Amino acid dipepetide frequency for Lysinibacillus sp. F5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.208AlaAla: 6.208 ± 0.101	0.657AlaCys: 0.657 ± 0.024	3.606AlaAsp: 3.606 ± 0.057	4.967AlaGlu: 4.967 ± 0.071	3.568AlaPhe: 3.568 ± 0.056	5.199AlaGly: 5.199 ± 0.077	1.499AlaHis: 1.499 ± 0.036	6.827AlaIle: 6.827 ± 0.08	4.946AlaLys: 4.946 ± 0.062	8.041AlaLeu: 8.041 ± 0.082	2.302AlaMet: 2.302 ± 0.047	3.124AlaAsn: 3.124 ± 0.053	2.168AlaPro: 2.168 ± 0.046	3.289AlaGln: 3.289 ± 0.048	2.756AlaArg: 2.756 ± 0.048	4.469AlaSer: 4.469 ± 0.06	4.519AlaThr: 4.519 ± 0.057	5.582AlaVal: 5.582 ± 0.064	0.651AlaTrp: 0.651 ± 0.028	2.616AlaTyr: 2.616 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.475CysAla: 0.475 ± 0.021	0.103CysCys: 0.103 ± 0.01	0.382CysAsp: 0.382 ± 0.018	0.463CysGlu: 0.463 ± 0.023	0.315CysPhe: 0.315 ± 0.018	0.653CysGly: 0.653 ± 0.028	0.215CysHis: 0.215 ± 0.014	0.577CysIle: 0.577 ± 0.02	0.313CysLys: 0.313 ± 0.018	0.746CysLeu: 0.746 ± 0.027	0.173CysMet: 0.173 ± 0.011	0.266CysAsn: 0.266 ± 0.016	0.325CysPro: 0.325 ± 0.017	0.287CysGln: 0.287 ± 0.015	0.249CysArg: 0.249 ± 0.013	0.482CysSer: 0.482 ± 0.02	0.437CysThr: 0.437 ± 0.017	0.45CysVal: 0.45 ± 0.021	0.079CysTrp: 0.079 ± 0.008	0.273CysTyr: 0.273 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.732AspAla: 3.732 ± 0.059	0.352AspCys: 0.352 ± 0.019	2.373AspAsp: 2.373 ± 0.044	4.155AspGlu: 4.155 ± 0.054	2.453AspPhe: 2.453 ± 0.049	3.308AspGly: 3.308 ± 0.056	1.088AspHis: 1.088 ± 0.033	4.238AspIle: 4.238 ± 0.062	2.937AspLys: 2.937 ± 0.053	4.671AspLeu: 4.671 ± 0.07	1.381AspMet: 1.381 ± 0.033	1.903AspAsn: 1.903 ± 0.04	1.663AspPro: 1.663 ± 0.038	1.75AspGln: 1.75 ± 0.036	1.881AspArg: 1.881 ± 0.042	2.408AspSer: 2.408 ± 0.043	2.496AspThr: 2.496 ± 0.041	3.871AspVal: 3.871 ± 0.057	0.589AspTrp: 0.589 ± 0.019	2.271AspTyr: 2.271 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	5.776GluAla: 5.776 ± 0.064	0.376GluCys: 0.376 ± 0.017	3.642GluAsp: 3.642 ± 0.059	6.263GluGlu: 6.263 ± 0.081	2.338GluPhe: 2.338 ± 0.049	4.033GluGly: 4.033 ± 0.06	1.628GluHis: 1.628 ± 0.037	5.299GluIle: 5.299 ± 0.064	5.652GluLys: 5.652 ± 0.071	6.878GluLeu: 6.878 ± 0.076	2.187GluMet: 2.187 ± 0.04	3.237GluAsn: 3.237 ± 0.058	2.0GluPro: 2.0 ± 0.039	4.24GluGln: 4.24 ± 0.078	3.34GluArg: 3.34 ± 0.065	3.143GluSer: 3.143 ± 0.048	3.658GluThr: 3.658 ± 0.062	4.874GluVal: 4.874 ± 0.073	0.782GluTrp: 0.782 ± 0.023	1.982GluTyr: 1.982 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	3.262PheAla: 3.262 ± 0.052	0.34PheCys: 0.34 ± 0.018	2.392PheAsp: 2.392 ± 0.048	2.877PheGlu: 2.877 ± 0.047	2.263PhePhe: 2.263 ± 0.053	3.102PheGly: 3.102 ± 0.061	0.985PheHis: 0.985 ± 0.029	3.976PheIle: 3.976 ± 0.069	2.362PheLys: 2.362 ± 0.043	4.427PheLeu: 4.427 ± 0.076	1.269PheMet: 1.269 ± 0.034	1.971PheAsn: 1.971 ± 0.04	1.509PhePro: 1.509 ± 0.031	1.748PheGln: 1.748 ± 0.037	1.36PheArg: 1.36 ± 0.032	3.128PheSer: 3.128 ± 0.052	2.917PheThr: 2.917 ± 0.056	3.29PheVal: 3.29 ± 0.053	0.456PheTrp: 0.456 ± 0.018	1.783PheTyr: 1.783 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	4.857GlyAla: 4.857 ± 0.079	0.588GlyCys: 0.588 ± 0.023	3.135GlyAsp: 3.135 ± 0.048	4.186GlyGlu: 4.186 ± 0.061	3.191GlyPhe: 3.191 ± 0.058	4.45GlyGly: 4.45 ± 0.086	1.431GlyHis: 1.431 ± 0.038	5.622GlyIle: 5.622 ± 0.073	4.361GlyLys: 4.361 ± 0.059	6.232GlyLeu: 6.232 ± 0.076	1.941GlyMet: 1.941 ± 0.036	2.47GlyAsn: 2.47 ± 0.049	1.566GlyPro: 1.566 ± 0.038	2.546GlyGln: 2.546 ± 0.052	2.396GlyArg: 2.396 ± 0.044	3.624GlySer: 3.624 ± 0.059	3.94GlyThr: 3.94 ± 0.066	4.777GlyVal: 4.777 ± 0.064	0.723GlyTrp: 0.723 ± 0.024	2.805GlyTyr: 2.805 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	1.558HisAla: 1.558 ± 0.039	0.214HisCys: 0.214 ± 0.014	1.059HisAsp: 1.059 ± 0.031	1.485HisGlu: 1.485 ± 0.037	1.201HisPhe: 1.201 ± 0.034	1.373HisGly: 1.373 ± 0.034	0.719HisHis: 0.719 ± 0.027	1.667HisIle: 1.667 ± 0.039	1.029HisLys: 1.029 ± 0.028	2.276HisLeu: 2.276 ± 0.048	0.568HisMet: 0.568 ± 0.021	0.836HisAsn: 0.836 ± 0.023	1.054HisPro: 1.054 ± 0.03	1.021HisGln: 1.021 ± 0.031	0.852HisArg: 0.852 ± 0.026	1.126HisSer: 1.126 ± 0.025	1.154HisThr: 1.154 ± 0.036	1.506HisVal: 1.506 ± 0.034	0.253HisTrp: 0.253 ± 0.015	1.036HisTyr: 1.036 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	6.774IleAla: 6.774 ± 0.083	0.667IleCys: 0.667 ± 0.021	4.279IleAsp: 4.279 ± 0.061	5.934IleGlu: 5.934 ± 0.078	3.476IlePhe: 3.476 ± 0.057	5.869IleGly: 5.869 ± 0.084	1.784IleHis: 1.784 ± 0.037	6.17IleIle: 6.17 ± 0.098	3.899IleLys: 3.899 ± 0.061	7.416IleLeu: 7.416 ± 0.087	1.93IleMet: 1.93 ± 0.034	3.057IleAsn: 3.057 ± 0.055	3.139IlePro: 3.139 ± 0.058	3.486IleGln: 3.486 ± 0.055	3.068IleArg: 3.068 ± 0.062	4.723IleSer: 4.723 ± 0.07	4.489IleThr: 4.489 ± 0.067	6.125IleVal: 6.125 ± 0.075	0.682IleTrp: 0.682 ± 0.024	2.519IleTyr: 2.519 ± 0.05	0.0IleXaa: 0.0 ± 0.0
Lys	4.55LysAla: 4.55 ± 0.064	0.32LysCys: 0.32 ± 0.019	3.416LysAsp: 3.416 ± 0.06	5.698LysGlu: 5.698 ± 0.07	1.912LysPhe: 1.912 ± 0.042	3.97LysGly: 3.97 ± 0.057	1.279LysHis: 1.279 ± 0.033	4.588LysIle: 4.588 ± 0.076	4.929LysLys: 4.929 ± 0.076	5.664LysLeu: 5.664 ± 0.061	2.238LysMet: 2.238 ± 0.038	3.121LysAsn: 3.121 ± 0.054	2.094LysPro: 2.094 ± 0.042	3.052LysGln: 3.052 ± 0.06	2.804LysArg: 2.804 ± 0.05	3.218LysSer: 3.218 ± 0.054	3.363LysThr: 3.363 ± 0.047	4.248LysVal: 4.248 ± 0.059	0.789LysTrp: 0.789 ± 0.027	2.097LysTyr: 2.097 ± 0.043	0.0LysXaa: 0.0 ± 0.0
Leu	8.485LeuAla: 8.485 ± 0.087	0.752LeuCys: 0.752 ± 0.027	4.622LeuAsp: 4.622 ± 0.064	6.454LeuGlu: 6.454 ± 0.082	4.852LeuPhe: 4.852 ± 0.079	6.167LeuGly: 6.167 ± 0.084	2.171LeuHis: 2.171 ± 0.047	6.879LeuIle: 6.879 ± 0.099	6.029LeuLys: 6.029 ± 0.072	10.684LeuLeu: 10.684 ± 0.126	2.701LeuMet: 2.701 ± 0.053	4.115LeuAsn: 4.115 ± 0.061	4.015LeuPro: 4.015 ± 0.056	4.797LeuGln: 4.797 ± 0.085	3.602LeuArg: 3.602 ± 0.062	6.37LeuSer: 6.37 ± 0.075	6.163LeuThr: 6.163 ± 0.064	6.456LeuVal: 6.456 ± 0.075	0.831LeuTrp: 0.831 ± 0.026	3.312LeuTyr: 3.312 ± 0.057	0.0LeuXaa: 0.0 ± 0.0
Met	2.199MetAla: 2.199 ± 0.041	0.162MetCys: 0.162 ± 0.011	1.481MetAsp: 1.481 ± 0.031	1.851MetGlu: 1.851 ± 0.038	1.065MetPhe: 1.065 ± 0.032	1.67MetGly: 1.67 ± 0.038	0.566MetHis: 0.566 ± 0.021	2.136MetIle: 2.136 ± 0.042	2.436MetLys: 2.436 ± 0.046	2.835MetLeu: 2.835 ± 0.047	0.92MetMet: 0.92 ± 0.032	1.526MetAsn: 1.526 ± 0.036	1.066MetPro: 1.066 ± 0.026	1.251MetGln: 1.251 ± 0.033	1.13MetArg: 1.13 ± 0.033	1.743MetSer: 1.743 ± 0.039	2.021MetThr: 2.021 ± 0.04	1.684MetVal: 1.684 ± 0.036	0.197MetTrp: 0.197 ± 0.013	0.847MetTyr: 0.847 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	3.213AsnAla: 3.213 ± 0.048	0.323AsnCys: 0.323 ± 0.016	2.257AsnAsp: 2.257 ± 0.046	3.313AsnGlu: 3.313 ± 0.059	1.688AsnPhe: 1.688 ± 0.038	3.185AsnGly: 3.185 ± 0.059	0.933AsnHis: 0.933 ± 0.023	3.323AsnIle: 3.323 ± 0.057	2.671AsnLys: 2.671 ± 0.056	3.726AsnLeu: 3.726 ± 0.055	1.166AsnMet: 1.166 ± 0.028	1.951AsnAsn: 1.951 ± 0.048	1.993AsnPro: 1.993 ± 0.043	1.733AsnGln: 1.733 ± 0.044	1.803AsnArg: 1.803 ± 0.036	2.152AsnSer: 2.152 ± 0.052	2.324AsnThr: 2.324 ± 0.042	3.014AsnVal: 3.014 ± 0.048	0.449AsnTrp: 0.449 ± 0.02	1.621AsnTyr: 1.621 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	2.372ProAla: 2.372 ± 0.049	0.207ProCys: 0.207 ± 0.013	1.715ProAsp: 1.715 ± 0.04	2.452ProGlu: 2.452 ± 0.044	1.997ProPhe: 1.997 ± 0.042	1.912ProGly: 1.912 ± 0.04	0.847ProHis: 0.847 ± 0.026	3.004ProIle: 3.004 ± 0.049	2.069ProLys: 2.069 ± 0.038	3.435ProLeu: 3.435 ± 0.056	0.877ProMet: 0.877 ± 0.033	1.699ProAsn: 1.699 ± 0.036	0.921ProPro: 0.921 ± 0.034	1.314ProGln: 1.314 ± 0.033	0.973ProArg: 0.973 ± 0.025	2.202ProSer: 2.202 ± 0.043	2.262ProThr: 2.262 ± 0.045	2.6ProVal: 2.6 ± 0.043	0.333ProTrp: 0.333 ± 0.018	1.495ProTyr: 1.495 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	3.546GlnAla: 3.546 ± 0.056	0.216GlnCys: 0.216 ± 0.014	2.006GlnAsp: 2.006 ± 0.037	3.313GlnGlu: 3.313 ± 0.058	1.831GlnPhe: 1.831 ± 0.042	2.406GlnGly: 2.406 ± 0.047	1.167GlnHis: 1.167 ± 0.032	2.932GlnIle: 2.932 ± 0.053	2.831GlnLys: 2.831 ± 0.049	5.034GlnLeu: 5.034 ± 0.075	1.378GlnMet: 1.378 ± 0.029	1.755GlnAsn: 1.755 ± 0.042	1.454GlnPro: 1.454 ± 0.035	3.126GlnGln: 3.126 ± 0.07	1.743GlnArg: 1.743 ± 0.04	2.453GlnSer: 2.453 ± 0.052	2.398GlnThr: 2.398 ± 0.049	2.697GlnVal: 2.697 ± 0.045	0.462GlnTrp: 0.462 ± 0.02	1.591GlnTyr: 1.591 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	2.727ArgAla: 2.727 ± 0.051	0.245ArgCys: 0.245 ± 0.015	1.941ArgAsp: 1.941 ± 0.038	2.858ArgGlu: 2.858 ± 0.051	1.856ArgPhe: 1.856 ± 0.037	2.161ArgGly: 2.161 ± 0.044	0.852ArgHis: 0.852 ± 0.024	2.907ArgIle: 2.907 ± 0.049	2.745ArgLys: 2.745 ± 0.046	3.922ArgLeu: 3.922 ± 0.06	1.163ArgMet: 1.163 ± 0.032	1.621ArgAsn: 1.621 ± 0.037	1.185ArgPro: 1.185 ± 0.03	1.764ArgGln: 1.764 ± 0.037	1.599ArgArg: 1.599 ± 0.047	1.977ArgSer: 1.977 ± 0.039	2.029ArgThr: 2.029 ± 0.039	2.591ArgVal: 2.591 ± 0.046	0.385ArgTrp: 0.385 ± 0.016	1.499ArgTyr: 1.499 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	3.939SerAla: 3.939 ± 0.064	0.385SerCys: 0.385 ± 0.019	2.429SerAsp: 2.429 ± 0.049	3.414SerGlu: 3.414 ± 0.057	3.232SerPhe: 3.232 ± 0.054	3.885SerGly: 3.885 ± 0.06	1.197SerHis: 1.197 ± 0.032	5.202SerIle: 5.202 ± 0.068	3.535SerLys: 3.535 ± 0.056	5.967SerLeu: 5.967 ± 0.066	1.717SerMet: 1.717 ± 0.039	2.518SerAsn: 2.518 ± 0.047	2.021SerPro: 2.021 ± 0.043	2.094SerGln: 2.094 ± 0.036	1.951SerArg: 1.951 ± 0.04	3.751SerSer: 3.751 ± 0.065	3.389SerThr: 3.389 ± 0.047	3.82SerVal: 3.82 ± 0.062	0.572SerTrp: 0.572 ± 0.024	2.316SerTyr: 2.316 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	4.604ThrAla: 4.604 ± 0.064	0.366ThrCys: 0.366 ± 0.018	2.664ThrAsp: 2.664 ± 0.048	3.558ThrGlu: 3.558 ± 0.055	2.827ThrPhe: 2.827 ± 0.049	3.885ThrGly: 3.885 ± 0.061	1.128ThrHis: 1.128 ± 0.03	5.279ThrIle: 5.279 ± 0.061	3.557ThrLys: 3.557 ± 0.054	5.824ThrLeu: 5.824 ± 0.063	1.603ThrMet: 1.603 ± 0.036	2.768ThrAsn: 2.768 ± 0.051	2.301ThrPro: 2.301 ± 0.044	1.857ThrGln: 1.857 ± 0.038	1.857ThrArg: 1.857 ± 0.041	3.388ThrSer: 3.388 ± 0.053	3.632ThrThr: 3.632 ± 0.06	4.402ThrVal: 4.402 ± 0.068	0.573ThrTrp: 0.573 ± 0.02	2.046ThrTyr: 2.046 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	5.638ValAla: 5.638 ± 0.073	0.575ValCys: 0.575 ± 0.027	3.638ValAsp: 3.638 ± 0.054	4.923ValGlu: 4.923 ± 0.071	3.026ValPhe: 3.026 ± 0.055	4.575ValGly: 4.575 ± 0.069	1.428ValHis: 1.428 ± 0.03	5.391ValIle: 5.391 ± 0.06	4.427ValLys: 4.427 ± 0.065	6.878ValLeu: 6.878 ± 0.077	1.988ValMet: 1.988 ± 0.038	2.909ValAsn: 2.909 ± 0.053	2.616ValPro: 2.616 ± 0.05	2.858ValGln: 2.858 ± 0.044	2.642ValArg: 2.642 ± 0.047	4.267ValSer: 4.267 ± 0.062	4.37ValThr: 4.37 ± 0.072	5.09ValVal: 5.09 ± 0.08	0.605ValTrp: 0.605 ± 0.02	2.265ValTyr: 2.265 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	0.612TrpAla: 0.612 ± 0.026	0.075TrpCys: 0.075 ± 0.007	0.483TrpAsp: 0.483 ± 0.022	0.544TrpGlu: 0.544 ± 0.023	0.456TrpPhe: 0.456 ± 0.018	0.63TrpGly: 0.63 ± 0.021	0.276TrpHis: 0.276 ± 0.014	0.793TrpIle: 0.793 ± 0.026	0.605TrpLys: 0.605 ± 0.023	1.234TrpLeu: 1.234 ± 0.031	0.337TrpMet: 0.337 ± 0.013	0.48TrpAsn: 0.48 ± 0.019	0.249TrpPro: 0.249 ± 0.013	0.556TrpGln: 0.556 ± 0.021	0.419TrpArg: 0.419 ± 0.019	0.608TrpSer: 0.608 ± 0.025	0.54TrpThr: 0.54 ± 0.021	0.585TrpVal: 0.585 ± 0.023	0.123TrpTrp: 0.123 ± 0.01	0.34TrpTyr: 0.34 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.468TyrAla: 2.468 ± 0.047	0.342TyrCys: 0.342 ± 0.018	2.0TyrAsp: 2.0 ± 0.042	2.616TyrGlu: 2.616 ± 0.048	1.869TyrPhe: 1.869 ± 0.042	2.408TyrGly: 2.408 ± 0.044	0.793TyrHis: 0.793 ± 0.026	2.675TyrIle: 2.675 ± 0.047	2.017TyrLys: 2.017 ± 0.042	3.569TyrLeu: 3.569 ± 0.05	0.948TyrMet: 0.948 ± 0.027	1.513TyrAsn: 1.513 ± 0.04	1.349TyrPro: 1.349 ± 0.038	1.569TyrGln: 1.569 ± 0.036	1.604TyrArg: 1.604 ± 0.04	2.098TyrSer: 2.098 ± 0.043	2.016TyrThr: 2.016 ± 0.044	2.448TyrVal: 2.448 ± 0.046	0.41TyrTrp: 0.41 ± 0.018	1.473TyrTyr: 1.473 ± 0.034	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4209 proteins (1268626 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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