Pectobacterium phage MA12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9RH55|A0A6B9RH55_9CAUD Terminase large subunit OS=Pectobacterium phage MA12 OX=2686474 GN=MA12_gp29 PE=3 SV=1
MM1 pKa = 7.21KK2 pKa = 10.07QLTMSYY8 pKa = 8.81VQAVSFFTMGFTNDD22 pKa = 2.84ADD24 pKa = 4.04KK25 pKa = 11.45YY26 pKa = 8.24QTALSLHH33 pKa = 6.45GGGQIEE39 pKa = 4.51LLEE42 pKa = 4.51SVLPLAVALEE52 pKa = 4.1NVYY55 pKa = 10.74DD56 pKa = 5.47AIADD60 pKa = 3.59HH61 pKa = 7.27HH62 pKa = 7.26DD63 pKa = 3.58NCSVVWGYY71 pKa = 11.39DD72 pKa = 3.31VVEE75 pKa = 4.67MFGEE79 pKa = 4.73WVADD83 pKa = 4.71FMADD87 pKa = 3.5PDD89 pKa = 4.09NGGSVPDD96 pKa = 3.71QSAIGYY102 pKa = 7.23LIPMLSEE109 pKa = 4.22AFNVCNDD116 pKa = 2.7VDD118 pKa = 4.06RR119 pKa = 11.84FGDD122 pKa = 3.33IVGQAFRR129 pKa = 11.84DD130 pKa = 3.99GLKK133 pKa = 10.37EE134 pKa = 3.88VV135 pKa = 3.85

Molecular weight: 14.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9RP20|A0A6B9RP20_9CAUD Major capsid protein OS=Pectobacterium phage MA12 OX=2686474 GN=MA12_gp33 PE=3 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84LKK4 pKa = 11.01ASMATARR11 pKa = 11.84TSFRR15 pKa = 11.84ATPKK19 pKa = 9.75IASRR23 pKa = 11.84LNRR26 pKa = 11.84SAASIHH32 pKa = 6.06SFFEE36 pKa = 4.32FACSKK41 pKa = 10.93SGMISSATSAACSFSISRR59 pKa = 11.84ACFMNVARR67 pKa = 11.84VISEE71 pKa = 4.07SPSWWKK77 pKa = 10.15PFSHH81 pKa = 6.9LSSKK85 pKa = 10.96EE86 pKa = 4.03SMSKK90 pKa = 10.24LKK92 pKa = 10.62AWADD96 pKa = 3.41HH97 pKa = 6.45FRR99 pKa = 11.84ATSYY103 pKa = 11.69ANSS106 pKa = 3.54

Molecular weight: 11.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38

0

38

12365

86

1391

325.4

35.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.877 ± 0.89

1.084 ± 0.17

6.413 ± 0.29

5.265 ± 0.394

3.882 ± 0.242

7.279 ± 0.306

1.488 ± 0.179

5.208 ± 0.161

4.974 ± 0.385

7.837 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.822 ± 0.132

4.003 ± 0.145

4.092 ± 0.432

4.141 ± 0.453

5.815 ± 0.274

6.793 ± 0.3

6.227 ± 0.29

6.874 ± 0.373

1.731 ± 0.182

3.195 ± 0.252
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski