Amino acid dipepetide frequency for Pseudomonas phage LKA1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.359AlaAla: 14.359 ± 1.301
0.993AlaCys: 0.993 ± 0.328
5.27AlaAsp: 5.27 ± 0.566
9.471AlaGlu: 9.471 ± 0.98
3.361AlaPhe: 3.361 ± 0.38
8.783AlaGly: 8.783 ± 1.14
1.909AlaHis: 1.909 ± 0.346
4.277AlaIle: 4.277 ± 0.608
5.423AlaLys: 5.423 ± 0.62
12.526AlaLeu: 12.526 ± 1.448
2.673AlaMet: 2.673 ± 0.43
3.666AlaAsn: 3.666 ± 0.439
4.506AlaPro: 4.506 ± 0.683
6.568AlaGln: 6.568 ± 0.641
5.652AlaArg: 5.652 ± 0.691
6.339AlaSer: 6.339 ± 0.956
6.187AlaThr: 6.187 ± 0.927
7.867AlaVal: 7.867 ± 0.592
1.986AlaTrp: 1.986 ± 0.301
3.666AlaTyr: 3.666 ± 0.61
0.0AlaXaa: 0.0 ± 0.0
Cys
1.222CysAla: 1.222 ± 0.319
0.153CysCys: 0.153 ± 0.105
0.535CysAsp: 0.535 ± 0.185
0.611CysGlu: 0.611 ± 0.24
0.306CysPhe: 0.306 ± 0.162
1.069CysGly: 1.069 ± 0.314
0.382CysHis: 0.382 ± 0.205
0.535CysIle: 0.535 ± 0.228
0.764CysLys: 0.764 ± 0.298
1.298CysLeu: 1.298 ± 0.325
0.382CysMet: 0.382 ± 0.158
0.458CysAsn: 0.458 ± 0.181
0.458CysPro: 0.458 ± 0.182
0.611CysGln: 0.611 ± 0.192
0.764CysArg: 0.764 ± 0.254
0.687CysSer: 0.687 ± 0.224
0.764CysThr: 0.764 ± 0.353
0.535CysVal: 0.535 ± 0.199
0.076CysTrp: 0.076 ± 0.075
0.535CysTyr: 0.535 ± 0.197
0.0CysXaa: 0.0 ± 0.0
Asp
7.256AspAla: 7.256 ± 0.851
0.535AspCys: 0.535 ± 0.2
3.895AspAsp: 3.895 ± 0.651
4.506AspGlu: 4.506 ± 0.57
2.368AspPhe: 2.368 ± 0.414
4.506AspGly: 4.506 ± 0.677
1.069AspHis: 1.069 ± 0.305
2.673AspIle: 2.673 ± 0.339
2.979AspLys: 2.979 ± 0.461
4.583AspLeu: 4.583 ± 0.574
1.528AspMet: 1.528 ± 0.357
1.069AspAsn: 1.069 ± 0.286
3.055AspPro: 3.055 ± 0.346
1.68AspGln: 1.68 ± 0.332
3.131AspArg: 3.131 ± 0.514
2.139AspSer: 2.139 ± 0.536
3.208AspThr: 3.208 ± 0.497
3.972AspVal: 3.972 ± 0.477
1.146AspTrp: 1.146 ± 0.275
1.909AspTyr: 1.909 ± 0.314
0.0AspXaa: 0.0 ± 0.0
Glu
9.318GluAla: 9.318 ± 0.895
0.611GluCys: 0.611 ± 0.222
3.437GluAsp: 3.437 ± 0.468
3.972GluGlu: 3.972 ± 0.453
2.826GluPhe: 2.826 ± 0.441
3.819GluGly: 3.819 ± 0.714
1.68GluHis: 1.68 ± 0.445
1.146GluIle: 1.146 ± 0.322
2.368GluLys: 2.368 ± 0.384
7.103GluLeu: 7.103 ± 0.821
1.833GluMet: 1.833 ± 0.295
1.909GluAsn: 1.909 ± 0.397
1.68GluPro: 1.68 ± 0.496
2.902GluGln: 2.902 ± 0.416
5.346GluArg: 5.346 ± 0.666
2.673GluSer: 2.673 ± 0.44
3.208GluThr: 3.208 ± 0.475
5.728GluVal: 5.728 ± 0.722
0.611GluTrp: 0.611 ± 0.204
3.055GluTyr: 3.055 ± 0.464
0.0GluXaa: 0.0 ± 0.0
Phe
3.972PheAla: 3.972 ± 0.531
0.306PheCys: 0.306 ± 0.126
1.986PheAsp: 1.986 ± 0.32
1.757PheGlu: 1.757 ± 0.53
0.458PhePhe: 0.458 ± 0.153
2.444PheGly: 2.444 ± 0.407
0.458PheHis: 0.458 ± 0.16
1.451PheIle: 1.451 ± 0.279
0.84PheLys: 0.84 ± 0.277
2.826PheLeu: 2.826 ± 0.504
0.764PheMet: 0.764 ± 0.213
0.917PheAsn: 0.917 ± 0.24
1.833PhePro: 1.833 ± 0.386
1.451PheGln: 1.451 ± 0.353
2.368PheArg: 2.368 ± 0.447
1.757PheSer: 1.757 ± 0.339
1.986PheThr: 1.986 ± 0.318
1.604PheVal: 1.604 ± 0.333
0.306PheTrp: 0.306 ± 0.162
0.917PheTyr: 0.917 ± 0.316
0.0PheXaa: 0.0 ± 0.0
Gly
8.096GlyAla: 8.096 ± 0.755
0.84GlyCys: 0.84 ± 0.288
4.583GlyAsp: 4.583 ± 0.699
4.201GlyGlu: 4.201 ± 0.647
2.368GlyPhe: 2.368 ± 0.496
7.027GlyGly: 7.027 ± 0.943
1.451GlyHis: 1.451 ± 0.304
3.742GlyIle: 3.742 ± 0.514
4.277GlyLys: 4.277 ± 0.561
7.867GlyLeu: 7.867 ± 1.111
1.298GlyMet: 1.298 ± 0.234
2.444GlyAsn: 2.444 ± 0.44
2.52GlyPro: 2.52 ± 0.449
4.048GlyGln: 4.048 ± 0.463
6.263GlyArg: 6.263 ± 0.685
3.361GlySer: 3.361 ± 0.603
5.423GlyThr: 5.423 ± 0.655
6.034GlyVal: 6.034 ± 0.568
1.68GlyTrp: 1.68 ± 0.295
2.444GlyTyr: 2.444 ± 0.424
0.0GlyXaa: 0.0 ± 0.0
His
2.062HisAla: 2.062 ± 0.567
0.306HisCys: 0.306 ± 0.175
1.68HisAsp: 1.68 ± 0.382
0.993HisGlu: 0.993 ± 0.277
0.993HisPhe: 0.993 ± 0.275
1.833HisGly: 1.833 ± 0.338
0.153HisHis: 0.153 ± 0.088
1.146HisIle: 1.146 ± 0.256
1.069HisLys: 1.069 ± 0.333
2.291HisLeu: 2.291 ± 0.487
0.535HisMet: 0.535 ± 0.209
0.764HisAsn: 0.764 ± 0.204
0.993HisPro: 0.993 ± 0.323
0.764HisGln: 0.764 ± 0.23
2.062HisArg: 2.062 ± 0.498
0.84HisSer: 0.84 ± 0.281
0.458HisThr: 0.458 ± 0.204
1.604HisVal: 1.604 ± 0.333
0.229HisTrp: 0.229 ± 0.127
0.458HisTyr: 0.458 ± 0.191
0.0HisXaa: 0.0 ± 0.0
Ile
4.812IleAla: 4.812 ± 0.523
0.306IleCys: 0.306 ± 0.155
3.666IleAsp: 3.666 ± 0.528
2.52IleGlu: 2.52 ± 0.455
0.993IlePhe: 0.993 ± 0.259
2.902IleGly: 2.902 ± 0.422
0.993IleHis: 0.993 ± 0.345
1.146IleIle: 1.146 ± 0.292
1.451IleLys: 1.451 ± 0.362
3.361IleLeu: 3.361 ± 0.645
0.458IleMet: 0.458 ± 0.174
1.298IleAsn: 1.298 ± 0.348
1.833IlePro: 1.833 ± 0.425
2.368IleGln: 2.368 ± 0.396
2.826IleArg: 2.826 ± 0.507
2.444IleSer: 2.444 ± 0.457
3.208IleThr: 3.208 ± 0.532
2.139IleVal: 2.139 ± 0.349
0.076IleTrp: 0.076 ± 0.066
1.375IleTyr: 1.375 ± 0.404
0.0IleXaa: 0.0 ± 0.0
Lys
5.499LysAla: 5.499 ± 0.798
0.687LysCys: 0.687 ± 0.289
2.826LysAsp: 2.826 ± 0.387
2.826LysGlu: 2.826 ± 0.582
1.451LysPhe: 1.451 ± 0.287
2.826LysGly: 2.826 ± 0.505
0.917LysHis: 0.917 ± 0.24
1.833LysIle: 1.833 ± 0.393
0.993LysLys: 0.993 ± 0.305
4.277LysLeu: 4.277 ± 0.588
0.993LysMet: 0.993 ± 0.245
1.528LysAsn: 1.528 ± 0.31
1.528LysPro: 1.528 ± 0.396
2.597LysGln: 2.597 ± 0.574
2.673LysArg: 2.673 ± 0.489
1.528LysSer: 1.528 ± 0.419
2.062LysThr: 2.062 ± 0.316
2.597LysVal: 2.597 ± 0.409
0.993LysTrp: 0.993 ± 0.279
0.993LysTyr: 0.993 ± 0.265
0.0LysXaa: 0.0 ± 0.0
Leu
10.234LeuAla: 10.234 ± 0.971
1.451LeuCys: 1.451 ± 0.349
6.339LeuAsp: 6.339 ± 0.746
6.416LeuGlu: 6.416 ± 0.575
2.215LeuPhe: 2.215 ± 0.439
8.172LeuGly: 8.172 ± 0.653
1.528LeuHis: 1.528 ± 0.336
3.131LeuIle: 3.131 ± 0.64
2.75LeuLys: 2.75 ± 0.504
8.02LeuLeu: 8.02 ± 0.614
2.139LeuMet: 2.139 ± 0.368
3.055LeuAsn: 3.055 ± 0.361
3.895LeuPro: 3.895 ± 0.505
3.895LeuGln: 3.895 ± 0.588
7.867LeuArg: 7.867 ± 1.012
5.346LeuSer: 5.346 ± 0.694
5.423LeuThr: 5.423 ± 0.919
6.339LeuVal: 6.339 ± 0.803
1.528LeuTrp: 1.528 ± 0.29
3.59LeuTyr: 3.59 ± 0.429
0.0LeuXaa: 0.0 ± 0.0
Met
2.444MetAla: 2.444 ± 0.511
0.229MetCys: 0.229 ± 0.129
0.84MetAsp: 0.84 ± 0.205
0.611MetGlu: 0.611 ± 0.189
0.382MetPhe: 0.382 ± 0.156
1.604MetGly: 1.604 ± 0.377
0.993MetHis: 0.993 ± 0.267
0.611MetIle: 0.611 ± 0.217
0.917MetLys: 0.917 ± 0.243
2.444MetLeu: 2.444 ± 0.332
0.535MetMet: 0.535 ± 0.273
0.84MetAsn: 0.84 ± 0.273
1.146MetPro: 1.146 ± 0.24
1.604MetGln: 1.604 ± 0.385
1.986MetArg: 1.986 ± 0.339
1.757MetSer: 1.757 ± 0.344
1.298MetThr: 1.298 ± 0.367
0.764MetVal: 0.764 ± 0.249
0.153MetTrp: 0.153 ± 0.108
0.993MetTyr: 0.993 ± 0.279
0.0MetXaa: 0.0 ± 0.0
Asn
3.972AsnAla: 3.972 ± 0.642
0.687AsnCys: 0.687 ± 0.31
1.069AsnAsp: 1.069 ± 0.322
1.833AsnGlu: 1.833 ± 0.384
0.993AsnPhe: 0.993 ± 0.237
2.139AsnGly: 2.139 ± 0.392
0.229AsnHis: 0.229 ± 0.128
1.833AsnIle: 1.833 ± 0.416
1.604AsnLys: 1.604 ± 0.366
3.895AsnLeu: 3.895 ± 0.435
0.535AsnMet: 0.535 ± 0.208
0.917AsnAsn: 0.917 ± 0.277
2.291AsnPro: 2.291 ± 0.45
1.298AsnGln: 1.298 ± 0.29
1.986AsnArg: 1.986 ± 0.477
1.451AsnSer: 1.451 ± 0.241
1.222AsnThr: 1.222 ± 0.311
2.139AsnVal: 2.139 ± 0.443
0.687AsnTrp: 0.687 ± 0.193
0.535AsnTyr: 0.535 ± 0.165
0.0AsnXaa: 0.0 ± 0.0
Pro
5.575ProAla: 5.575 ± 0.996
0.611ProCys: 0.611 ± 0.249
2.52ProAsp: 2.52 ± 0.406
4.124ProGlu: 4.124 ± 0.569
1.298ProPhe: 1.298 ± 0.304
4.124ProGly: 4.124 ± 0.658
0.611ProHis: 0.611 ± 0.288
1.757ProIle: 1.757 ± 0.416
2.139ProLys: 2.139 ± 0.332
2.826ProLeu: 2.826 ± 0.401
0.84ProMet: 0.84 ± 0.246
1.222ProAsn: 1.222 ± 0.24
1.68ProPro: 1.68 ± 0.368
2.52ProGln: 2.52 ± 0.528
2.52ProArg: 2.52 ± 0.401
2.597ProSer: 2.597 ± 0.371
2.368ProThr: 2.368 ± 0.483
3.819ProVal: 3.819 ± 0.563
0.611ProTrp: 0.611 ± 0.161
0.917ProTyr: 0.917 ± 0.215
0.0ProXaa: 0.0 ± 0.0
Gln
6.263GlnAla: 6.263 ± 1.031
0.687GlnCys: 0.687 ± 0.285
1.833GlnAsp: 1.833 ± 0.367
3.437GlnGlu: 3.437 ± 0.496
1.451GlnPhe: 1.451 ± 0.254
4.659GlnGly: 4.659 ± 0.571
1.222GlnHis: 1.222 ± 0.309
2.215GlnIle: 2.215 ± 0.475
1.604GlnLys: 1.604 ± 0.352
4.124GlnLeu: 4.124 ± 0.483
1.833GlnMet: 1.833 ± 0.318
0.917GlnAsn: 0.917 ± 0.273
1.833GlnPro: 1.833 ± 0.416
2.444GlnGln: 2.444 ± 0.548
3.055GlnArg: 3.055 ± 0.512
1.909GlnSer: 1.909 ± 0.377
1.909GlnThr: 1.909 ± 0.515
3.513GlnVal: 3.513 ± 0.466
0.917GlnTrp: 0.917 ± 0.249
1.757GlnTyr: 1.757 ± 0.285
0.0GlnXaa: 0.0 ± 0.0
Arg
6.721ArgAla: 6.721 ± 0.922
0.993ArgCys: 0.993 ± 0.28
4.048ArgAsp: 4.048 ± 0.529
4.583ArgGlu: 4.583 ± 0.643
2.291ArgPhe: 2.291 ± 0.365
5.041ArgGly: 5.041 ± 0.554
1.833ArgHis: 1.833 ± 0.422
3.055ArgIle: 3.055 ± 0.462
3.361ArgLys: 3.361 ± 0.578
5.728ArgLeu: 5.728 ± 0.665
1.528ArgMet: 1.528 ± 0.439
2.139ArgAsn: 2.139 ± 0.481
2.673ArgPro: 2.673 ± 0.4
2.368ArgGln: 2.368 ± 0.427
4.583ArgArg: 4.583 ± 0.806
3.361ArgSer: 3.361 ± 0.466
4.659ArgThr: 4.659 ± 0.505
5.957ArgVal: 5.957 ± 0.707
1.222ArgTrp: 1.222 ± 0.361
2.368ArgTyr: 2.368 ± 0.39
0.0ArgXaa: 0.0 ± 0.0
Ser
6.492SerAla: 6.492 ± 0.616
0.84SerCys: 0.84 ± 0.225
2.75SerAsp: 2.75 ± 0.401
2.673SerGlu: 2.673 ± 0.391
1.604SerPhe: 1.604 ± 0.371
5.041SerGly: 5.041 ± 0.755
1.069SerHis: 1.069 ± 0.257
3.284SerIle: 3.284 ± 0.441
2.062SerLys: 2.062 ± 0.346
4.048SerLeu: 4.048 ± 0.639
0.917SerMet: 0.917 ± 0.246
2.062SerAsn: 2.062 ± 0.409
2.139SerPro: 2.139 ± 0.35
2.368SerGln: 2.368 ± 0.458
2.52SerArg: 2.52 ± 0.439
2.75SerSer: 2.75 ± 0.452
3.972SerThr: 3.972 ± 0.616
3.819SerVal: 3.819 ± 0.447
0.458SerTrp: 0.458 ± 0.174
1.604SerTyr: 1.604 ± 0.518
0.0SerXaa: 0.0 ± 0.0
Thr
6.263ThrAla: 6.263 ± 0.802
0.458ThrCys: 0.458 ± 0.242
2.902ThrAsp: 2.902 ± 0.476
2.444ThrGlu: 2.444 ± 0.398
1.986ThrPhe: 1.986 ± 0.365
5.194ThrGly: 5.194 ± 0.633
1.146ThrHis: 1.146 ± 0.214
2.444ThrIle: 2.444 ± 0.456
2.215ThrLys: 2.215 ± 0.484
5.117ThrLeu: 5.117 ± 0.658
1.222ThrMet: 1.222 ± 0.326
1.909ThrAsn: 1.909 ± 0.479
3.742ThrPro: 3.742 ± 0.536
2.597ThrGln: 2.597 ± 0.41
3.208ThrArg: 3.208 ± 0.501
4.353ThrSer: 4.353 ± 0.737
2.826ThrThr: 2.826 ± 0.535
4.201ThrVal: 4.201 ± 0.745
0.917ThrTrp: 0.917 ± 0.256
1.528ThrTyr: 1.528 ± 0.319
0.0ThrXaa: 0.0 ± 0.0
Val
7.485ValAla: 7.485 ± 0.576
0.535ValCys: 0.535 ± 0.169
4.659ValAsp: 4.659 ± 0.486
4.353ValGlu: 4.353 ± 0.538
1.604ValPhe: 1.604 ± 0.32
5.575ValGly: 5.575 ± 0.937
1.909ValHis: 1.909 ± 0.37
1.909ValIle: 1.909 ± 0.336
2.673ValLys: 2.673 ± 0.495
6.645ValLeu: 6.645 ± 0.669
1.528ValMet: 1.528 ± 0.314
2.139ValAsn: 2.139 ± 0.278
4.201ValPro: 4.201 ± 0.776
3.208ValGln: 3.208 ± 0.489
5.575ValArg: 5.575 ± 0.63
3.895ValSer: 3.895 ± 0.523
3.59ValThr: 3.59 ± 0.893
5.728ValVal: 5.728 ± 0.573
1.604ValTrp: 1.604 ± 0.353
2.062ValTyr: 2.062 ± 0.451
0.0ValXaa: 0.0 ± 0.0
Trp
1.68TrpAla: 1.68 ± 0.55
0.306TrpCys: 0.306 ± 0.158
0.993TrpAsp: 0.993 ± 0.242
1.146TrpGlu: 1.146 ± 0.37
0.687TrpPhe: 0.687 ± 0.235
0.917TrpGly: 0.917 ± 0.266
0.382TrpHis: 0.382 ± 0.171
0.764TrpIle: 0.764 ± 0.205
0.611TrpLys: 0.611 ± 0.204
1.451TrpLeu: 1.451 ± 0.387
0.229TrpMet: 0.229 ± 0.129
0.84TrpAsn: 0.84 ± 0.247
0.535TrpPro: 0.535 ± 0.194
1.146TrpGln: 1.146 ± 0.285
0.993TrpArg: 0.993 ± 0.283
1.298TrpSer: 1.298 ± 0.341
0.917TrpThr: 0.917 ± 0.214
0.382TrpVal: 0.382 ± 0.166
0.458TrpTrp: 0.458 ± 0.209
0.764TrpTyr: 0.764 ± 0.318
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.757TyrAla: 1.757 ± 0.323
0.611TyrCys: 0.611 ± 0.197
1.375TyrAsp: 1.375 ± 0.252
2.75TyrGlu: 2.75 ± 0.398
0.764TyrPhe: 0.764 ± 0.239
2.291TyrGly: 2.291 ± 0.401
1.298TyrHis: 1.298 ± 0.343
1.222TyrIle: 1.222 ± 0.271
1.528TyrLys: 1.528 ± 0.398
2.902TyrLeu: 2.902 ± 0.427
0.306TyrMet: 0.306 ± 0.13
1.222TyrAsn: 1.222 ± 0.211
2.139TyrPro: 2.139 ± 0.345
1.146TyrGln: 1.146 ± 0.271
3.131TyrArg: 3.131 ± 0.434
1.986TyrSer: 1.986 ± 0.372
1.986TyrThr: 1.986 ± 0.358
2.139TyrVal: 2.139 ± 0.435
0.917TyrTrp: 0.917 ± 0.26
0.993TyrTyr: 0.993 ± 0.322
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (13094 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski