Takifugu flavidus (sansaifugu)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei;

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29076 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C6NUM2|A0A5C6NUM2_9TELE Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 OS=Takifugu flavidus OX=433684 GN=D4764_18G0013140 PE=4 SV=1
MM1 pKa = 6.6QQVEE5 pKa = 4.59EE6 pKa = 4.37AAMTTQPSPSEE17 pKa = 3.75QAPPLTEE24 pKa = 3.93SPEE27 pKa = 4.37AKK29 pKa = 10.18DD30 pKa = 3.43HH31 pKa = 6.24QQDD34 pKa = 3.75QNHH37 pKa = 5.87LTVISYY43 pKa = 10.76DD44 pKa = 3.92SEE46 pKa = 4.31PSGAGAPVQSTSPKK60 pKa = 7.82PAGRR64 pKa = 11.84SEE66 pKa = 3.62EE67 pKa = 4.17RR68 pKa = 11.84ANGRR72 pKa = 11.84PGLGSRR78 pKa = 11.84SSSVAGGSPRR88 pKa = 11.84PSLARR93 pKa = 11.84QPSTLTEE100 pKa = 3.9AALDD104 pKa = 3.9GSKK107 pKa = 10.64PRR109 pKa = 11.84DD110 pKa = 3.64YY111 pKa = 11.33LFLAILSCFCPLWPINIVALAFSVMSRR138 pKa = 11.84NSLQQGNVDD147 pKa = 3.32GARR150 pKa = 11.84RR151 pKa = 11.84LGRR154 pKa = 11.84NAMILSIVSILGGIAAIAATIALNWGPTDD183 pKa = 4.28APTGLCSTDD192 pKa = 3.58TSCLTIPWSQPSDD205 pKa = 3.76CPTDD209 pKa = 3.75PEE211 pKa = 4.51LTTDD215 pKa = 4.33AGITTDD221 pKa = 4.36PGLTTDD227 pKa = 5.04AGITTDD233 pKa = 4.36PGLTTDD239 pKa = 4.99AGLTTDD245 pKa = 4.96PEE247 pKa = 4.46LTTDD251 pKa = 4.57AEE253 pKa = 4.36LSGCFTDD260 pKa = 5.92PEE262 pKa = 4.54LTTDD266 pKa = 4.01AGVSGCFTDD275 pKa = 5.53PEE277 pKa = 4.52LTTDD281 pKa = 3.93PGLNTDD287 pKa = 4.42PEE289 pKa = 4.53LTTDD293 pKa = 4.28AGLTTDD299 pKa = 4.96PEE301 pKa = 4.46LTTDD305 pKa = 4.35AEE307 pKa = 4.49LTTEE311 pKa = 4.81AEE313 pKa = 4.17LSGCFTDD320 pKa = 5.92PEE322 pKa = 4.49LTTDD326 pKa = 4.57AEE328 pKa = 4.36LSGCFTDD335 pKa = 5.92PEE337 pKa = 4.54LTTDD341 pKa = 4.01AGVSGCFTDD350 pKa = 5.53PEE352 pKa = 4.54LTTDD356 pKa = 4.01AGVSGCFTDD365 pKa = 5.53PEE367 pKa = 4.54LTTDD371 pKa = 4.01AGVSGCFTDD380 pKa = 5.53PEE382 pKa = 4.49LTTDD386 pKa = 4.57AEE388 pKa = 4.36LSGCFTDD395 pKa = 5.92PEE397 pKa = 4.54LTTDD401 pKa = 4.01AGVSGCFTDD410 pKa = 4.54PEE412 pKa = 4.48LPKK415 pKa = 10.71LFSILPADD423 pKa = 3.67VTVCYY428 pKa = 10.32HH429 pKa = 6.95LLL431 pKa = 3.8

Molecular weight: 44.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C6MT66|A0A5C6MT66_9TELE Major facilitator superfamily domain-containing protein 5 OS=Takifugu flavidus OX=433684 GN=D4764_08G0006570 PE=3 SV=1
MM1 pKa = 7.0PAKK4 pKa = 10.27RR5 pKa = 11.84RR6 pKa = 11.84ISKK9 pKa = 10.66AKK11 pKa = 8.41TSTVRR16 pKa = 11.84EE17 pKa = 4.24RR18 pKa = 11.84ILQAVSNSRR27 pKa = 11.84EE28 pKa = 4.06RR29 pKa = 11.84NGVPLSTLKK38 pKa = 10.67KK39 pKa = 8.97ALAAGGYY46 pKa = 10.31DD47 pKa = 3.42VVKK50 pKa = 10.8NKK52 pKa = 10.58ASVKK56 pKa = 9.45IAIRR60 pKa = 11.84NLLNRR65 pKa = 11.84GALVQTRR72 pKa = 11.84GFGASAAFKK81 pKa = 10.31INKK84 pKa = 8.54NVAGAIAKK92 pKa = 10.02RR93 pKa = 11.84PRR95 pKa = 11.84GKK97 pKa = 10.14KK98 pKa = 9.04AGRR101 pKa = 11.84RR102 pKa = 11.84GRR104 pKa = 11.84KK105 pKa = 8.35RR106 pKa = 11.84AARR109 pKa = 11.84RR110 pKa = 11.84PGKK113 pKa = 9.99KK114 pKa = 9.51GRR116 pKa = 11.84ARR118 pKa = 11.84KK119 pKa = 9.14RR120 pKa = 11.84AGAKK124 pKa = 9.71KK125 pKa = 9.4SRR127 pKa = 11.84KK128 pKa = 8.3RR129 pKa = 11.84GKK131 pKa = 9.99RR132 pKa = 11.84SRR134 pKa = 11.84KK135 pKa = 9.05RR136 pKa = 11.84AAKK139 pKa = 9.83RR140 pKa = 11.84PKK142 pKa = 10.01RR143 pKa = 11.84SAKK146 pKa = 8.77GRR148 pKa = 11.84KK149 pKa = 8.92RR150 pKa = 11.84RR151 pKa = 11.84GKK153 pKa = 9.66KK154 pKa = 9.6AAGKK158 pKa = 9.77RR159 pKa = 11.84KK160 pKa = 9.21SRR162 pKa = 11.84KK163 pKa = 9.35AGTKK167 pKa = 8.8RR168 pKa = 11.84RR169 pKa = 11.84RR170 pKa = 11.84RR171 pKa = 3.55

Molecular weight: 18.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

29076

0

29076

14023189

8

23532

482.3

53.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.684 ± 0.014

2.273 ± 0.011

5.044 ± 0.011

6.853 ± 0.02

3.473 ± 0.01

6.506 ± 0.02

2.73 ± 0.011

4.157 ± 0.011

5.262 ± 0.016

9.772 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.293 ± 0.007

3.598 ± 0.012

5.91 ± 0.02

4.69 ± 0.016

6.211 ± 0.016

8.824 ± 0.019

5.529 ± 0.013

6.44 ± 0.014

1.241 ± 0.005

2.51 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski