Paracoccus phage vB_PyeM_Pyei1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3T0IK26|A0A3T0IK26_9CAUD Hint_2 domain-containing protein OS=Paracoccus phage vB_PyeM_Pyei1 OX=2500566 GN=pyei1_p11 PE=4 SV=1
MM1 pKa = 7.63SEE3 pKa = 4.29TEE5 pKa = 4.34NPDD8 pKa = 3.44TVPAGQPGAGEE19 pKa = 4.18NICRR23 pKa = 11.84KK24 pKa = 10.02CEE26 pKa = 3.75GSGQIDD32 pKa = 3.77GQPCPEE38 pKa = 4.49CDD40 pKa = 3.23GTGKK44 pKa = 10.28VVTGIGGAA52 pKa = 3.47

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q9R8E6|A0A3Q9R8E6_9CAUD Peptidoglycan-binding protein OS=Paracoccus phage vB_PyeM_Pyei1 OX=2500566 GN=pyei1_p72 PE=4 SV=1
MM1 pKa = 7.48PVISYY6 pKa = 9.12SLRR9 pKa = 11.84AHH11 pKa = 6.76GEE13 pKa = 3.69GHH15 pKa = 6.77LRR17 pKa = 11.84FSNGIEE23 pKa = 3.65QLGSNEE29 pKa = 3.82TMHH32 pKa = 6.05QKK34 pKa = 10.16VYY36 pKa = 10.7RR37 pKa = 11.84RR38 pKa = 11.84ALNKK42 pKa = 10.17VGDD45 pKa = 3.66KK46 pKa = 10.82AYY48 pKa = 8.65TQVIRR53 pKa = 11.84ALTKK57 pKa = 10.31QMGLKK62 pKa = 9.75RR63 pKa = 11.84RR64 pKa = 11.84QVLTYY69 pKa = 10.28GGVRR73 pKa = 11.84PVRR76 pKa = 11.84ANLTRR81 pKa = 11.84QDD83 pKa = 3.52FQIYY87 pKa = 7.97STGAEE92 pKa = 3.99VPLRR96 pKa = 11.84EE97 pKa = 4.11FSAVQFSFGVRR108 pKa = 11.84ARR110 pKa = 11.84PWGKK114 pKa = 7.71STRR117 pKa = 11.84FSGMFIHH124 pKa = 7.59AGRR127 pKa = 11.84WNSGKK132 pKa = 10.17DD133 pKa = 3.24VAQGHH138 pKa = 5.19VFQRR142 pKa = 11.84VTSSSLPIEE151 pKa = 4.33KK152 pKa = 10.13QFGPSVPAEE161 pKa = 4.1MVKK164 pKa = 10.8GEE166 pKa = 4.31SEE168 pKa = 3.9AAFNRR173 pKa = 11.84MADD176 pKa = 3.57QLPDD180 pKa = 4.64RR181 pKa = 11.84IAHH184 pKa = 6.95EE185 pKa = 4.0IAQITRR191 pKa = 11.84GVVSS195 pKa = 3.87

Molecular weight: 21.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

15425

39

829

205.7

22.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.802 ± 0.495

0.888 ± 0.103

6.139 ± 0.244

5.718 ± 0.296

2.626 ± 0.137

9.232 ± 0.532

1.854 ± 0.153

4.947 ± 0.175

3.28 ± 0.21

8.188 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.723 ± 0.165

2.444 ± 0.157

5.186 ± 0.308

3.501 ± 0.184

7.734 ± 0.284

5.199 ± 0.196

5.874 ± 0.218

6.801 ± 0.233

1.861 ± 0.149

2.003 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski