Amino acid dipepetide frequency for Arthrobacter phage BossLady

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.739AlaAla: 18.739 ± 2.082
0.809AlaCys: 0.809 ± 0.262
7.408AlaAsp: 7.408 ± 0.958
7.844AlaGlu: 7.844 ± 1.049
3.05AlaPhe: 3.05 ± 0.534
9.712AlaGly: 9.712 ± 1.246
2.117AlaHis: 2.117 ± 0.382
4.918AlaIle: 4.918 ± 0.493
4.856AlaLys: 4.856 ± 0.589
9.961AlaLeu: 9.961 ± 1.048
2.801AlaMet: 2.801 ± 0.412
3.05AlaAsn: 3.05 ± 0.361
5.79AlaPro: 5.79 ± 0.738
4.918AlaGln: 4.918 ± 0.588
8.155AlaArg: 8.155 ± 0.856
6.039AlaSer: 6.039 ± 0.865
6.661AlaThr: 6.661 ± 1.239
7.72AlaVal: 7.72 ± 0.826
1.432AlaTrp: 1.432 ± 0.235
2.615AlaTyr: 2.615 ± 0.36
0.0AlaXaa: 0.0 ± 0.0
Cys
0.436CysAla: 0.436 ± 0.172
0.062CysCys: 0.062 ± 0.063
0.498CysAsp: 0.498 ± 0.191
0.436CysGlu: 0.436 ± 0.141
0.187CysPhe: 0.187 ± 0.102
0.623CysGly: 0.623 ± 0.227
0.187CysHis: 0.187 ± 0.113
0.187CysIle: 0.187 ± 0.099
0.249CysLys: 0.249 ± 0.129
0.747CysLeu: 0.747 ± 0.214
0.249CysMet: 0.249 ± 0.179
0.125CysAsn: 0.125 ± 0.097
0.623CysPro: 0.623 ± 0.232
0.125CysGln: 0.125 ± 0.084
0.436CysArg: 0.436 ± 0.217
0.685CysSer: 0.685 ± 0.21
0.436CysThr: 0.436 ± 0.143
0.56CysVal: 0.56 ± 0.172
0.187CysTrp: 0.187 ± 0.144
0.125CysTyr: 0.125 ± 0.091
0.0CysXaa: 0.0 ± 0.0
Asp
8.155AspAla: 8.155 ± 0.87
0.685AspCys: 0.685 ± 0.213
5.292AspAsp: 5.292 ± 0.664
5.478AspGlu: 5.478 ± 0.759
1.743AspPhe: 1.743 ± 0.301
5.976AspGly: 5.976 ± 0.647
1.307AspHis: 1.307 ± 0.325
2.864AspIle: 2.864 ± 0.416
4.296AspLys: 4.296 ± 0.556
3.86AspLeu: 3.86 ± 0.389
0.934AspMet: 0.934 ± 0.288
2.179AspAsn: 2.179 ± 0.297
2.988AspPro: 2.988 ± 0.403
1.556AspGln: 1.556 ± 0.381
2.739AspArg: 2.739 ± 0.408
2.49AspSer: 2.49 ± 0.355
3.611AspThr: 3.611 ± 0.399
6.225AspVal: 6.225 ± 0.752
1.432AspTrp: 1.432 ± 0.351
1.37AspTyr: 1.37 ± 0.255
0.0AspXaa: 0.0 ± 0.0
Glu
6.599GluAla: 6.599 ± 0.795
0.436GluCys: 0.436 ± 0.185
3.486GluAsp: 3.486 ± 0.626
3.611GluGlu: 3.611 ± 0.477
1.743GluPhe: 1.743 ± 0.326
3.3GluGly: 3.3 ± 0.448
1.681GluHis: 1.681 ± 0.342
3.673GluIle: 3.673 ± 0.413
2.926GluLys: 2.926 ± 0.45
6.786GluLeu: 6.786 ± 0.856
1.93GluMet: 1.93 ± 0.335
1.93GluAsn: 1.93 ± 0.33
3.549GluPro: 3.549 ± 0.448
3.362GluGln: 3.362 ± 0.541
4.296GluArg: 4.296 ± 0.688
3.798GluSer: 3.798 ± 0.474
3.3GluThr: 3.3 ± 0.51
3.549GluVal: 3.549 ± 0.53
0.623GluTrp: 0.623 ± 0.2
1.93GluTyr: 1.93 ± 0.389
0.0GluXaa: 0.0 ± 0.0
Phe
3.237PheAla: 3.237 ± 0.525
0.249PheCys: 0.249 ± 0.138
2.117PheAsp: 2.117 ± 0.316
2.428PheGlu: 2.428 ± 0.385
0.623PhePhe: 0.623 ± 0.229
2.49PheGly: 2.49 ± 0.41
0.311PheHis: 0.311 ± 0.151
1.307PheIle: 1.307 ± 0.239
1.805PheLys: 1.805 ± 0.336
1.307PheLeu: 1.307 ± 0.304
1.058PheMet: 1.058 ± 0.256
1.121PheAsn: 1.121 ± 0.248
1.37PhePro: 1.37 ± 0.353
0.56PheGln: 0.56 ± 0.194
1.307PheArg: 1.307 ± 0.379
1.992PheSer: 1.992 ± 0.349
2.117PheThr: 2.117 ± 0.36
3.175PheVal: 3.175 ± 0.438
0.623PheTrp: 0.623 ± 0.228
0.56PheTyr: 0.56 ± 0.205
0.0PheXaa: 0.0 ± 0.0
Gly
7.844GlyAla: 7.844 ± 0.888
0.187GlyCys: 0.187 ± 0.122
5.354GlyAsp: 5.354 ± 0.553
4.171GlyGlu: 4.171 ± 0.665
2.117GlyPhe: 2.117 ± 0.345
6.412GlyGly: 6.412 ± 1.392
1.307GlyHis: 1.307 ± 0.268
3.486GlyIle: 3.486 ± 0.587
3.424GlyLys: 3.424 ± 0.467
4.545GlyLeu: 4.545 ± 0.666
1.93GlyMet: 1.93 ± 0.279
2.988GlyAsn: 2.988 ± 0.454
2.926GlyPro: 2.926 ± 0.633
2.117GlyGln: 2.117 ± 0.364
5.292GlyArg: 5.292 ± 0.629
4.607GlySer: 4.607 ± 0.499
5.354GlyThr: 5.354 ± 0.692
6.288GlyVal: 6.288 ± 0.516
1.619GlyTrp: 1.619 ± 0.405
2.179GlyTyr: 2.179 ± 0.425
0.0GlyXaa: 0.0 ± 0.0
His
2.615HisAla: 2.615 ± 0.45
0.062HisCys: 0.062 ± 0.061
1.681HisAsp: 1.681 ± 0.332
1.121HisGlu: 1.121 ± 0.292
0.934HisPhe: 0.934 ± 0.223
0.311HisGly: 0.311 ± 0.142
0.311HisHis: 0.311 ± 0.158
0.996HisIle: 0.996 ± 0.252
0.872HisLys: 0.872 ± 0.284
1.556HisLeu: 1.556 ± 0.289
0.436HisMet: 0.436 ± 0.16
0.623HisAsn: 0.623 ± 0.176
0.809HisPro: 0.809 ± 0.244
0.436HisGln: 0.436 ± 0.148
0.809HisArg: 0.809 ± 0.271
0.311HisSer: 0.311 ± 0.128
1.681HisThr: 1.681 ± 0.408
1.805HisVal: 1.805 ± 0.308
0.498HisTrp: 0.498 ± 0.182
0.498HisTyr: 0.498 ± 0.18
0.0HisXaa: 0.0 ± 0.0
Ile
5.914IleAla: 5.914 ± 0.72
0.249IleCys: 0.249 ± 0.107
4.047IleAsp: 4.047 ± 0.482
5.043IleGlu: 5.043 ± 0.568
1.494IlePhe: 1.494 ± 0.307
4.545IleGly: 4.545 ± 0.615
0.498IleHis: 0.498 ± 0.158
2.864IleIle: 2.864 ± 0.456
2.677IleLys: 2.677 ± 0.357
2.49IleLeu: 2.49 ± 0.47
1.432IleMet: 1.432 ± 0.354
1.992IleAsn: 1.992 ± 0.294
1.619IlePro: 1.619 ± 0.329
1.183IleGln: 1.183 ± 0.257
1.93IleArg: 1.93 ± 0.362
2.552IleSer: 2.552 ± 0.344
3.3IleThr: 3.3 ± 0.487
4.669IleVal: 4.669 ± 0.69
0.498IleTrp: 0.498 ± 0.185
1.245IleTyr: 1.245 ± 0.26
0.0IleXaa: 0.0 ± 0.0
Lys
5.105LysAla: 5.105 ± 0.664
0.436LysCys: 0.436 ± 0.177
1.494LysAsp: 1.494 ± 0.268
1.992LysGlu: 1.992 ± 0.318
1.743LysPhe: 1.743 ± 0.292
3.611LysGly: 3.611 ± 0.418
1.245LysHis: 1.245 ± 0.342
2.677LysIle: 2.677 ± 0.438
1.121LysLys: 1.121 ± 0.287
5.914LysLeu: 5.914 ± 0.624
1.432LysMet: 1.432 ± 0.342
0.934LysAsn: 0.934 ± 0.254
2.801LysPro: 2.801 ± 0.538
2.739LysGln: 2.739 ± 0.405
4.047LysArg: 4.047 ± 0.64
2.303LysSer: 2.303 ± 0.339
3.3LysThr: 3.3 ± 0.424
2.615LysVal: 2.615 ± 0.473
0.747LysTrp: 0.747 ± 0.218
1.121LysTyr: 1.121 ± 0.304
0.0LysXaa: 0.0 ± 0.0
Leu
8.031LeuAla: 8.031 ± 0.657
0.249LeuCys: 0.249 ± 0.123
5.292LeuAsp: 5.292 ± 0.538
3.673LeuGlu: 3.673 ± 0.44
2.054LeuPhe: 2.054 ± 0.294
5.229LeuGly: 5.229 ± 0.912
1.432LeuHis: 1.432 ± 0.296
4.047LeuIle: 4.047 ± 0.602
3.673LeuLys: 3.673 ± 0.46
6.973LeuLeu: 6.973 ± 0.906
2.054LeuMet: 2.054 ± 0.507
2.739LeuAsn: 2.739 ± 0.358
4.109LeuPro: 4.109 ± 0.504
3.486LeuGln: 3.486 ± 0.477
5.665LeuArg: 5.665 ± 0.643
5.167LeuSer: 5.167 ± 0.612
6.537LeuThr: 6.537 ± 0.586
6.101LeuVal: 6.101 ± 0.648
0.685LeuTrp: 0.685 ± 0.293
1.743LeuTyr: 1.743 ± 0.295
0.0LeuXaa: 0.0 ± 0.0
Met
2.801MetAla: 2.801 ± 0.466
0.249MetCys: 0.249 ± 0.136
0.809MetAsp: 0.809 ± 0.229
0.747MetGlu: 0.747 ± 0.299
0.498MetPhe: 0.498 ± 0.147
1.245MetGly: 1.245 ± 0.328
0.374MetHis: 0.374 ± 0.168
0.872MetIle: 0.872 ± 0.246
1.121MetLys: 1.121 ± 0.327
1.619MetLeu: 1.619 ± 0.312
0.56MetMet: 0.56 ± 0.217
0.747MetAsn: 0.747 ± 0.225
1.432MetPro: 1.432 ± 0.379
1.245MetGln: 1.245 ± 0.212
2.241MetArg: 2.241 ± 0.318
2.241MetSer: 2.241 ± 0.383
3.237MetThr: 3.237 ± 0.405
0.934MetVal: 0.934 ± 0.224
0.187MetTrp: 0.187 ± 0.109
0.56MetTyr: 0.56 ± 0.185
0.0MetXaa: 0.0 ± 0.0
Asn
3.922AsnAla: 3.922 ± 0.465
0.436AsnCys: 0.436 ± 0.158
1.743AsnAsp: 1.743 ± 0.356
2.801AsnGlu: 2.801 ± 0.416
0.685AsnPhe: 0.685 ± 0.174
3.05AsnGly: 3.05 ± 0.408
0.56AsnHis: 0.56 ± 0.243
2.241AsnIle: 2.241 ± 0.365
1.868AsnLys: 1.868 ± 0.353
2.366AsnLeu: 2.366 ± 0.348
0.685AsnMet: 0.685 ± 0.203
1.37AsnAsn: 1.37 ± 0.39
2.303AsnPro: 2.303 ± 0.47
1.121AsnGln: 1.121 ± 0.352
1.743AsnArg: 1.743 ± 0.356
1.432AsnSer: 1.432 ± 0.183
1.992AsnThr: 1.992 ± 0.433
2.428AsnVal: 2.428 ± 0.357
1.121AsnTrp: 1.121 ± 0.247
0.747AsnTyr: 0.747 ± 0.219
0.0AsnXaa: 0.0 ± 0.0
Pro
7.595ProAla: 7.595 ± 0.896
0.374ProCys: 0.374 ± 0.139
3.611ProAsp: 3.611 ± 0.678
3.113ProGlu: 3.113 ± 0.429
0.809ProPhe: 0.809 ± 0.209
3.798ProGly: 3.798 ± 0.615
1.183ProHis: 1.183 ± 0.276
3.113ProIle: 3.113 ± 0.614
1.743ProLys: 1.743 ± 0.294
3.735ProLeu: 3.735 ± 0.55
0.56ProMet: 0.56 ± 0.171
1.805ProAsn: 1.805 ± 0.475
2.677ProPro: 2.677 ± 0.722
1.619ProGln: 1.619 ± 0.286
2.366ProArg: 2.366 ± 0.381
2.739ProSer: 2.739 ± 0.42
3.113ProThr: 3.113 ± 0.547
3.113ProVal: 3.113 ± 0.455
0.809ProTrp: 0.809 ± 0.204
1.556ProTyr: 1.556 ± 0.383
0.0ProXaa: 0.0 ± 0.0
Gln
3.984GlnAla: 3.984 ± 0.523
0.56GlnCys: 0.56 ± 0.221
1.494GlnAsp: 1.494 ± 0.239
1.556GlnGlu: 1.556 ± 0.313
1.245GlnPhe: 1.245 ± 0.326
2.677GlnGly: 2.677 ± 0.431
0.996GlnHis: 0.996 ± 0.269
2.054GlnIle: 2.054 ± 0.394
1.805GlnLys: 1.805 ± 0.338
3.86GlnLeu: 3.86 ± 0.609
0.934GlnMet: 0.934 ± 0.302
1.121GlnAsn: 1.121 ± 0.192
1.681GlnPro: 1.681 ± 0.356
2.303GlnGln: 2.303 ± 0.47
3.424GlnArg: 3.424 ± 0.543
2.49GlnSer: 2.49 ± 0.415
2.615GlnThr: 2.615 ± 0.386
2.428GlnVal: 2.428 ± 0.452
0.311GlnTrp: 0.311 ± 0.115
0.623GlnTyr: 0.623 ± 0.181
0.0GlnXaa: 0.0 ± 0.0
Arg
6.848ArgAla: 6.848 ± 0.549
0.685ArgCys: 0.685 ± 0.227
4.607ArgAsp: 4.607 ± 0.569
4.918ArgGlu: 4.918 ± 0.744
2.179ArgPhe: 2.179 ± 0.39
3.735ArgGly: 3.735 ± 0.489
1.058ArgHis: 1.058 ± 0.309
2.615ArgIle: 2.615 ± 0.44
3.673ArgLys: 3.673 ± 0.529
5.167ArgLeu: 5.167 ± 0.68
1.307ArgMet: 1.307 ± 0.311
2.49ArgAsn: 2.49 ± 0.436
2.864ArgPro: 2.864 ± 0.496
2.428ArgGln: 2.428 ± 0.393
3.113ArgArg: 3.113 ± 0.614
3.611ArgSer: 3.611 ± 0.405
3.735ArgThr: 3.735 ± 0.532
4.545ArgVal: 4.545 ± 0.769
1.432ArgTrp: 1.432 ± 0.314
1.556ArgTyr: 1.556 ± 0.354
0.0ArgXaa: 0.0 ± 0.0
Ser
6.288SerAla: 6.288 ± 0.91
0.374SerCys: 0.374 ± 0.157
3.611SerAsp: 3.611 ± 0.432
2.864SerGlu: 2.864 ± 0.356
2.179SerPhe: 2.179 ± 0.37
4.669SerGly: 4.669 ± 0.71
0.996SerHis: 0.996 ± 0.28
3.735SerIle: 3.735 ± 0.601
2.988SerLys: 2.988 ± 0.424
3.86SerLeu: 3.86 ± 0.545
1.743SerMet: 1.743 ± 0.288
2.117SerAsn: 2.117 ± 0.431
1.992SerPro: 1.992 ± 0.353
1.805SerGln: 1.805 ± 0.265
2.677SerArg: 2.677 ± 0.421
3.486SerSer: 3.486 ± 0.707
4.42SerThr: 4.42 ± 0.539
4.856SerVal: 4.856 ± 0.579
0.747SerTrp: 0.747 ± 0.232
0.996SerTyr: 0.996 ± 0.209
0.0SerXaa: 0.0 ± 0.0
Thr
8.653ThrAla: 8.653 ± 1.319
0.498ThrCys: 0.498 ± 0.218
3.549ThrAsp: 3.549 ± 0.521
3.735ThrGlu: 3.735 ± 0.509
2.926ThrPhe: 2.926 ± 0.442
4.794ThrGly: 4.794 ± 0.53
1.619ThrHis: 1.619 ± 0.382
3.237ThrIle: 3.237 ± 0.478
2.366ThrLys: 2.366 ± 0.396
5.416ThrLeu: 5.416 ± 0.705
1.307ThrMet: 1.307 ± 0.258
2.054ThrAsn: 2.054 ± 0.402
4.42ThrPro: 4.42 ± 0.584
2.366ThrGln: 2.366 ± 0.399
4.42ThrArg: 4.42 ± 0.47
3.237ThrSer: 3.237 ± 0.6
5.043ThrThr: 5.043 ± 0.692
5.665ThrVal: 5.665 ± 0.69
0.623ThrTrp: 0.623 ± 0.256
1.556ThrTyr: 1.556 ± 0.386
0.0ThrXaa: 0.0 ± 0.0
Val
7.782ValAla: 7.782 ± 0.731
0.249ValCys: 0.249 ± 0.117
6.288ValAsp: 6.288 ± 0.571
4.918ValGlu: 4.918 ± 0.602
2.179ValPhe: 2.179 ± 0.351
4.669ValGly: 4.669 ± 0.683
0.623ValHis: 0.623 ± 0.197
4.171ValIle: 4.171 ± 0.583
3.922ValLys: 3.922 ± 0.45
5.976ValLeu: 5.976 ± 0.671
1.494ValMet: 1.494 ± 0.279
3.362ValAsn: 3.362 ± 0.427
3.798ValPro: 3.798 ± 0.494
3.424ValGln: 3.424 ± 0.54
5.105ValArg: 5.105 ± 0.704
4.98ValSer: 4.98 ± 0.608
5.478ValThr: 5.478 ± 0.666
6.412ValVal: 6.412 ± 0.728
0.934ValTrp: 0.934 ± 0.25
1.432ValTyr: 1.432 ± 0.271
0.0ValXaa: 0.0 ± 0.0
Trp
1.805TrpAla: 1.805 ± 0.378
0.125TrpCys: 0.125 ± 0.087
0.996TrpAsp: 0.996 ± 0.273
0.934TrpGlu: 0.934 ± 0.202
0.56TrpPhe: 0.56 ± 0.204
0.872TrpGly: 0.872 ± 0.231
0.374TrpHis: 0.374 ± 0.15
0.685TrpIle: 0.685 ± 0.262
0.747TrpLys: 0.747 ± 0.204
1.494TrpLeu: 1.494 ± 0.321
0.311TrpMet: 0.311 ± 0.187
0.685TrpAsn: 0.685 ± 0.186
0.685TrpPro: 0.685 ± 0.225
0.436TrpGln: 0.436 ± 0.165
1.245TrpArg: 1.245 ± 0.31
0.685TrpSer: 0.685 ± 0.203
0.685TrpThr: 0.685 ± 0.197
1.183TrpVal: 1.183 ± 0.239
0.249TrpTrp: 0.249 ± 0.118
0.311TrpTyr: 0.311 ± 0.132
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.241TyrAla: 2.241 ± 0.311
0.187TyrCys: 0.187 ± 0.097
1.805TyrAsp: 1.805 ± 0.331
1.121TyrGlu: 1.121 ± 0.317
0.809TyrPhe: 0.809 ± 0.199
2.428TyrGly: 2.428 ± 0.417
0.187TyrHis: 0.187 ± 0.101
0.498TyrIle: 0.498 ± 0.193
1.307TyrLys: 1.307 ± 0.351
1.307TyrLeu: 1.307 ± 0.295
0.498TyrMet: 0.498 ± 0.177
1.183TyrAsn: 1.183 ± 0.211
0.996TyrPro: 0.996 ± 0.254
0.934TyrGln: 0.934 ± 0.232
1.556TyrArg: 1.556 ± 0.42
1.556TyrSer: 1.556 ± 0.355
0.872TyrThr: 0.872 ± 0.203
2.926TyrVal: 2.926 ± 0.461
0.311TyrTrp: 0.311 ± 0.138
0.374TyrTyr: 0.374 ± 0.169
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (16064 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski