet: 1.494 ± 0.284
1.978GluAsn: 1.978 ± 0.276
2.505GluPro: 2.505 ± 0.412
2.593GluGln: 2.593 ± 0.319
4.351GluArg: 4.351 ± 0.632
2.681GluSer: 2.681 ± 0.355
2.549GluThr: 2.549 ± 0.318
3.691GluVal: 3.691 ± 0.474
1.406GluTrp: 1.406 ± 0.25
2.065GluTyr: 2.065 ± 0.341
0.0GluXaa: 0.0 ± 0.0
Phe
2.637PheAla: 2.637 ± 0.358
0.483PheCys: 0.483 ± 0.148
2.813PheAsp: 2.813 ± 0.379
1.45PheGlu: 1.45 ± 0.244
0.879PhePhe: 0.879 ± 0.174
3.12PheGly: 3.12 ± 0.33
0.835PheHis: 0.835 ± 0.191
1.362PheIle: 1.362 ± 0.281
1.714PheLys: 1.714 ± 0.264
2.593PheLeu: 2.593 ± 0.356
0.703PheMet: 0.703 ± 0.152
1.23PheAsn: 1.23 ± 0.223
1.978PhePro: 1.978 ± 0.285
1.23PheGln: 1.23 ± 0.305
1.45PheArg: 1.45 ± 0.268
1.714PheSer: 1.714 ± 0.249
2.241PheThr: 2.241 ± 0.304
2.681PheVal: 2.681 ± 0.317
0.747PheTrp: 0.747 ± 0.184
1.23PheTyr: 1.23 ± 0.201
0.0PheXaa: 0.0 ± 0.0
Gly
8.35GlyAla: 8.35 ± 0.736
0.923GlyCys: 0.923 ± 0.213
5.537GlyAsp: 5.537 ± 0.42
4.483GlyGlu: 4.483 ± 0.357
2.681GlyPhe: 2.681 ± 0.316
7.383GlyGly: 7.383 ± 0.814
1.582GlyHis: 1.582 ± 0.27
3.867GlyIle: 3.867 ± 0.393
4.131GlyLys: 4.131 ± 0.353
7.163GlyLeu: 7.163 ± 0.762
1.89GlyMet: 1.89 ± 0.252
3.648GlyAsn: 3.648 ± 0.432
3.955GlyPro: 3.955 ± 0.84
3.164GlyGln: 3.164 ± 0.343
4.087GlyArg: 4.087 ± 0.452
5.098GlySer: 5.098 ± 0.529
5.23GlyThr: 5.23 ± 0.682
5.977GlyVal: 5.977 ± 0.549
1.758GlyTrp: 1.758 ± 0.265
3.076GlyTyr: 3.076 ± 0.358
0.0GlyXaa: 0.0 ± 0.0
His
1.714HisAla: 1.714 ± 0.242
0.308HisCys: 0.308 ± 0.125
1.055HisAsp: 1.055 ± 0.215
1.406HisGlu: 1.406 ± 0.228
0.923HisPhe: 0.923 ± 0.208
1.802HisGly: 1.802 ± 0.291
0.571HisHis: 0.571 ± 0.164
0.747HisIle: 0.747 ± 0.197
0.571HisLys: 0.571 ± 0.155
1.582HisLeu: 1.582 ± 0.256
0.835HisMet: 0.835 ± 0.226
0.615HisAsn: 0.615 ± 0.15
0.835HisPro: 0.835 ± 0.165
0.308HisGln: 0.308 ± 0.113
1.274HisArg: 1.274 ± 0.247
0.835HisSer: 0.835 ± 0.179
0.879HisThr: 0.879 ± 0.186
1.538HisVal: 1.538 ± 0.305
0.483HisTrp: 0.483 ± 0.145
0.967HisTyr: 0.967 ± 0.226
0.0HisXaa: 0.0 ± 0.0
Ile
5.318IleAla: 5.318 ± 0.514
0.396IleCys: 0.396 ± 0.122
3.252IleAsp: 3.252 ± 0.334
2.769IleGlu: 2.769 ± 0.338
1.187IlePhe: 1.187 ± 0.23
3.472IleGly: 3.472 ± 0.448
0.527IleHis: 0.527 ± 0.18
1.626IleIle: 1.626 ± 0.269
1.978IleLys: 1.978 ± 0.271
2.637IleLeu: 2.637 ± 0.32
0.747IleMet: 0.747 ± 0.156
1.714IleAsn: 1.714 ± 0.319
2.593IlePro: 2.593 ± 0.359
1.406IleGln: 1.406 ± 0.245
2.681IleArg: 2.681 ± 0.32
1.802IleSer: 1.802 ± 0.267
2.857IleThr: 2.857 ± 0.424
3.12IleVal: 3.12 ± 0.429
0.571IleTrp: 0.571 ± 0.15
0.791IleTyr: 0.791 ± 0.183
0.0IleXaa: 0.0 ± 0.0
Lys
6.109LysAla: 6.109 ± 0.648
0.659LysCys: 0.659 ± 0.167
3.296LysAsp: 3.296 ± 0.391
2.153LysGlu: 2.153 ± 0.321
1.626LysPhe: 1.626 ± 0.316
4.351LysGly: 4.351 ± 0.468
1.011LysHis: 1.011 ± 0.2
1.802LysIle: 1.802 ± 0.317
2.373LysLys: 2.373 ± 0.363
4.087LysLeu: 4.087 ± 0.457
0.879LysMet: 0.879 ± 0.228
1.538LysAsn: 1.538 ± 0.231
2.417LysPro: 2.417 ± 0.317
1.846LysGln: 1.846 ± 0.302
3.911LysArg: 3.911 ± 0.416
2.637LysSer: 2.637 ± 0.307
2.241LysThr: 2.241 ± 0.295
3.472LysVal: 3.472 ± 0.388
1.143LysTrp: 1.143 ± 0.206
1.494LysTyr: 1.494 ± 0.261
0.0LysXaa: 0.0 ± 0.0
Leu
8.965LeuAla: 8.965 ± 0.576
0.967LeuCys: 0.967 ± 0.307
4.834LeuAsp: 4.834 ± 0.431
4.878LeuGlu: 4.878 ± 0.484
2.769LeuPhe: 2.769 ± 0.361
6.152LeuGly: 6.152 ± 0.641
1.538LeuHis: 1.538 ± 0.259
3.735LeuIle: 3.735 ± 0.381
4.878LeuLys: 4.878 ± 0.465
6.944LeuLeu: 6.944 ± 0.634
1.934LeuMet: 1.934 ± 0.295
3.955LeuAsn: 3.955 ± 0.532
4.746LeuPro: 4.746 ± 0.413
2.725LeuGln: 2.725 ± 0.344
5.098LeuArg: 5.098 ± 0.482
6.46LeuSer: 6.46 ± 0.669
5.977LeuThr: 5.977 ± 0.457
5.274LeuVal: 5.274 ± 0.487
1.89LeuTrp: 1.89 ± 0.32
1.802LeuTyr: 1.802 ± 0.384
0.0LeuXaa: 0.0 ± 0.0
Met
3.428MetAla: 3.428 ± 0.376
0.308MetCys: 0.308 ± 0.105
1.274MetAsp: 1.274 ± 0.272
0.791MetGlu: 0.791 ± 0.198
0.483MetPhe: 0.483 ± 0.171
1.978MetGly: 1.978 ± 0.259
0.923MetHis: 0.923 ± 0.184
0.967MetIle: 0.967 ± 0.21
0.703MetLys: 0.703 ± 0.173
2.153MetLeu: 2.153 ± 0.319
0.308MetMet: 0.308 ± 0.115
0.835MetAsn: 0.835 ± 0.169
1.406MetPro: 1.406 ± 0.236
1.011MetGln: 1.011 ± 0.221
1.758MetArg: 1.758 ± 0.267
2.065MetSer: 2.065 ± 0.338
1.846MetThr: 1.846 ± 0.252
1.494MetVal: 1.494 ± 0.274
0.308MetTrp: 0.308 ± 0.122
0.571MetTyr: 0.571 ± 0.159
0.0MetXaa: 0.0 ± 0.0
Asn
3.867AsnAla: 3.867 ± 0.409
0.22AsnCys: 0.22 ± 0.094
2.461AsnAsp: 2.461 ± 0.341
2.285AsnGlu: 2.285 ± 0.276
1.45AsnPhe: 1.45 ± 0.263
4.263AsnGly: 4.263 ± 0.405
0.703AsnHis: 0.703 ± 0.142
1.494AsnIle: 1.494 ± 0.334
1.143AsnLys: 1.143 ± 0.256
3.34AsnLeu: 3.34 ± 0.388
0.791AsnMet: 0.791 ± 0.158
1.23AsnAsn: 1.23 ± 0.216
2.549AsnPro: 2.549 ± 0.356
1.099AsnGln: 1.099 ± 0.191
2.417AsnArg: 2.417 ± 0.262
1.89AsnSer: 1.89 ± 0.321
2.153AsnThr: 2.153 ± 0.325
2.505AsnVal: 2.505 ± 0.34
0.703AsnTrp: 0.703 ± 0.145
0.967AsnTyr: 0.967 ± 0.251
0.0AsnXaa: 0.0 ± 0.0
Pro
4.658ProAla: 4.658 ± 0.547
0.571ProCys: 0.571 ± 0.139
3.867ProAsp: 3.867 ± 0.369
3.955ProGlu: 3.955 ± 0.425
1.934ProPhe: 1.934 ± 0.309
5.713ProGly: 5.713 ± 0.93
0.791ProHis: 0.791 ± 0.185
1.67ProIle: 1.67 ± 0.27
3.12ProLys: 3.12 ± 0.494
3.34ProLeu: 3.34 ± 0.385
1.318ProMet: 1.318 ± 0.302
1.846ProAsn: 1.846 ± 0.285
2.593ProPro: 2.593 ± 0.523
1.714ProGln: 1.714 ± 0.409
2.857ProArg: 2.857 ± 0.359
2.109ProSer: 2.109 ± 0.297
2.813ProThr: 2.813 ± 0.362
4.219ProVal: 4.219 ± 0.551
1.055ProTrp: 1.055 ± 0.226
0.923ProTyr: 0.923 ± 0.212
0.0ProXaa: 0.0 ± 0.0
Gln
5.23GlnAla: 5.23 ± 0.541
0.439GlnCys: 0.439 ± 0.135
2.109GlnAsp: 2.109 ± 0.35
1.582GlnGlu: 1.582 ± 0.301
1.274GlnPhe: 1.274 ± 0.231
2.549GlnGly: 2.549 ± 0.398
0.747GlnHis: 0.747 ± 0.17
1.846GlnIle: 1.846 ± 0.261
1.538GlnLys: 1.538 ± 0.299
2.637GlnLeu: 2.637 ± 0.384
1.143GlnMet: 1.143 ± 0.204
1.362GlnAsn: 1.362 ± 0.328
1.758GlnPro: 1.758 ± 0.273
0.879GlnGln: 0.879 ± 0.274
2.769GlnArg: 2.769 ± 0.305
1.802GlnSer: 1.802 ± 0.244
2.461GlnThr: 2.461 ± 0.382
2.9GlnVal: 2.9 ± 0.419
0.791GlnTrp: 0.791 ± 0.201
0.879GlnTyr: 0.879 ± 0.186
0.0GlnXaa: 0.0 ± 0.0
Arg
7.031ArgAla: 7.031 ± 0.525
0.659ArgCys: 0.659 ± 0.184
4.439ArgAsp: 4.439 ± 0.463
4.131ArgGlu: 4.131 ± 0.506
1.67ArgPhe: 1.67 ± 0.266
4.483ArgGly: 4.483 ± 0.419
0.879ArgHis: 0.879 ± 0.221
2.461ArgIle: 2.461 ± 0.382
3.648ArgLys: 3.648 ± 0.459
4.526ArgLeu: 4.526 ± 0.451
1.758ArgMet: 1.758 ± 0.29
2.417ArgAsn: 2.417 ± 0.326
2.109ArgPro: 2.109 ± 0.413
2.285ArgGln: 2.285 ± 0.324
3.911ArgArg: 3.911 ± 0.473
3.296ArgSer: 3.296 ± 0.37
2.417ArgThr: 2.417 ± 0.426
4.702ArgVal: 4.702 ± 0.499
1.626ArgTrp: 1.626 ± 0.303
1.714ArgTyr: 1.714 ± 0.308
0.0ArgXaa: 0.0 ± 0.0
Ser
6.768SerAla: 6.768 ± 0.608
0.396SerCys: 0.396 ± 0.134
3.296SerAsp: 3.296 ± 0.348
2.944SerGlu: 2.944 ± 0.359
1.582SerPhe: 1.582 ± 0.288
5.142SerGly: 5.142 ± 0.629
0.659SerHis: 0.659 ± 0.154
2.241SerIle: 2.241 ± 0.333
2.505SerLys: 2.505 ± 0.379
4.834SerLeu: 4.834 ± 0.548
1.274SerMet: 1.274 ± 0.207
1.934SerAsn: 1.934 ± 0.434
2.549SerPro: 2.549 ± 0.283
1.89SerGln: 1.89 ± 0.34
3.032SerArg: 3.032 ± 0.432
2.9SerSer: 2.9 ± 0.442
3.296SerThr: 3.296 ± 0.37
4.746SerVal: 4.746 ± 0.463
1.802SerTrp: 1.802 ± 0.382
1.67SerTyr: 1.67 ± 0.261
0.0SerXaa: 0.0 ± 0.0
Thr
6.196ThrAla: 6.196 ± 0.635
0.527ThrCys: 0.527 ± 0.166
4.131ThrAsp: 4.131 ± 0.422
2.417ThrGlu: 2.417 ± 0.305
2.549ThrPhe: 2.549 ± 0.401
4.702ThrGly: 4.702 ± 0.51
1.274ThrHis: 1.274 ± 0.264
3.032ThrIle: 3.032 ± 0.394
2.681ThrLys: 2.681 ± 0.307
5.01ThrLeu: 5.01 ± 0.443
1.318ThrMet: 1.318 ± 0.24
1.89ThrAsn: 1.89 ± 0.286
3.648ThrPro: 3.648 ± 0.475
1.714ThrGln: 1.714 ± 0.246
2.988ThrArg: 2.988 ± 0.404
2.593ThrSer: 2.593 ± 0.319
2.944ThrThr: 2.944 ± 0.42
5.098ThrVal: 5.098 ± 0.5
0.879ThrTrp: 0.879 ± 0.212
1.846ThrTyr: 1.846 ± 0.273
0.0ThrXaa: 0.0 ± 0.0
Val
6.372ValAla: 6.372 ± 0.57
1.055ValCys: 1.055 ± 0.347
4.746ValAsp: 4.746 ± 0.49
3.911ValGlu: 3.911 ± 0.445
2.065ValPhe: 2.065 ± 0.309
5.405ValGly: 5.405 ± 0.471
1.099ValHis: 1.099 ± 0.236
2.769ValIle: 2.769 ± 0.329
3.955ValLys: 3.955 ± 0.412
6.416ValLeu: 6.416 ± 0.603
1.846ValMet: 1.846 ± 0.29
2.725ValAsn: 2.725 ± 0.3
4.175ValPro: 4.175 ± 0.486
3.076ValGln: 3.076 ± 0.3
3.955ValArg: 3.955 ± 0.417
5.361ValSer: 5.361 ± 0.595
4.702ValThr: 4.702 ± 0.496
5.449ValVal: 5.449 ± 0.551
1.494ValTrp: 1.494 ± 0.224
1.846ValTyr: 1.846 ± 0.277
0.0ValXaa: 0.0 ± 0.0
Trp
2.285TrpAla: 2.285 ± 0.402
0.264TrpCys: 0.264 ± 0.124
1.626TrpAsp: 1.626 ± 0.301
1.187TrpGlu: 1.187 ± 0.24
0.747TrpPhe: 0.747 ± 0.199
1.23TrpGly: 1.23 ± 0.184
0.747TrpHis: 0.747 ± 0.174
0.747TrpIle: 0.747 ± 0.16
0.791TrpLys: 0.791 ± 0.172
1.626TrpLeu: 1.626 ± 0.207
0.571TrpMet: 0.571 ± 0.153
1.143TrpAsn: 1.143 ± 0.184
0.967TrpPro: 0.967 ± 0.207
1.143TrpGln: 1.143 ± 0.215
1.143TrpArg: 1.143 ± 0.24
1.099TrpSer: 1.099 ± 0.23
1.67TrpThr: 1.67 ± 0.272
1.582TrpVal: 1.582 ± 0.276
0.571TrpTrp: 0.571 ± 0.176
0.352TrpTyr: 0.352 ± 0.114
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.944TyrAla: 2.944 ± 0.344
0.132TyrCys: 0.132 ± 0.087
1.934TyrAsp: 1.934 ± 0.314
2.109TyrGlu: 2.109 ± 0.304
0.835TyrPhe: 0.835 ± 0.207
2.988TyrGly: 2.988 ± 0.381
0.352TyrHis: 0.352 ± 0.145
0.967TyrIle: 0.967 ± 0.197
1.055TyrLys: 1.055 ± 0.238
2.329TyrLeu: 2.329 ± 0.356
0.747TyrMet: 0.747 ± 0.242
1.23TyrAsn: 1.23 ± 0.193
1.45TyrPro: 1.45 ± 0.256
1.143TyrGln: 1.143 ± 0.225
2.461TyrArg: 2.461 ± 0.346
1.626TyrSer: 1.626 ± 0.285
1.406TyrThr: 1.406 ± 0.272
2.197TyrVal: 2.197 ± 0.254
0.835TyrTrp: 0.835 ± 0.178
1.011TyrTyr: 1.011 ± 0.183
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 130 proteins (22756 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski