Bifidobacterium primatium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2029 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2M9HBD6|A0A2M9HBD6_9BIFI Amino acid ABC transporter substrate-binding protein OS=Bifidobacterium primatium OX=2045438 GN=CS006_03055 PE=3 SV=1
MM1 pKa = 7.52IGGIVALAIIIAVVLAVIGVVGGHH25 pKa = 6.25SDD27 pKa = 3.48DD28 pKa = 3.37STYY31 pKa = 11.63ADD33 pKa = 3.7GQQGYY38 pKa = 10.33DD39 pKa = 3.53SVPFDD44 pKa = 5.49DD45 pKa = 5.66PYY47 pKa = 11.73QMQRR51 pKa = 11.84DD52 pKa = 3.72ISSITGSSCLWSDD65 pKa = 2.92IGSYY69 pKa = 10.19SALGGDD75 pKa = 4.62SEE77 pKa = 4.72ASSIPGLDD85 pKa = 3.29GAYY88 pKa = 8.94TCGDD92 pKa = 3.88DD93 pKa = 5.44EE94 pKa = 4.79ILLMFDD100 pKa = 3.34TTSDD104 pKa = 3.15TRR106 pKa = 11.84QMVTTLEE113 pKa = 4.0RR114 pKa = 11.84SLAQGDD120 pKa = 4.0DD121 pKa = 3.82TFDD124 pKa = 3.47TTVTDD129 pKa = 3.38SLGDD133 pKa = 3.6YY134 pKa = 10.88AVVHH138 pKa = 7.44DD139 pKa = 4.83DD140 pKa = 2.69TWMVIGKK147 pKa = 8.77NADD150 pKa = 3.07MKK152 pKa = 10.64QLHH155 pKa = 6.56DD156 pKa = 3.46AWGGDD161 pKa = 3.49MQRR164 pKa = 11.84LDD166 pKa = 4.0EE167 pKa = 4.76LADD170 pKa = 3.58SGSRR174 pKa = 11.84DD175 pKa = 3.72TII177 pKa = 3.73

Molecular weight:
18.8 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2M9HBS7|A0A2M9HBS7_9BIFI Non-specific serine/threonine protein kinase OS=Bifidobacterium primatium OX=2045438 GN=CS006_03860 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.79MKK15 pKa = 9.36HH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84ALINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.79GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight:
5.36 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2029

0

2029

759016

24

3146

374.1

40.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.098 ± 0.082

0.876 ± 0.017

6.793 ± 0.053

5.581 ± 0.055

3.468 ± 0.037

8.322 ± 0.046

2.127 ± 0.026

5.314 ± 0.041

3.994 ± 0.053

8.631 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.573 ± 0.025

3.294 ± 0.038

4.595 ± 0.036

3.05 ± 0.03

6.155 ± 0.069

6.034 ± 0.056

6.091 ± 0.049

7.914 ± 0.044

1.309 ± 0.022

2.781 ± 0.03

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski