Amino acid dipepetide frequency for Shigella phage DS8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.839AlaAla: 8.839 ± 1.585
0.884AlaCys: 0.884 ± 0.296
4.641AlaAsp: 4.641 ± 0.652
6.85AlaGlu: 6.85 ± 0.909
3.094AlaPhe: 3.094 ± 0.519
6.998AlaGly: 6.998 ± 0.907
0.589AlaHis: 0.589 ± 0.284
6.114AlaIle: 6.114 ± 0.729
6.04AlaLys: 6.04 ± 0.753
7.145AlaLeu: 7.145 ± 0.945
3.388AlaMet: 3.388 ± 0.586
4.42AlaAsn: 4.42 ± 0.644
2.283AlaPro: 2.283 ± 0.387
4.051AlaGln: 4.051 ± 0.724
5.966AlaArg: 5.966 ± 0.536
4.493AlaSer: 4.493 ± 0.802
5.009AlaThr: 5.009 ± 0.746
5.156AlaVal: 5.156 ± 0.696
1.4AlaTrp: 1.4 ± 0.388
2.946AlaTyr: 2.946 ± 0.442
0.0AlaXaa: 0.0 ± 0.0
Cys
1.179CysAla: 1.179 ± 0.294
0.516CysCys: 0.516 ± 0.22
1.031CysAsp: 1.031 ± 0.351
1.031CysGlu: 1.031 ± 0.294
0.589CysPhe: 0.589 ± 0.236
1.621CysGly: 1.621 ± 0.408
0.442CysHis: 0.442 ± 0.163
0.442CysIle: 0.442 ± 0.198
1.031CysLys: 1.031 ± 0.297
0.958CysLeu: 0.958 ± 0.276
0.442CysMet: 0.442 ± 0.203
0.81CysAsn: 0.81 ± 0.257
0.589CysPro: 0.589 ± 0.187
0.589CysGln: 0.589 ± 0.234
0.589CysArg: 0.589 ± 0.201
1.031CysSer: 1.031 ± 0.333
0.442CysThr: 0.442 ± 0.154
0.663CysVal: 0.663 ± 0.244
0.368CysTrp: 0.368 ± 0.147
0.516CysTyr: 0.516 ± 0.201
0.0CysXaa: 0.0 ± 0.0
Asp
6.335AspAla: 6.335 ± 0.708
0.737AspCys: 0.737 ± 0.232
5.23AspAsp: 5.23 ± 0.795
4.935AspGlu: 4.935 ± 0.718
2.725AspPhe: 2.725 ± 0.445
6.114AspGly: 6.114 ± 0.923
1.105AspHis: 1.105 ± 0.26
4.567AspIle: 4.567 ± 0.556
3.388AspLys: 3.388 ± 0.672
2.946AspLeu: 2.946 ± 0.411
2.578AspMet: 2.578 ± 0.449
2.578AspAsn: 2.578 ± 0.479
1.915AspPro: 1.915 ± 0.417
1.179AspGln: 1.179 ± 0.299
2.652AspArg: 2.652 ± 0.432
3.315AspSer: 3.315 ± 0.559
2.652AspThr: 2.652 ± 0.544
4.567AspVal: 4.567 ± 0.614
0.589AspTrp: 0.589 ± 0.196
2.725AspTyr: 2.725 ± 0.387
0.0AspXaa: 0.0 ± 0.0
Glu
6.187GluAla: 6.187 ± 0.79
1.179GluCys: 1.179 ± 0.362
4.051GluAsp: 4.051 ± 0.762
4.641GluGlu: 4.641 ± 0.635
1.989GluPhe: 1.989 ± 0.428
2.799GluGly: 2.799 ± 0.457
0.884GluHis: 0.884 ± 0.264
4.641GluIle: 4.641 ± 0.512
4.493GluLys: 4.493 ± 0.679
6.114GluLeu: 6.114 ± 0.64
2.725GluMet: 2.725 ± 0.487
2.799GluAsn: 2.799 ± 0.41
1.841GluPro: 1.841 ± 0.353
4.051GluGln: 4.051 ± 0.573
3.167GluArg: 3.167 ± 0.535
3.609GluSer: 3.609 ± 0.416
3.02GluThr: 3.02 ± 0.463
4.641GluVal: 4.641 ± 0.593
1.547GluTrp: 1.547 ± 0.338
3.241GluTyr: 3.241 ± 0.401
0.0GluXaa: 0.0 ± 0.0
Phe
2.136PheAla: 2.136 ± 0.438
0.516PheCys: 0.516 ± 0.201
3.167PheAsp: 3.167 ± 0.397
2.21PheGlu: 2.21 ± 0.432
1.252PhePhe: 1.252 ± 0.28
2.652PheGly: 2.652 ± 0.377
0.737PheHis: 0.737 ± 0.22
2.652PheIle: 2.652 ± 0.521
2.357PheLys: 2.357 ± 0.462
1.915PheLeu: 1.915 ± 0.334
1.547PheMet: 1.547 ± 0.334
2.431PheAsn: 2.431 ± 0.55
0.958PhePro: 0.958 ± 0.244
0.958PheGln: 0.958 ± 0.293
2.21PheArg: 2.21 ± 0.428
2.283PheSer: 2.283 ± 0.354
1.989PheThr: 1.989 ± 0.465
1.768PheVal: 1.768 ± 0.407
0.663PheTrp: 0.663 ± 0.24
1.252PheTyr: 1.252 ± 0.349
0.0PheXaa: 0.0 ± 0.0
Gly
5.819GlyAla: 5.819 ± 0.795
1.179GlyCys: 1.179 ± 0.311
4.493GlyAsp: 4.493 ± 0.538
4.641GlyGlu: 4.641 ± 0.659
2.504GlyPhe: 2.504 ± 0.46
5.082GlyGly: 5.082 ± 0.591
0.958GlyHis: 0.958 ± 0.253
4.199GlyIle: 4.199 ± 0.517
5.377GlyLys: 5.377 ± 0.768
4.272GlyLeu: 4.272 ± 0.552
1.989GlyMet: 1.989 ± 0.405
2.946GlyAsn: 2.946 ± 0.5
0.589GlyPro: 0.589 ± 0.215
2.431GlyGln: 2.431 ± 0.501
3.609GlyArg: 3.609 ± 0.404
4.346GlySer: 4.346 ± 0.566
3.978GlyThr: 3.978 ± 0.741
4.199GlyVal: 4.199 ± 0.584
1.326GlyTrp: 1.326 ± 0.385
3.609GlyTyr: 3.609 ± 0.536
0.0GlyXaa: 0.0 ± 0.0
His
1.105HisAla: 1.105 ± 0.26
0.516HisCys: 0.516 ± 0.2
1.031HisAsp: 1.031 ± 0.264
1.621HisGlu: 1.621 ± 0.369
0.368HisPhe: 0.368 ± 0.16
1.621HisGly: 1.621 ± 0.349
0.295HisHis: 0.295 ± 0.139
1.326HisIle: 1.326 ± 0.441
1.105HisLys: 1.105 ± 0.256
1.326HisLeu: 1.326 ± 0.264
0.442HisMet: 0.442 ± 0.181
0.884HisAsn: 0.884 ± 0.247
0.81HisPro: 0.81 ± 0.263
0.663HisGln: 0.663 ± 0.216
0.884HisArg: 0.884 ± 0.256
0.884HisSer: 0.884 ± 0.21
0.516HisThr: 0.516 ± 0.221
1.105HisVal: 1.105 ± 0.363
0.221HisTrp: 0.221 ± 0.124
0.663HisTyr: 0.663 ± 0.198
0.0HisXaa: 0.0 ± 0.0
Ile
6.04IleAla: 6.04 ± 0.604
1.473IleCys: 1.473 ± 0.406
5.156IleAsp: 5.156 ± 0.62
4.272IleGlu: 4.272 ± 0.628
2.652IlePhe: 2.652 ± 0.395
3.978IleGly: 3.978 ± 0.632
1.179IleHis: 1.179 ± 0.253
4.935IleIle: 4.935 ± 0.706
3.904IleLys: 3.904 ± 0.463
4.346IleLeu: 4.346 ± 0.564
1.915IleMet: 1.915 ± 0.445
3.757IleAsn: 3.757 ± 0.483
2.431IlePro: 2.431 ± 0.407
1.547IleGln: 1.547 ± 0.309
3.536IleArg: 3.536 ± 0.451
4.567IleSer: 4.567 ± 0.593
3.315IleThr: 3.315 ± 0.496
4.346IleVal: 4.346 ± 0.491
0.516IleTrp: 0.516 ± 0.16
1.621IleTyr: 1.621 ± 0.364
0.0IleXaa: 0.0 ± 0.0
Lys
6.556LysAla: 6.556 ± 0.785
1.326LysCys: 1.326 ± 0.409
2.283LysAsp: 2.283 ± 0.456
3.83LysGlu: 3.83 ± 0.488
2.136LysPhe: 2.136 ± 0.41
3.167LysGly: 3.167 ± 0.6
1.326LysHis: 1.326 ± 0.315
4.346LysIle: 4.346 ± 0.58
4.199LysLys: 4.199 ± 0.682
5.524LysLeu: 5.524 ± 0.608
2.799LysMet: 2.799 ± 0.507
4.346LysAsn: 4.346 ± 0.646
2.21LysPro: 2.21 ± 0.443
2.799LysGln: 2.799 ± 0.482
3.094LysArg: 3.094 ± 0.677
5.156LysSer: 5.156 ± 0.724
3.02LysThr: 3.02 ± 0.396
3.683LysVal: 3.683 ± 0.581
1.252LysTrp: 1.252 ± 0.335
2.357LysTyr: 2.357 ± 0.442
0.0LysXaa: 0.0 ± 0.0
Leu
6.04LeuAla: 6.04 ± 0.864
1.105LeuCys: 1.105 ± 0.264
3.094LeuAsp: 3.094 ± 0.475
4.272LeuGlu: 4.272 ± 0.556
1.547LeuPhe: 1.547 ± 0.289
3.757LeuGly: 3.757 ± 0.507
1.252LeuHis: 1.252 ± 0.271
4.346LeuIle: 4.346 ± 0.563
5.303LeuLys: 5.303 ± 0.749
4.788LeuLeu: 4.788 ± 0.655
1.694LeuMet: 1.694 ± 0.295
3.904LeuAsn: 3.904 ± 0.486
3.683LeuPro: 3.683 ± 0.532
2.946LeuGln: 2.946 ± 0.57
4.714LeuArg: 4.714 ± 0.631
4.862LeuSer: 4.862 ± 0.67
4.935LeuThr: 4.935 ± 0.565
4.199LeuVal: 4.199 ± 0.666
1.547LeuTrp: 1.547 ± 0.293
2.578LeuTyr: 2.578 ± 0.554
0.0LeuXaa: 0.0 ± 0.0
Met
3.315MetAla: 3.315 ± 0.468
0.368MetCys: 0.368 ± 0.189
1.915MetAsp: 1.915 ± 0.349
1.547MetGlu: 1.547 ± 0.287
1.694MetPhe: 1.694 ± 0.422
1.326MetGly: 1.326 ± 0.306
0.81MetHis: 0.81 ± 0.264
2.062MetIle: 2.062 ± 0.438
2.578MetLys: 2.578 ± 0.422
1.915MetLeu: 1.915 ± 0.369
1.326MetMet: 1.326 ± 0.292
1.841MetAsn: 1.841 ± 0.397
1.694MetPro: 1.694 ± 0.476
1.252MetGln: 1.252 ± 0.311
1.547MetArg: 1.547 ± 0.307
2.136MetSer: 2.136 ± 0.415
2.062MetThr: 2.062 ± 0.422
1.547MetVal: 1.547 ± 0.42
0.589MetTrp: 0.589 ± 0.2
0.958MetTyr: 0.958 ± 0.25
0.0MetXaa: 0.0 ± 0.0
Asn
5.377AsnAla: 5.377 ± 0.749
0.663AsnCys: 0.663 ± 0.209
3.315AsnAsp: 3.315 ± 0.398
2.873AsnGlu: 2.873 ± 0.37
1.473AsnPhe: 1.473 ± 0.42
4.493AsnGly: 4.493 ± 0.598
1.031AsnHis: 1.031 ± 0.389
2.799AsnIle: 2.799 ± 0.467
3.094AsnLys: 3.094 ± 0.641
3.388AsnLeu: 3.388 ± 0.537
1.179AsnMet: 1.179 ± 0.293
1.915AsnAsn: 1.915 ± 0.384
2.283AsnPro: 2.283 ± 0.427
1.989AsnGln: 1.989 ± 0.348
2.21AsnArg: 2.21 ± 0.427
2.946AsnSer: 2.946 ± 0.548
2.21AsnThr: 2.21 ± 0.437
3.388AsnVal: 3.388 ± 0.652
0.737AsnTrp: 0.737 ± 0.255
1.547AsnTyr: 1.547 ± 0.35
0.0AsnXaa: 0.0 ± 0.0
Pro
3.388ProAla: 3.388 ± 0.509
0.589ProCys: 0.589 ± 0.251
2.873ProAsp: 2.873 ± 0.486
2.652ProGlu: 2.652 ± 0.444
1.179ProPhe: 1.179 ± 0.305
2.136ProGly: 2.136 ± 0.385
0.81ProHis: 0.81 ± 0.242
2.21ProIle: 2.21 ± 0.332
1.694ProLys: 1.694 ± 0.414
2.062ProLeu: 2.062 ± 0.397
0.958ProMet: 0.958 ± 0.267
1.031ProAsn: 1.031 ± 0.292
1.031ProPro: 1.031 ± 0.275
0.958ProGln: 0.958 ± 0.335
1.326ProArg: 1.326 ± 0.378
2.578ProSer: 2.578 ± 0.45
1.473ProThr: 1.473 ± 0.308
2.946ProVal: 2.946 ± 0.565
0.442ProTrp: 0.442 ± 0.186
1.326ProTyr: 1.326 ± 0.32
0.0ProXaa: 0.0 ± 0.0
Gln
3.094GlnAla: 3.094 ± 0.75
0.368GlnCys: 0.368 ± 0.165
1.473GlnAsp: 1.473 ± 0.361
3.094GlnGlu: 3.094 ± 0.475
1.4GlnPhe: 1.4 ± 0.429
1.473GlnGly: 1.473 ± 0.398
1.326GlnHis: 1.326 ± 0.341
2.652GlnIle: 2.652 ± 0.48
2.357GlnLys: 2.357 ± 0.465
3.462GlnLeu: 3.462 ± 0.624
1.547GlnMet: 1.547 ± 0.357
1.4GlnAsn: 1.4 ± 0.299
1.621GlnPro: 1.621 ± 0.371
3.536GlnGln: 3.536 ± 1.105
1.989GlnArg: 1.989 ± 0.453
2.652GlnSer: 2.652 ± 0.443
2.431GlnThr: 2.431 ± 0.429
2.21GlnVal: 2.21 ± 0.412
0.737GlnTrp: 0.737 ± 0.192
1.473GlnTyr: 1.473 ± 0.306
0.0GlnXaa: 0.0 ± 0.0
Arg
4.346ArgAla: 4.346 ± 0.479
1.031ArgCys: 1.031 ± 0.358
2.725ArgAsp: 2.725 ± 0.514
3.904ArgGlu: 3.904 ± 0.497
2.062ArgPhe: 2.062 ± 0.483
2.873ArgGly: 2.873 ± 0.507
0.737ArgHis: 0.737 ± 0.186
3.094ArgIle: 3.094 ± 0.446
3.536ArgLys: 3.536 ± 0.644
4.567ArgLeu: 4.567 ± 0.538
1.179ArgMet: 1.179 ± 0.254
3.683ArgAsn: 3.683 ± 0.49
1.252ArgPro: 1.252 ± 0.324
1.841ArgGln: 1.841 ± 0.405
2.578ArgArg: 2.578 ± 0.436
2.578ArgSer: 2.578 ± 0.395
2.283ArgThr: 2.283 ± 0.488
3.02ArgVal: 3.02 ± 0.457
0.737ArgTrp: 0.737 ± 0.239
3.094ArgTyr: 3.094 ± 0.484
0.0ArgXaa: 0.0 ± 0.0
Ser
6.556SerAla: 6.556 ± 1.056
0.589SerCys: 0.589 ± 0.206
4.493SerAsp: 4.493 ± 0.495
3.978SerGlu: 3.978 ± 0.541
2.799SerPhe: 2.799 ± 0.492
5.893SerGly: 5.893 ± 0.61
0.737SerHis: 0.737 ± 0.195
3.241SerIle: 3.241 ± 0.513
3.978SerLys: 3.978 ± 0.649
4.714SerLeu: 4.714 ± 0.538
2.431SerMet: 2.431 ± 0.401
2.799SerAsn: 2.799 ± 0.439
1.989SerPro: 1.989 ± 0.388
2.725SerGln: 2.725 ± 0.487
2.357SerArg: 2.357 ± 0.474
4.051SerSer: 4.051 ± 0.671
3.904SerThr: 3.904 ± 0.675
3.536SerVal: 3.536 ± 0.511
0.81SerTrp: 0.81 ± 0.251
2.431SerTyr: 2.431 ± 0.314
0.0SerXaa: 0.0 ± 0.0
Thr
3.904ThrAla: 3.904 ± 0.487
0.589ThrCys: 0.589 ± 0.19
3.462ThrAsp: 3.462 ± 0.522
4.567ThrGlu: 4.567 ± 0.552
1.621ThrPhe: 1.621 ± 0.39
5.377ThrGly: 5.377 ± 0.698
0.884ThrHis: 0.884 ± 0.243
3.609ThrIle: 3.609 ± 0.515
3.462ThrLys: 3.462 ± 0.435
3.02ThrLeu: 3.02 ± 0.438
1.326ThrMet: 1.326 ± 0.289
2.504ThrAsn: 2.504 ± 0.55
2.799ThrPro: 2.799 ± 0.492
2.062ThrGln: 2.062 ± 0.548
2.357ThrArg: 2.357 ± 0.457
3.978ThrSer: 3.978 ± 0.562
3.462ThrThr: 3.462 ± 0.61
2.873ThrVal: 2.873 ± 0.521
0.589ThrTrp: 0.589 ± 0.322
1.841ThrTyr: 1.841 ± 0.398
0.0ThrXaa: 0.0 ± 0.0
Val
5.524ValAla: 5.524 ± 0.812
0.221ValCys: 0.221 ± 0.132
3.904ValAsp: 3.904 ± 0.617
3.683ValGlu: 3.683 ± 0.679
1.915ValPhe: 1.915 ± 0.41
3.167ValGly: 3.167 ± 0.528
0.663ValHis: 0.663 ± 0.233
5.303ValIle: 5.303 ± 0.525
4.051ValLys: 4.051 ± 0.501
4.567ValLeu: 4.567 ± 0.718
2.136ValMet: 2.136 ± 0.35
2.652ValAsn: 2.652 ± 0.504
1.547ValPro: 1.547 ± 0.363
2.21ValGln: 2.21 ± 0.337
2.799ValArg: 2.799 ± 0.427
5.23ValSer: 5.23 ± 0.667
4.346ValThr: 4.346 ± 0.744
4.714ValVal: 4.714 ± 0.679
1.031ValTrp: 1.031 ± 0.281
2.873ValTyr: 2.873 ± 0.403
0.0ValXaa: 0.0 ± 0.0
Trp
1.031TrpAla: 1.031 ± 0.288
0.589TrpCys: 0.589 ± 0.189
1.179TrpAsp: 1.179 ± 0.339
0.81TrpGlu: 0.81 ± 0.28
0.81TrpPhe: 0.81 ± 0.26
0.516TrpGly: 0.516 ± 0.202
0.663TrpHis: 0.663 ± 0.211
0.81TrpIle: 0.81 ± 0.271
1.326TrpLys: 1.326 ± 0.31
1.179TrpLeu: 1.179 ± 0.395
0.295TrpMet: 0.295 ± 0.159
0.663TrpAsn: 0.663 ± 0.257
0.589TrpPro: 0.589 ± 0.189
1.031TrpGln: 1.031 ± 0.256
1.252TrpArg: 1.252 ± 0.316
0.737TrpSer: 0.737 ± 0.215
0.81TrpThr: 0.81 ± 0.235
1.4TrpVal: 1.4 ± 0.322
0.295TrpTrp: 0.295 ± 0.15
0.295TrpTyr: 0.295 ± 0.128
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.536TyrAla: 3.536 ± 0.395
0.442TyrCys: 0.442 ± 0.152
3.315TyrAsp: 3.315 ± 0.417
2.283TyrGlu: 2.283 ± 0.442
1.841TyrPhe: 1.841 ± 0.423
2.504TyrGly: 2.504 ± 0.43
0.958TyrHis: 0.958 ± 0.24
2.136TyrIle: 2.136 ± 0.471
2.136TyrLys: 2.136 ± 0.406
2.357TyrLeu: 2.357 ± 0.552
0.516TyrMet: 0.516 ± 0.183
1.547TyrAsn: 1.547 ± 0.288
1.547TyrPro: 1.547 ± 0.43
1.473TyrGln: 1.473 ± 0.303
2.283TyrArg: 2.283 ± 0.369
2.578TyrSer: 2.578 ± 0.369
2.431TyrThr: 2.431 ± 0.569
2.578TyrVal: 2.578 ± 0.42
0.884TyrTrp: 0.884 ± 0.246
1.4TyrTyr: 1.4 ± 0.356
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 78 proteins (13577 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski