Amino acid dipepetide frequency for Polaribacter sp. IC066

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.22AlaAla: 4.22 ± 0.083
0.479AlaCys: 0.479 ± 0.02
3.394AlaAsp: 3.394 ± 0.058
3.728AlaGlu: 3.728 ± 0.063
3.371AlaPhe: 3.371 ± 0.056
3.954AlaGly: 3.954 ± 0.073
1.052AlaHis: 1.052 ± 0.031
5.92AlaIle: 5.92 ± 0.087
4.938AlaLys: 4.938 ± 0.075
5.793AlaLeu: 5.793 ± 0.082
1.365AlaMet: 1.365 ± 0.034
3.541AlaAsn: 3.541 ± 0.064
1.701AlaPro: 1.701 ± 0.042
2.24AlaGln: 2.24 ± 0.046
1.905AlaArg: 1.905 ± 0.047
4.312AlaSer: 4.312 ± 0.07
3.921AlaThr: 3.921 ± 0.065
4.04AlaVal: 4.04 ± 0.061
0.532AlaTrp: 0.532 ± 0.023
2.276AlaTyr: 2.276 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
0.432CysAla: 0.432 ± 0.02
0.101CysCys: 0.101 ± 0.01
0.37CysAsp: 0.37 ± 0.019
0.38CysGlu: 0.38 ± 0.019
0.437CysPhe: 0.437 ± 0.02
0.57CysGly: 0.57 ± 0.024
0.165CysHis: 0.165 ± 0.012
0.567CysIle: 0.567 ± 0.023
0.522CysLys: 0.522 ± 0.02
0.571CysLeu: 0.571 ± 0.023
0.142CysMet: 0.142 ± 0.01
0.383CysAsn: 0.383 ± 0.022
0.24CysPro: 0.24 ± 0.015
0.196CysGln: 0.196 ± 0.013
0.187CysArg: 0.187 ± 0.013
0.514CysSer: 0.514 ± 0.024
0.396CysThr: 0.396 ± 0.019
0.397CysVal: 0.397 ± 0.018
0.058CysTrp: 0.058 ± 0.008
0.26CysTyr: 0.26 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
3.886AspAla: 3.886 ± 0.064
0.384AspCys: 0.384 ± 0.016
2.66AspAsp: 2.66 ± 0.063
3.616AspGlu: 3.616 ± 0.062
3.884AspPhe: 3.884 ± 0.062
3.297AspGly: 3.297 ± 0.066
0.803AspHis: 0.803 ± 0.03
4.416AspIle: 4.416 ± 0.065
4.351AspLys: 4.351 ± 0.066
5.157AspLeu: 5.157 ± 0.086
0.938AspMet: 0.938 ± 0.033
2.922AspAsn: 2.922 ± 0.056
1.329AspPro: 1.329 ± 0.038
1.306AspGln: 1.306 ± 0.033
1.658AspArg: 1.658 ± 0.045
3.038AspSer: 3.038 ± 0.052
2.848AspThr: 2.848 ± 0.048
3.737AspVal: 3.737 ± 0.058
0.703AspTrp: 0.703 ± 0.025
2.324AspTyr: 2.324 ± 0.047
0.0AspXaa: 0.0 ± 0.0
Glu
3.999GluAla: 3.999 ± 0.079
0.308GluCys: 0.308 ± 0.018
3.411GluAsp: 3.411 ± 0.052
4.856GluGlu: 4.856 ± 0.074
3.008GluPhe: 3.008 ± 0.045
3.307GluGly: 3.307 ± 0.055
1.107GluHis: 1.107 ± 0.034
6.261GluIle: 6.261 ± 0.082
6.645GluLys: 6.645 ± 0.095
6.014GluLeu: 6.014 ± 0.084
1.57GluMet: 1.57 ± 0.035
5.406GluAsn: 5.406 ± 0.071
1.318GluPro: 1.318 ± 0.033
2.095GluGln: 2.095 ± 0.042
2.314GluArg: 2.314 ± 0.046
3.237GluSer: 3.237 ± 0.059
3.883GluThr: 3.883 ± 0.059
4.178GluVal: 4.178 ± 0.061
0.571GluTrp: 0.571 ± 0.026
2.453GluTyr: 2.453 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
3.045PheAla: 3.045 ± 0.05
0.446PheCys: 0.446 ± 0.019
3.438PheAsp: 3.438 ± 0.058
3.364PheGlu: 3.364 ± 0.051
3.012PhePhe: 3.012 ± 0.058
3.836PheGly: 3.836 ± 0.064
0.823PheHis: 0.823 ± 0.027
4.455PheIle: 4.455 ± 0.083
4.456PheLys: 4.456 ± 0.076
5.281PheLeu: 5.281 ± 0.092
1.128PheMet: 1.128 ± 0.032
3.492PheAsn: 3.492 ± 0.061
1.564PhePro: 1.564 ± 0.039
1.507PheGln: 1.507 ± 0.035
1.65PheArg: 1.65 ± 0.037
4.571PheSer: 4.571 ± 0.066
3.459PheThr: 3.459 ± 0.052
3.064PheVal: 3.064 ± 0.06
0.571PheTrp: 0.571 ± 0.026
2.322PheTyr: 2.322 ± 0.049
0.0PheXaa: 0.0 ± 0.0
Gly
4.008GlyAla: 4.008 ± 0.07
0.465GlyCys: 0.465 ± 0.022
3.04GlyAsp: 3.04 ± 0.06
3.28GlyGlu: 3.28 ± 0.054
3.939GlyPhe: 3.939 ± 0.065
4.335GlyGly: 4.335 ± 0.082
0.976GlyHis: 0.976 ± 0.032
5.43GlyIle: 5.43 ± 0.075
4.912GlyLys: 4.912 ± 0.075
5.402GlyLeu: 5.402 ± 0.07
1.455GlyMet: 1.455 ± 0.039
3.603GlyAsn: 3.603 ± 0.065
1.122GlyPro: 1.122 ± 0.034
1.664GlyGln: 1.664 ± 0.039
1.957GlyArg: 1.957 ± 0.054
3.836GlySer: 3.836 ± 0.076
3.77GlyThr: 3.77 ± 0.072
4.151GlyVal: 4.151 ± 0.067
0.68GlyTrp: 0.68 ± 0.029
2.541GlyTyr: 2.541 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
0.911HisAla: 0.911 ± 0.03
0.139HisCys: 0.139 ± 0.01
0.731HisAsp: 0.731 ± 0.023
0.918HisGlu: 0.918 ± 0.03
1.085HisPhe: 1.085 ± 0.034
0.884HisGly: 0.884 ± 0.026
0.456HisHis: 0.456 ± 0.022
1.441HisIle: 1.441 ± 0.033
1.455HisLys: 1.455 ± 0.036
1.741HisLeu: 1.741 ± 0.042
0.279HisMet: 0.279 ± 0.015
0.927HisAsn: 0.927 ± 0.031
0.832HisPro: 0.832 ± 0.029
0.842HisGln: 0.842 ± 0.028
0.617HisArg: 0.617 ± 0.02
0.976HisSer: 0.976 ± 0.034
0.948HisThr: 0.948 ± 0.031
0.844HisVal: 0.844 ± 0.029
0.196HisTrp: 0.196 ± 0.014
0.708HisTyr: 0.708 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
6.007IleAla: 6.007 ± 0.094
0.663IleCys: 0.663 ± 0.025
5.067IleAsp: 5.067 ± 0.08
5.697IleGlu: 5.697 ± 0.079
3.981IlePhe: 3.981 ± 0.059
5.214IleGly: 5.214 ± 0.076
1.498IleHis: 1.498 ± 0.034
6.875IleIle: 6.875 ± 0.095
6.963IleLys: 6.963 ± 0.099
7.617IleLeu: 7.617 ± 0.097
1.346IleMet: 1.346 ± 0.041
5.287IleAsn: 5.287 ± 0.083
3.12IlePro: 3.12 ± 0.047
2.701IleGln: 2.701 ± 0.052
2.636IleArg: 2.636 ± 0.05
6.326IleSer: 6.326 ± 0.087
5.21IleThr: 5.21 ± 0.069
4.89IleVal: 4.89 ± 0.073
0.712IleTrp: 0.712 ± 0.028
2.913IleTyr: 2.913 ± 0.056
0.0IleXaa: 0.0 ± 0.0
Lys
5.002LysAla: 5.002 ± 0.084
0.35LysCys: 0.35 ± 0.019
4.652LysAsp: 4.652 ± 0.082
7.496LysGlu: 7.496 ± 0.107
3.302LysPhe: 3.302 ± 0.056
4.76LysGly: 4.76 ± 0.065
1.4LysHis: 1.4 ± 0.038
7.616LysIle: 7.616 ± 0.101
8.46LysLys: 8.46 ± 0.121
6.956LysLeu: 6.956 ± 0.085
2.158LysMet: 2.158 ± 0.047
6.624LysAsn: 6.624 ± 0.089
2.369LysPro: 2.369 ± 0.045
2.881LysGln: 2.881 ± 0.059
3.048LysArg: 3.048 ± 0.065
4.983LysSer: 4.983 ± 0.066
5.221LysThr: 5.221 ± 0.071
5.059LysVal: 5.059 ± 0.064
0.794LysTrp: 0.794 ± 0.024
3.223LysTyr: 3.223 ± 0.059
0.0LysXaa: 0.0 ± 0.0
Leu
5.587LeuAla: 5.587 ± 0.079
0.622LeuCys: 0.622 ± 0.021
4.84LeuAsp: 4.84 ± 0.075
6.665LeuGlu: 6.665 ± 0.086
5.194LeuPhe: 5.194 ± 0.085
5.628LeuGly: 5.628 ± 0.08
1.529LeuHis: 1.529 ± 0.04
7.433LeuIle: 7.433 ± 0.107
8.725LeuLys: 8.725 ± 0.104
8.642LeuLeu: 8.642 ± 0.131
1.954LeuMet: 1.954 ± 0.043
5.918LeuAsn: 5.918 ± 0.079
3.175LeuPro: 3.175 ± 0.055
3.33LeuGln: 3.33 ± 0.06
2.853LeuArg: 2.853 ± 0.048
6.358LeuSer: 6.358 ± 0.076
5.043LeuThr: 5.043 ± 0.07
5.253LeuVal: 5.253 ± 0.078
0.77LeuTrp: 0.77 ± 0.026
3.003LeuTyr: 3.003 ± 0.054
0.0LeuXaa: 0.0 ± 0.0
Met
1.478MetAla: 1.478 ± 0.035
0.137MetCys: 0.137 ± 0.013
1.018MetAsp: 1.018 ± 0.03
1.21MetGlu: 1.21 ± 0.034
0.879MetPhe: 0.879 ± 0.028
1.199MetGly: 1.199 ± 0.032
0.392MetHis: 0.392 ± 0.019
1.624MetIle: 1.624 ± 0.036
2.199MetLys: 2.199 ± 0.04
1.881MetLeu: 1.881 ± 0.045
0.568MetMet: 0.568 ± 0.02
1.377MetAsn: 1.377 ± 0.032
0.767MetPro: 0.767 ± 0.028
0.795MetGln: 0.795 ± 0.026
0.754MetArg: 0.754 ± 0.027
1.352MetSer: 1.352 ± 0.034
1.049MetThr: 1.049 ± 0.032
1.179MetVal: 1.179 ± 0.034
0.134MetTrp: 0.134 ± 0.011
0.701MetTyr: 0.701 ± 0.023
0.0MetXaa: 0.0 ± 0.0
Asn
3.996AsnAla: 3.996 ± 0.067
0.447AsnCys: 0.447 ± 0.02
3.185AsnAsp: 3.185 ± 0.058
3.818AsnGlu: 3.818 ± 0.061
3.654AsnPhe: 3.654 ± 0.055
4.034AsnGly: 4.034 ± 0.073
1.114AsnHis: 1.114 ± 0.032
5.287AsnIle: 5.287 ± 0.077
4.994AsnLys: 4.994 ± 0.08
6.13AsnLeu: 6.13 ± 0.083
1.206AsnMet: 1.206 ± 0.031
4.398AsnAsn: 4.398 ± 0.093
2.687AsnPro: 2.687 ± 0.054
2.382AsnGln: 2.382 ± 0.05
2.102AsnArg: 2.102 ± 0.05
4.393AsnSer: 4.393 ± 0.08
3.976AsnThr: 3.976 ± 0.072
3.578AsnVal: 3.578 ± 0.061
0.796AsnTrp: 0.796 ± 0.029
2.906AsnTyr: 2.906 ± 0.049
0.0AsnXaa: 0.0 ± 0.0
Pro
1.687ProAla: 1.687 ± 0.038
0.207ProCys: 0.207 ± 0.014
1.611ProAsp: 1.611 ± 0.043
2.427ProGlu: 2.427 ± 0.044
1.875ProPhe: 1.875 ± 0.037
1.482ProGly: 1.482 ± 0.046
0.533ProHis: 0.533 ± 0.021
2.674ProIle: 2.674 ± 0.052
2.633ProLys: 2.633 ± 0.053
2.721ProLeu: 2.721 ± 0.043
0.616ProMet: 0.616 ± 0.024
2.135ProAsn: 2.135 ± 0.05
0.666ProPro: 0.666 ± 0.023
0.937ProGln: 0.937 ± 0.029
0.861ProArg: 0.861 ± 0.028
1.867ProSer: 1.867 ± 0.05
1.914ProThr: 1.914 ± 0.041
1.928ProVal: 1.928 ± 0.043
0.3ProTrp: 0.3 ± 0.016
1.196ProTyr: 1.196 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
1.763GlnAla: 1.763 ± 0.035
0.16GlnCys: 0.16 ± 0.013
1.543GlnAsp: 1.543 ± 0.036
2.538GlnGlu: 2.538 ± 0.049
1.731GlnPhe: 1.731 ± 0.042
1.649GlnGly: 1.649 ± 0.038
0.558GlnHis: 0.558 ± 0.021
2.831GlnIle: 2.831 ± 0.051
3.193GlnLys: 3.193 ± 0.054
3.283GlnLeu: 3.283 ± 0.06
0.768GlnMet: 0.768 ± 0.024
2.326GlnAsn: 2.326 ± 0.055
0.897GlnPro: 0.897 ± 0.034
1.481GlnGln: 1.481 ± 0.041
1.141GlnArg: 1.141 ± 0.032
1.787GlnSer: 1.787 ± 0.041
1.923GlnThr: 1.923 ± 0.04
1.91GlnVal: 1.91 ± 0.04
0.303GlnTrp: 0.303 ± 0.015
1.21GlnTyr: 1.21 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
1.968ArgAla: 1.968 ± 0.039
0.184ArgCys: 0.184 ± 0.013
1.646ArgAsp: 1.646 ± 0.038
2.037ArgGlu: 2.037 ± 0.048
1.904ArgPhe: 1.904 ± 0.04
1.891ArgGly: 1.891 ± 0.043
0.526ArgHis: 0.526 ± 0.024
2.777ArgIle: 2.777 ± 0.054
2.947ArgLys: 2.947 ± 0.059
2.963ArgLeu: 2.963 ± 0.049
0.793ArgMet: 0.793 ± 0.026
2.144ArgAsn: 2.144 ± 0.041
0.919ArgPro: 0.919 ± 0.032
0.987ArgGln: 0.987 ± 0.027
1.119ArgArg: 1.119 ± 0.038
1.825ArgSer: 1.825 ± 0.039
1.823ArgThr: 1.823 ± 0.045
1.969ArgVal: 1.969 ± 0.037
0.309ArgTrp: 0.309 ± 0.017
1.401ArgTyr: 1.401 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
3.827SerAla: 3.827 ± 0.063
0.592SerCys: 0.592 ± 0.022
3.466SerAsp: 3.466 ± 0.06
4.167SerGlu: 4.167 ± 0.056
4.197SerPhe: 4.197 ± 0.059
4.269SerGly: 4.269 ± 0.069
0.966SerHis: 0.966 ± 0.032
5.571SerIle: 5.571 ± 0.071
5.643SerLys: 5.643 ± 0.076
6.292SerLeu: 6.292 ± 0.081
1.3SerMet: 1.3 ± 0.033
4.048SerAsn: 4.048 ± 0.071
1.853SerPro: 1.853 ± 0.044
2.013SerGln: 2.013 ± 0.043
1.929SerArg: 1.929 ± 0.047
4.413SerSer: 4.413 ± 0.072
3.592SerThr: 3.592 ± 0.058
3.866SerVal: 3.866 ± 0.058
0.763SerTrp: 0.763 ± 0.031
2.76SerTyr: 2.76 ± 0.05
0.0SerXaa: 0.0 ± 0.0
Thr
3.827ThrAla: 3.827 ± 0.059
0.344ThrCys: 0.344 ± 0.017
3.16ThrAsp: 3.16 ± 0.061
3.587ThrGlu: 3.587 ± 0.054
3.283ThrPhe: 3.283 ± 0.061
3.735ThrGly: 3.735 ± 0.065
1.0ThrHis: 1.0 ± 0.032
5.368ThrIle: 5.368 ± 0.073
4.582ThrLys: 4.582 ± 0.068
5.41ThrLeu: 5.41 ± 0.073
0.98ThrMet: 0.98 ± 0.03
3.67ThrAsn: 3.67 ± 0.071
2.212ThrPro: 2.212 ± 0.051
1.771ThrGln: 1.771 ± 0.045
1.572ThrArg: 1.572 ± 0.037
4.056ThrSer: 4.056 ± 0.067
3.772ThrThr: 3.772 ± 0.07
3.676ThrVal: 3.676 ± 0.055
0.565ThrTrp: 0.565 ± 0.025
2.321ThrTyr: 2.321 ± 0.046
0.0ThrXaa: 0.0 ± 0.0
Val
4.244ValAla: 4.244 ± 0.061
0.482ValCys: 0.482 ± 0.021
3.44ValAsp: 3.44 ± 0.062
3.42ValGlu: 3.42 ± 0.057
3.624ValPhe: 3.624 ± 0.06
3.64ValGly: 3.64 ± 0.066
1.07ValHis: 1.07 ± 0.033
4.747ValIle: 4.747 ± 0.071
4.577ValLys: 4.577 ± 0.075
6.115ValLeu: 6.115 ± 0.082
1.176ValMet: 1.176 ± 0.038
3.542ValAsn: 3.542 ± 0.065
1.935ValPro: 1.935 ± 0.043
1.816ValGln: 1.816 ± 0.045
1.976ValArg: 1.976 ± 0.044
4.474ValSer: 4.474 ± 0.066
3.324ValThr: 3.324 ± 0.059
3.915ValVal: 3.915 ± 0.07
0.592ValTrp: 0.592 ± 0.023
2.202ValTyr: 2.202 ± 0.044
0.0ValXaa: 0.0 ± 0.0
Trp
0.544TrpAla: 0.544 ± 0.026
0.08TrpCys: 0.08 ± 0.007
0.535TrpAsp: 0.535 ± 0.026
0.625TrpGlu: 0.625 ± 0.028
0.633TrpPhe: 0.633 ± 0.029
0.607TrpGly: 0.607 ± 0.026
0.2TrpHis: 0.2 ± 0.013
0.756TrpIle: 0.756 ± 0.028
0.757TrpLys: 0.757 ± 0.028
0.929TrpLeu: 0.929 ± 0.03
0.285TrpMet: 0.285 ± 0.015
0.732TrpAsn: 0.732 ± 0.024
0.196TrpPro: 0.196 ± 0.015
0.394TrpGln: 0.394 ± 0.02
0.419TrpArg: 0.419 ± 0.019
0.652TrpSer: 0.652 ± 0.029
0.509TrpThr: 0.509 ± 0.023
0.584TrpVal: 0.584 ± 0.025
0.134TrpTrp: 0.134 ± 0.012
0.377TrpTyr: 0.377 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.252TyrAla: 2.252 ± 0.045
0.297TyrCys: 0.297 ± 0.017
2.051TyrAsp: 2.051 ± 0.047
2.112TyrGlu: 2.112 ± 0.044
2.493TyrPhe: 2.493 ± 0.047
2.275TyrGly: 2.275 ± 0.05
0.762TyrHis: 0.762 ± 0.024
2.674TyrIle: 2.674 ± 0.051
3.399TyrLys: 3.399 ± 0.058
3.626TyrLeu: 3.626 ± 0.053
0.662TyrMet: 0.662 ± 0.025
2.608TyrAsn: 2.608 ± 0.047
1.37TyrPro: 1.37 ± 0.035
1.657TyrGln: 1.657 ± 0.036
1.434TyrArg: 1.434 ± 0.037
2.558TyrSer: 2.558 ± 0.048
2.307TyrThr: 2.307 ± 0.049
2.093TyrVal: 2.093 ± 0.041
0.465TyrTrp: 0.465 ± 0.022
1.71TyrTyr: 1.71 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3473 proteins (1147573 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski