Amino acid dipepetide frequency for Pectobacterium phage MA12

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.83AlaAla: 13.83 ± 2.583
1.213AlaCys: 1.213 ± 0.401
6.228AlaAsp: 6.228 ± 0.929
5.581AlaGlu: 5.581 ± 0.939
3.882AlaPhe: 3.882 ± 0.511
8.169AlaGly: 8.169 ± 0.945
0.971AlaHis: 0.971 ± 0.327
5.823AlaIle: 5.823 ± 0.992
4.691AlaLys: 4.691 ± 1.285
8.25AlaLeu: 8.25 ± 1.056
2.831AlaMet: 2.831 ± 0.554
4.61AlaAsn: 4.61 ± 0.568
3.963AlaPro: 3.963 ± 0.64
5.419AlaGln: 5.419 ± 1.155
6.794AlaArg: 6.794 ± 0.797
8.25AlaSer: 8.25 ± 0.978
5.904AlaThr: 5.904 ± 1.103
8.25AlaVal: 8.25 ± 1.022
1.213AlaTrp: 1.213 ± 0.359
2.912AlaTyr: 2.912 ± 0.479
0.0AlaXaa: 0.0 ± 0.0
Cys
0.971CysAla: 0.971 ± 0.312
0.243CysCys: 0.243 ± 0.144
0.485CysAsp: 0.485 ± 0.243
0.404CysGlu: 0.404 ± 0.178
0.728CysPhe: 0.728 ± 0.277
0.89CysGly: 0.89 ± 0.31
0.081CysHis: 0.081 ± 0.082
0.566CysIle: 0.566 ± 0.2
0.324CysLys: 0.324 ± 0.136
0.971CysLeu: 0.971 ± 0.31
0.324CysMet: 0.324 ± 0.159
0.485CysAsn: 0.485 ± 0.2
0.89CysPro: 0.89 ± 0.318
0.324CysGln: 0.324 ± 0.172
0.728CysArg: 0.728 ± 0.252
0.728CysSer: 0.728 ± 0.333
0.162CysThr: 0.162 ± 0.113
0.647CysVal: 0.647 ± 0.282
0.485CysTrp: 0.485 ± 0.182
0.404CysTyr: 0.404 ± 0.217
0.0CysXaa: 0.0 ± 0.0
Asp
6.713AspAla: 6.713 ± 0.644
0.243AspCys: 0.243 ± 0.141
4.368AspAsp: 4.368 ± 0.83
3.154AspGlu: 3.154 ± 0.555
2.993AspPhe: 2.993 ± 0.63
5.419AspGly: 5.419 ± 0.855
1.132AspHis: 1.132 ± 0.349
3.235AspIle: 3.235 ± 0.617
2.588AspLys: 2.588 ± 0.469
5.338AspLeu: 5.338 ± 0.603
2.426AspMet: 2.426 ± 0.417
2.831AspAsn: 2.831 ± 0.345
2.669AspPro: 2.669 ± 0.81
2.022AspGln: 2.022 ± 0.366
2.912AspArg: 2.912 ± 0.691
3.559AspSer: 3.559 ± 0.661
3.559AspThr: 3.559 ± 0.471
5.095AspVal: 5.095 ± 0.663
1.132AspTrp: 1.132 ± 0.309
2.75AspTyr: 2.75 ± 0.605
0.0AspXaa: 0.0 ± 0.0
Glu
5.985GluAla: 5.985 ± 1.049
0.485GluCys: 0.485 ± 0.175
3.235GluAsp: 3.235 ± 0.491
3.64GluGlu: 3.64 ± 0.728
1.779GluPhe: 1.779 ± 0.348
2.669GluGly: 2.669 ± 0.484
0.404GluHis: 0.404 ± 0.222
3.154GluIle: 3.154 ± 0.56
2.103GluLys: 2.103 ± 0.546
5.176GluLeu: 5.176 ± 0.617
1.941GluMet: 1.941 ± 0.379
2.022GluAsn: 2.022 ± 0.423
1.132GluPro: 1.132 ± 0.346
2.103GluGln: 2.103 ± 0.646
3.559GluArg: 3.559 ± 0.581
3.559GluSer: 3.559 ± 0.507
3.882GluThr: 3.882 ± 0.463
3.478GluVal: 3.478 ± 0.568
1.294GluTrp: 1.294 ± 0.261
1.051GluTyr: 1.051 ± 0.269
0.0GluXaa: 0.0 ± 0.0
Phe
3.478PheAla: 3.478 ± 0.536
0.324PheCys: 0.324 ± 0.188
2.507PheAsp: 2.507 ± 0.541
2.022PheGlu: 2.022 ± 0.453
1.537PhePhe: 1.537 ± 0.31
2.993PheGly: 2.993 ± 0.553
0.728PheHis: 0.728 ± 0.218
2.831PheIle: 2.831 ± 0.451
1.294PheLys: 1.294 ± 0.291
2.184PheLeu: 2.184 ± 0.49
0.971PheMet: 0.971 ± 0.192
1.779PheAsn: 1.779 ± 0.376
1.537PhePro: 1.537 ± 0.335
1.375PheGln: 1.375 ± 0.276
2.588PheArg: 2.588 ± 0.346
3.235PheSer: 3.235 ± 0.477
2.831PheThr: 2.831 ± 0.541
2.103PheVal: 2.103 ± 0.34
0.971PheTrp: 0.971 ± 0.238
1.537PheTyr: 1.537 ± 0.38
0.0PheXaa: 0.0 ± 0.0
Gly
7.037GlyAla: 7.037 ± 0.824
0.971GlyCys: 0.971 ± 0.322
4.934GlyAsp: 4.934 ± 0.713
3.64GlyGlu: 3.64 ± 0.694
2.993GlyPhe: 2.993 ± 0.459
6.39GlyGly: 6.39 ± 0.836
0.728GlyHis: 0.728 ± 0.228
3.559GlyIle: 3.559 ± 0.523
4.206GlyLys: 4.206 ± 0.818
5.823GlyLeu: 5.823 ± 0.742
1.698GlyMet: 1.698 ± 0.352
2.265GlyAsn: 2.265 ± 0.338
1.698GlyPro: 1.698 ± 0.362
3.64GlyGln: 3.64 ± 0.416
4.691GlyArg: 4.691 ± 0.744
5.095GlySer: 5.095 ± 0.737
4.772GlyThr: 4.772 ± 0.703
4.61GlyVal: 4.61 ± 0.649
1.537GlyTrp: 1.537 ± 0.463
2.507GlyTyr: 2.507 ± 0.493
0.0GlyXaa: 0.0 ± 0.0
His
1.213HisAla: 1.213 ± 0.361
0.404HisCys: 0.404 ± 0.213
1.132HisAsp: 1.132 ± 0.359
0.404HisGlu: 0.404 ± 0.216
0.809HisPhe: 0.809 ± 0.267
1.779HisGly: 1.779 ± 0.367
0.566HisHis: 0.566 ± 0.231
0.89HisIle: 0.89 ± 0.344
0.809HisLys: 0.809 ± 0.287
1.294HisLeu: 1.294 ± 0.307
0.243HisMet: 0.243 ± 0.145
0.243HisAsn: 0.243 ± 0.182
0.647HisPro: 0.647 ± 0.24
0.324HisGln: 0.324 ± 0.149
0.971HisArg: 0.971 ± 0.33
0.728HisSer: 0.728 ± 0.244
0.728HisThr: 0.728 ± 0.286
0.89HisVal: 0.89 ± 0.247
0.324HisTrp: 0.324 ± 0.136
0.404HisTyr: 0.404 ± 0.191
0.0HisXaa: 0.0 ± 0.0
Ile
5.338IleAla: 5.338 ± 0.6
0.809IleCys: 0.809 ± 0.29
4.61IleAsp: 4.61 ± 0.522
2.588IleGlu: 2.588 ± 0.466
1.618IlePhe: 1.618 ± 0.363
4.044IleGly: 4.044 ± 0.503
0.728IleHis: 0.728 ± 0.253
2.022IleIle: 2.022 ± 0.288
2.912IleLys: 2.912 ± 0.643
2.912IleLeu: 2.912 ± 0.493
0.728IleMet: 0.728 ± 0.227
2.831IleAsn: 2.831 ± 0.289
2.184IlePro: 2.184 ± 0.473
1.779IleGln: 1.779 ± 0.517
3.316IleArg: 3.316 ± 0.503
4.206IleSer: 4.206 ± 0.676
3.073IleThr: 3.073 ± 0.518
3.559IleVal: 3.559 ± 0.52
0.485IleTrp: 0.485 ± 0.221
2.346IleTyr: 2.346 ± 0.498
0.0IleXaa: 0.0 ± 0.0
Lys
5.662LysAla: 5.662 ± 0.749
0.243LysCys: 0.243 ± 0.168
2.912LysAsp: 2.912 ± 0.623
2.831LysGlu: 2.831 ± 0.646
1.051LysPhe: 1.051 ± 0.215
2.831LysGly: 2.831 ± 0.592
0.728LysHis: 0.728 ± 0.267
2.669LysIle: 2.669 ± 0.44
3.882LysLys: 3.882 ± 0.849
4.287LysLeu: 4.287 ± 0.531
1.698LysMet: 1.698 ± 0.406
1.86LysAsn: 1.86 ± 0.333
2.588LysPro: 2.588 ± 0.526
1.618LysGln: 1.618 ± 0.528
3.397LysArg: 3.397 ± 0.808
2.75LysSer: 2.75 ± 0.405
3.64LysThr: 3.64 ± 0.724
2.346LysVal: 2.346 ± 0.364
1.132LysTrp: 1.132 ± 0.274
1.618LysTyr: 1.618 ± 0.346
0.0LysXaa: 0.0 ± 0.0
Leu
8.169LeuAla: 8.169 ± 0.86
0.647LeuCys: 0.647 ± 0.227
4.853LeuAsp: 4.853 ± 0.563
4.691LeuGlu: 4.691 ± 0.636
2.831LeuPhe: 2.831 ± 0.548
4.853LeuGly: 4.853 ± 0.621
1.213LeuHis: 1.213 ± 0.39
3.478LeuIle: 3.478 ± 0.445
5.095LeuLys: 5.095 ± 0.838
5.257LeuLeu: 5.257 ± 0.577
2.426LeuMet: 2.426 ± 0.36
3.72LeuAsn: 3.72 ± 0.684
3.073LeuPro: 3.073 ± 0.585
3.559LeuGln: 3.559 ± 0.809
3.478LeuArg: 3.478 ± 0.487
5.742LeuSer: 5.742 ± 0.724
5.823LeuThr: 5.823 ± 0.576
5.176LeuVal: 5.176 ± 0.504
1.213LeuTrp: 1.213 ± 0.345
3.073LeuTyr: 3.073 ± 0.619
0.0LeuXaa: 0.0 ± 0.0
Met
4.853MetAla: 4.853 ± 0.72
0.485MetCys: 0.485 ± 0.21
1.698MetAsp: 1.698 ± 0.454
0.566MetGlu: 0.566 ± 0.192
0.809MetPhe: 0.809 ± 0.271
1.618MetGly: 1.618 ± 0.337
0.728MetHis: 0.728 ± 0.265
1.537MetIle: 1.537 ± 0.34
1.779MetLys: 1.779 ± 0.35
1.537MetLeu: 1.537 ± 0.385
1.051MetMet: 1.051 ± 0.285
0.809MetAsn: 0.809 ± 0.233
1.456MetPro: 1.456 ± 0.309
1.294MetGln: 1.294 ± 0.273
1.132MetArg: 1.132 ± 0.352
2.75MetSer: 2.75 ± 0.363
1.456MetThr: 1.456 ± 0.427
1.86MetVal: 1.86 ± 0.402
0.243MetTrp: 0.243 ± 0.272
0.566MetTyr: 0.566 ± 0.289
0.0MetXaa: 0.0 ± 0.0
Asn
4.853AsnAla: 4.853 ± 0.608
0.243AsnCys: 0.243 ± 0.126
2.75AsnAsp: 2.75 ± 0.46
1.618AsnGlu: 1.618 ± 0.399
1.537AsnPhe: 1.537 ± 0.248
3.316AsnGly: 3.316 ± 0.419
0.404AsnHis: 0.404 ± 0.177
1.779AsnIle: 1.779 ± 0.349
1.537AsnLys: 1.537 ± 0.49
3.073AsnLeu: 3.073 ± 0.427
0.89AsnMet: 0.89 ± 0.296
1.86AsnAsn: 1.86 ± 0.321
2.507AsnPro: 2.507 ± 0.458
1.456AsnGln: 1.456 ± 0.558
2.346AsnArg: 2.346 ± 0.378
2.588AsnSer: 2.588 ± 0.404
2.75AsnThr: 2.75 ± 0.372
2.75AsnVal: 2.75 ± 0.431
0.809AsnTrp: 0.809 ± 0.237
0.971AsnTyr: 0.971 ± 0.29
0.0AsnXaa: 0.0 ± 0.0
Pro
4.044ProAla: 4.044 ± 0.819
0.404ProCys: 0.404 ± 0.193
2.831ProAsp: 2.831 ± 0.552
2.103ProGlu: 2.103 ± 0.536
2.022ProPhe: 2.022 ± 0.406
3.64ProGly: 3.64 ± 0.43
0.971ProHis: 0.971 ± 0.24
2.022ProIle: 2.022 ± 0.512
1.698ProLys: 1.698 ± 0.317
1.779ProLeu: 1.779 ± 0.38
1.132ProMet: 1.132 ± 0.34
2.022ProAsn: 2.022 ± 0.399
1.537ProPro: 1.537 ± 0.401
1.456ProGln: 1.456 ± 0.304
1.779ProArg: 1.779 ± 0.428
3.397ProSer: 3.397 ± 0.617
2.588ProThr: 2.588 ± 0.68
2.912ProVal: 2.912 ± 0.452
0.89ProTrp: 0.89 ± 0.25
1.698ProTyr: 1.698 ± 0.409
0.0ProXaa: 0.0 ± 0.0
Gln
4.61GlnAla: 4.61 ± 1.051
0.728GlnCys: 0.728 ± 0.31
2.022GlnAsp: 2.022 ± 0.387
2.265GlnGlu: 2.265 ± 0.444
1.618GlnPhe: 1.618 ± 0.421
2.831GlnGly: 2.831 ± 0.411
0.647GlnHis: 0.647 ± 0.254
1.86GlnIle: 1.86 ± 0.297
2.265GlnLys: 2.265 ± 0.574
3.64GlnLeu: 3.64 ± 0.749
1.537GlnMet: 1.537 ± 0.404
1.213GlnAsn: 1.213 ± 0.252
1.941GlnPro: 1.941 ± 0.294
2.426GlnGln: 2.426 ± 0.829
2.831GlnArg: 2.831 ± 0.423
1.86GlnSer: 1.86 ± 0.594
2.831GlnThr: 2.831 ± 0.501
2.184GlnVal: 2.184 ± 0.416
0.566GlnTrp: 0.566 ± 0.189
1.537GlnTyr: 1.537 ± 0.315
0.0GlnXaa: 0.0 ± 0.0
Arg
5.095ArgAla: 5.095 ± 0.72
0.809ArgCys: 0.809 ± 0.253
4.044ArgAsp: 4.044 ± 0.535
3.559ArgGlu: 3.559 ± 0.55
2.669ArgPhe: 2.669 ± 0.48
3.559ArgGly: 3.559 ± 0.691
1.213ArgHis: 1.213 ± 0.33
3.397ArgIle: 3.397 ± 0.479
2.184ArgLys: 2.184 ± 0.387
6.228ArgLeu: 6.228 ± 0.705
1.941ArgMet: 1.941 ± 0.433
2.265ArgAsn: 2.265 ± 0.38
2.265ArgPro: 2.265 ± 0.466
3.235ArgGln: 3.235 ± 0.795
4.368ArgArg: 4.368 ± 0.617
2.588ArgSer: 2.588 ± 0.433
2.831ArgThr: 2.831 ± 0.643
4.044ArgVal: 4.044 ± 0.509
0.566ArgTrp: 0.566 ± 0.239
1.294ArgTyr: 1.294 ± 0.263
0.0ArgXaa: 0.0 ± 0.0
Ser
7.522SerAla: 7.522 ± 0.963
0.809SerCys: 0.809 ± 0.326
3.882SerAsp: 3.882 ± 0.63
3.64SerGlu: 3.64 ± 0.574
3.64SerPhe: 3.64 ± 0.585
5.823SerGly: 5.823 ± 0.647
1.213SerHis: 1.213 ± 0.343
4.368SerIle: 4.368 ± 0.495
2.75SerLys: 2.75 ± 0.568
6.39SerLeu: 6.39 ± 0.723
1.375SerMet: 1.375 ± 0.442
1.618SerAsn: 1.618 ± 0.31
1.86SerPro: 1.86 ± 0.393
2.103SerGln: 2.103 ± 0.434
3.316SerArg: 3.316 ± 0.583
4.125SerSer: 4.125 ± 0.687
3.72SerThr: 3.72 ± 0.482
5.015SerVal: 5.015 ± 0.805
1.375SerTrp: 1.375 ± 0.36
3.397SerTyr: 3.397 ± 0.504
0.0SerXaa: 0.0 ± 0.0
Thr
6.956ThrAla: 6.956 ± 1.551
0.485ThrCys: 0.485 ± 0.219
2.831ThrAsp: 2.831 ± 0.425
2.912ThrGlu: 2.912 ± 0.473
2.993ThrPhe: 2.993 ± 0.495
4.61ThrGly: 4.61 ± 0.528
0.485ThrHis: 0.485 ± 0.226
2.912ThrIle: 2.912 ± 0.369
3.559ThrLys: 3.559 ± 0.42
5.338ThrLeu: 5.338 ± 0.781
1.375ThrMet: 1.375 ± 0.281
2.426ThrAsn: 2.426 ± 0.452
3.559ThrPro: 3.559 ± 0.548
2.103ThrGln: 2.103 ± 0.387
3.316ThrArg: 3.316 ± 0.643
4.368ThrSer: 4.368 ± 0.788
4.448ThrThr: 4.448 ± 0.746
5.095ThrVal: 5.095 ± 0.619
1.132ThrTrp: 1.132 ± 0.235
1.375ThrTyr: 1.375 ± 0.262
0.0ThrXaa: 0.0 ± 0.0
Val
8.088ValAla: 8.088 ± 0.869
0.809ValCys: 0.809 ± 0.268
4.853ValAsp: 4.853 ± 0.433
4.368ValGlu: 4.368 ± 0.483
1.941ValPhe: 1.941 ± 0.512
4.368ValGly: 4.368 ± 0.743
0.971ValHis: 0.971 ± 0.35
3.316ValIle: 3.316 ± 0.481
3.478ValLys: 3.478 ± 0.504
4.934ValLeu: 4.934 ± 0.614
2.184ValMet: 2.184 ± 0.442
2.588ValAsn: 2.588 ± 0.409
2.507ValPro: 2.507 ± 0.477
3.316ValGln: 3.316 ± 0.626
3.963ValArg: 3.963 ± 0.639
5.095ValSer: 5.095 ± 0.69
4.853ValThr: 4.853 ± 0.818
4.853ValVal: 4.853 ± 1.059
0.809ValTrp: 0.809 ± 0.236
1.456ValTyr: 1.456 ± 0.311
0.0ValXaa: 0.0 ± 0.0
Trp
1.86TrpAla: 1.86 ± 0.551
0.162TrpCys: 0.162 ± 0.132
1.132TrpAsp: 1.132 ± 0.32
0.809TrpGlu: 0.809 ± 0.283
0.485TrpPhe: 0.485 ± 0.221
0.647TrpGly: 0.647 ± 0.179
0.324TrpHis: 0.324 ± 0.16
1.132TrpIle: 1.132 ± 0.264
1.132TrpLys: 1.132 ± 0.387
1.698TrpLeu: 1.698 ± 0.475
0.647TrpMet: 0.647 ± 0.246
0.728TrpAsn: 0.728 ± 0.213
1.537TrpPro: 1.537 ± 0.361
0.485TrpGln: 0.485 ± 0.191
0.728TrpArg: 0.728 ± 0.273
0.809TrpSer: 0.809 ± 0.257
0.971TrpThr: 0.971 ± 0.294
1.213TrpVal: 1.213 ± 0.289
0.485TrpTrp: 0.485 ± 0.221
0.324TrpTyr: 0.324 ± 0.145
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.507TyrAla: 2.507 ± 0.404
0.324TyrCys: 0.324 ± 0.215
2.831TyrAsp: 2.831 ± 0.46
1.86TyrGlu: 1.86 ± 0.408
0.89TyrPhe: 0.89 ± 0.233
2.346TyrGly: 2.346 ± 0.659
0.647TyrHis: 0.647 ± 0.225
1.537TyrIle: 1.537 ± 0.377
1.456TyrLys: 1.456 ± 0.344
2.265TyrLeu: 2.265 ± 0.567
0.728TyrMet: 0.728 ± 0.262
1.86TyrAsn: 1.86 ± 0.325
1.375TyrPro: 1.375 ± 0.343
1.375TyrGln: 1.375 ± 0.497
1.941TyrArg: 1.941 ± 0.369
2.507TyrSer: 2.507 ± 0.428
1.456TyrThr: 1.456 ± 0.304
2.669TyrVal: 2.669 ± 0.896
0.647TyrTrp: 0.647 ± 0.209
0.728TyrTyr: 0.728 ± 0.232
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 38 proteins (12365 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski