Amino acid dipepetide frequency for Clostridium phage phi3626

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.327AlaAla: 2.327 ± 0.475
0.582AlaCys: 0.582 ± 0.373
1.552AlaAsp: 1.552 ± 0.391
3.976AlaGlu: 3.976 ± 0.614
2.036AlaPhe: 2.036 ± 0.354
3.394AlaGly: 3.394 ± 0.648
0.776AlaHis: 0.776 ± 0.263
4.558AlaIle: 4.558 ± 0.648
6.012AlaLys: 6.012 ± 0.742
4.17AlaLeu: 4.17 ± 0.736
2.133AlaMet: 2.133 ± 0.501
3.2AlaAsn: 3.2 ± 0.595
0.582AlaPro: 0.582 ± 0.218
0.873AlaGln: 0.873 ± 0.269
2.812AlaArg: 2.812 ± 0.488
2.036AlaSer: 2.036 ± 0.43
3.394AlaThr: 3.394 ± 0.649
2.23AlaVal: 2.23 ± 0.473
0.582AlaTrp: 0.582 ± 0.225
2.618AlaTyr: 2.618 ± 0.531
0.0AlaXaa: 0.0 ± 0.0
Cys
0.291CysAla: 0.291 ± 0.187
0.291CysCys: 0.291 ± 0.173
0.97CysAsp: 0.97 ± 0.335
0.97CysGlu: 0.97 ± 0.307
0.291CysPhe: 0.291 ± 0.177
1.164CysGly: 1.164 ± 0.366
0.291CysHis: 0.291 ± 0.184
0.776CysIle: 0.776 ± 0.282
1.455CysLys: 1.455 ± 0.392
0.679CysLeu: 0.679 ± 0.311
0.291CysMet: 0.291 ± 0.179
0.97CysAsn: 0.97 ± 0.37
0.097CysPro: 0.097 ± 0.1
0.194CysGln: 0.194 ± 0.132
1.067CysArg: 1.067 ± 0.353
0.776CysSer: 0.776 ± 0.294
0.97CysThr: 0.97 ± 0.356
0.873CysVal: 0.873 ± 0.322
0.291CysTrp: 0.291 ± 0.152
0.679CysTyr: 0.679 ± 0.309
0.0CysXaa: 0.0 ± 0.0
Asp
1.358AspAla: 1.358 ± 0.367
0.873AspCys: 0.873 ± 0.315
2.909AspAsp: 2.909 ± 0.542
4.364AspGlu: 4.364 ± 0.765
2.812AspPhe: 2.812 ± 0.561
4.073AspGly: 4.073 ± 0.543
0.388AspHis: 0.388 ± 0.23
5.528AspIle: 5.528 ± 1.016
6.206AspLys: 6.206 ± 0.733
5.431AspLeu: 5.431 ± 0.646
1.358AspMet: 1.358 ± 0.411
3.782AspAsn: 3.782 ± 0.701
1.261AspPro: 1.261 ± 0.497
0.679AspGln: 0.679 ± 0.268
2.327AspArg: 2.327 ± 0.452
3.297AspSer: 3.297 ± 0.555
1.746AspThr: 1.746 ± 0.331
3.976AspVal: 3.976 ± 0.566
0.485AspTrp: 0.485 ± 0.188
2.521AspTyr: 2.521 ± 0.442
0.0AspXaa: 0.0 ± 0.0
Glu
3.976GluAla: 3.976 ± 0.692
1.552GluCys: 1.552 ± 0.409
4.073GluAsp: 4.073 ± 0.596
9.407GluGlu: 9.407 ± 1.753
3.782GluPhe: 3.782 ± 0.665
5.721GluGly: 5.721 ± 0.8
1.358GluHis: 1.358 ± 0.302
8.049GluIle: 8.049 ± 1.165
9.019GluLys: 9.019 ± 1.423
9.116GluLeu: 9.116 ± 0.659
2.036GluMet: 2.036 ± 0.489
6.885GluAsn: 6.885 ± 0.8
1.455GluPro: 1.455 ± 0.477
1.939GluGln: 1.939 ± 0.524
4.073GluArg: 4.073 ± 0.839
3.782GluSer: 3.782 ± 0.736
4.364GluThr: 4.364 ± 0.633
5.14GluVal: 5.14 ± 0.896
1.164GluTrp: 1.164 ± 0.394
3.2GluTyr: 3.2 ± 0.657
0.0GluXaa: 0.0 ± 0.0
Phe
1.455PheAla: 1.455 ± 0.354
0.291PheCys: 0.291 ± 0.182
3.006PheAsp: 3.006 ± 0.583
4.267PheGlu: 4.267 ± 0.52
0.97PhePhe: 0.97 ± 0.222
3.2PheGly: 3.2 ± 0.55
0.097PheHis: 0.097 ± 0.1
4.17PheIle: 4.17 ± 0.537
4.17PheLys: 4.17 ± 0.628
3.103PheLeu: 3.103 ± 0.563
0.873PheMet: 0.873 ± 0.283
3.297PheAsn: 3.297 ± 0.613
0.679PhePro: 0.679 ± 0.209
1.261PheGln: 1.261 ± 0.468
1.358PheArg: 1.358 ± 0.393
2.23PheSer: 2.23 ± 0.443
2.133PheThr: 2.133 ± 0.712
2.133PheVal: 2.133 ± 0.429
0.679PheTrp: 0.679 ± 0.34
1.552PheTyr: 1.552 ± 0.393
0.0PheXaa: 0.0 ± 0.0
Gly
3.2GlyAla: 3.2 ± 0.684
0.679GlyCys: 0.679 ± 0.289
4.073GlyAsp: 4.073 ± 0.798
4.073GlyGlu: 4.073 ± 0.627
2.812GlyPhe: 2.812 ± 0.538
4.17GlyGly: 4.17 ± 0.892
0.679GlyHis: 0.679 ± 0.282
5.528GlyIle: 5.528 ± 0.889
6.594GlyLys: 6.594 ± 0.756
5.237GlyLeu: 5.237 ± 0.786
1.843GlyMet: 1.843 ± 0.444
3.685GlyAsn: 3.685 ± 0.63
0.291GlyPro: 0.291 ± 0.154
1.843GlyGln: 1.843 ± 0.431
2.327GlyArg: 2.327 ± 0.599
2.618GlySer: 2.618 ± 0.564
3.491GlyThr: 3.491 ± 0.613
4.946GlyVal: 4.946 ± 0.806
0.97GlyTrp: 0.97 ± 0.366
2.909GlyTyr: 2.909 ± 0.538
0.0GlyXaa: 0.0 ± 0.0
His
0.582HisAla: 0.582 ± 0.275
0.291HisCys: 0.291 ± 0.164
0.776HisAsp: 0.776 ± 0.293
1.164HisGlu: 1.164 ± 0.326
0.388HisPhe: 0.388 ± 0.172
0.873HisGly: 0.873 ± 0.244
0.194HisHis: 0.194 ± 0.167
0.873HisIle: 0.873 ± 0.307
1.067HisLys: 1.067 ± 0.344
0.97HisLeu: 0.97 ± 0.26
0.097HisMet: 0.097 ± 0.107
0.776HisAsn: 0.776 ± 0.247
0.194HisPro: 0.194 ± 0.117
0.194HisGln: 0.194 ± 0.158
0.485HisArg: 0.485 ± 0.224
0.776HisSer: 0.776 ± 0.24
0.291HisThr: 0.291 ± 0.149
0.388HisVal: 0.388 ± 0.228
0.194HisTrp: 0.194 ± 0.137
0.388HisTyr: 0.388 ± 0.188
0.0HisXaa: 0.0 ± 0.0
Ile
4.17IleAla: 4.17 ± 0.773
1.164IleCys: 1.164 ± 0.313
6.4IleAsp: 6.4 ± 0.572
7.467IleGlu: 7.467 ± 0.986
3.588IlePhe: 3.588 ± 0.675
6.012IleGly: 6.012 ± 0.988
0.873IleHis: 0.873 ± 0.319
6.206IleIle: 6.206 ± 0.748
9.116IleLys: 9.116 ± 0.842
6.885IleLeu: 6.885 ± 0.85
2.23IleMet: 2.23 ± 0.407
6.885IleAsn: 6.885 ± 0.782
2.424IlePro: 2.424 ± 0.328
2.424IleGln: 2.424 ± 0.457
3.879IleArg: 3.879 ± 0.535
5.721IleSer: 5.721 ± 0.865
3.782IleThr: 3.782 ± 0.735
4.461IleVal: 4.461 ± 0.535
0.776IleTrp: 0.776 ± 0.238
3.006IleTyr: 3.006 ± 0.6
0.0IleXaa: 0.0 ± 0.0
Lys
5.625LysAla: 5.625 ± 0.925
1.164LysCys: 1.164 ± 0.291
4.849LysAsp: 4.849 ± 0.683
11.637LysGlu: 11.637 ± 1.479
4.849LysPhe: 4.849 ± 0.597
4.946LysGly: 4.946 ± 0.679
1.164LysHis: 1.164 ± 0.431
9.891LysIle: 9.891 ± 1.145
9.697LysLys: 9.697 ± 1.305
8.825LysLeu: 8.825 ± 0.952
2.521LysMet: 2.521 ± 0.564
6.303LysAsn: 6.303 ± 0.676
1.843LysPro: 1.843 ± 0.363
2.909LysGln: 2.909 ± 0.523
5.528LysArg: 5.528 ± 0.745
6.982LysSer: 6.982 ± 0.926
5.625LysThr: 5.625 ± 0.89
4.946LysVal: 4.946 ± 0.649
0.485LysTrp: 0.485 ± 0.253
4.849LysTyr: 4.849 ± 0.793
0.0LysXaa: 0.0 ± 0.0
Leu
5.043LeuAla: 5.043 ± 0.785
0.97LeuCys: 0.97 ± 0.399
5.431LeuAsp: 5.431 ± 0.559
8.728LeuGlu: 8.728 ± 0.955
2.327LeuPhe: 2.327 ± 0.434
5.14LeuGly: 5.14 ± 0.666
0.388LeuHis: 0.388 ± 0.184
6.594LeuIle: 6.594 ± 0.808
7.952LeuLys: 7.952 ± 0.794
5.818LeuLeu: 5.818 ± 0.859
2.424LeuMet: 2.424 ± 0.437
7.176LeuAsn: 7.176 ± 0.853
2.23LeuPro: 2.23 ± 0.536
1.843LeuGln: 1.843 ± 0.423
4.849LeuArg: 4.849 ± 1.127
5.431LeuSer: 5.431 ± 0.827
4.752LeuThr: 4.752 ± 0.788
3.976LeuVal: 3.976 ± 0.52
0.388LeuTrp: 0.388 ± 0.221
3.491LeuTyr: 3.491 ± 0.654
0.0LeuXaa: 0.0 ± 0.0
Met
2.521MetAla: 2.521 ± 0.637
0.097MetCys: 0.097 ± 0.1
1.358MetAsp: 1.358 ± 0.415
1.552MetGlu: 1.552 ± 0.469
0.97MetPhe: 0.97 ± 0.346
1.067MetGly: 1.067 ± 0.345
0.0MetHis: 0.0 ± 0.0
1.939MetIle: 1.939 ± 0.455
3.297MetLys: 3.297 ± 0.662
1.939MetLeu: 1.939 ± 0.348
0.388MetMet: 0.388 ± 0.232
1.067MetAsn: 1.067 ± 0.308
0.582MetPro: 0.582 ± 0.249
0.485MetGln: 0.485 ± 0.233
1.455MetArg: 1.455 ± 0.385
1.649MetSer: 1.649 ± 0.405
0.873MetThr: 0.873 ± 0.309
1.552MetVal: 1.552 ± 0.444
0.0MetTrp: 0.0 ± 0.0
0.679MetTyr: 0.679 ± 0.232
0.0MetXaa: 0.0 ± 0.0
Asn
3.976AsnAla: 3.976 ± 0.671
0.776AsnCys: 0.776 ± 0.297
3.491AsnAsp: 3.491 ± 0.587
4.849AsnGlu: 4.849 ± 0.705
3.394AsnPhe: 3.394 ± 0.548
5.721AsnGly: 5.721 ± 1.343
0.582AsnHis: 0.582 ± 0.254
5.431AsnIle: 5.431 ± 0.791
8.825AsnLys: 8.825 ± 1.136
5.818AsnLeu: 5.818 ± 0.592
1.164AsnMet: 1.164 ± 0.241
5.915AsnAsn: 5.915 ± 1.088
2.036AsnPro: 2.036 ± 0.391
1.552AsnGln: 1.552 ± 0.405
1.843AsnArg: 1.843 ± 0.531
6.594AsnSer: 6.594 ± 0.731
4.364AsnThr: 4.364 ± 0.534
3.006AsnVal: 3.006 ± 0.535
0.97AsnTrp: 0.97 ± 0.272
2.521AsnTyr: 2.521 ± 0.476
0.0AsnXaa: 0.0 ± 0.0
Pro
1.067ProAla: 1.067 ± 0.272
0.194ProCys: 0.194 ± 0.133
0.97ProAsp: 0.97 ± 0.343
2.133ProGlu: 2.133 ± 0.651
0.873ProPhe: 0.873 ± 0.283
0.97ProGly: 0.97 ± 0.288
0.291ProHis: 0.291 ± 0.156
3.2ProIle: 3.2 ± 0.594
1.455ProLys: 1.455 ± 0.339
2.23ProLeu: 2.23 ± 0.484
0.194ProMet: 0.194 ± 0.124
1.746ProAsn: 1.746 ± 0.397
0.776ProPro: 0.776 ± 0.316
0.582ProGln: 0.582 ± 0.275
0.388ProArg: 0.388 ± 0.19
1.261ProSer: 1.261 ± 0.488
1.164ProThr: 1.164 ± 0.383
1.649ProVal: 1.649 ± 0.454
0.0ProTrp: 0.0 ± 0.0
0.776ProTyr: 0.776 ± 0.3
0.0ProXaa: 0.0 ± 0.0
Gln
1.358GlnAla: 1.358 ± 0.357
0.291GlnCys: 0.291 ± 0.199
1.261GlnAsp: 1.261 ± 0.501
2.133GlnGlu: 2.133 ± 0.483
0.776GlnPhe: 0.776 ± 0.246
1.164GlnGly: 1.164 ± 0.318
0.0GlnHis: 0.0 ± 0.0
1.843GlnIle: 1.843 ± 0.391
2.424GlnLys: 2.424 ± 0.539
2.424GlnLeu: 2.424 ± 0.426
0.776GlnMet: 0.776 ± 0.295
2.036GlnAsn: 2.036 ± 0.371
0.388GlnPro: 0.388 ± 0.161
1.164GlnGln: 1.164 ± 0.308
1.067GlnArg: 1.067 ± 0.41
2.23GlnSer: 2.23 ± 0.651
1.067GlnThr: 1.067 ± 0.345
1.067GlnVal: 1.067 ± 0.252
0.097GlnTrp: 0.097 ± 0.101
1.455GlnTyr: 1.455 ± 0.433
0.0GlnXaa: 0.0 ± 0.0
Arg
3.103ArgAla: 3.103 ± 0.726
0.873ArgCys: 0.873 ± 0.309
2.909ArgAsp: 2.909 ± 0.424
4.17ArgGlu: 4.17 ± 0.66
1.843ArgPhe: 1.843 ± 0.508
2.133ArgGly: 2.133 ± 0.448
0.679ArgHis: 0.679 ± 0.252
3.394ArgIle: 3.394 ± 0.61
5.721ArgLys: 5.721 ± 0.958
3.2ArgLeu: 3.2 ± 0.721
0.776ArgMet: 0.776 ± 0.233
2.133ArgAsn: 2.133 ± 0.428
1.358ArgPro: 1.358 ± 0.439
1.164ArgGln: 1.164 ± 0.3
2.327ArgArg: 2.327 ± 0.535
1.358ArgSer: 1.358 ± 0.567
1.649ArgThr: 1.649 ± 0.367
2.521ArgVal: 2.521 ± 0.447
0.388ArgTrp: 0.388 ± 0.203
1.552ArgTyr: 1.552 ± 0.454
0.0ArgXaa: 0.0 ± 0.0
Ser
1.358SerAla: 1.358 ± 0.53
0.97SerCys: 0.97 ± 0.4
3.782SerAsp: 3.782 ± 0.676
5.043SerGlu: 5.043 ± 0.745
2.618SerPhe: 2.618 ± 0.577
2.909SerGly: 2.909 ± 0.608
0.97SerHis: 0.97 ± 0.316
5.915SerIle: 5.915 ± 0.812
7.564SerLys: 7.564 ± 0.978
5.528SerLeu: 5.528 ± 0.866
1.939SerMet: 1.939 ± 0.397
4.752SerAsn: 4.752 ± 0.649
1.552SerPro: 1.552 ± 0.434
1.261SerGln: 1.261 ± 0.339
1.939SerArg: 1.939 ± 0.493
3.297SerSer: 3.297 ± 0.486
1.843SerThr: 1.843 ± 0.361
1.939SerVal: 1.939 ± 0.435
0.485SerTrp: 0.485 ± 0.208
2.424SerTyr: 2.424 ± 0.462
0.0SerXaa: 0.0 ± 0.0
Thr
2.715ThrAla: 2.715 ± 0.709
0.291ThrCys: 0.291 ± 0.161
1.746ThrAsp: 1.746 ± 0.474
2.909ThrGlu: 2.909 ± 0.498
2.036ThrPhe: 2.036 ± 0.409
3.782ThrGly: 3.782 ± 0.641
0.388ThrHis: 0.388 ± 0.192
5.043ThrIle: 5.043 ± 0.812
4.946ThrLys: 4.946 ± 0.713
4.364ThrLeu: 4.364 ± 0.786
0.873ThrMet: 0.873 ± 0.296
4.073ThrAsn: 4.073 ± 0.654
1.746ThrPro: 1.746 ± 0.423
2.618ThrGln: 2.618 ± 0.581
1.358ThrArg: 1.358 ± 0.306
2.521ThrSer: 2.521 ± 0.569
2.909ThrThr: 2.909 ± 0.47
3.782ThrVal: 3.782 ± 0.491
0.291ThrTrp: 0.291 ± 0.158
2.133ThrTyr: 2.133 ± 0.663
0.0ThrXaa: 0.0 ± 0.0
Val
3.588ValAla: 3.588 ± 0.428
0.776ValCys: 0.776 ± 0.433
2.909ValAsp: 2.909 ± 0.473
5.043ValGlu: 5.043 ± 0.888
2.036ValPhe: 2.036 ± 0.468
2.618ValGly: 2.618 ± 0.514
0.582ValHis: 0.582 ± 0.232
4.752ValIle: 4.752 ± 0.655
4.752ValLys: 4.752 ± 0.543
4.655ValLeu: 4.655 ± 0.747
0.582ValMet: 0.582 ± 0.263
4.752ValAsn: 4.752 ± 0.879
1.455ValPro: 1.455 ± 0.378
1.358ValGln: 1.358 ± 0.272
1.939ValArg: 1.939 ± 0.463
3.103ValSer: 3.103 ± 0.498
3.394ValThr: 3.394 ± 0.617
2.909ValVal: 2.909 ± 0.49
0.776ValTrp: 0.776 ± 0.238
2.23ValTyr: 2.23 ± 0.497
0.0ValXaa: 0.0 ± 0.0
Trp
0.679TrpAla: 0.679 ± 0.282
0.097TrpCys: 0.097 ± 0.094
0.582TrpAsp: 0.582 ± 0.269
1.164TrpGlu: 1.164 ± 0.31
0.485TrpPhe: 0.485 ± 0.201
0.873TrpGly: 0.873 ± 0.36
0.0TrpHis: 0.0 ± 0.0
0.388TrpIle: 0.388 ± 0.167
0.679TrpLys: 0.679 ± 0.261
0.776TrpLeu: 0.776 ± 0.262
0.0TrpMet: 0.0 ± 0.0
0.291TrpAsn: 0.291 ± 0.152
0.0TrpPro: 0.0 ± 0.0
0.388TrpGln: 0.388 ± 0.157
0.582TrpArg: 0.582 ± 0.183
0.388TrpSer: 0.388 ± 0.176
0.485TrpThr: 0.485 ± 0.33
0.97TrpVal: 0.97 ± 0.356
0.097TrpTrp: 0.097 ± 0.093
0.485TrpTyr: 0.485 ± 0.208
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.455TyrAla: 1.455 ± 0.366
1.164TyrCys: 1.164 ± 0.375
2.327TyrAsp: 2.327 ± 0.446
5.043TyrGlu: 5.043 ± 0.79
2.036TyrPhe: 2.036 ± 0.391
2.036TyrGly: 2.036 ± 0.488
1.261TyrHis: 1.261 ± 0.358
3.394TyrIle: 3.394 ± 0.626
3.685TyrLys: 3.685 ± 0.816
3.782TyrLeu: 3.782 ± 0.727
0.873TyrMet: 0.873 ± 0.326
3.103TyrAsn: 3.103 ± 0.444
0.97TyrPro: 0.97 ± 0.337
0.291TyrGln: 0.291 ± 0.182
1.649TyrArg: 1.649 ± 0.359
2.036TyrSer: 2.036 ± 0.553
2.23TyrThr: 2.23 ± 0.413
1.843TyrVal: 1.843 ± 0.579
0.291TyrTrp: 0.291 ± 0.203
2.23TyrTyr: 2.23 ± 0.409
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (10313 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski