Amino acid dipepetide frequency for Lachnospiraceae bacterium M18-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.002AlaAla: 8.002 ± 0.106	1.151AlaCys: 1.151 ± 0.028	4.672AlaAsp: 4.672 ± 0.059	6.404AlaGlu: 6.404 ± 0.079	3.065AlaPhe: 3.065 ± 0.054	6.564AlaGly: 6.564 ± 0.066	1.077AlaHis: 1.077 ± 0.027	4.202AlaIle: 4.202 ± 0.059	4.388AlaLys: 4.388 ± 0.054	6.504AlaLeu: 6.504 ± 0.07	2.351AlaMet: 2.351 ± 0.04	2.238AlaAsn: 2.238 ± 0.043	2.01AlaPro: 2.01 ± 0.033	2.309AlaGln: 2.309 ± 0.041	3.198AlaArg: 3.198 ± 0.047	3.858AlaSer: 3.858 ± 0.062	2.493AlaThr: 2.493 ± 0.051	6.67AlaVal: 6.67 ± 0.068	0.702AlaTrp: 0.702 ± 0.02	2.774AlaTyr: 2.774 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	1.059CysAla: 1.059 ± 0.024	0.311CysCys: 0.311 ± 0.017	0.823CysAsp: 0.823 ± 0.023	0.982CysGlu: 0.982 ± 0.025	0.716CysPhe: 0.716 ± 0.021	1.594CysGly: 1.594 ± 0.039	0.286CysHis: 0.286 ± 0.013	1.136CysIle: 1.136 ± 0.03	0.754CysLys: 0.754 ± 0.024	1.289CysLeu: 1.289 ± 0.029	0.531CysMet: 0.531 ± 0.019	0.534CysAsn: 0.534 ± 0.018	0.679CysPro: 0.679 ± 0.022	0.433CysGln: 0.433 ± 0.018	1.007CysArg: 1.007 ± 0.028	0.986CysSer: 0.986 ± 0.021	0.795CysThr: 0.795 ± 0.024	0.95CysVal: 0.95 ± 0.028	0.137CysTrp: 0.137 ± 0.009	0.624CysTyr: 0.624 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	4.101AspAla: 4.101 ± 0.066	0.846AspCys: 0.846 ± 0.025	2.602AspAsp: 2.602 ± 0.052	4.348AspGlu: 4.348 ± 0.059	2.715AspPhe: 2.715 ± 0.044	4.378AspGly: 4.378 ± 0.057	0.916AspHis: 0.916 ± 0.023	4.342AspIle: 4.342 ± 0.056	3.137AspLys: 3.137 ± 0.046	4.615AspLeu: 4.615 ± 0.061	1.832AspMet: 1.832 ± 0.034	1.882AspAsn: 1.882 ± 0.034	1.868AspPro: 1.868 ± 0.036	1.583AspGln: 1.583 ± 0.032	2.8AspArg: 2.8 ± 0.048	3.27AspSer: 3.27 ± 0.05	3.087AspThr: 3.087 ± 0.043	3.511AspVal: 3.511 ± 0.052	0.681AspTrp: 0.681 ± 0.023	2.754AspTyr: 2.754 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	5.849GluAla: 5.849 ± 0.079	0.919GluCys: 0.919 ± 0.026	4.473GluAsp: 4.473 ± 0.065	8.107GluGlu: 8.107 ± 0.096	2.651GluPhe: 2.651 ± 0.039	4.659GluGly: 4.659 ± 0.056	1.524GluHis: 1.524 ± 0.034	5.858GluIle: 5.858 ± 0.06	7.092GluLys: 7.092 ± 0.084	7.115GluLeu: 7.115 ± 0.082	2.614GluMet: 2.614 ± 0.038	4.139GluAsn: 4.139 ± 0.056	2.076GluPro: 2.076 ± 0.043	3.427GluGln: 3.427 ± 0.052	4.026GluArg: 4.026 ± 0.058	3.648GluSer: 3.648 ± 0.049	3.937GluThr: 3.937 ± 0.048	4.455GluVal: 4.455 ± 0.058	0.812GluTrp: 0.812 ± 0.021	3.509GluTyr: 3.509 ± 0.052	0.0GluXaa: 0.0 ± 0.0
Phe	2.893PheAla: 2.893 ± 0.043	0.826PheCys: 0.826 ± 0.023	2.428PheAsp: 2.428 ± 0.04	2.76PheGlu: 2.76 ± 0.044	1.931PhePhe: 1.931 ± 0.04	3.017PheGly: 3.017 ± 0.046	0.879PheHis: 0.879 ± 0.024	2.717PheIle: 2.717 ± 0.047	1.735PheLys: 1.735 ± 0.035	4.19PheLeu: 4.19 ± 0.052	1.157PheMet: 1.157 ± 0.028	1.331PheAsn: 1.331 ± 0.03	1.502PhePro: 1.502 ± 0.029	1.558PheGln: 1.558 ± 0.029	2.111PheArg: 2.111 ± 0.039	2.983PheSer: 2.983 ± 0.047	2.25PheThr: 2.25 ± 0.044	2.628PheVal: 2.628 ± 0.046	0.468PheTrp: 0.468 ± 0.017	1.873PheTyr: 1.873 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	4.985GlyAla: 4.985 ± 0.073	1.29GlyCys: 1.29 ± 0.031	3.36GlyAsp: 3.36 ± 0.049	5.116GlyGlu: 5.116 ± 0.067	3.219GlyPhe: 3.219 ± 0.045	4.922GlyGly: 4.922 ± 0.067	1.268GlyHis: 1.268 ± 0.028	6.118GlyIle: 6.118 ± 0.077	5.325GlyLys: 5.325 ± 0.061	5.818GlyLeu: 5.818 ± 0.066	2.632GlyMet: 2.632 ± 0.046	3.143GlyAsn: 3.143 ± 0.04	1.288GlyPro: 1.288 ± 0.041	2.293GlyGln: 2.293 ± 0.037	3.737GlyArg: 3.737 ± 0.054	4.198GlySer: 4.198 ± 0.053	4.241GlyThr: 4.241 ± 0.058	4.413GlyVal: 4.413 ± 0.058	0.734GlyTrp: 0.734 ± 0.023	3.337GlyTyr: 3.337 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	1.183HisAla: 1.183 ± 0.027	0.339HisCys: 0.339 ± 0.014	0.959HisAsp: 0.959 ± 0.025	1.139HisGlu: 1.139 ± 0.029	0.939HisPhe: 0.939 ± 0.023	1.31HisGly: 1.31 ± 0.029	0.427HisHis: 0.427 ± 0.02	1.424HisIle: 1.424 ± 0.029	0.918HisLys: 0.918 ± 0.025	1.612HisLeu: 1.612 ± 0.03	0.596HisMet: 0.596 ± 0.018	0.653HisAsn: 0.653 ± 0.021	0.917HisPro: 0.917 ± 0.025	0.615HisGln: 0.615 ± 0.018	0.881HisArg: 0.881 ± 0.024	1.0HisSer: 1.0 ± 0.025	1.031HisThr: 1.031 ± 0.023	1.12HisVal: 1.12 ± 0.027	0.189HisTrp: 0.189 ± 0.011	0.847HisTyr: 0.847 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.134IleAla: 5.134 ± 0.065	1.359IleCys: 1.359 ± 0.029	3.654IleAsp: 3.654 ± 0.052	4.702IleGlu: 4.702 ± 0.062	2.912IlePhe: 2.912 ± 0.045	4.804IleGly: 4.804 ± 0.061	1.448IleHis: 1.448 ± 0.03	4.368IleIle: 4.368 ± 0.071	3.6IleLys: 3.6 ± 0.049	7.228IleLeu: 7.228 ± 0.077	1.859IleMet: 1.859 ± 0.036	2.43IleAsn: 2.43 ± 0.035	3.231IlePro: 3.231 ± 0.043	2.64IleGln: 2.64 ± 0.044	4.328IleArg: 4.328 ± 0.059	4.861IleSer: 4.861 ± 0.057	3.742IleThr: 3.742 ± 0.053	4.367IleVal: 4.367 ± 0.055	0.682IleTrp: 0.682 ± 0.023	2.792IleTyr: 2.792 ± 0.042	0.0IleXaa: 0.0 ± 0.0
Lys	4.824LysAla: 4.824 ± 0.064	0.716LysCys: 0.716 ± 0.021	3.616LysAsp: 3.616 ± 0.047	6.615LysGlu: 6.615 ± 0.078	1.758LysPhe: 1.758 ± 0.036	4.006LysGly: 4.006 ± 0.051	1.026LysHis: 1.026 ± 0.026	4.433LysIle: 4.433 ± 0.059	6.014LysLys: 6.014 ± 0.076	5.175LysLeu: 5.175 ± 0.062	1.995LysMet: 1.995 ± 0.036	3.351LysAsn: 3.351 ± 0.05	1.956LysPro: 1.956 ± 0.037	2.449LysGln: 2.449 ± 0.043	3.417LysArg: 3.417 ± 0.052	3.204LysSer: 3.204 ± 0.041	3.48LysThr: 3.48 ± 0.044	3.734LysVal: 3.734 ± 0.051	0.652LysTrp: 0.652 ± 0.02	2.844LysTyr: 2.844 ± 0.042	0.0LysXaa: 0.0 ± 0.0
Leu	6.625LeuAla: 6.625 ± 0.08	1.645LeuCys: 1.645 ± 0.032	5.099LeuAsp: 5.099 ± 0.059	6.853LeuGlu: 6.853 ± 0.076	3.901LeuPhe: 3.901 ± 0.057	5.809LeuGly: 5.809 ± 0.065	1.651LeuHis: 1.651 ± 0.034	5.64LeuIle: 5.64 ± 0.077	5.949LeuLys: 5.949 ± 0.066	8.866LeuLeu: 8.866 ± 0.107	2.614LeuMet: 2.614 ± 0.04	3.711LeuAsn: 3.711 ± 0.051	3.612LeuPro: 3.612 ± 0.049	3.076LeuGln: 3.076 ± 0.047	4.305LeuArg: 4.305 ± 0.053	6.2LeuSer: 6.2 ± 0.068	4.917LeuThr: 4.917 ± 0.058	5.153LeuVal: 5.153 ± 0.071	0.843LeuTrp: 0.843 ± 0.023	3.615LeuTyr: 3.615 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	2.343MetAla: 2.343 ± 0.045	0.383MetCys: 0.383 ± 0.017	1.985MetAsp: 1.985 ± 0.04	2.811MetGlu: 2.811 ± 0.047	1.054MetPhe: 1.054 ± 0.025	2.062MetGly: 2.062 ± 0.038	0.502MetHis: 0.502 ± 0.019	2.128MetIle: 2.128 ± 0.039	2.536MetLys: 2.536 ± 0.043	2.906MetLeu: 2.906 ± 0.043	0.98MetMet: 0.98 ± 0.027	1.522MetAsn: 1.522 ± 0.033	1.186MetPro: 1.186 ± 0.031	1.167MetGln: 1.167 ± 0.028	1.428MetArg: 1.428 ± 0.027	1.866MetSer: 1.866 ± 0.057	1.657MetThr: 1.657 ± 0.033	1.813MetVal: 1.813 ± 0.033	0.24MetTrp: 0.24 ± 0.013	0.948MetTyr: 0.948 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.924AsnAla: 2.924 ± 0.046	0.592AsnCys: 0.592 ± 0.02	1.911AsnAsp: 1.911 ± 0.038	2.731AsnGlu: 2.731 ± 0.044	1.496AsnPhe: 1.496 ± 0.034	3.392AsnGly: 3.392 ± 0.047	0.835AsnHis: 0.835 ± 0.025	3.07AsnIle: 3.07 ± 0.048	2.11AsnLys: 2.11 ± 0.038	3.662AsnLeu: 3.662 ± 0.048	1.301AsnMet: 1.301 ± 0.026	1.51AsnAsn: 1.51 ± 0.035	2.0AsnPro: 2.0 ± 0.035	1.513AsnGln: 1.513 ± 0.03	2.263AsnArg: 2.263 ± 0.038	2.126AsnSer: 2.126 ± 0.04	2.181AsnThr: 2.181 ± 0.035	2.532AsnVal: 2.532 ± 0.04	0.435AsnTrp: 0.435 ± 0.018	1.775AsnTyr: 1.775 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	2.673ProAla: 2.673 ± 0.046	0.48ProCys: 0.48 ± 0.019	2.49ProAsp: 2.49 ± 0.044	3.72ProGlu: 3.72 ± 0.053	1.552ProPhe: 1.552 ± 0.033	2.504ProGly: 2.504 ± 0.044	0.619ProHis: 0.619 ± 0.02	1.964ProIle: 1.964 ± 0.031	1.894ProLys: 1.894 ± 0.039	2.768ProLeu: 2.768 ± 0.046	0.929ProMet: 0.929 ± 0.023	1.156ProAsn: 1.156 ± 0.027	0.871ProPro: 0.871 ± 0.027	1.121ProGln: 1.121 ± 0.03	1.133ProArg: 1.133 ± 0.028	1.755ProSer: 1.755 ± 0.034	1.386ProThr: 1.386 ± 0.029	2.964ProVal: 2.964 ± 0.039	0.357ProTrp: 0.357 ± 0.014	1.532ProTyr: 1.532 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	2.702GlnAla: 2.702 ± 0.047	0.396GlnCys: 0.396 ± 0.015	1.814GlnAsp: 1.814 ± 0.033	3.434GlnGlu: 3.434 ± 0.056	1.259GlnPhe: 1.259 ± 0.025	2.169GlnGly: 2.169 ± 0.046	0.486GlnHis: 0.486 ± 0.017	2.783GlnIle: 2.783 ± 0.046	2.945GlnLys: 2.945 ± 0.05	2.698GlnLeu: 2.698 ± 0.044	1.318GlnMet: 1.318 ± 0.027	1.725GlnAsn: 1.725 ± 0.034	1.012GlnPro: 1.012 ± 0.027	1.256GlnGln: 1.256 ± 0.032	1.609GlnArg: 1.609 ± 0.033	1.764GlnSer: 1.764 ± 0.032	1.817GlnThr: 1.817 ± 0.038	2.065GlnVal: 2.065 ± 0.037	0.351GlnTrp: 0.351 ± 0.016	1.495GlnTyr: 1.495 ± 0.032	0.0GlnXaa: 0.0 ± 0.0
Arg	3.02ArgAla: 3.02 ± 0.046	0.708ArgCys: 0.708 ± 0.023	2.476ArgAsp: 2.476 ± 0.042	4.583ArgGlu: 4.583 ± 0.066	2.183ArgPhe: 2.183 ± 0.043	2.775ArgGly: 2.775 ± 0.051	0.969ArgHis: 0.969 ± 0.026	4.063ArgIle: 4.063 ± 0.057	4.081ArgLys: 4.081 ± 0.05	4.626ArgLeu: 4.626 ± 0.06	1.819ArgMet: 1.819 ± 0.036	2.358ArgAsn: 2.358 ± 0.047	1.533ArgPro: 1.533 ± 0.037	2.142ArgGln: 2.142 ± 0.043	2.971ArgArg: 2.971 ± 0.052	2.484ArgSer: 2.484 ± 0.045	2.501ArgThr: 2.501 ± 0.036	2.741ArgVal: 2.741 ± 0.042	0.484ArgTrp: 0.484 ± 0.017	2.228ArgTyr: 2.228 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	4.429SerAla: 4.429 ± 0.064	0.87SerCys: 0.87 ± 0.022	3.258SerAsp: 3.258 ± 0.068	4.158SerGlu: 4.158 ± 0.056	2.566SerPhe: 2.566 ± 0.041	5.346SerGly: 5.346 ± 0.068	1.023SerHis: 1.023 ± 0.025	3.954SerIle: 3.954 ± 0.051	3.006SerLys: 3.006 ± 0.048	5.09SerLeu: 5.09 ± 0.063	1.996SerMet: 1.996 ± 0.056	1.98SerAsn: 1.98 ± 0.036	1.823SerPro: 1.823 ± 0.032	1.871SerGln: 1.871 ± 0.039	3.218SerArg: 3.218 ± 0.049	3.386SerSer: 3.386 ± 0.063	2.549SerThr: 2.549 ± 0.045	4.113SerVal: 4.113 ± 0.053	0.613SerTrp: 0.613 ± 0.018	2.414SerTyr: 2.414 ± 0.035	0.0SerXaa: 0.0 ± 0.0
Thr	4.633ThrAla: 4.633 ± 0.067	0.659ThrCys: 0.659 ± 0.017	3.087ThrAsp: 3.087 ± 0.045	3.984ThrGlu: 3.984 ± 0.061	2.069ThrPhe: 2.069 ± 0.041	4.508ThrGly: 4.508 ± 0.057	0.809ThrHis: 0.809 ± 0.024	3.47ThrIle: 3.47 ± 0.048	2.715ThrLys: 2.715 ± 0.043	4.443ThrLeu: 4.443 ± 0.055	1.389ThrMet: 1.389 ± 0.028	1.773ThrAsn: 1.773 ± 0.037	2.026ThrPro: 2.026 ± 0.04	1.418ThrGln: 1.418 ± 0.028	2.158ThrArg: 2.158 ± 0.033	2.702ThrSer: 2.702 ± 0.045	2.348ThrThr: 2.348 ± 0.045	4.191ThrVal: 4.191 ± 0.05	0.503ThrTrp: 0.503 ± 0.021	2.001ThrTyr: 2.001 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	3.894ValAla: 3.894 ± 0.053	1.215ValCys: 1.215 ± 0.029	3.396ValAsp: 3.396 ± 0.049	4.463ValGlu: 4.463 ± 0.065	2.95ValPhe: 2.95 ± 0.051	3.757ValGly: 3.757 ± 0.056	1.121ValHis: 1.121 ± 0.027	4.807ValIle: 4.807 ± 0.058	4.082ValLys: 4.082 ± 0.053	6.541ValLeu: 6.541 ± 0.074	2.077ValMet: 2.077 ± 0.039	2.707ValAsn: 2.707 ± 0.047	2.536ValPro: 2.536 ± 0.045	2.067ValGln: 2.067 ± 0.036	3.328ValArg: 3.328 ± 0.052	4.508ValSer: 4.508 ± 0.059	3.69ValThr: 3.69 ± 0.052	4.067ValVal: 4.067 ± 0.064	0.7ValTrp: 0.7 ± 0.02	2.706ValTyr: 2.706 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	0.555TrpAla: 0.555 ± 0.019	0.192TrpCys: 0.192 ± 0.01	0.623TrpAsp: 0.623 ± 0.021	0.769TrpGlu: 0.769 ± 0.02	0.419TrpPhe: 0.419 ± 0.016	0.726TrpGly: 0.726 ± 0.02	0.221TrpHis: 0.221 ± 0.011	0.773TrpIle: 0.773 ± 0.021	0.829TrpLys: 0.829 ± 0.028	0.952TrpLeu: 0.952 ± 0.026	0.354TrpMet: 0.354 ± 0.016	0.58TrpAsn: 0.58 ± 0.019	0.223TrpPro: 0.223 ± 0.011	0.41TrpGln: 0.41 ± 0.015	0.443TrpArg: 0.443 ± 0.018	0.519TrpSer: 0.519 ± 0.019	0.472TrpThr: 0.472 ± 0.018	0.548TrpVal: 0.548 ± 0.018	0.137TrpTrp: 0.137 ± 0.01	0.423TrpTyr: 0.423 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.805TyrAla: 2.805 ± 0.043	0.726TyrCys: 0.726 ± 0.018	2.543TyrAsp: 2.543 ± 0.042	3.21TyrGlu: 3.21 ± 0.051	1.881TyrPhe: 1.881 ± 0.035	3.135TyrGly: 3.135 ± 0.05	1.012TyrHis: 1.012 ± 0.026	2.893TyrIle: 2.893 ± 0.042	2.158TyrLys: 2.158 ± 0.036	3.985TyrLeu: 3.985 ± 0.053	1.171TyrMet: 1.171 ± 0.023	1.636TyrAsn: 1.636 ± 0.034	1.516TyrPro: 1.516 ± 0.036	1.75TyrGln: 1.75 ± 0.032	2.385TyrArg: 2.385 ± 0.044	2.347TyrSer: 2.347 ± 0.041	2.313TyrThr: 2.313 ± 0.037	2.58TyrVal: 2.58 ± 0.04	0.445TyrTrp: 0.445 ± 0.015	1.919TyrTyr: 1.919 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5263 proteins (1644781 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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