Lucerne transient streak virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Sobemovirus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0MN56|L0MN56_9VIRU N-terminal protein OS=Lucerne transient streak virus OX=12470 PE=4 SV=1
MM1 pKa = 7.64PSVIVEE7 pKa = 4.51CYY9 pKa = 7.1TTDD12 pKa = 2.9SHH14 pKa = 8.52RR15 pKa = 11.84DD16 pKa = 3.11IPLFSQKK23 pKa = 8.69IWFDD27 pKa = 3.51NVDD30 pKa = 4.34EE31 pKa = 4.38LTSDD35 pKa = 3.5YY36 pKa = 10.96VRR38 pKa = 11.84VYY40 pKa = 10.49RR41 pKa = 11.84AVRR44 pKa = 11.84DD45 pKa = 3.79YY46 pKa = 11.87SKK48 pKa = 11.12VDD50 pKa = 3.64YY51 pKa = 11.07IEE53 pKa = 4.62VEE55 pKa = 4.07LRR57 pKa = 11.84CNFCHH62 pKa = 6.66YY63 pKa = 10.32YY64 pKa = 10.09NSRR67 pKa = 11.84GQIVQRR73 pKa = 11.84YY74 pKa = 8.16LGCKK78 pKa = 9.69DD79 pKa = 3.26VEE81 pKa = 4.17IRR83 pKa = 11.84NISLPDD89 pKa = 3.68NDD91 pKa = 4.64PYY93 pKa = 11.24TYY95 pKa = 9.83TVYY98 pKa = 10.88SVDD101 pKa = 3.52CAICRR106 pKa = 11.84KK107 pKa = 9.79IPTEE111 pKa = 4.23SEE113 pKa = 4.32DD114 pKa = 3.8EE115 pKa = 4.27SDD117 pKa = 4.24SSFF120 pKa = 3.63

Molecular weight: 14.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0MPW1|L0MPW1_9VIRU Capsid protein OS=Lucerne transient streak virus OX=12470 PE=3 SV=1
MM1 pKa = 6.65VAKK4 pKa = 9.86TRR6 pKa = 11.84RR7 pKa = 11.84ANRR10 pKa = 11.84KK11 pKa = 8.93KK12 pKa = 10.4KK13 pKa = 10.44RR14 pKa = 11.84NNNTQRR20 pKa = 11.84RR21 pKa = 11.84TTEE24 pKa = 3.88PNRR27 pKa = 11.84QLATARR33 pKa = 11.84RR34 pKa = 11.84VTIPAATSVVVRR46 pKa = 11.84KK47 pKa = 9.61SSPVIRR53 pKa = 11.84SDD55 pKa = 2.91RR56 pKa = 11.84KK57 pKa = 10.45GIQVCNTEE65 pKa = 5.1FIRR68 pKa = 11.84SFDD71 pKa = 3.88LNAGFTVGSLPCTPGTMPWLSRR93 pKa = 11.84MAASWGRR100 pKa = 11.84WKK102 pKa = 10.37WNSLRR107 pKa = 11.84FTYY110 pKa = 10.26IPAAPSSTQGTVAMGFLYY128 pKa = 10.6DD129 pKa = 4.97ALDD132 pKa = 3.93SLPSNLASMSSLDD145 pKa = 3.59GFTTGAVWSGCEE157 pKa = 3.75GSAYY161 pKa = 10.07LAKK164 pKa = 10.32PSMRR168 pKa = 11.84DD169 pKa = 2.9IPGAISTCLDD179 pKa = 3.46IDD181 pKa = 4.02PDD183 pKa = 3.41WRR185 pKa = 11.84QKK187 pKa = 7.83RR188 pKa = 11.84WRR190 pKa = 11.84YY191 pKa = 7.65ITALEE196 pKa = 4.01LAALSKK202 pKa = 10.83PDD204 pKa = 3.77FNIYY208 pKa = 10.54APARR212 pKa = 11.84LAIAAANSSINIASVGTLYY231 pKa = 9.99ATYY234 pKa = 9.79EE235 pKa = 4.1VEE237 pKa = 4.82LFDD240 pKa = 6.38PISAEE245 pKa = 4.17LNLPTPAVVTQAKK258 pKa = 10.15EE259 pKa = 3.9DD260 pKa = 4.06TTKK263 pKa = 9.41HH264 pKa = 4.8TSNTEE269 pKa = 3.94EE270 pKa = 4.25EE271 pKa = 4.27DD272 pKa = 3.89GKK274 pKa = 11.08AKK276 pKa = 10.23PHH278 pKa = 6.01

Molecular weight: 30.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1983

120

982

495.8

54.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.413 ± 1.046

2.118 ± 0.337

5.093 ± 0.535

7.211 ± 0.991

2.471 ± 0.316

7.665 ± 1.002

1.261 ± 0.184

4.488 ± 0.453

6.001 ± 0.504

8.926 ± 0.734

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.614 ± 0.118

3.48 ± 0.39

5.547 ± 0.288

3.076 ± 0.319

5.547 ± 0.704

9.934 ± 1.093

5.497 ± 1.048

7.211 ± 0.503

2.118 ± 0.135

3.328 ± 0.533
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski