Methyloligella halotolerans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Methyloligella

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3174 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E2RZ83|A0A1E2RZ83_9RHIZ NAD(P) transhydrogenase subunit beta OS=Methyloligella halotolerans OX=1177755 GN=A7A08_01558 PE=4 SV=1
MM1 pKa = 7.01YY2 pKa = 10.63ADD4 pKa = 3.88LGFRR8 pKa = 11.84NSKK11 pKa = 11.11GEE13 pKa = 3.71VHH15 pKa = 6.64VNVTAADD22 pKa = 3.59TSFGVTASAPVQLLALDD39 pKa = 4.05RR40 pKa = 11.84EE41 pKa = 4.63RR42 pKa = 11.84TFTSPQTTDD51 pKa = 2.9NKK53 pKa = 10.47MIMPSVNASYY63 pKa = 11.05NVNDD67 pKa = 4.59NITLSGVAYY76 pKa = 9.74YY77 pKa = 10.57RR78 pKa = 11.84RR79 pKa = 11.84FEE81 pKa = 4.0QSHH84 pKa = 7.07DD85 pKa = 3.69DD86 pKa = 3.96GNISEE91 pKa = 5.88AEE93 pKa = 4.07PCDD96 pKa = 4.17DD97 pKa = 5.33NPAILCLEE105 pKa = 4.38GEE107 pKa = 4.43EE108 pKa = 4.33ATDD111 pKa = 3.44ISGNLIPTPDD121 pKa = 4.05GEE123 pKa = 4.66LGSIDD128 pKa = 3.72STGQTADD135 pKa = 2.63SWGTAVQMANDD146 pKa = 3.81ADD148 pKa = 4.52LYY150 pKa = 10.19GHH152 pKa = 6.9GNHH155 pKa = 6.99FIIGASYY162 pKa = 11.43DD163 pKa = 3.41HH164 pKa = 6.6GQVTFNAQSEE174 pKa = 4.38LAQFQPRR181 pKa = 11.84FVVTPTGTTFGGPDD195 pKa = 3.18EE196 pKa = 4.51VAPKK200 pKa = 10.77LIDD203 pKa = 3.5TEE205 pKa = 4.1NTYY208 pKa = 11.11VGLYY212 pKa = 10.17ISDD215 pKa = 3.69TYY217 pKa = 11.1EE218 pKa = 3.88VNDD221 pKa = 4.19RR222 pKa = 11.84LAVTLGGRR230 pKa = 11.84YY231 pKa = 9.39NYY233 pKa = 11.13ASIQLTDD240 pKa = 3.85LTGEE244 pKa = 4.25DD245 pKa = 3.65PALNGTNTYY254 pKa = 10.41EE255 pKa = 4.28RR256 pKa = 11.84FNPAAGLTYY265 pKa = 10.61QLTSTISAYY274 pKa = 10.33GGYY277 pKa = 9.49SEE279 pKa = 5.94ANRR282 pKa = 11.84APVPAEE288 pKa = 3.84LACADD293 pKa = 4.12PEE295 pKa = 4.4RR296 pKa = 11.84PCLIEE301 pKa = 4.31SFLVADD307 pKa = 4.52PPLEE311 pKa = 3.9QVVSNTWEE319 pKa = 3.92LGLRR323 pKa = 11.84GEE325 pKa = 4.62DD326 pKa = 3.3TAADD330 pKa = 4.18GSSRR334 pKa = 11.84WTWNAGLFRR343 pKa = 11.84TYY345 pKa = 10.72TDD347 pKa = 3.85DD348 pKa = 6.22DD349 pKa = 4.68IITVVTNSTRR359 pKa = 11.84GYY361 pKa = 8.42FQNGADD367 pKa = 3.82ILRR370 pKa = 11.84QGIEE374 pKa = 3.9AGLTYY379 pKa = 9.02QTQKK383 pKa = 7.94WQAYY387 pKa = 9.2ANYY390 pKa = 9.91AYY392 pKa = 9.75IDD394 pKa = 3.32ATYY397 pKa = 11.08QNDD400 pKa = 4.99LILNAPDD407 pKa = 3.54NPSARR412 pKa = 11.84EE413 pKa = 4.15SICDD417 pKa = 3.22ASDD420 pKa = 3.58FEE422 pKa = 4.5EE423 pKa = 4.71EE424 pKa = 4.18EE425 pKa = 4.46EE426 pKa = 4.12EE427 pKa = 5.34GEE429 pKa = 4.94DD430 pKa = 3.96EE431 pKa = 6.23GEE433 pKa = 4.11LACIAVRR440 pKa = 11.84PGDD443 pKa = 4.13QLPGIPPHH451 pKa = 6.46RR452 pKa = 11.84FKK454 pKa = 11.36AGMDD458 pKa = 3.24YY459 pKa = 10.71WVTDD463 pKa = 2.81KK464 pKa = 11.07WKK466 pKa = 10.53IGGDD470 pKa = 3.97LIGVSSQYY478 pKa = 10.82FFGDD482 pKa = 3.47EE483 pKa = 4.55ANQQPQLDD491 pKa = 4.74GYY493 pKa = 9.83WRR495 pKa = 11.84VDD497 pKa = 3.03LHH499 pKa = 6.23TSYY502 pKa = 11.05QITPRR507 pKa = 11.84IQLFALANNVFDD519 pKa = 4.29KK520 pKa = 10.79EE521 pKa = 4.29YY522 pKa = 10.89GVFGTFFNLAGANEE536 pKa = 4.2GAEE539 pKa = 4.19ADD541 pKa = 3.99PDD543 pKa = 3.68LGEE546 pKa = 5.77DD547 pKa = 4.16FFTNPRR553 pKa = 11.84TITPAPPAVIYY564 pKa = 10.53GGAKK568 pKa = 8.34IALWW572 pKa = 3.66

Molecular weight: 62.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E2RZN3|A0A1E2RZN3_9RHIZ Uncharacterized protein OS=Methyloligella halotolerans OX=1177755 GN=A7A08_01709 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 9.63IKK4 pKa = 10.84NSIRR8 pKa = 11.84SLRR11 pKa = 11.84KK12 pKa = 7.71RR13 pKa = 11.84HH14 pKa = 5.29RR15 pKa = 11.84ANRR18 pKa = 11.84VVRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84GRR25 pKa = 11.84LYY27 pKa = 10.74VINKK31 pKa = 7.74INRR34 pKa = 11.84RR35 pKa = 11.84FKK37 pKa = 10.84ARR39 pKa = 11.84QGG41 pKa = 3.34

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3174

0

3174

933872

29

2626

294.2

32.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.587 ± 0.054

0.88 ± 0.015

6.036 ± 0.037

6.627 ± 0.045

3.75 ± 0.029

8.629 ± 0.049

1.962 ± 0.022

5.044 ± 0.031

3.818 ± 0.032

9.89 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.412 ± 0.023

2.64 ± 0.03

5.31 ± 0.038

3.155 ± 0.025

6.646 ± 0.046

5.631 ± 0.033

5.226 ± 0.035

7.01 ± 0.037

1.334 ± 0.019

2.411 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski