Amino acid dipepetide frequency for Mycobacterium phage Kumao

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.486AlaAla: 9.486 ± 1.098
0.519AlaCys: 0.519 ± 0.213
5.191AlaAsp: 5.191 ± 0.496
7.881AlaGlu: 7.881 ± 0.831
2.548AlaPhe: 2.548 ± 0.409
6.56AlaGly: 6.56 ± 0.994
2.548AlaHis: 2.548 ± 0.387
5.003AlaIle: 5.003 ± 0.465
4.247AlaLys: 4.247 ± 0.484
8.259AlaLeu: 8.259 ± 0.84
3.02AlaMet: 3.02 ± 0.419
3.634AlaAsn: 3.634 ± 0.444
3.634AlaPro: 3.634 ± 0.506
4.295AlaGln: 4.295 ± 0.419
6.56AlaArg: 6.56 ± 0.615
4.153AlaSer: 4.153 ± 0.495
5.522AlaThr: 5.522 ± 0.68
7.74AlaVal: 7.74 ± 0.692
2.171AlaTrp: 2.171 ± 0.311
2.832AlaTyr: 2.832 ± 0.352
0.0AlaXaa: 0.0 ± 0.0
Cys
0.614CysAla: 0.614 ± 0.17
0.283CysCys: 0.283 ± 0.127
0.566CysAsp: 0.566 ± 0.169
0.614CysGlu: 0.614 ± 0.196
0.519CysPhe: 0.519 ± 0.174
1.321CysGly: 1.321 ± 0.31
0.283CysHis: 0.283 ± 0.12
0.33CysIle: 0.33 ± 0.157
0.472CysLys: 0.472 ± 0.182
0.802CysLeu: 0.802 ± 0.195
0.142CysMet: 0.142 ± 0.078
0.33CysAsn: 0.33 ± 0.126
0.708CysPro: 0.708 ± 0.201
0.33CysGln: 0.33 ± 0.127
0.897CysArg: 0.897 ± 0.238
0.283CysSer: 0.283 ± 0.127
0.378CysThr: 0.378 ± 0.135
0.283CysVal: 0.283 ± 0.142
0.283CysTrp: 0.283 ± 0.114
0.189CysTyr: 0.189 ± 0.094
0.0CysXaa: 0.0 ± 0.0
Asp
6.135AspAla: 6.135 ± 0.635
0.708AspCys: 0.708 ± 0.198
4.955AspAsp: 4.955 ± 0.641
5.003AspGlu: 5.003 ± 0.501
1.935AspPhe: 1.935 ± 0.366
6.135AspGly: 6.135 ± 0.585
1.321AspHis: 1.321 ± 0.236
3.445AspIle: 3.445 ± 0.399
3.02AspLys: 3.02 ± 0.387
5.097AspLeu: 5.097 ± 0.504
1.274AspMet: 1.274 ± 0.222
2.407AspAsn: 2.407 ± 0.355
3.728AspPro: 3.728 ± 0.449
1.605AspGln: 1.605 ± 0.248
4.436AspArg: 4.436 ± 0.478
2.973AspSer: 2.973 ± 0.363
2.973AspThr: 2.973 ± 0.415
4.389AspVal: 4.389 ± 0.484
1.652AspTrp: 1.652 ± 0.269
2.596AspTyr: 2.596 ± 0.457
0.0AspXaa: 0.0 ± 0.0
Glu
7.881GluAla: 7.881 ± 0.707
0.897GluCys: 0.897 ± 0.242
4.908GluAsp: 4.908 ± 0.499
4.342GluGlu: 4.342 ± 0.535
2.407GluPhe: 2.407 ± 0.34
4.483GluGly: 4.483 ± 0.432
1.463GluHis: 1.463 ± 0.31
3.681GluIle: 3.681 ± 0.527
2.643GluLys: 2.643 ± 0.489
6.654GluLeu: 6.654 ± 0.625
1.652GluMet: 1.652 ± 0.385
2.077GluAsn: 2.077 ± 0.406
3.256GluPro: 3.256 ± 0.533
2.454GluGln: 2.454 ± 0.405
5.05GluArg: 5.05 ± 0.632
3.917GluSer: 3.917 ± 0.376
2.973GluThr: 2.973 ± 0.32
4.861GluVal: 4.861 ± 0.692
2.265GluTrp: 2.265 ± 0.382
2.407GluTyr: 2.407 ± 0.334
0.0GluXaa: 0.0 ± 0.0
Phe
2.643PheAla: 2.643 ± 0.355
0.425PheCys: 0.425 ± 0.141
2.454PheAsp: 2.454 ± 0.337
1.888PheGlu: 1.888 ± 0.291
0.519PhePhe: 0.519 ± 0.152
2.737PheGly: 2.737 ± 0.518
0.472PheHis: 0.472 ± 0.158
1.321PheIle: 1.321 ± 0.232
1.652PheLys: 1.652 ± 0.303
2.171PheLeu: 2.171 ± 0.333
0.661PheMet: 0.661 ± 0.173
1.51PheAsn: 1.51 ± 0.251
1.699PhePro: 1.699 ± 0.225
1.038PheGln: 1.038 ± 0.236
1.935PheArg: 1.935 ± 0.269
2.171PheSer: 2.171 ± 0.342
1.416PheThr: 1.416 ± 0.272
2.218PheVal: 2.218 ± 0.396
0.755PheTrp: 0.755 ± 0.213
1.038PheTyr: 1.038 ± 0.214
0.0PheXaa: 0.0 ± 0.0
Gly
6.324GlyAla: 6.324 ± 0.818
0.472GlyCys: 0.472 ± 0.14
5.899GlyAsp: 5.899 ± 0.602
6.324GlyGlu: 6.324 ± 0.566
2.548GlyPhe: 2.548 ± 0.451
7.504GlyGly: 7.504 ± 0.998
1.557GlyHis: 1.557 ± 0.386
3.917GlyIle: 3.917 ± 0.562
3.587GlyLys: 3.587 ± 0.37
6.466GlyLeu: 6.466 ± 0.952
1.699GlyMet: 1.699 ± 0.304
3.351GlyAsn: 3.351 ± 0.523
3.162GlyPro: 3.162 ± 0.428
2.596GlyGln: 2.596 ± 0.339
4.531GlyArg: 4.531 ± 0.515
5.097GlySer: 5.097 ± 0.996
5.522GlyThr: 5.522 ± 0.532
6.324GlyVal: 6.324 ± 0.562
2.077GlyTrp: 2.077 ± 0.344
2.926GlyTyr: 2.926 ± 0.353
0.0GlyXaa: 0.0 ± 0.0
His
1.51HisAla: 1.51 ± 0.285
0.236HisCys: 0.236 ± 0.112
1.274HisAsp: 1.274 ± 0.314
1.935HisGlu: 1.935 ± 0.335
1.133HisPhe: 1.133 ± 0.207
1.51HisGly: 1.51 ± 0.311
0.519HisHis: 0.519 ± 0.181
1.133HisIle: 1.133 ± 0.216
1.227HisLys: 1.227 ± 0.267
2.171HisLeu: 2.171 ± 0.269
0.33HisMet: 0.33 ± 0.105
0.802HisAsn: 0.802 ± 0.194
1.557HisPro: 1.557 ± 0.286
0.802HisGln: 0.802 ± 0.205
1.935HisArg: 1.935 ± 0.376
0.802HisSer: 0.802 ± 0.181
1.227HisThr: 1.227 ± 0.231
1.274HisVal: 1.274 ± 0.258
0.614HisTrp: 0.614 ± 0.189
0.614HisTyr: 0.614 ± 0.166
0.0HisXaa: 0.0 ± 0.0
Ile
5.569IleAla: 5.569 ± 0.534
0.897IleCys: 0.897 ± 0.22
3.776IleAsp: 3.776 ± 0.405
3.115IleGlu: 3.115 ± 0.348
1.369IlePhe: 1.369 ± 0.255
3.587IleGly: 3.587 ± 0.455
1.416IleHis: 1.416 ± 0.291
2.265IleIle: 2.265 ± 0.314
1.557IleLys: 1.557 ± 0.197
3.587IleLeu: 3.587 ± 0.362
0.708IleMet: 0.708 ± 0.187
2.124IleAsn: 2.124 ± 0.314
2.832IlePro: 2.832 ± 0.308
1.699IleGln: 1.699 ± 0.302
3.492IleArg: 3.492 ± 0.399
1.982IleSer: 1.982 ± 0.263
2.407IleThr: 2.407 ± 0.341
3.445IleVal: 3.445 ± 0.458
1.038IleTrp: 1.038 ± 0.233
1.038IleTyr: 1.038 ± 0.239
0.0IleXaa: 0.0 ± 0.0
Lys
4.578LysAla: 4.578 ± 0.523
0.378LysCys: 0.378 ± 0.125
2.548LysAsp: 2.548 ± 0.38
2.218LysGlu: 2.218 ± 0.343
0.991LysPhe: 0.991 ± 0.201
4.2LysGly: 4.2 ± 0.546
0.755LysHis: 0.755 ± 0.219
1.888LysIle: 1.888 ± 0.395
1.605LysLys: 1.605 ± 0.295
3.492LysLeu: 3.492 ± 0.482
0.614LysMet: 0.614 ± 0.177
1.935LysAsn: 1.935 ± 0.313
2.454LysPro: 2.454 ± 0.446
2.36LysGln: 2.36 ± 0.357
3.209LysArg: 3.209 ± 0.426
2.265LysSer: 2.265 ± 0.367
2.313LysThr: 2.313 ± 0.306
4.012LysVal: 4.012 ± 0.566
1.038LysTrp: 1.038 ± 0.22
1.463LysTyr: 1.463 ± 0.245
0.0LysXaa: 0.0 ± 0.0
Leu
9.061LeuAla: 9.061 ± 0.654
0.661LeuCys: 0.661 ± 0.213
5.333LeuAsp: 5.333 ± 0.533
5.286LeuGlu: 5.286 ± 0.683
2.643LeuPhe: 2.643 ± 0.391
6.277LeuGly: 6.277 ± 0.685
1.935LeuHis: 1.935 ± 0.355
4.153LeuIle: 4.153 ± 0.388
4.012LeuLys: 4.012 ± 0.453
4.955LeuLeu: 4.955 ± 0.436
2.029LeuMet: 2.029 ± 0.332
3.209LeuAsn: 3.209 ± 0.584
4.955LeuPro: 4.955 ± 0.609
2.973LeuGln: 2.973 ± 0.331
5.003LeuArg: 5.003 ± 0.563
4.153LeuSer: 4.153 ± 0.54
4.531LeuThr: 4.531 ± 0.442
4.719LeuVal: 4.719 ± 0.435
1.793LeuTrp: 1.793 ± 0.331
1.746LeuTyr: 1.746 ± 0.314
0.0LeuXaa: 0.0 ± 0.0
Met
3.587MetAla: 3.587 ± 0.508
0.283MetCys: 0.283 ± 0.132
0.944MetAsp: 0.944 ± 0.193
1.463MetGlu: 1.463 ± 0.243
0.519MetPhe: 0.519 ± 0.139
1.18MetGly: 1.18 ± 0.195
0.33MetHis: 0.33 ± 0.129
1.038MetIle: 1.038 ± 0.258
0.991MetLys: 0.991 ± 0.25
1.321MetLeu: 1.321 ± 0.235
0.472MetMet: 0.472 ± 0.181
0.755MetAsn: 0.755 ± 0.2
1.369MetPro: 1.369 ± 0.268
0.566MetGln: 0.566 ± 0.234
1.51MetArg: 1.51 ± 0.291
1.982MetSer: 1.982 ± 0.322
2.171MetThr: 2.171 ± 0.262
0.944MetVal: 0.944 ± 0.179
0.283MetTrp: 0.283 ± 0.11
0.519MetTyr: 0.519 ± 0.142
0.0MetXaa: 0.0 ± 0.0
Asn
3.115AsnAla: 3.115 ± 0.387
0.378AsnCys: 0.378 ± 0.128
2.218AsnAsp: 2.218 ± 0.298
2.171AsnGlu: 2.171 ± 0.385
1.085AsnPhe: 1.085 ± 0.232
3.776AsnGly: 3.776 ± 0.498
1.133AsnHis: 1.133 ± 0.231
1.935AsnIle: 1.935 ± 0.312
1.038AsnLys: 1.038 ± 0.242
3.068AsnLeu: 3.068 ± 0.398
0.755AsnMet: 0.755 ± 0.195
1.416AsnAsn: 1.416 ± 0.281
2.973AsnPro: 2.973 ± 0.388
1.416AsnGln: 1.416 ± 0.312
2.218AsnArg: 2.218 ± 0.298
1.557AsnSer: 1.557 ± 0.301
2.029AsnThr: 2.029 ± 0.346
2.69AsnVal: 2.69 ± 0.394
0.897AsnTrp: 0.897 ± 0.202
1.369AsnTyr: 1.369 ± 0.29
0.0AsnXaa: 0.0 ± 0.0
Pro
4.436ProAla: 4.436 ± 0.62
0.378ProCys: 0.378 ± 0.128
3.304ProAsp: 3.304 ± 0.374
3.87ProGlu: 3.87 ± 0.44
1.982ProPhe: 1.982 ± 0.315
5.144ProGly: 5.144 ± 0.908
1.038ProHis: 1.038 ± 0.193
2.218ProIle: 2.218 ± 0.217
2.313ProLys: 2.313 ± 0.398
3.964ProLeu: 3.964 ± 0.474
0.944ProMet: 0.944 ± 0.238
1.888ProAsn: 1.888 ± 0.332
2.926ProPro: 2.926 ± 0.383
2.124ProGln: 2.124 ± 0.417
2.879ProArg: 2.879 ± 0.341
2.784ProSer: 2.784 ± 0.495
3.256ProThr: 3.256 ± 0.51
3.87ProVal: 3.87 ± 0.454
1.18ProTrp: 1.18 ± 0.239
1.605ProTyr: 1.605 ± 0.287
0.0ProXaa: 0.0 ± 0.0
Gln
4.059GlnAla: 4.059 ± 0.525
0.236GlnCys: 0.236 ± 0.093
2.36GlnAsp: 2.36 ± 0.324
2.69GlnGlu: 2.69 ± 0.349
1.416GlnPhe: 1.416 ± 0.32
2.218GlnGly: 2.218 ± 0.321
0.566GlnHis: 0.566 ± 0.198
1.841GlnIle: 1.841 ± 0.296
1.699GlnLys: 1.699 ± 0.258
2.737GlnLeu: 2.737 ± 0.413
0.944GlnMet: 0.944 ± 0.197
1.227GlnAsn: 1.227 ± 0.265
1.793GlnPro: 1.793 ± 0.3
1.699GlnGln: 1.699 ± 0.375
3.445GlnArg: 3.445 ± 0.427
2.124GlnSer: 2.124 ± 0.283
1.227GlnThr: 1.227 ± 0.224
2.879GlnVal: 2.879 ± 0.31
1.133GlnTrp: 1.133 ± 0.258
0.849GlnTyr: 0.849 ± 0.201
0.0GlnXaa: 0.0 ± 0.0
Arg
5.239ArgAla: 5.239 ± 0.51
0.991ArgCys: 0.991 ± 0.208
3.492ArgAsp: 3.492 ± 0.287
5.427ArgGlu: 5.427 ± 0.683
2.077ArgPhe: 2.077 ± 0.311
4.672ArgGly: 4.672 ± 0.468
1.463ArgHis: 1.463 ± 0.306
3.256ArgIle: 3.256 ± 0.419
3.681ArgLys: 3.681 ± 0.617
5.569ArgLeu: 5.569 ± 0.566
2.171ArgMet: 2.171 ± 0.311
2.265ArgAsn: 2.265 ± 0.431
2.643ArgPro: 2.643 ± 0.415
2.69ArgGln: 2.69 ± 0.352
5.239ArgArg: 5.239 ± 0.712
2.784ArgSer: 2.784 ± 0.38
3.823ArgThr: 3.823 ± 0.405
5.003ArgVal: 5.003 ± 0.544
2.265ArgTrp: 2.265 ± 0.385
2.36ArgTyr: 2.36 ± 0.344
0.0ArgXaa: 0.0 ± 0.0
Ser
5.05SerAla: 5.05 ± 0.552
0.425SerCys: 0.425 ± 0.151
3.492SerAsp: 3.492 ± 0.467
3.587SerGlu: 3.587 ± 0.402
1.605SerPhe: 1.605 ± 0.263
5.522SerGly: 5.522 ± 0.794
1.274SerHis: 1.274 ± 0.301
2.313SerIle: 2.313 ± 0.319
2.454SerLys: 2.454 ± 0.337
4.247SerLeu: 4.247 ± 0.593
0.661SerMet: 0.661 ± 0.167
1.841SerAsn: 1.841 ± 0.392
2.784SerPro: 2.784 ± 0.344
1.793SerGln: 1.793 ± 0.292
3.068SerArg: 3.068 ± 0.418
2.643SerSer: 2.643 ± 0.476
2.218SerThr: 2.218 ± 0.354
4.106SerVal: 4.106 ± 0.59
1.557SerTrp: 1.557 ± 0.242
1.51SerTyr: 1.51 ± 0.325
0.0SerXaa: 0.0 ± 0.0
Thr
4.106ThrAla: 4.106 ± 0.427
0.425ThrCys: 0.425 ± 0.178
2.879ThrAsp: 2.879 ± 0.337
3.115ThrGlu: 3.115 ± 0.428
2.029ThrPhe: 2.029 ± 0.389
5.899ThrGly: 5.899 ± 0.711
1.085ThrHis: 1.085 ± 0.202
2.737ThrIle: 2.737 ± 0.4
2.69ThrLys: 2.69 ± 0.329
4.767ThrLeu: 4.767 ± 0.478
0.897ThrMet: 0.897 ± 0.207
1.227ThrAsn: 1.227 ± 0.263
3.728ThrPro: 3.728 ± 0.545
1.982ThrGln: 1.982 ± 0.259
2.643ThrArg: 2.643 ± 0.327
3.209ThrSer: 3.209 ± 0.65
3.068ThrThr: 3.068 ± 0.502
5.097ThrVal: 5.097 ± 0.658
0.849ThrTrp: 0.849 ± 0.23
1.463ThrTyr: 1.463 ± 0.278
0.0ThrXaa: 0.0 ± 0.0
Val
6.938ValAla: 6.938 ± 0.606
0.519ValCys: 0.519 ± 0.167
6.23ValAsp: 6.23 ± 0.585
5.569ValGlu: 5.569 ± 0.633
2.029ValPhe: 2.029 ± 0.37
5.475ValGly: 5.475 ± 0.566
1.699ValHis: 1.699 ± 0.27
3.304ValIle: 3.304 ± 0.313
3.304ValLys: 3.304 ± 0.482
5.191ValLeu: 5.191 ± 0.594
1.935ValMet: 1.935 ± 0.377
2.313ValAsn: 2.313 ± 0.282
3.823ValPro: 3.823 ± 0.43
2.596ValGln: 2.596 ± 0.318
5.097ValArg: 5.097 ± 0.544
4.247ValSer: 4.247 ± 0.445
4.2ValThr: 4.2 ± 0.502
6.607ValVal: 6.607 ± 0.672
1.652ValTrp: 1.652 ± 0.307
2.029ValTyr: 2.029 ± 0.348
0.0ValXaa: 0.0 ± 0.0
Trp
2.501TrpAla: 2.501 ± 0.433
0.378TrpCys: 0.378 ± 0.154
1.746TrpAsp: 1.746 ± 0.377
1.935TrpGlu: 1.935 ± 0.345
0.614TrpPhe: 0.614 ± 0.199
1.321TrpGly: 1.321 ± 0.218
0.897TrpHis: 0.897 ± 0.201
1.18TrpIle: 1.18 ± 0.24
0.708TrpLys: 0.708 ± 0.173
2.501TrpLeu: 2.501 ± 0.387
0.755TrpMet: 0.755 ± 0.153
1.133TrpAsn: 1.133 ± 0.234
0.378TrpPro: 0.378 ± 0.134
0.944TrpGln: 0.944 ± 0.283
1.699TrpArg: 1.699 ± 0.26
1.416TrpSer: 1.416 ± 0.249
1.18TrpThr: 1.18 ± 0.258
2.077TrpVal: 2.077 ± 0.395
0.661TrpTrp: 0.661 ± 0.17
0.519TrpTyr: 0.519 ± 0.162
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.973TyrAla: 2.973 ± 0.413
0.142TyrCys: 0.142 ± 0.081
2.218TyrAsp: 2.218 ± 0.327
1.841TyrGlu: 1.841 ± 0.307
0.755TyrPhe: 0.755 ± 0.178
2.36TyrGly: 2.36 ± 0.43
0.849TyrHis: 0.849 ± 0.276
0.944TyrIle: 0.944 ± 0.207
1.416TyrLys: 1.416 ± 0.236
2.407TyrLeu: 2.407 ± 0.318
0.519TyrMet: 0.519 ± 0.131
1.793TyrAsn: 1.793 ± 0.395
1.699TyrPro: 1.699 ± 0.311
1.18TyrGln: 1.18 ± 0.283
2.313TyrArg: 2.313 ± 0.344
1.605TyrSer: 1.605 ± 0.264
1.463TyrThr: 1.463 ± 0.274
2.218TyrVal: 2.218 ± 0.369
0.378TyrTrp: 0.378 ± 0.158
1.416TyrTyr: 1.416 ± 0.231
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 115 proteins (21190 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski