Amino acid dipepetide frequency for Microbacterium sp. CH12i

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.757AlaAla: 18.757 ± 0.244
0.677AlaCys: 0.677 ± 0.034
7.965AlaAsp: 7.965 ± 0.103
8.096AlaGlu: 8.096 ± 0.116
3.849AlaPhe: 3.849 ± 0.073
11.083AlaGly: 11.083 ± 0.128
2.52AlaHis: 2.52 ± 0.069
6.375AlaIle: 6.375 ± 0.093
2.978AlaLys: 2.978 ± 0.071
13.527AlaLeu: 13.527 ± 0.154
2.846AlaMet: 2.846 ± 0.058
2.505AlaAsn: 2.505 ± 0.059
5.96AlaPro: 5.96 ± 0.116
3.979AlaGln: 3.979 ± 0.076
8.42AlaArg: 8.42 ± 0.129
7.588AlaSer: 7.588 ± 0.1
7.345AlaThr: 7.345 ± 0.109
10.656AlaVal: 10.656 ± 0.13
1.692AlaTrp: 1.692 ± 0.05
2.293AlaTyr: 2.293 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
0.712CysAla: 0.712 ± 0.034
0.062CysCys: 0.062 ± 0.009
0.323CysAsp: 0.323 ± 0.022
0.274CysGlu: 0.274 ± 0.019
0.191CysPhe: 0.191 ± 0.016
0.599CysGly: 0.599 ± 0.033
0.135CysHis: 0.135 ± 0.013
0.209CysIle: 0.209 ± 0.016
0.07CysLys: 0.07 ± 0.009
0.463CysLeu: 0.463 ± 0.027
0.098CysMet: 0.098 ± 0.013
0.126CysAsn: 0.126 ± 0.013
0.302CysPro: 0.302 ± 0.023
0.112CysGln: 0.112 ± 0.013
0.348CysArg: 0.348 ± 0.023
0.325CysSer: 0.325 ± 0.021
0.333CysThr: 0.333 ± 0.02
0.465CysVal: 0.465 ± 0.024
0.078CysTrp: 0.078 ± 0.009
0.116CysTyr: 0.116 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
9.132AspAla: 9.132 ± 0.117
0.238AspCys: 0.238 ± 0.016
4.296AspAsp: 4.296 ± 0.104
4.449AspGlu: 4.449 ± 0.081
1.752AspPhe: 1.752 ± 0.054
5.643AspGly: 5.643 ± 0.083
1.196AspHis: 1.196 ± 0.042
2.821AspIle: 2.821 ± 0.064
1.19AspLys: 1.19 ± 0.041
6.03AspLeu: 6.03 ± 0.105
0.936AspMet: 0.936 ± 0.039
1.159AspAsn: 1.159 ± 0.049
3.699AspPro: 3.699 ± 0.062
1.688AspGln: 1.688 ± 0.052
4.201AspArg: 4.201 ± 0.078
2.993AspSer: 2.993 ± 0.062
3.066AspThr: 3.066 ± 0.068
5.305AspVal: 5.305 ± 0.081
0.912AspTrp: 0.912 ± 0.033
1.211AspTyr: 1.211 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
6.668GluAla: 6.668 ± 0.098
0.242GluCys: 0.242 ± 0.017
2.91GluAsp: 2.91 ± 0.071
3.089GluGlu: 3.089 ± 0.074
1.913GluPhe: 1.913 ± 0.053
4.046GluGly: 4.046 ± 0.075
1.591GluHis: 1.591 ± 0.043
3.202GluIle: 3.202 ± 0.073
1.686GluLys: 1.686 ± 0.053
6.528GluLeu: 6.528 ± 0.104
1.107GluMet: 1.107 ± 0.044
1.458GluAsn: 1.458 ± 0.045
2.795GluPro: 2.795 ± 0.061
2.362GluGln: 2.362 ± 0.058
5.124GluArg: 5.124 ± 0.088
3.317GluSer: 3.317 ± 0.063
3.15GluThr: 3.15 ± 0.063
4.625GluVal: 4.625 ± 0.078
0.936GluTrp: 0.936 ± 0.037
1.233GluTyr: 1.233 ± 0.041
0.0GluXaa: 0.0 ± 0.0
Phe
4.258PheAla: 4.258 ± 0.084
0.19PheCys: 0.19 ± 0.017
2.293PheAsp: 2.293 ± 0.061
1.9PheGlu: 1.9 ± 0.052
1.129PhePhe: 1.129 ± 0.038
3.353PheGly: 3.353 ± 0.073
0.579PheHis: 0.579 ± 0.03
1.502PheIle: 1.502 ± 0.048
0.525PheLys: 0.525 ± 0.024
2.803PheLeu: 2.803 ± 0.058
0.505PheMet: 0.505 ± 0.024
0.755PheAsn: 0.755 ± 0.03
1.404PhePro: 1.404 ± 0.046
0.823PheGln: 0.823 ± 0.034
1.788PheArg: 1.788 ± 0.048
1.769PheSer: 1.769 ± 0.051
2.265PheThr: 2.265 ± 0.051
2.721PheVal: 2.721 ± 0.071
0.458PheTrp: 0.458 ± 0.027
0.644PheTyr: 0.644 ± 0.033
0.0PheXaa: 0.0 ± 0.0
Gly
10.098GlyAla: 10.098 ± 0.138
0.576GlyCys: 0.576 ± 0.028
4.845GlyAsp: 4.845 ± 0.086
4.924GlyGlu: 4.924 ± 0.079
3.1GlyPhe: 3.1 ± 0.062
7.052GlyGly: 7.052 ± 0.104
1.825GlyHis: 1.825 ± 0.057
5.258GlyIle: 5.258 ± 0.085
2.45GlyLys: 2.45 ± 0.062
8.2GlyLeu: 8.2 ± 0.106
1.956GlyMet: 1.956 ± 0.054
1.903GlyAsn: 1.903 ± 0.059
3.254GlyPro: 3.254 ± 0.085
2.372GlyGln: 2.372 ± 0.058
5.746GlyArg: 5.746 ± 0.096
5.232GlySer: 5.232 ± 0.091
5.33GlyThr: 5.33 ± 0.088
7.389GlyVal: 7.389 ± 0.099
1.634GlyTrp: 1.634 ± 0.044
2.243GlyTyr: 2.243 ± 0.058
0.0GlyXaa: 0.0 ± 0.0
His
2.373HisAla: 2.373 ± 0.063
0.119HisCys: 0.119 ± 0.013
1.401HisAsp: 1.401 ± 0.044
1.236HisGlu: 1.236 ± 0.039
0.608HisPhe: 0.608 ± 0.027
1.924HisGly: 1.924 ± 0.057
0.594HisHis: 0.594 ± 0.03
0.904HisIle: 0.904 ± 0.035
0.34HisLys: 0.34 ± 0.019
2.095HisLeu: 2.095 ± 0.057
0.38HisMet: 0.38 ± 0.024
0.406HisAsn: 0.406 ± 0.023
1.432HisPro: 1.432 ± 0.044
0.551HisGln: 0.551 ± 0.028
1.65HisArg: 1.65 ± 0.048
1.107HisSer: 1.107 ± 0.039
1.071HisThr: 1.071 ± 0.042
1.685HisVal: 1.685 ± 0.044
0.293HisTrp: 0.293 ± 0.02
0.43HisTyr: 0.43 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
7.686IleAla: 7.686 ± 0.108
0.321IleCys: 0.321 ± 0.021
3.879IleAsp: 3.879 ± 0.062
3.294IleGlu: 3.294 ± 0.059
1.356IlePhe: 1.356 ± 0.049
5.077IleGly: 5.077 ± 0.095
0.801IleHis: 0.801 ± 0.036
2.549IleIle: 2.549 ± 0.067
0.945IleLys: 0.945 ± 0.041
4.29IleLeu: 4.29 ± 0.084
0.756IleMet: 0.756 ± 0.033
1.104IleAsn: 1.104 ± 0.04
2.578IlePro: 2.578 ± 0.057
1.197IleGln: 1.197 ± 0.036
3.05IleArg: 3.05 ± 0.065
2.922IleSer: 2.922 ± 0.063
3.379IleThr: 3.379 ± 0.069
5.096IleVal: 5.096 ± 0.079
0.636IleTrp: 0.636 ± 0.03
0.857IleTyr: 0.857 ± 0.036
0.0IleXaa: 0.0 ± 0.0
Lys
2.812LysAla: 2.812 ± 0.063
0.073LysCys: 0.073 ± 0.009
1.334LysAsp: 1.334 ± 0.041
1.103LysGlu: 1.103 ± 0.038
0.559LysPhe: 0.559 ± 0.026
1.732LysGly: 1.732 ± 0.05
0.534LysHis: 0.534 ± 0.029
1.234LysIle: 1.234 ± 0.04
0.931LysLys: 0.931 ± 0.043
2.084LysLeu: 2.084 ± 0.053
0.479LysMet: 0.479 ± 0.025
0.613LysAsn: 0.613 ± 0.028
1.354LysPro: 1.354 ± 0.048
0.794LysGln: 0.794 ± 0.032
1.773LysArg: 1.773 ± 0.056
1.352LysSer: 1.352 ± 0.049
1.549LysThr: 1.549 ± 0.049
1.961LysVal: 1.961 ± 0.055
0.328LysTrp: 0.328 ± 0.021
0.524LysTyr: 0.524 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
13.607LeuAla: 13.607 ± 0.146
0.553LeuCys: 0.553 ± 0.026
6.482LeuAsp: 6.482 ± 0.097
5.185LeuGlu: 5.185 ± 0.095
3.057LeuPhe: 3.057 ± 0.07
8.805LeuGly: 8.805 ± 0.118
1.912LeuHis: 1.912 ± 0.051
5.23LeuIle: 5.23 ± 0.113
2.034LeuLys: 2.034 ± 0.051
10.059LeuLeu: 10.059 ± 0.148
1.798LeuMet: 1.798 ± 0.056
2.153LeuAsn: 2.153 ± 0.058
5.13LeuPro: 5.13 ± 0.084
2.641LeuGln: 2.641 ± 0.058
7.227LeuArg: 7.227 ± 0.109
6.176LeuSer: 6.176 ± 0.09
6.312LeuThr: 6.312 ± 0.094
8.585LeuVal: 8.585 ± 0.107
1.26LeuTrp: 1.26 ± 0.042
1.618LeuTyr: 1.618 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
2.193MetAla: 2.193 ± 0.061
0.105MetCys: 0.105 ± 0.013
0.879MetAsp: 0.879 ± 0.033
0.678MetGlu: 0.678 ± 0.034
0.573MetPhe: 0.573 ± 0.028
1.261MetGly: 1.261 ± 0.045
0.389MetHis: 0.389 ± 0.021
1.051MetIle: 1.051 ± 0.04
0.532MetLys: 0.532 ± 0.027
2.215MetLeu: 2.215 ± 0.059
0.441MetMet: 0.441 ± 0.03
0.684MetAsn: 0.684 ± 0.031
1.24MetPro: 1.24 ± 0.041
0.615MetGln: 0.615 ± 0.035
1.576MetArg: 1.576 ± 0.04
1.649MetSer: 1.649 ± 0.042
1.859MetThr: 1.859 ± 0.049
1.394MetVal: 1.394 ± 0.039
0.228MetTrp: 0.228 ± 0.016
0.32MetTyr: 0.32 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
2.886AsnAla: 2.886 ± 0.065
0.133AsnCys: 0.133 ± 0.015
1.384AsnAsp: 1.384 ± 0.045
1.203AsnGlu: 1.203 ± 0.037
0.627AsnPhe: 0.627 ± 0.028
2.057AsnGly: 2.057 ± 0.048
0.425AsnHis: 0.425 ± 0.021
1.081AsnIle: 1.081 ± 0.038
0.53AsnLys: 0.53 ± 0.028
2.144AsnLeu: 2.144 ± 0.048
0.377AsnMet: 0.377 ± 0.021
0.551AsnAsn: 0.551 ± 0.029
1.642AsnPro: 1.642 ± 0.047
0.602AsnGln: 0.602 ± 0.028
1.413AsnArg: 1.413 ± 0.043
1.256AsnSer: 1.256 ± 0.048
1.302AsnThr: 1.302 ± 0.042
1.794AsnVal: 1.794 ± 0.045
0.38AsnTrp: 0.38 ± 0.024
0.523AsnTyr: 0.523 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
6.263ProAla: 6.263 ± 0.119
0.196ProCys: 0.196 ± 0.015
3.576ProAsp: 3.576 ± 0.064
3.69ProGlu: 3.69 ± 0.064
1.776ProPhe: 1.776 ± 0.05
4.332ProGly: 4.332 ± 0.085
1.127ProHis: 1.127 ± 0.04
2.33ProIle: 2.33 ± 0.057
1.164ProLys: 1.164 ± 0.037
4.795ProLeu: 4.795 ± 0.074
0.947ProMet: 0.947 ± 0.037
1.171ProAsn: 1.171 ± 0.04
2.053ProPro: 2.053 ± 0.078
1.532ProGln: 1.532 ± 0.052
3.095ProArg: 3.095 ± 0.072
3.1ProSer: 3.1 ± 0.06
3.282ProThr: 3.282 ± 0.079
4.348ProVal: 4.348 ± 0.07
0.779ProTrp: 0.779 ± 0.035
0.99ProTyr: 0.99 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
3.493GlnAla: 3.493 ± 0.071
0.144GlnCys: 0.144 ± 0.014
1.348GlnAsp: 1.348 ± 0.041
1.375GlnGlu: 1.375 ± 0.047
0.888GlnPhe: 0.888 ± 0.029
2.125GlnGly: 2.125 ± 0.05
0.74GlnHis: 0.74 ± 0.031
1.673GlnIle: 1.673 ± 0.044
0.788GlnLys: 0.788 ± 0.032
3.288GlnLeu: 3.288 ± 0.07
0.621GlnMet: 0.621 ± 0.032
0.8GlnAsn: 0.8 ± 0.032
1.435GlnPro: 1.435 ± 0.045
1.325GlnGln: 1.325 ± 0.05
2.572GlnArg: 2.572 ± 0.07
1.701GlnSer: 1.701 ± 0.05
1.655GlnThr: 1.655 ± 0.044
2.46GlnVal: 2.46 ± 0.062
0.465GlnTrp: 0.465 ± 0.024
0.606GlnTyr: 0.606 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
8.353ArgAla: 8.353 ± 0.104
0.335ArgCys: 0.335 ± 0.027
4.092ArgAsp: 4.092 ± 0.08
4.347ArgGlu: 4.347 ± 0.085
2.323ArgPhe: 2.323 ± 0.05
5.2ArgGly: 5.2 ± 0.093
1.548ArgHis: 1.548 ± 0.04
3.679ArgIle: 3.679 ± 0.064
1.715ArgLys: 1.715 ± 0.048
6.778ArgLeu: 6.778 ± 0.093
1.845ArgMet: 1.845 ± 0.046
1.465ArgAsn: 1.465 ± 0.045
3.256ArgPro: 3.256 ± 0.072
2.181ArgGln: 2.181 ± 0.053
6.127ArgArg: 6.127 ± 0.112
4.15ArgSer: 4.15 ± 0.091
4.355ArgThr: 4.355 ± 0.08
5.737ArgVal: 5.737 ± 0.093
1.164ArgTrp: 1.164 ± 0.041
1.534ArgTyr: 1.534 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
7.177SerAla: 7.177 ± 0.104
0.305SerCys: 0.305 ± 0.022
3.555SerAsp: 3.555 ± 0.068
3.202SerGlu: 3.202 ± 0.07
1.965SerPhe: 1.965 ± 0.057
5.741SerGly: 5.741 ± 0.097
1.088SerHis: 1.088 ± 0.036
2.985SerIle: 2.985 ± 0.065
1.348SerLys: 1.348 ± 0.038
5.654SerLeu: 5.654 ± 0.076
1.426SerMet: 1.426 ± 0.041
1.338SerAsn: 1.338 ± 0.047
3.021SerPro: 3.021 ± 0.068
1.562SerGln: 1.562 ± 0.048
3.916SerArg: 3.916 ± 0.071
3.703SerSer: 3.703 ± 0.076
3.949SerThr: 3.949 ± 0.078
4.957SerVal: 4.957 ± 0.08
0.986SerTrp: 0.986 ± 0.038
1.19SerTyr: 1.19 ± 0.044
0.0SerXaa: 0.0 ± 0.0
Thr
7.687ThrAla: 7.687 ± 0.104
0.324ThrCys: 0.324 ± 0.02
3.84ThrAsp: 3.84 ± 0.083
3.299ThrGlu: 3.299 ± 0.067
1.876ThrPhe: 1.876 ± 0.046
5.611ThrGly: 5.611 ± 0.093
1.187ThrHis: 1.187 ± 0.04
3.259ThrIle: 3.259 ± 0.067
1.424ThrLys: 1.424 ± 0.046
6.187ThrLeu: 6.187 ± 0.091
1.152ThrMet: 1.152 ± 0.038
1.308ThrAsn: 1.308 ± 0.033
3.982ThrPro: 3.982 ± 0.083
1.706ThrGln: 1.706 ± 0.051
3.703ThrArg: 3.703 ± 0.074
3.628ThrSer: 3.628 ± 0.065
3.979ThrThr: 3.979 ± 0.093
5.588ThrVal: 5.588 ± 0.098
0.872ThrTrp: 0.872 ± 0.036
1.162ThrTyr: 1.162 ± 0.047
0.0ThrXaa: 0.0 ± 0.0
Val
10.929ValAla: 10.929 ± 0.121
0.541ValCys: 0.541 ± 0.031
5.466ValAsp: 5.466 ± 0.089
4.832ValGlu: 4.832 ± 0.078
2.853ValPhe: 2.853 ± 0.063
6.672ValGly: 6.672 ± 0.1
1.769ValHis: 1.769 ± 0.047
4.845ValIle: 4.845 ± 0.097
1.739ValLys: 1.739 ± 0.047
8.92ValLeu: 8.92 ± 0.118
1.523ValMet: 1.523 ± 0.047
1.892ValAsn: 1.892 ± 0.05
4.283ValPro: 4.283 ± 0.064
2.371ValGln: 2.371 ± 0.058
5.712ValArg: 5.712 ± 0.095
5.119ValSer: 5.119 ± 0.081
5.522ValThr: 5.522 ± 0.091
7.908ValVal: 7.908 ± 0.114
1.153ValTrp: 1.153 ± 0.036
1.387ValTyr: 1.387 ± 0.048
0.0ValXaa: 0.0 ± 0.0
Trp
1.686TrpAla: 1.686 ± 0.045
0.091TrpCys: 0.091 ± 0.011
0.791TrpAsp: 0.791 ± 0.035
0.687TrpGlu: 0.687 ± 0.03
0.573TrpPhe: 0.573 ± 0.033
1.148TrpGly: 1.148 ± 0.044
0.344TrpHis: 0.344 ± 0.019
0.756TrpIle: 0.756 ± 0.033
0.321TrpLys: 0.321 ± 0.021
1.704TrpLeu: 1.704 ± 0.042
0.38TrpMet: 0.38 ± 0.022
0.449TrpAsn: 0.449 ± 0.023
0.704TrpPro: 0.704 ± 0.032
0.479TrpGln: 0.479 ± 0.024
1.217TrpArg: 1.217 ± 0.044
0.871TrpSer: 0.871 ± 0.031
0.904TrpThr: 0.904 ± 0.034
1.191TrpVal: 1.191 ± 0.04
0.388TrpTrp: 0.388 ± 0.027
0.274TrpTyr: 0.274 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.343TyrAla: 2.343 ± 0.057
0.117TyrCys: 0.117 ± 0.014
1.261TyrAsp: 1.261 ± 0.043
1.118TyrGlu: 1.118 ± 0.043
0.696TyrPhe: 0.696 ± 0.031
1.926TyrGly: 1.926 ± 0.046
0.296TyrHis: 0.296 ± 0.02
0.83TyrIle: 0.83 ± 0.034
0.412TyrLys: 0.412 ± 0.026
2.059TyrLeu: 2.059 ± 0.064
0.305TyrMet: 0.305 ± 0.02
0.472TyrAsn: 0.472 ± 0.027
1.021TyrPro: 1.021 ± 0.039
0.608TyrGln: 0.608 ± 0.029
1.528TyrArg: 1.528 ± 0.04
1.112TyrSer: 1.112 ± 0.038
1.167TyrThr: 1.167 ± 0.046
1.569TyrVal: 1.569 ± 0.048
0.315TyrTrp: 0.315 ± 0.021
0.468TyrTyr: 0.468 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2717 proteins (784182 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski