Amino acid dipepetide frequency for Clostridium phage phiCT19406C

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.569AlaAla: 1.569 ± 0.723
0.738AlaCys: 0.738 ± 0.317
3.231AlaAsp: 3.231 ± 0.657
5.261AlaGlu: 5.261 ± 1.022
1.477AlaPhe: 1.477 ± 0.459
3.046AlaGly: 3.046 ± 0.847
0.277AlaHis: 0.277 ± 0.205
3.877AlaIle: 3.877 ± 0.599
6.184AlaLys: 6.184 ± 1.025
4.523AlaLeu: 4.523 ± 0.633
1.754AlaMet: 1.754 ± 0.377
3.231AlaAsn: 3.231 ± 0.643
1.569AlaPro: 1.569 ± 0.36
1.108AlaGln: 1.108 ± 0.409
2.584AlaArg: 2.584 ± 0.483
3.507AlaSer: 3.507 ± 1.066
2.769AlaThr: 2.769 ± 0.715
2.308AlaVal: 2.308 ± 0.458
0.738AlaTrp: 0.738 ± 0.232
1.2AlaTyr: 1.2 ± 0.279
0.0AlaXaa: 0.0 ± 0.0
Cys
0.369CysAla: 0.369 ± 0.173
0.277CysCys: 0.277 ± 0.232
0.462CysAsp: 0.462 ± 0.192
1.292CysGlu: 1.292 ± 0.312
0.369CysPhe: 0.369 ± 0.192
0.738CysGly: 0.738 ± 0.259
0.277CysHis: 0.277 ± 0.15
1.2CysIle: 1.2 ± 0.319
1.108CysLys: 1.108 ± 0.391
0.831CysLeu: 0.831 ± 0.314
0.277CysMet: 0.277 ± 0.161
0.462CysAsn: 0.462 ± 0.205
0.462CysPro: 0.462 ± 0.222
0.462CysGln: 0.462 ± 0.208
0.646CysArg: 0.646 ± 0.24
0.738CysSer: 0.738 ± 0.293
0.369CysThr: 0.369 ± 0.189
0.923CysVal: 0.923 ± 0.292
0.185CysTrp: 0.185 ± 0.134
0.462CysTyr: 0.462 ± 0.344
0.0CysXaa: 0.0 ± 0.0
Asp
1.661AspAla: 1.661 ± 0.403
0.646AspCys: 0.646 ± 0.26
1.754AspAsp: 1.754 ± 0.426
4.338AspGlu: 4.338 ± 0.771
1.754AspPhe: 1.754 ± 0.353
4.061AspGly: 4.061 ± 0.55
0.0AspHis: 0.0 ± 0.0
5.907AspIle: 5.907 ± 0.758
7.107AspLys: 7.107 ± 0.874
6.277AspLeu: 6.277 ± 0.788
1.2AspMet: 1.2 ± 0.267
4.43AspAsn: 4.43 ± 0.902
0.831AspPro: 0.831 ± 0.222
0.646AspGln: 0.646 ± 0.224
2.861AspArg: 2.861 ± 0.648
3.231AspSer: 3.231 ± 0.499
2.861AspThr: 2.861 ± 0.418
2.861AspVal: 2.861 ± 0.729
0.738AspTrp: 0.738 ± 0.268
3.231AspTyr: 3.231 ± 0.497
0.0AspXaa: 0.0 ± 0.0
Glu
3.692GluAla: 3.692 ± 0.464
0.831GluCys: 0.831 ± 0.283
5.907GluAsp: 5.907 ± 0.726
9.507GluGlu: 9.507 ± 0.893
4.246GluPhe: 4.246 ± 0.688
4.615GluGly: 4.615 ± 0.585
0.923GluHis: 0.923 ± 0.29
9.23GluIle: 9.23 ± 1.046
9.876GluLys: 9.876 ± 1.078
10.615GluLeu: 10.615 ± 1.074
3.323GluMet: 3.323 ± 0.56
6.83GluAsn: 6.83 ± 0.747
1.015GluPro: 1.015 ± 0.333
3.784GluGln: 3.784 ± 0.751
2.954GluArg: 2.954 ± 0.799
3.6GluSer: 3.6 ± 0.565
4.892GluThr: 4.892 ± 0.661
4.8GluVal: 4.8 ± 0.718
0.923GluTrp: 0.923 ± 0.273
4.43GluTyr: 4.43 ± 0.807
0.0GluXaa: 0.0 ± 0.0
Phe
1.938PheAla: 1.938 ± 0.368
0.646PheCys: 0.646 ± 0.258
2.031PheAsp: 2.031 ± 0.406
2.031PheGlu: 2.031 ± 0.479
0.738PhePhe: 0.738 ± 0.309
1.754PheGly: 1.754 ± 0.346
0.369PheHis: 0.369 ± 0.169
3.692PheIle: 3.692 ± 0.724
4.43PheLys: 4.43 ± 0.632
2.123PheLeu: 2.123 ± 0.429
0.831PheMet: 0.831 ± 0.263
3.6PheAsn: 3.6 ± 0.649
0.831PhePro: 0.831 ± 0.276
0.646PheGln: 0.646 ± 0.24
1.477PheArg: 1.477 ± 0.396
2.123PheSer: 2.123 ± 0.373
1.2PheThr: 1.2 ± 0.342
1.754PheVal: 1.754 ± 0.371
0.185PheTrp: 0.185 ± 0.135
1.569PheTyr: 1.569 ± 0.412
0.0PheXaa: 0.0 ± 0.0
Gly
3.507GlyAla: 3.507 ± 0.874
0.831GlyCys: 0.831 ± 0.297
3.692GlyAsp: 3.692 ± 0.771
4.615GlyGlu: 4.615 ± 0.587
1.661GlyPhe: 1.661 ± 0.432
2.954GlyGly: 2.954 ± 0.5
0.923GlyHis: 0.923 ± 0.374
5.169GlyIle: 5.169 ± 0.679
5.538GlyLys: 5.538 ± 0.713
4.892GlyLeu: 4.892 ± 0.79
1.477GlyMet: 1.477 ± 0.45
2.954GlyAsn: 2.954 ± 0.549
0.277GlyPro: 0.277 ± 0.14
1.385GlyGln: 1.385 ± 0.308
2.123GlyArg: 2.123 ± 0.388
2.492GlySer: 2.492 ± 0.477
3.323GlyThr: 3.323 ± 0.573
2.584GlyVal: 2.584 ± 0.446
0.646GlyTrp: 0.646 ± 0.218
2.769GlyTyr: 2.769 ± 0.45
0.0GlyXaa: 0.0 ± 0.0
His
0.738HisAla: 0.738 ± 0.262
0.185HisCys: 0.185 ± 0.137
0.738HisAsp: 0.738 ± 0.292
0.554HisGlu: 0.554 ± 0.226
0.277HisPhe: 0.277 ± 0.138
0.462HisGly: 0.462 ± 0.297
0.369HisHis: 0.369 ± 0.199
1.108HisIle: 1.108 ± 0.268
1.477HisLys: 1.477 ± 0.451
0.738HisLeu: 0.738 ± 0.165
0.277HisMet: 0.277 ± 0.157
0.738HisAsn: 0.738 ± 0.245
0.092HisPro: 0.092 ± 0.079
0.369HisGln: 0.369 ± 0.151
0.369HisArg: 0.369 ± 0.172
0.646HisSer: 0.646 ± 0.218
0.646HisThr: 0.646 ± 0.2
0.646HisVal: 0.646 ± 0.197
0.277HisTrp: 0.277 ± 0.192
0.369HisTyr: 0.369 ± 0.167
0.0HisXaa: 0.0 ± 0.0
Ile
4.523IleAla: 4.523 ± 0.613
1.108IleCys: 1.108 ± 0.411
4.892IleAsp: 4.892 ± 0.663
8.492IleGlu: 8.492 ± 1.313
2.677IlePhe: 2.677 ± 0.39
3.323IleGly: 3.323 ± 0.543
0.738IleHis: 0.738 ± 0.283
6.184IleIle: 6.184 ± 1.016
10.43IleLys: 10.43 ± 0.851
7.015IleLeu: 7.015 ± 1.051
1.754IleMet: 1.754 ± 0.347
5.354IleAsn: 5.354 ± 0.598
1.754IlePro: 1.754 ± 0.401
2.308IleGln: 2.308 ± 0.49
3.969IleArg: 3.969 ± 0.828
4.338IleSer: 4.338 ± 0.646
4.8IleThr: 4.8 ± 0.89
4.246IleVal: 4.246 ± 0.579
0.369IleTrp: 0.369 ± 0.203
3.138IleTyr: 3.138 ± 0.585
0.0IleXaa: 0.0 ± 0.0
Lys
6.83LysAla: 6.83 ± 0.875
0.831LysCys: 0.831 ± 0.263
6.461LysAsp: 6.461 ± 0.731
13.291LysGlu: 13.291 ± 1.025
4.061LysPhe: 4.061 ± 0.725
4.8LysGly: 4.8 ± 0.628
1.661LysHis: 1.661 ± 0.399
7.938LysIle: 7.938 ± 0.897
10.522LysLys: 10.522 ± 1.193
9.876LysLeu: 9.876 ± 1.047
4.246LysMet: 4.246 ± 0.811
8.03LysAsn: 8.03 ± 1.009
2.123LysPro: 2.123 ± 0.417
4.246LysGln: 4.246 ± 0.703
5.169LysArg: 5.169 ± 0.886
5.261LysSer: 5.261 ± 0.58
5.538LysThr: 5.538 ± 0.893
7.292LysVal: 7.292 ± 0.858
1.015LysTrp: 1.015 ± 0.219
4.523LysTyr: 4.523 ± 0.734
0.0LysXaa: 0.0 ± 0.0
Leu
4.338LeuAla: 4.338 ± 1.002
0.831LeuCys: 0.831 ± 0.357
5.815LeuAsp: 5.815 ± 0.701
9.876LeuGlu: 9.876 ± 1.101
2.4LeuPhe: 2.4 ± 0.465
5.446LeuGly: 5.446 ± 0.743
1.292LeuHis: 1.292 ± 0.33
4.523LeuIle: 4.523 ± 0.673
9.415LeuLys: 9.415 ± 1.054
5.907LeuLeu: 5.907 ± 0.833
2.215LeuMet: 2.215 ± 0.404
8.215LeuAsn: 8.215 ± 0.852
1.754LeuPro: 1.754 ± 0.414
3.138LeuGln: 3.138 ± 0.568
2.308LeuArg: 2.308 ± 0.519
4.246LeuSer: 4.246 ± 0.663
4.984LeuThr: 4.984 ± 0.818
3.784LeuVal: 3.784 ± 0.631
1.108LeuTrp: 1.108 ± 0.34
3.6LeuTyr: 3.6 ± 0.604
0.0LeuXaa: 0.0 ± 0.0
Met
1.754MetAla: 1.754 ± 0.529
0.185MetCys: 0.185 ± 0.121
1.477MetAsp: 1.477 ± 0.391
2.677MetGlu: 2.677 ± 0.565
1.015MetPhe: 1.015 ± 0.391
1.477MetGly: 1.477 ± 0.504
0.185MetHis: 0.185 ± 0.194
2.308MetIle: 2.308 ± 0.454
3.138MetLys: 3.138 ± 0.647
2.584MetLeu: 2.584 ± 0.384
0.369MetMet: 0.369 ± 0.166
2.308MetAsn: 2.308 ± 0.375
0.646MetPro: 0.646 ± 0.2
0.646MetGln: 0.646 ± 0.222
1.015MetArg: 1.015 ± 0.386
1.938MetSer: 1.938 ± 0.456
1.385MetThr: 1.385 ± 0.345
1.661MetVal: 1.661 ± 0.429
0.369MetTrp: 0.369 ± 0.195
0.923MetTyr: 0.923 ± 0.316
0.0MetXaa: 0.0 ± 0.0
Asn
3.784AsnAla: 3.784 ± 0.618
0.831AsnCys: 0.831 ± 0.246
2.769AsnAsp: 2.769 ± 0.555
5.815AsnGlu: 5.815 ± 0.738
1.846AsnPhe: 1.846 ± 0.427
4.154AsnGly: 4.154 ± 0.557
0.462AsnHis: 0.462 ± 0.157
5.815AsnIle: 5.815 ± 0.91
9.415AsnLys: 9.415 ± 0.891
5.815AsnLeu: 5.815 ± 0.701
2.677AsnMet: 2.677 ± 0.504
6.0AsnAsn: 6.0 ± 0.874
2.4AsnPro: 2.4 ± 0.39
1.477AsnGln: 1.477 ± 0.417
3.046AsnArg: 3.046 ± 0.506
4.707AsnSer: 4.707 ± 0.701
3.784AsnThr: 3.784 ± 0.489
3.323AsnVal: 3.323 ± 0.473
0.831AsnTrp: 0.831 ± 0.245
3.138AsnTyr: 3.138 ± 0.411
0.0AsnXaa: 0.0 ± 0.0
Pro
0.554ProAla: 0.554 ± 0.281
0.369ProCys: 0.369 ± 0.175
1.015ProAsp: 1.015 ± 0.282
1.661ProGlu: 1.661 ± 0.315
1.015ProPhe: 1.015 ± 0.282
0.554ProGly: 0.554 ± 0.209
0.369ProHis: 0.369 ± 0.159
1.754ProIle: 1.754 ± 0.444
1.569ProLys: 1.569 ± 0.478
1.569ProLeu: 1.569 ± 0.328
0.277ProMet: 0.277 ± 0.138
1.569ProAsn: 1.569 ± 0.533
0.554ProPro: 0.554 ± 0.241
0.092ProGln: 0.092 ± 0.107
0.923ProArg: 0.923 ± 0.327
1.569ProSer: 1.569 ± 0.322
1.754ProThr: 1.754 ± 0.524
1.292ProVal: 1.292 ± 0.296
0.277ProTrp: 0.277 ± 0.152
0.831ProTyr: 0.831 ± 0.263
0.0ProXaa: 0.0 ± 0.0
Gln
2.031GlnAla: 2.031 ± 0.572
0.277GlnCys: 0.277 ± 0.164
1.661GlnAsp: 1.661 ± 0.313
3.415GlnGlu: 3.415 ± 0.606
1.015GlnPhe: 1.015 ± 0.276
1.477GlnGly: 1.477 ± 0.338
0.092GlnHis: 0.092 ± 0.087
1.477GlnIle: 1.477 ± 0.307
3.507GlnLys: 3.507 ± 0.652
2.492GlnLeu: 2.492 ± 0.454
1.015GlnMet: 1.015 ± 0.303
1.569GlnAsn: 1.569 ± 0.399
0.831GlnPro: 0.831 ± 0.294
1.385GlnGln: 1.385 ± 0.317
1.2GlnArg: 1.2 ± 0.24
1.938GlnSer: 1.938 ± 0.349
1.846GlnThr: 1.846 ± 0.372
1.661GlnVal: 1.661 ± 0.469
0.092GlnTrp: 0.092 ± 0.089
1.385GlnTyr: 1.385 ± 0.264
0.0GlnXaa: 0.0 ± 0.0
Arg
1.661ArgAla: 1.661 ± 0.462
0.923ArgCys: 0.923 ± 0.267
1.661ArgAsp: 1.661 ± 0.348
4.892ArgGlu: 4.892 ± 0.624
0.923ArgPhe: 0.923 ± 0.305
2.031ArgGly: 2.031 ± 0.457
0.646ArgHis: 0.646 ± 0.232
3.692ArgIle: 3.692 ± 0.669
3.784ArgLys: 3.784 ± 0.692
3.6ArgLeu: 3.6 ± 0.762
0.923ArgMet: 0.923 ± 0.369
2.954ArgAsn: 2.954 ± 0.661
1.015ArgPro: 1.015 ± 0.26
1.938ArgGln: 1.938 ± 0.667
0.738ArgArg: 0.738 ± 0.236
1.569ArgSer: 1.569 ± 0.311
2.031ArgThr: 2.031 ± 0.501
2.584ArgVal: 2.584 ± 0.419
0.369ArgTrp: 0.369 ± 0.161
1.569ArgTyr: 1.569 ± 0.456
0.0ArgXaa: 0.0 ± 0.0
Ser
3.507SerAla: 3.507 ± 0.597
0.831SerCys: 0.831 ± 0.335
2.4SerAsp: 2.4 ± 0.47
4.523SerGlu: 4.523 ± 0.712
2.308SerPhe: 2.308 ± 0.349
2.954SerGly: 2.954 ± 0.572
0.277SerHis: 0.277 ± 0.147
5.538SerIle: 5.538 ± 0.623
5.538SerLys: 5.538 ± 0.87
3.784SerLeu: 3.784 ± 0.583
1.569SerMet: 1.569 ± 0.362
3.138SerAsn: 3.138 ± 0.521
0.462SerPro: 0.462 ± 0.188
1.292SerGln: 1.292 ± 0.337
1.477SerArg: 1.477 ± 0.359
3.784SerSer: 3.784 ± 0.769
2.861SerThr: 2.861 ± 0.499
4.154SerVal: 4.154 ± 0.596
0.369SerTrp: 0.369 ± 0.148
2.954SerTyr: 2.954 ± 0.527
0.0SerXaa: 0.0 ± 0.0
Thr
2.954ThrAla: 2.954 ± 0.952
0.554ThrCys: 0.554 ± 0.196
3.138ThrAsp: 3.138 ± 0.499
4.8ThrGlu: 4.8 ± 0.629
2.123ThrPhe: 2.123 ± 0.471
4.061ThrGly: 4.061 ± 0.921
0.923ThrHis: 0.923 ± 0.345
4.43ThrIle: 4.43 ± 0.59
7.846ThrLys: 7.846 ± 0.903
4.43ThrLeu: 4.43 ± 0.669
0.831ThrMet: 0.831 ± 0.341
3.877ThrAsn: 3.877 ± 0.736
1.015ThrPro: 1.015 ± 0.275
2.215ThrGln: 2.215 ± 0.484
1.754ThrArg: 1.754 ± 0.419
1.477ThrSer: 1.477 ± 0.324
3.6ThrThr: 3.6 ± 0.703
3.415ThrVal: 3.415 ± 0.549
0.646ThrTrp: 0.646 ± 0.32
1.569ThrTyr: 1.569 ± 0.4
0.0ThrXaa: 0.0 ± 0.0
Val
3.231ValAla: 3.231 ± 0.585
0.185ValCys: 0.185 ± 0.149
4.338ValAsp: 4.338 ± 0.598
4.43ValGlu: 4.43 ± 0.62
1.661ValPhe: 1.661 ± 0.382
3.138ValGly: 3.138 ± 0.69
0.738ValHis: 0.738 ± 0.233
3.692ValIle: 3.692 ± 0.489
6.0ValLys: 6.0 ± 0.66
3.784ValLeu: 3.784 ± 0.542
1.477ValMet: 1.477 ± 0.331
3.507ValAsn: 3.507 ± 0.6
1.477ValPro: 1.477 ± 0.346
1.938ValGln: 1.938 ± 0.494
2.308ValArg: 2.308 ± 0.304
3.231ValSer: 3.231 ± 0.592
3.969ValThr: 3.969 ± 0.635
2.861ValVal: 2.861 ± 0.586
0.277ValTrp: 0.277 ± 0.133
2.584ValTyr: 2.584 ± 0.54
0.0ValXaa: 0.0 ± 0.0
Trp
0.554TrpAla: 0.554 ± 0.198
0.092TrpCys: 0.092 ± 0.086
0.831TrpAsp: 0.831 ± 0.291
0.738TrpGlu: 0.738 ± 0.242
0.462TrpPhe: 0.462 ± 0.175
0.369TrpGly: 0.369 ± 0.183
0.185TrpHis: 0.185 ± 0.14
1.015TrpIle: 1.015 ± 0.312
0.923TrpLys: 0.923 ± 0.297
1.108TrpLeu: 1.108 ± 0.3
0.0TrpMet: 0.0 ± 0.0
0.738TrpAsn: 0.738 ± 0.223
0.0TrpPro: 0.0 ± 0.0
0.462TrpGln: 0.462 ± 0.176
0.462TrpArg: 0.462 ± 0.205
0.554TrpSer: 0.554 ± 0.241
0.554TrpThr: 0.554 ± 0.184
0.462TrpVal: 0.462 ± 0.289
0.0TrpTrp: 0.0 ± 0.0
0.277TrpTyr: 0.277 ± 0.183
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.754TyrAla: 1.754 ± 0.409
0.738TyrCys: 0.738 ± 0.299
2.123TyrAsp: 2.123 ± 0.477
3.784TyrGlu: 3.784 ± 0.618
1.938TyrPhe: 1.938 ± 0.399
2.4TyrGly: 2.4 ± 0.442
0.369TyrHis: 0.369 ± 0.184
2.954TyrIle: 2.954 ± 0.423
5.907TyrLys: 5.907 ± 0.984
3.231TyrLeu: 3.231 ± 0.615
1.477TyrMet: 1.477 ± 0.355
2.584TyrAsn: 2.584 ± 0.421
0.462TyrPro: 0.462 ± 0.225
0.831TyrGln: 0.831 ± 0.296
2.123TyrArg: 2.123 ± 0.577
2.769TyrSer: 2.769 ± 0.494
2.492TyrThr: 2.492 ± 0.469
2.215TyrVal: 2.215 ± 0.384
0.369TyrTrp: 0.369 ± 0.163
1.477TyrTyr: 1.477 ± 0.399
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (10835 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski