Bacillus phage BSP7

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5H2QBM5|A0A5H2QBM5_9VIRU Putative QueE-like protein OS=Bacillus phage BSP7 OX=2301661 GN=BSP7_051 PE=3 SV=1
MM1 pKa = 6.03EE2 pKa = 4.42TEE4 pKa = 4.11YY5 pKa = 11.03FGLNVSTYY13 pKa = 10.52KK14 pKa = 10.36SVYY17 pKa = 10.4DD18 pKa = 3.63SGQSVIIDD26 pKa = 3.41IEE28 pKa = 4.01NWSDD32 pKa = 2.93VDD34 pKa = 3.76KK35 pKa = 11.17VVHH38 pKa = 6.08VKK40 pKa = 10.56PFMITEE46 pKa = 4.31DD47 pKa = 3.76ARR49 pKa = 11.84LVDD52 pKa = 3.71PDD54 pKa = 4.17VYY56 pKa = 10.84PFNLQVLAGSKK67 pKa = 10.05DD68 pKa = 3.41QAIFTTGDD76 pKa = 3.25GGDD79 pKa = 3.37IPTPGTYY86 pKa = 9.72YY87 pKa = 10.8FKK89 pKa = 11.37LEE91 pKa = 4.03VEE93 pKa = 4.48GEE95 pKa = 4.39GEE97 pKa = 4.24FFFEE101 pKa = 4.0QGIEE105 pKa = 3.94IGLKK109 pKa = 9.93

Molecular weight: 12.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5H2QDC9|A0A5H2QDC9_9VIRU Uncharacterized protein OS=Bacillus phage BSP7 OX=2301661 GN=BSP7_033 PE=4 SV=1
MM1 pKa = 7.5PRR3 pKa = 11.84SINVGSRR10 pKa = 11.84LNNYY14 pKa = 7.91FNRR17 pKa = 11.84KK18 pKa = 8.17IAGLYY23 pKa = 10.59GLAQNAAQQMEE34 pKa = 4.38GQAKK38 pKa = 9.73RR39 pKa = 11.84KK40 pKa = 9.79ARR42 pKa = 11.84WTDD45 pKa = 2.91RR46 pKa = 11.84TGNARR51 pKa = 11.84QGLKK55 pKa = 10.7GKK57 pKa = 8.08VVKK60 pKa = 8.98TNNMITIYY68 pKa = 10.18LIHH71 pKa = 6.37SVSYY75 pKa = 10.59GIYY78 pKa = 10.05LEE80 pKa = 4.16KK81 pKa = 10.8ARR83 pKa = 11.84SGKK86 pKa = 9.65YY87 pKa = 10.43AIIKK91 pKa = 9.38PIFRR95 pKa = 11.84ANQSNVMSSLRR106 pKa = 11.84RR107 pKa = 11.84YY108 pKa = 8.61WDD110 pKa = 3.17NTT112 pKa = 3.42

Molecular weight: 12.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

17113

30

1120

244.5

27.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.269 ± 0.395

0.818 ± 0.112

6.247 ± 0.26

7.462 ± 0.357

3.915 ± 0.134

6.562 ± 0.325

1.876 ± 0.151

6.364 ± 0.232

7.789 ± 0.34

7.392 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.969 ± 0.167

5.218 ± 0.211

3.652 ± 0.175

3.576 ± 0.161

4.646 ± 0.201

5.937 ± 0.26

6.217 ± 0.215

6.416 ± 0.207

1.584 ± 0.139

4.09 ± 0.265
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski