Amino acid dipepetide frequency for Bacillus phage BSP7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.552AlaAla: 5.552 ± 0.838
0.526AlaCys: 0.526 ± 0.221
5.201AlaAsp: 5.201 ± 0.683
5.084AlaGlu: 5.084 ± 0.582
2.747AlaPhe: 2.747 ± 0.442
5.259AlaGly: 5.259 ± 0.622
0.935AlaHis: 0.935 ± 0.222
4.5AlaIle: 4.5 ± 0.521
6.37AlaLys: 6.37 ± 0.896
5.435AlaLeu: 5.435 ± 0.557
2.221AlaMet: 2.221 ± 0.334
3.448AlaAsn: 3.448 ± 0.571
1.987AlaPro: 1.987 ± 0.37
2.922AlaGln: 2.922 ± 0.446
4.266AlaArg: 4.266 ± 0.604
3.799AlaSer: 3.799 ± 0.403
4.091AlaThr: 4.091 ± 0.573
4.149AlaVal: 4.149 ± 0.528
1.169AlaTrp: 1.169 ± 0.255
3.039AlaTyr: 3.039 ± 0.425
0.0AlaXaa: 0.0 ± 0.0
Cys
0.526CysAla: 0.526 ± 0.178
0.117CysCys: 0.117 ± 0.082
1.052CysAsp: 1.052 ± 0.227
0.409CysGlu: 0.409 ± 0.135
0.234CysPhe: 0.234 ± 0.111
0.877CysGly: 0.877 ± 0.268
0.292CysHis: 0.292 ± 0.129
0.468CysIle: 0.468 ± 0.177
0.526CysLys: 0.526 ± 0.197
0.526CysLeu: 0.526 ± 0.15
0.292CysMet: 0.292 ± 0.122
0.175CysAsn: 0.175 ± 0.105
0.468CysPro: 0.468 ± 0.167
0.409CysGln: 0.409 ± 0.157
0.292CysArg: 0.292 ± 0.12
0.175CysSer: 0.175 ± 0.086
0.526CysThr: 0.526 ± 0.202
0.292CysVal: 0.292 ± 0.126
0.117CysTrp: 0.117 ± 0.083
0.409CysTyr: 0.409 ± 0.14
0.0CysXaa: 0.0 ± 0.0
Asp
3.74AspAla: 3.74 ± 0.427
0.643AspCys: 0.643 ± 0.197
4.441AspAsp: 4.441 ± 0.726
4.208AspGlu: 4.208 ± 0.545
2.98AspPhe: 2.98 ± 0.549
4.383AspGly: 4.383 ± 0.635
0.877AspHis: 0.877 ± 0.287
5.61AspIle: 5.61 ± 0.645
3.448AspLys: 3.448 ± 0.451
4.617AspLeu: 4.617 ± 0.609
2.045AspMet: 2.045 ± 0.367
2.396AspAsn: 2.396 ± 0.374
2.63AspPro: 2.63 ± 0.457
1.928AspGln: 1.928 ± 0.334
2.98AspArg: 2.98 ± 0.442
4.032AspSer: 4.032 ± 0.516
3.623AspThr: 3.623 ± 0.416
3.682AspVal: 3.682 ± 0.387
1.286AspTrp: 1.286 ± 0.299
2.922AspTyr: 2.922 ± 0.381
0.0AspXaa: 0.0 ± 0.0
Glu
6.72GluAla: 6.72 ± 0.956
0.292GluCys: 0.292 ± 0.132
4.149GluAsp: 4.149 ± 0.423
5.785GluGlu: 5.785 ± 0.865
3.097GluPhe: 3.097 ± 0.46
4.967GluGly: 4.967 ± 0.653
1.695GluHis: 1.695 ± 0.338
4.383GluIle: 4.383 ± 0.46
5.201GluLys: 5.201 ± 0.537
5.785GluLeu: 5.785 ± 0.622
2.396GluMet: 2.396 ± 0.47
3.974GluAsn: 3.974 ± 0.476
2.63GluPro: 2.63 ± 0.584
3.273GluGln: 3.273 ± 0.395
3.039GluArg: 3.039 ± 0.405
3.682GluSer: 3.682 ± 0.444
3.74GluThr: 3.74 ± 0.493
5.493GluVal: 5.493 ± 0.533
1.519GluTrp: 1.519 ± 0.256
2.805GluTyr: 2.805 ± 0.486
0.0GluXaa: 0.0 ± 0.0
Phe
1.928PheAla: 1.928 ± 0.324
0.175PheCys: 0.175 ± 0.103
2.571PheAsp: 2.571 ± 0.417
3.915PheGlu: 3.915 ± 0.664
1.578PhePhe: 1.578 ± 0.328
2.454PheGly: 2.454 ± 0.335
0.76PheHis: 0.76 ± 0.204
2.162PheIle: 2.162 ± 0.4
3.565PheLys: 3.565 ± 0.466
2.805PheLeu: 2.805 ± 0.432
0.993PheMet: 0.993 ± 0.255
2.688PheAsn: 2.688 ± 0.352
1.169PhePro: 1.169 ± 0.327
1.11PheGln: 1.11 ± 0.223
1.636PheArg: 1.636 ± 0.239
2.162PheSer: 2.162 ± 0.334
2.571PheThr: 2.571 ± 0.389
2.805PheVal: 2.805 ± 0.529
0.175PheTrp: 0.175 ± 0.093
1.928PheTyr: 1.928 ± 0.306
0.0PheXaa: 0.0 ± 0.0
Gly
4.032GlyAla: 4.032 ± 0.484
0.468GlyCys: 0.468 ± 0.189
3.974GlyAsp: 3.974 ± 0.41
4.967GlyGlu: 4.967 ± 0.668
3.156GlyPhe: 3.156 ± 0.454
3.915GlyGly: 3.915 ± 0.628
1.344GlyHis: 1.344 ± 0.284
5.259GlyIle: 5.259 ± 0.658
5.493GlyLys: 5.493 ± 0.539
4.5GlyLeu: 4.5 ± 0.542
2.045GlyMet: 2.045 ± 0.393
3.039GlyAsn: 3.039 ± 0.564
1.344GlyPro: 1.344 ± 0.306
1.928GlyGln: 1.928 ± 0.314
2.922GlyArg: 2.922 ± 0.457
3.799GlySer: 3.799 ± 0.618
5.143GlyThr: 5.143 ± 0.695
3.214GlyVal: 3.214 ± 0.395
1.578GlyTrp: 1.578 ± 0.353
3.506GlyTyr: 3.506 ± 0.468
0.0GlyXaa: 0.0 ± 0.0
His
1.461HisAla: 1.461 ± 0.301
0.409HisCys: 0.409 ± 0.156
1.227HisAsp: 1.227 ± 0.262
1.227HisGlu: 1.227 ± 0.243
0.701HisPhe: 0.701 ± 0.195
1.169HisGly: 1.169 ± 0.329
0.584HisHis: 0.584 ± 0.204
1.461HisIle: 1.461 ± 0.36
1.286HisLys: 1.286 ± 0.258
1.403HisLeu: 1.403 ± 0.383
0.409HisMet: 0.409 ± 0.166
0.993HisAsn: 0.993 ± 0.186
0.76HisPro: 0.76 ± 0.271
0.526HisGln: 0.526 ± 0.185
0.701HisArg: 0.701 ± 0.218
0.818HisSer: 0.818 ± 0.19
1.052HisThr: 1.052 ± 0.271
1.169HisVal: 1.169 ± 0.226
0.468HisTrp: 0.468 ± 0.155
0.935HisTyr: 0.935 ± 0.212
0.0HisXaa: 0.0 ± 0.0
Ile
5.201IleAla: 5.201 ± 0.528
0.351IleCys: 0.351 ± 0.122
4.734IleAsp: 4.734 ± 0.481
5.201IleGlu: 5.201 ± 0.681
2.396IlePhe: 2.396 ± 0.386
3.273IleGly: 3.273 ± 0.552
1.286IleHis: 1.286 ± 0.344
3.623IleIle: 3.623 ± 0.636
5.376IleLys: 5.376 ± 0.561
4.266IleLeu: 4.266 ± 0.472
1.344IleMet: 1.344 ± 0.231
3.389IleAsn: 3.389 ± 0.437
2.98IlePro: 2.98 ± 0.339
2.338IleGln: 2.338 ± 0.388
3.331IleArg: 3.331 ± 0.455
2.922IleSer: 2.922 ± 0.345
4.5IleThr: 4.5 ± 0.638
3.974IleVal: 3.974 ± 0.394
0.935IleTrp: 0.935 ± 0.221
2.221IleTyr: 2.221 ± 0.333
0.0IleXaa: 0.0 ± 0.0
Lys
5.727LysAla: 5.727 ± 0.574
0.468LysCys: 0.468 ± 0.145
5.201LysAsp: 5.201 ± 0.536
6.019LysGlu: 6.019 ± 0.721
2.863LysPhe: 2.863 ± 0.438
4.675LysGly: 4.675 ± 0.524
1.812LysHis: 1.812 ± 0.333
4.5LysIle: 4.5 ± 0.387
6.253LysLys: 6.253 ± 0.924
5.727LysLeu: 5.727 ± 0.56
2.863LysMet: 2.863 ± 0.403
3.448LysAsn: 3.448 ± 0.463
3.389LysPro: 3.389 ± 0.497
3.623LysGln: 3.623 ± 0.412
2.863LysArg: 2.863 ± 0.305
4.266LysSer: 4.266 ± 0.464
4.85LysThr: 4.85 ± 0.679
5.902LysVal: 5.902 ± 0.466
1.052LysTrp: 1.052 ± 0.255
2.338LysTyr: 2.338 ± 0.376
0.0LysXaa: 0.0 ± 0.0
Leu
6.37LeuAla: 6.37 ± 0.702
0.701LeuCys: 0.701 ± 0.181
3.974LeuAsp: 3.974 ± 0.506
5.902LeuGlu: 5.902 ± 0.616
2.863LeuPhe: 2.863 ± 0.344
4.266LeuGly: 4.266 ± 0.44
1.403LeuHis: 1.403 ± 0.236
3.799LeuIle: 3.799 ± 0.493
5.61LeuLys: 5.61 ± 0.506
4.792LeuLeu: 4.792 ± 0.634
1.812LeuMet: 1.812 ± 0.336
4.85LeuAsn: 4.85 ± 0.744
3.389LeuPro: 3.389 ± 0.439
2.863LeuGln: 2.863 ± 0.468
3.623LeuArg: 3.623 ± 0.528
5.259LeuSer: 5.259 ± 0.598
4.909LeuThr: 4.909 ± 0.548
3.506LeuVal: 3.506 ± 0.563
1.403LeuTrp: 1.403 ± 0.269
2.63LeuTyr: 2.63 ± 0.347
0.0LeuXaa: 0.0 ± 0.0
Met
2.571MetAla: 2.571 ± 0.532
0.175MetCys: 0.175 ± 0.088
1.636MetAsp: 1.636 ± 0.288
2.747MetGlu: 2.747 ± 0.433
0.584MetPhe: 0.584 ± 0.165
1.286MetGly: 1.286 ± 0.279
0.993MetHis: 0.993 ± 0.306
1.987MetIle: 1.987 ± 0.371
1.987MetLys: 1.987 ± 0.351
2.454MetLeu: 2.454 ± 0.356
0.76MetMet: 0.76 ± 0.213
1.403MetAsn: 1.403 ± 0.243
0.935MetPro: 0.935 ± 0.222
1.169MetGln: 1.169 ± 0.211
1.344MetArg: 1.344 ± 0.3
2.279MetSer: 2.279 ± 0.295
2.162MetThr: 2.162 ± 0.352
1.987MetVal: 1.987 ± 0.407
0.351MetTrp: 0.351 ± 0.123
0.877MetTyr: 0.877 ± 0.224
0.0MetXaa: 0.0 ± 0.0
Asn
3.273AsnAla: 3.273 ± 0.445
0.292AsnCys: 0.292 ± 0.138
2.805AsnAsp: 2.805 ± 0.427
3.857AsnGlu: 3.857 ± 0.466
1.987AsnPhe: 1.987 ± 0.298
4.149AsnGly: 4.149 ± 0.781
0.877AsnHis: 0.877 ± 0.197
2.338AsnIle: 2.338 ± 0.384
3.974AsnLys: 3.974 ± 0.495
3.623AsnLeu: 3.623 ± 0.506
1.578AsnMet: 1.578 ± 0.325
3.682AsnAsn: 3.682 ± 0.483
2.571AsnPro: 2.571 ± 0.405
1.987AsnGln: 1.987 ± 0.295
2.396AsnArg: 2.396 ± 0.344
3.273AsnSer: 3.273 ± 0.515
3.097AsnThr: 3.097 ± 0.471
3.448AsnVal: 3.448 ± 0.413
0.409AsnTrp: 0.409 ± 0.162
2.571AsnTyr: 2.571 ± 0.347
0.0AsnXaa: 0.0 ± 0.0
Pro
2.162ProAla: 2.162 ± 0.357
0.058ProCys: 0.058 ± 0.066
2.338ProAsp: 2.338 ± 0.402
2.922ProGlu: 2.922 ± 0.391
2.162ProPhe: 2.162 ± 0.367
2.396ProGly: 2.396 ± 0.341
0.584ProHis: 0.584 ± 0.172
2.805ProIle: 2.805 ± 0.497
3.097ProLys: 3.097 ± 0.482
2.221ProLeu: 2.221 ± 0.353
0.935ProMet: 0.935 ± 0.281
1.812ProAsn: 1.812 ± 0.354
1.169ProPro: 1.169 ± 0.28
1.519ProGln: 1.519 ± 0.299
1.227ProArg: 1.227 ± 0.242
2.338ProSer: 2.338 ± 0.362
2.513ProThr: 2.513 ± 0.312
2.279ProVal: 2.279 ± 0.313
0.526ProTrp: 0.526 ± 0.18
1.461ProTyr: 1.461 ± 0.268
0.0ProXaa: 0.0 ± 0.0
Gln
3.039GlnAla: 3.039 ± 0.392
0.292GlnCys: 0.292 ± 0.13
1.636GlnAsp: 1.636 ± 0.257
3.331GlnGlu: 3.331 ± 0.543
1.052GlnPhe: 1.052 ± 0.254
2.454GlnGly: 2.454 ± 0.413
0.409GlnHis: 0.409 ± 0.165
2.454GlnIle: 2.454 ± 0.321
2.571GlnLys: 2.571 ± 0.347
3.214GlnLeu: 3.214 ± 0.458
1.227GlnMet: 1.227 ± 0.261
1.812GlnAsn: 1.812 ± 0.299
0.877GlnPro: 0.877 ± 0.2
1.461GlnGln: 1.461 ± 0.385
1.987GlnArg: 1.987 ± 0.335
2.513GlnSer: 2.513 ± 0.404
1.87GlnThr: 1.87 ± 0.327
2.162GlnVal: 2.162 ± 0.316
0.584GlnTrp: 0.584 ± 0.184
0.818GlnTyr: 0.818 ± 0.277
0.0GlnXaa: 0.0 ± 0.0
Arg
2.922ArgAla: 2.922 ± 0.472
0.292ArgCys: 0.292 ± 0.118
2.863ArgAsp: 2.863 ± 0.358
3.097ArgGlu: 3.097 ± 0.478
1.87ArgPhe: 1.87 ± 0.325
3.156ArgGly: 3.156 ± 0.486
0.643ArgHis: 0.643 ± 0.192
2.571ArgIle: 2.571 ± 0.303
3.915ArgLys: 3.915 ± 0.615
4.324ArgLeu: 4.324 ± 0.649
1.519ArgMet: 1.519 ± 0.308
2.396ArgAsn: 2.396 ± 0.341
1.519ArgPro: 1.519 ± 0.261
1.403ArgGln: 1.403 ± 0.323
2.571ArgArg: 2.571 ± 0.399
2.63ArgSer: 2.63 ± 0.303
2.747ArgThr: 2.747 ± 0.307
3.273ArgVal: 3.273 ± 0.411
1.169ArgTrp: 1.169 ± 0.271
1.578ArgTyr: 1.578 ± 0.254
0.0ArgXaa: 0.0 ± 0.0
Ser
5.143SerAla: 5.143 ± 0.585
0.468SerCys: 0.468 ± 0.201
3.039SerAsp: 3.039 ± 0.396
3.039SerGlu: 3.039 ± 0.459
2.221SerPhe: 2.221 ± 0.372
4.85SerGly: 4.85 ± 0.744
0.76SerHis: 0.76 ± 0.199
4.149SerIle: 4.149 ± 0.429
4.5SerLys: 4.5 ± 0.513
4.734SerLeu: 4.734 ± 0.509
1.227SerMet: 1.227 ± 0.228
3.389SerAsn: 3.389 ± 0.427
1.636SerPro: 1.636 ± 0.312
1.812SerGln: 1.812 ± 0.332
2.98SerArg: 2.98 ± 0.418
4.324SerSer: 4.324 ± 0.599
3.565SerThr: 3.565 ± 0.36
3.799SerVal: 3.799 ± 0.506
1.052SerTrp: 1.052 ± 0.243
2.688SerTyr: 2.688 ± 0.365
0.0SerXaa: 0.0 ± 0.0
Thr
5.026ThrAla: 5.026 ± 0.78
0.584ThrCys: 0.584 ± 0.22
4.324ThrAsp: 4.324 ± 0.554
4.5ThrGlu: 4.5 ± 0.492
2.396ThrPhe: 2.396 ± 0.399
5.435ThrGly: 5.435 ± 0.597
0.877ThrHis: 0.877 ± 0.232
4.208ThrIle: 4.208 ± 0.484
4.383ThrLys: 4.383 ± 0.49
5.026ThrLeu: 5.026 ± 0.531
2.045ThrMet: 2.045 ± 0.323
3.331ThrAsn: 3.331 ± 0.426
2.513ThrPro: 2.513 ± 0.362
1.578ThrGln: 1.578 ± 0.308
2.396ThrArg: 2.396 ± 0.375
2.747ThrSer: 2.747 ± 0.385
3.682ThrThr: 3.682 ± 0.551
4.149ThrVal: 4.149 ± 0.556
0.468ThrTrp: 0.468 ± 0.135
2.513ThrTyr: 2.513 ± 0.391
0.0ThrXaa: 0.0 ± 0.0
Val
4.032ValAla: 4.032 ± 0.397
0.701ValCys: 0.701 ± 0.241
3.623ValAsp: 3.623 ± 0.386
4.675ValGlu: 4.675 ± 0.46
1.928ValPhe: 1.928 ± 0.322
2.922ValGly: 2.922 ± 0.472
1.403ValHis: 1.403 ± 0.383
4.5ValIle: 4.5 ± 0.524
5.669ValLys: 5.669 ± 0.696
4.149ValLeu: 4.149 ± 0.527
2.104ValMet: 2.104 ± 0.424
3.214ValAsn: 3.214 ± 0.405
2.571ValPro: 2.571 ± 0.403
1.695ValGln: 1.695 ± 0.297
3.565ValArg: 3.565 ± 0.51
4.324ValSer: 4.324 ± 0.613
3.915ValThr: 3.915 ± 0.51
5.318ValVal: 5.318 ± 0.432
0.701ValTrp: 0.701 ± 0.179
3.156ValTyr: 3.156 ± 0.52
0.0ValXaa: 0.0 ± 0.0
Trp
0.993TrpAla: 0.993 ± 0.234
0.234TrpCys: 0.234 ± 0.113
1.11TrpAsp: 1.11 ± 0.24
0.877TrpGlu: 0.877 ± 0.211
0.526TrpPhe: 0.526 ± 0.198
1.11TrpGly: 1.11 ± 0.236
0.526TrpHis: 0.526 ± 0.198
0.526TrpIle: 0.526 ± 0.159
1.461TrpLys: 1.461 ± 0.305
1.461TrpLeu: 1.461 ± 0.276
0.76TrpMet: 0.76 ± 0.282
0.584TrpAsn: 0.584 ± 0.151
0.351TrpPro: 0.351 ± 0.154
0.818TrpGln: 0.818 ± 0.17
0.468TrpArg: 0.468 ± 0.204
1.11TrpSer: 1.11 ± 0.292
0.877TrpThr: 0.877 ± 0.215
1.052TrpVal: 1.052 ± 0.228
0.234TrpTrp: 0.234 ± 0.111
0.76TrpTyr: 0.76 ± 0.201
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.279TyrAla: 2.279 ± 0.357
0.935TyrCys: 0.935 ± 0.227
2.571TyrAsp: 2.571 ± 0.47
2.863TyrGlu: 2.863 ± 0.385
1.812TyrPhe: 1.812 ± 0.361
2.63TyrGly: 2.63 ± 0.355
0.701TyrHis: 0.701 ± 0.191
2.338TyrIle: 2.338 ± 0.305
3.214TyrLys: 3.214 ± 0.444
2.863TyrLeu: 2.863 ± 0.379
1.052TyrMet: 1.052 ± 0.213
2.162TyrAsn: 2.162 ± 0.479
1.636TyrPro: 1.636 ± 0.27
1.403TyrGln: 1.403 ± 0.358
1.87TyrArg: 1.87 ± 0.301
2.922TyrSer: 2.922 ± 0.624
2.747TyrThr: 2.747 ± 0.463
2.513TyrVal: 2.513 ± 0.368
0.643TyrTrp: 0.643 ± 0.205
1.753TyrTyr: 1.753 ± 0.271
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 70 proteins (17113 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski