Amino acid dipepetide frequency for Clostridium phage phiCT9441A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.666AlaAla: 1.666 ± 0.474
0.29AlaCys: 0.29 ± 0.158
2.752AlaAsp: 2.752 ± 0.388
3.911AlaGlu: 3.911 ± 0.565
2.463AlaPhe: 2.463 ± 0.418
2.535AlaGly: 2.535 ± 0.446
0.797AlaHis: 0.797 ± 0.217
4.491AlaIle: 4.491 ± 0.583
5.36AlaLys: 5.36 ± 0.727
5.143AlaLeu: 5.143 ± 0.629
1.159AlaMet: 1.159 ± 0.325
3.115AlaAsn: 3.115 ± 0.436
0.724AlaPro: 0.724 ± 0.194
1.449AlaGln: 1.449 ± 0.353
1.738AlaArg: 1.738 ± 0.286
2.897AlaSer: 2.897 ± 0.711
2.897AlaThr: 2.897 ± 0.426
2.245AlaVal: 2.245 ± 0.558
0.652AlaTrp: 0.652 ± 0.224
1.594AlaTyr: 1.594 ± 0.346
0.0AlaXaa: 0.0 ± 0.0
Cys
0.145CysAla: 0.145 ± 0.125
0.0CysCys: 0.0 ± 0.0
0.435CysAsp: 0.435 ± 0.183
0.797CysGlu: 0.797 ± 0.242
0.362CysPhe: 0.362 ± 0.136
0.579CysGly: 0.579 ± 0.299
0.145CysHis: 0.145 ± 0.105
0.797CysIle: 0.797 ± 0.266
1.086CysLys: 1.086 ± 0.305
0.579CysLeu: 0.579 ± 0.207
0.145CysMet: 0.145 ± 0.129
0.724CysAsn: 0.724 ± 0.255
0.145CysPro: 0.145 ± 0.108
0.145CysGln: 0.145 ± 0.101
0.507CysArg: 0.507 ± 0.219
0.652CysSer: 0.652 ± 0.288
0.362CysThr: 0.362 ± 0.187
0.362CysVal: 0.362 ± 0.15
0.0CysTrp: 0.0 ± 0.0
0.362CysTyr: 0.362 ± 0.139
0.0CysXaa: 0.0 ± 0.0
Asp
1.811AspAla: 1.811 ± 0.434
0.362AspCys: 0.362 ± 0.151
3.042AspAsp: 3.042 ± 0.499
4.129AspGlu: 4.129 ± 0.594
2.752AspPhe: 2.752 ± 0.473
3.259AspGly: 3.259 ± 0.381
0.652AspHis: 0.652 ± 0.208
6.881AspIle: 6.881 ± 0.747
7.678AspLys: 7.678 ± 0.714
5.215AspLeu: 5.215 ± 0.598
1.521AspMet: 1.521 ± 0.326
4.346AspAsn: 4.346 ± 0.574
0.942AspPro: 0.942 ± 0.28
1.376AspGln: 1.376 ± 0.292
2.173AspArg: 2.173 ± 0.361
2.897AspSer: 2.897 ± 0.462
3.622AspThr: 3.622 ± 0.416
3.766AspVal: 3.766 ± 0.498
0.579AspTrp: 0.579 ± 0.179
2.608AspTyr: 2.608 ± 0.41
0.0AspXaa: 0.0 ± 0.0
Glu
3.404GluAla: 3.404 ± 0.44
0.652GluCys: 0.652 ± 0.236
5.215GluAsp: 5.215 ± 0.63
8.909GluGlu: 8.909 ± 1.021
3.911GluPhe: 3.911 ± 0.595
4.274GluGly: 4.274 ± 0.444
1.376GluHis: 1.376 ± 0.305
7.605GluIle: 7.605 ± 0.812
9.126GluLys: 9.126 ± 0.874
8.402GluLeu: 8.402 ± 0.854
1.738GluMet: 1.738 ± 0.408
4.781GluAsn: 4.781 ± 0.569
1.666GluPro: 1.666 ± 0.354
3.332GluGln: 3.332 ± 0.402
3.404GluArg: 3.404 ± 0.663
4.201GluSer: 4.201 ± 0.627
4.056GluThr: 4.056 ± 0.607
5.795GluVal: 5.795 ± 0.772
1.086GluTrp: 1.086 ± 0.247
4.274GluTyr: 4.274 ± 0.512
0.0GluXaa: 0.0 ± 0.0
Phe
1.304PheAla: 1.304 ± 0.293
0.217PheCys: 0.217 ± 0.132
2.608PheAsp: 2.608 ± 0.414
2.97PheGlu: 2.97 ± 0.475
1.594PhePhe: 1.594 ± 0.367
2.245PheGly: 2.245 ± 0.551
0.435PheHis: 0.435 ± 0.178
3.622PheIle: 3.622 ± 0.569
5.143PheLys: 5.143 ± 0.568
3.332PheLeu: 3.332 ± 0.581
0.724PheMet: 0.724 ± 0.246
3.694PheAsn: 3.694 ± 0.536
0.942PhePro: 0.942 ± 0.242
1.014PheGln: 1.014 ± 0.247
1.014PheArg: 1.014 ± 0.238
2.318PheSer: 2.318 ± 0.366
1.956PheThr: 1.956 ± 0.344
1.159PheVal: 1.159 ± 0.241
0.217PheTrp: 0.217 ± 0.119
1.811PheTyr: 1.811 ± 0.388
0.0PheXaa: 0.0 ± 0.0
Gly
2.897GlyAla: 2.897 ± 0.571
0.579GlyCys: 0.579 ± 0.178
3.259GlyAsp: 3.259 ± 0.503
3.404GlyGlu: 3.404 ± 0.599
2.101GlyPhe: 2.101 ± 0.45
2.825GlyGly: 2.825 ± 0.549
0.579GlyHis: 0.579 ± 0.178
5.432GlyIle: 5.432 ± 0.633
5.795GlyLys: 5.795 ± 0.622
4.056GlyLeu: 4.056 ± 0.825
1.521GlyMet: 1.521 ± 0.313
4.201GlyAsn: 4.201 ± 0.492
0.797GlyPro: 0.797 ± 0.214
1.449GlyGln: 1.449 ± 0.308
1.738GlyArg: 1.738 ± 0.406
2.897GlySer: 2.897 ± 0.582
2.897GlyThr: 2.897 ± 0.609
3.766GlyVal: 3.766 ± 0.471
1.014GlyTrp: 1.014 ± 0.29
2.463GlyTyr: 2.463 ± 0.504
0.0GlyXaa: 0.0 ± 0.0
His
0.435HisAla: 0.435 ± 0.19
0.0HisCys: 0.0 ± 0.0
0.797HisAsp: 0.797 ± 0.214
0.942HisGlu: 0.942 ± 0.241
0.507HisPhe: 0.507 ± 0.197
0.869HisGly: 0.869 ± 0.243
0.145HisHis: 0.145 ± 0.115
1.231HisIle: 1.231 ± 0.261
1.159HisLys: 1.159 ± 0.32
1.159HisLeu: 1.159 ± 0.272
0.217HisMet: 0.217 ± 0.111
0.579HisAsn: 0.579 ± 0.224
0.579HisPro: 0.579 ± 0.194
0.217HisGln: 0.217 ± 0.12
0.507HisArg: 0.507 ± 0.191
0.797HisSer: 0.797 ± 0.256
0.507HisThr: 0.507 ± 0.182
0.579HisVal: 0.579 ± 0.181
0.362HisTrp: 0.362 ± 0.152
0.362HisTyr: 0.362 ± 0.168
0.0HisXaa: 0.0 ± 0.0
Ile
4.418IleAla: 4.418 ± 0.477
1.014IleCys: 1.014 ± 0.286
5.867IleAsp: 5.867 ± 0.597
9.633IleGlu: 9.633 ± 0.83
2.825IlePhe: 2.825 ± 0.43
3.984IleGly: 3.984 ± 0.586
0.797IleHis: 0.797 ± 0.26
8.112IleIle: 8.112 ± 0.79
10.865IleLys: 10.865 ± 1.007
7.605IleLeu: 7.605 ± 0.797
1.883IleMet: 1.883 ± 0.373
8.547IleAsn: 8.547 ± 0.952
2.752IlePro: 2.752 ± 0.461
2.752IleGln: 2.752 ± 0.477
3.259IleArg: 3.259 ± 0.456
5.65IleSer: 5.65 ± 0.589
4.781IleThr: 4.781 ± 0.669
4.346IleVal: 4.346 ± 0.605
0.652IleTrp: 0.652 ± 0.216
3.694IleTyr: 3.694 ± 0.546
0.0IleXaa: 0.0 ± 0.0
Lys
6.084LysAla: 6.084 ± 0.724
1.304LysCys: 1.304 ± 0.341
7.098LysAsp: 7.098 ± 0.803
11.589LysGlu: 11.589 ± 1.076
3.839LysPhe: 3.839 ± 0.474
5.143LysGly: 5.143 ± 0.75
1.811LysHis: 1.811 ± 0.343
10.358LysIle: 10.358 ± 1.022
11.082LysLys: 11.082 ± 0.787
8.764LysLeu: 8.764 ± 0.939
4.346LysMet: 4.346 ± 0.577
8.185LysAsn: 8.185 ± 0.821
2.318LysPro: 2.318 ± 0.426
4.853LysGln: 4.853 ± 0.502
4.201LysArg: 4.201 ± 0.691
5.65LysSer: 5.65 ± 0.676
5.215LysThr: 5.215 ± 0.543
5.795LysVal: 5.795 ± 0.701
1.231LysTrp: 1.231 ± 0.302
4.636LysTyr: 4.636 ± 0.696
0.0LysXaa: 0.0 ± 0.0
Leu
5.432LeuAla: 5.432 ± 0.628
1.086LeuCys: 1.086 ± 0.323
4.925LeuAsp: 4.925 ± 0.628
7.533LeuGlu: 7.533 ± 0.755
2.97LeuPhe: 2.97 ± 0.4
5.215LeuGly: 5.215 ± 0.661
0.797LeuHis: 0.797 ± 0.267
6.302LeuIle: 6.302 ± 0.673
11.299LeuLys: 11.299 ± 0.929
6.591LeuLeu: 6.591 ± 0.773
2.245LeuMet: 2.245 ± 0.398
6.157LeuAsn: 6.157 ± 0.65
1.738LeuPro: 1.738 ± 0.345
2.535LeuGln: 2.535 ± 0.407
2.245LeuArg: 2.245 ± 0.353
4.418LeuSer: 4.418 ± 0.514
4.274LeuThr: 4.274 ± 0.58
3.187LeuVal: 3.187 ± 0.459
0.797LeuTrp: 0.797 ± 0.201
3.115LeuTyr: 3.115 ± 0.515
0.0LeuXaa: 0.0 ± 0.0
Met
1.738MetAla: 1.738 ± 0.395
0.072MetCys: 0.072 ± 0.063
1.666MetAsp: 1.666 ± 0.335
2.752MetGlu: 2.752 ± 0.441
0.797MetPhe: 0.797 ± 0.23
1.086MetGly: 1.086 ± 0.241
0.435MetHis: 0.435 ± 0.174
1.521MetIle: 1.521 ± 0.32
3.042MetLys: 3.042 ± 0.493
2.68MetLeu: 2.68 ± 0.453
0.362MetMet: 0.362 ± 0.157
2.101MetAsn: 2.101 ± 0.369
0.797MetPro: 0.797 ± 0.25
0.942MetGln: 0.942 ± 0.302
0.579MetArg: 0.579 ± 0.214
1.738MetSer: 1.738 ± 0.345
1.086MetThr: 1.086 ± 0.337
0.797MetVal: 0.797 ± 0.256
0.362MetTrp: 0.362 ± 0.155
1.086MetTyr: 1.086 ± 0.324
0.0MetXaa: 0.0 ± 0.0
Asn
3.259AsnAla: 3.259 ± 0.741
0.435AsnCys: 0.435 ± 0.168
3.187AsnAsp: 3.187 ± 0.424
5.215AsnGlu: 5.215 ± 0.651
2.752AsnPhe: 2.752 ± 0.336
4.563AsnGly: 4.563 ± 0.573
0.579AsnHis: 0.579 ± 0.213
7.75AsnIle: 7.75 ± 0.866
10.068AsnLys: 10.068 ± 0.911
5.795AsnLeu: 5.795 ± 0.626
2.028AsnMet: 2.028 ± 0.348
6.736AsnAsn: 6.736 ± 0.642
2.173AsnPro: 2.173 ± 0.467
1.666AsnGln: 1.666 ± 0.364
2.68AsnArg: 2.68 ± 0.354
4.491AsnSer: 4.491 ± 0.836
4.056AsnThr: 4.056 ± 0.547
2.97AsnVal: 2.97 ± 0.431
1.231AsnTrp: 1.231 ± 0.348
2.825AsnTyr: 2.825 ± 0.442
0.0AsnXaa: 0.0 ± 0.0
Pro
0.869ProAla: 0.869 ± 0.245
0.145ProCys: 0.145 ± 0.095
1.521ProAsp: 1.521 ± 0.329
2.608ProGlu: 2.608 ± 0.39
1.086ProPhe: 1.086 ± 0.33
1.086ProGly: 1.086 ± 0.339
0.145ProHis: 0.145 ± 0.096
2.028ProIle: 2.028 ± 0.445
2.173ProLys: 2.173 ± 0.42
2.028ProLeu: 2.028 ± 0.458
0.652ProMet: 0.652 ± 0.29
1.159ProAsn: 1.159 ± 0.206
1.014ProPro: 1.014 ± 0.3
0.724ProGln: 0.724 ± 0.219
0.579ProArg: 0.579 ± 0.201
1.231ProSer: 1.231 ± 0.297
1.376ProThr: 1.376 ± 0.323
1.594ProVal: 1.594 ± 0.43
0.362ProTrp: 0.362 ± 0.129
0.869ProTyr: 0.869 ± 0.279
0.0ProXaa: 0.0 ± 0.0
Gln
1.738GlnAla: 1.738 ± 0.356
0.29GlnCys: 0.29 ± 0.145
1.449GlnAsp: 1.449 ± 0.327
2.825GlnGlu: 2.825 ± 0.408
1.304GlnPhe: 1.304 ± 0.273
2.101GlnGly: 2.101 ± 0.392
0.29GlnHis: 0.29 ± 0.191
3.187GlnIle: 3.187 ± 0.534
2.752GlnLys: 2.752 ± 0.386
2.535GlnLeu: 2.535 ± 0.344
0.652GlnMet: 0.652 ± 0.235
2.028GlnAsn: 2.028 ± 0.311
0.797GlnPro: 0.797 ± 0.204
1.304GlnGln: 1.304 ± 0.274
1.376GlnArg: 1.376 ± 0.271
2.101GlnSer: 2.101 ± 0.41
1.449GlnThr: 1.449 ± 0.283
1.594GlnVal: 1.594 ± 0.41
0.145GlnTrp: 0.145 ± 0.091
1.231GlnTyr: 1.231 ± 0.285
0.0GlnXaa: 0.0 ± 0.0
Arg
1.738ArgAla: 1.738 ± 0.302
0.29ArgCys: 0.29 ± 0.152
2.101ArgAsp: 2.101 ± 0.368
3.187ArgGlu: 3.187 ± 0.515
0.942ArgPhe: 0.942 ± 0.27
1.738ArgGly: 1.738 ± 0.322
0.217ArgHis: 0.217 ± 0.16
3.839ArgIle: 3.839 ± 0.56
3.766ArgLys: 3.766 ± 0.568
2.463ArgLeu: 2.463 ± 0.393
1.666ArgMet: 1.666 ± 0.384
2.173ArgAsn: 2.173 ± 0.345
0.797ArgPro: 0.797 ± 0.241
1.086ArgGln: 1.086 ± 0.252
1.594ArgArg: 1.594 ± 0.389
1.883ArgSer: 1.883 ± 0.338
1.738ArgThr: 1.738 ± 0.402
2.028ArgVal: 2.028 ± 0.366
0.652ArgTrp: 0.652 ± 0.241
2.028ArgTyr: 2.028 ± 0.334
0.0ArgXaa: 0.0 ± 0.0
Ser
2.608SerAla: 2.608 ± 0.652
0.579SerCys: 0.579 ± 0.206
3.549SerAsp: 3.549 ± 0.548
4.129SerGlu: 4.129 ± 0.717
2.608SerPhe: 2.608 ± 0.389
3.694SerGly: 3.694 ± 0.552
0.869SerHis: 0.869 ± 0.233
5.65SerIle: 5.65 ± 0.707
6.591SerLys: 6.591 ± 0.752
3.332SerLeu: 3.332 ± 0.477
1.449SerMet: 1.449 ± 0.287
4.491SerAsn: 4.491 ± 0.592
1.159SerPro: 1.159 ± 0.348
1.521SerGln: 1.521 ± 0.31
2.608SerArg: 2.608 ± 0.417
2.825SerSer: 2.825 ± 0.66
2.463SerThr: 2.463 ± 0.442
2.825SerVal: 2.825 ± 0.419
0.217SerTrp: 0.217 ± 0.125
3.115SerTyr: 3.115 ± 0.568
0.0SerXaa: 0.0 ± 0.0
Thr
2.608ThrAla: 2.608 ± 0.444
0.435ThrCys: 0.435 ± 0.173
2.97ThrAsp: 2.97 ± 0.438
3.984ThrGlu: 3.984 ± 0.624
2.173ThrPhe: 2.173 ± 0.431
3.187ThrGly: 3.187 ± 0.475
0.797ThrHis: 0.797 ± 0.222
4.636ThrIle: 4.636 ± 0.668
4.563ThrLys: 4.563 ± 0.51
5.143ThrLeu: 5.143 ± 0.623
0.797ThrMet: 0.797 ± 0.276
3.549ThrAsn: 3.549 ± 0.479
1.666ThrPro: 1.666 ± 0.367
1.304ThrGln: 1.304 ± 0.374
1.956ThrArg: 1.956 ± 0.36
3.694ThrSer: 3.694 ± 0.483
3.187ThrThr: 3.187 ± 0.52
2.173ThrVal: 2.173 ± 0.448
0.724ThrTrp: 0.724 ± 0.268
2.245ThrTyr: 2.245 ± 0.41
0.0ThrXaa: 0.0 ± 0.0
Val
3.404ValAla: 3.404 ± 0.64
0.145ValCys: 0.145 ± 0.091
4.056ValAsp: 4.056 ± 0.533
3.911ValGlu: 3.911 ± 0.679
1.811ValPhe: 1.811 ± 0.336
2.173ValGly: 2.173 ± 0.344
0.362ValHis: 0.362 ± 0.195
5.215ValIle: 5.215 ± 0.598
5.432ValLys: 5.432 ± 0.56
3.984ValLeu: 3.984 ± 0.537
1.304ValMet: 1.304 ± 0.276
3.549ValAsn: 3.549 ± 0.373
1.231ValPro: 1.231 ± 0.266
1.811ValGln: 1.811 ± 0.316
1.449ValArg: 1.449 ± 0.266
2.68ValSer: 2.68 ± 0.45
2.68ValThr: 2.68 ± 0.39
2.752ValVal: 2.752 ± 0.424
0.435ValTrp: 0.435 ± 0.17
2.245ValTyr: 2.245 ± 0.354
0.0ValXaa: 0.0 ± 0.0
Trp
0.435TrpAla: 0.435 ± 0.192
0.072TrpCys: 0.072 ± 0.072
0.435TrpAsp: 0.435 ± 0.199
0.942TrpGlu: 0.942 ± 0.27
0.435TrpPhe: 0.435 ± 0.176
0.724TrpGly: 0.724 ± 0.189
0.145TrpHis: 0.145 ± 0.11
0.942TrpIle: 0.942 ± 0.3
0.724TrpLys: 0.724 ± 0.206
0.724TrpLeu: 0.724 ± 0.224
0.29TrpMet: 0.29 ± 0.143
1.159TrpAsn: 1.159 ± 0.285
0.0TrpPro: 0.0 ± 0.0
0.652TrpGln: 0.652 ± 0.206
0.724TrpArg: 0.724 ± 0.223
0.724TrpSer: 0.724 ± 0.258
0.942TrpThr: 0.942 ± 0.269
0.724TrpVal: 0.724 ± 0.276
0.0TrpTrp: 0.0 ± 0.0
0.435TrpTyr: 0.435 ± 0.183
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.883TyrAla: 1.883 ± 0.469
0.435TyrCys: 0.435 ± 0.202
2.68TyrAsp: 2.68 ± 0.469
3.839TyrGlu: 3.839 ± 0.511
1.086TyrPhe: 1.086 ± 0.287
2.39TyrGly: 2.39 ± 0.425
0.579TyrHis: 0.579 ± 0.194
3.839TyrIle: 3.839 ± 0.687
5.795TyrLys: 5.795 ± 0.634
3.332TyrLeu: 3.332 ± 0.539
0.869TyrMet: 0.869 ± 0.248
3.187TyrAsn: 3.187 ± 0.542
1.014TyrPro: 1.014 ± 0.293
1.014TyrGln: 1.014 ± 0.232
1.594TyrArg: 1.594 ± 0.359
2.535TyrSer: 2.535 ± 0.422
2.245TyrThr: 2.245 ± 0.411
2.173TyrVal: 2.173 ± 0.494
0.507TyrTrp: 0.507 ± 0.224
1.883TyrTyr: 1.883 ± 0.368
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 77 proteins (13807 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski