Polaribacter sp. IC066

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Polaribacter; unclassified Polaribacter

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3473 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C6YBI1|A0A5C6YBI1_9FLAO Molecular chaperone DjiA OS=Polaribacter sp. IC066 OX=57032 GN=ES044_05975 PE=4 SV=1
MM1 pKa = 7.51TKK3 pKa = 10.66LKK5 pKa = 10.38FLFVALFCAALVLQTTSCSTNDD27 pKa = 3.89DD28 pKa = 3.93GPGGEE33 pKa = 4.16EE34 pKa = 3.92VGNDD38 pKa = 3.39VQITGISINPTSLSLEE54 pKa = 4.03EE55 pKa = 4.92GVTSNLVVSLEE66 pKa = 4.08PSNATGTVTWSSSNPAVATVDD87 pKa = 3.1SDD89 pKa = 4.05GLVSSISEE97 pKa = 4.13GTASIAAAIGTFSSVCDD114 pKa = 3.38VTVTKK119 pKa = 10.39EE120 pKa = 3.74AVIVDD125 pKa = 5.01DD126 pKa = 4.03ITLKK130 pKa = 10.99GSDD133 pKa = 3.97YY134 pKa = 11.26YY135 pKa = 10.34IIQLDD140 pKa = 3.94EE141 pKa = 4.78DD142 pKa = 4.27SYY144 pKa = 11.96SAIEE148 pKa = 4.2SKK150 pKa = 10.68VIQDD154 pKa = 3.92FRR156 pKa = 11.84PDD158 pKa = 3.39DD159 pKa = 3.72TTKK162 pKa = 10.66FLYY165 pKa = 10.37VWDD168 pKa = 3.99GTFEE172 pKa = 4.42GGNSVGNNFYY182 pKa = 11.16GLDD185 pKa = 3.71QDD187 pKa = 4.36WISLKK192 pKa = 10.46VGNVGWSGAGFSVGSAYY209 pKa = 10.56GDD211 pKa = 2.95IDD213 pKa = 4.0MTPMFDD219 pKa = 4.01NPEE222 pKa = 4.04DD223 pKa = 4.14YY224 pKa = 10.73YY225 pKa = 11.77LHH227 pKa = 7.24IGFKK231 pKa = 9.43TGQSASSYY239 pKa = 11.16LFILTDD245 pKa = 3.85GQAEE249 pKa = 4.07AKK251 pKa = 10.0IAIGNDD257 pKa = 3.17FNDD260 pKa = 4.73DD261 pKa = 3.31GTTYY265 pKa = 10.69TSYY268 pKa = 11.22KK269 pKa = 10.08QLTRR273 pKa = 11.84DD274 pKa = 3.73NTWNSIDD281 pKa = 4.29IPVTHH286 pKa = 7.27LNQLGLFYY294 pKa = 10.89NQTFQDD300 pKa = 3.76VNIFTMLAGGNQGTTLDD317 pKa = 3.44MDD319 pKa = 4.05AVFFYY324 pKa = 10.93KK325 pKa = 10.47KK326 pKa = 10.62

Molecular weight: 35.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C6YES7|A0A5C6YES7_9FLAO TonB-dependent receptor OS=Polaribacter sp. IC066 OX=57032 GN=ES044_02925 PE=3 SV=1
MM1 pKa = 7.56KK2 pKa = 10.32KK3 pKa = 10.58GIFILLGMLLMVSTIEE19 pKa = 4.22AKK21 pKa = 10.46KK22 pKa = 10.34SQNTANNFSFTYY34 pKa = 10.09AYY36 pKa = 10.8NNAVNFIEE44 pKa = 4.34NGVEE48 pKa = 4.71FFIFTNGDD56 pKa = 2.91FDD58 pKa = 5.92FDD60 pKa = 3.36TRR62 pKa = 11.84TNDD65 pKa = 2.72RR66 pKa = 11.84RR67 pKa = 11.84VRR69 pKa = 11.84IHH71 pKa = 7.09RR72 pKa = 11.84DD73 pKa = 2.98FNGRR77 pKa = 11.84IRR79 pKa = 11.84SIGNVHH85 pKa = 6.71LRR87 pKa = 11.84YY88 pKa = 9.93DD89 pKa = 3.28LRR91 pKa = 11.84GNVTRR96 pKa = 11.84IGAISIRR103 pKa = 11.84YY104 pKa = 8.14FRR106 pKa = 11.84GRR108 pKa = 11.84LTNVGDD114 pKa = 3.38LRR116 pKa = 11.84VRR118 pKa = 11.84YY119 pKa = 9.24NRR121 pKa = 11.84WEE123 pKa = 3.97YY124 pKa = 10.09PVFYY128 pKa = 10.92GNVRR132 pKa = 11.84NFYY135 pKa = 9.39YY136 pKa = 11.02NNGVRR141 pKa = 11.84FNVSFGDD148 pKa = 3.17ICNYY152 pKa = 9.57NDD154 pKa = 3.22TYY156 pKa = 11.07FFRR159 pKa = 11.84NDD161 pKa = 2.9FSRR164 pKa = 11.84NYY166 pKa = 8.03TQIRR170 pKa = 11.84EE171 pKa = 4.12DD172 pKa = 3.59RR173 pKa = 11.84NFYY176 pKa = 10.58YY177 pKa = 10.87YY178 pKa = 10.07QANPNARR185 pKa = 11.84IGKK188 pKa = 9.67RR189 pKa = 11.84STILKK194 pKa = 8.71RR195 pKa = 11.84RR196 pKa = 11.84KK197 pKa = 9.31PISVNNNNNRR207 pKa = 11.84RR208 pKa = 11.84KK209 pKa = 10.35NSATSSNNSYY219 pKa = 10.61RR220 pKa = 11.84KK221 pKa = 8.77PQRR224 pKa = 11.84TTNTRR229 pKa = 11.84NTAIKK234 pKa = 10.33RR235 pKa = 11.84STRR238 pKa = 11.84NSNTTSKK245 pKa = 9.8TDD247 pKa = 3.51ADD249 pKa = 4.01RR250 pKa = 11.84TPAIKK255 pKa = 9.53TEE257 pKa = 3.89RR258 pKa = 11.84EE259 pKa = 3.89TLKK262 pKa = 10.41EE263 pKa = 3.54RR264 pKa = 11.84RR265 pKa = 11.84SFKK268 pKa = 10.62KK269 pKa = 9.13STRR272 pKa = 11.84NEE274 pKa = 3.86AKK276 pKa = 9.78TLEE279 pKa = 4.28STRR282 pKa = 11.84NKK284 pKa = 10.72YY285 pKa = 7.47EE286 pKa = 3.84VKK288 pKa = 9.76TKK290 pKa = 9.8SRR292 pKa = 11.84RR293 pKa = 11.84NTRR296 pKa = 11.84NKK298 pKa = 10.21SDD300 pKa = 3.27RR301 pKa = 11.84SS302 pKa = 3.6

Molecular weight: 35.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3473

0

3473

1147573

24

2805

330.4

37.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.268 ± 0.039

0.689 ± 0.011

5.326 ± 0.028

6.594 ± 0.045

5.548 ± 0.029

6.057 ± 0.043

1.693 ± 0.018

8.472 ± 0.042

8.635 ± 0.05

9.252 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.069 ± 0.02

6.508 ± 0.049

3.027 ± 0.02

3.299 ± 0.022

3.213 ± 0.024

6.553 ± 0.031

5.889 ± 0.032

6.031 ± 0.032

0.995 ± 0.015

3.881 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski