Amino acid dipepetide frequency for Microbispora rosea

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.452AlaAla: 21.452 ± 0.158	1.138AlaCys: 1.138 ± 0.02	7.866AlaAsp: 7.866 ± 0.063	8.5AlaGlu: 8.5 ± 0.069	3.644AlaPhe: 3.644 ± 0.039	13.821AlaGly: 13.821 ± 0.088	2.635AlaHis: 2.635 ± 0.032	4.042AlaIle: 4.042 ± 0.042	2.513AlaLys: 2.513 ± 0.035	14.4AlaLeu: 14.4 ± 0.095	2.71AlaMet: 2.71 ± 0.033	1.987AlaAsn: 1.987 ± 0.036	6.578AlaPro: 6.578 ± 0.067	3.388AlaGln: 3.388 ± 0.042	10.673AlaArg: 10.673 ± 0.085	6.027AlaSer: 6.027 ± 0.059	6.886AlaThr: 6.886 ± 0.056	12.358AlaVal: 12.358 ± 0.087	1.918AlaTrp: 1.918 ± 0.031	2.838AlaTyr: 2.838 ± 0.035	0.0AlaXaa: 0.0 ± 0.0
Cys	1.052CysAla: 1.052 ± 0.021	0.118CysCys: 0.118 ± 0.009	0.505CysAsp: 0.505 ± 0.016	0.413CysGlu: 0.413 ± 0.012	0.234CysPhe: 0.234 ± 0.011	0.956CysGly: 0.956 ± 0.02	0.212CysHis: 0.212 ± 0.009	0.141CysIle: 0.141 ± 0.008	0.095CysLys: 0.095 ± 0.006	0.801CysLeu: 0.801 ± 0.02	0.12CysMet: 0.12 ± 0.007	0.13CysAsn: 0.13 ± 0.009	0.512CysPro: 0.512 ± 0.015	0.175CysGln: 0.175 ± 0.009	0.643CysArg: 0.643 ± 0.017	0.444CysSer: 0.444 ± 0.015	0.426CysThr: 0.426 ± 0.012	0.679CysVal: 0.679 ± 0.015	0.126CysTrp: 0.126 ± 0.007	0.175CysTyr: 0.175 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.133AspAla: 7.133 ± 0.062	0.391AspCys: 0.391 ± 0.013	3.599AspAsp: 3.599 ± 0.045	3.687AspGlu: 3.687 ± 0.042	1.509AspPhe: 1.509 ± 0.026	6.132AspGly: 6.132 ± 0.055	1.355AspHis: 1.355 ± 0.026	1.799AspIle: 1.799 ± 0.03	1.054AspLys: 1.054 ± 0.025	6.642AspLeu: 6.642 ± 0.059	0.874AspMet: 0.874 ± 0.018	0.959AspAsn: 0.959 ± 0.023	4.478AspPro: 4.478 ± 0.04	1.455AspGln: 1.455 ± 0.022	4.873AspArg: 4.873 ± 0.052	2.202AspSer: 2.202 ± 0.033	2.658AspThr: 2.658 ± 0.039	5.087AspVal: 5.087 ± 0.044	0.966AspTrp: 0.966 ± 0.022	1.227AspTyr: 1.227 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	7.153GluAla: 7.153 ± 0.062	0.377GluCys: 0.377 ± 0.012	2.771GluAsp: 2.771 ± 0.04	3.526GluGlu: 3.526 ± 0.051	1.452GluPhe: 1.452 ± 0.023	4.154GluGly: 4.154 ± 0.046	1.505GluHis: 1.505 ± 0.025	2.595GluIle: 2.595 ± 0.037	1.126GluLys: 1.126 ± 0.022	6.144GluLeu: 6.144 ± 0.06	0.998GluMet: 0.998 ± 0.02	0.965GluAsn: 0.965 ± 0.019	3.333GluPro: 3.333 ± 0.039	1.997GluGln: 1.997 ± 0.029	5.684GluArg: 5.684 ± 0.061	2.51GluSer: 2.51 ± 0.032	2.914GluThr: 2.914 ± 0.037	4.662GluVal: 4.662 ± 0.047	0.866GluTrp: 0.866 ± 0.018	0.973GluTyr: 0.973 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	3.798PheAla: 3.798 ± 0.043	0.272PheCys: 0.272 ± 0.01	1.999PheAsp: 1.999 ± 0.029	1.431PheGlu: 1.431 ± 0.026	0.871PhePhe: 0.871 ± 0.02	3.094PheGly: 3.094 ± 0.041	0.614PheHis: 0.614 ± 0.016	0.74PheIle: 0.74 ± 0.02	0.459PheLys: 0.459 ± 0.012	2.645PheLeu: 2.645 ± 0.039	0.413PheMet: 0.413 ± 0.013	0.577PheAsn: 0.577 ± 0.015	1.429PhePro: 1.429 ± 0.025	0.667PheGln: 0.667 ± 0.016	1.887PheArg: 1.887 ± 0.026	1.445PheSer: 1.445 ± 0.024	2.011PheThr: 2.011 ± 0.029	2.342PheVal: 2.342 ± 0.032	0.437PheTrp: 0.437 ± 0.013	0.616PheTyr: 0.616 ± 0.014	0.0PheXaa: 0.0 ± 0.0
Gly	10.852GlyAla: 10.852 ± 0.072	0.822GlyCys: 0.822 ± 0.021	5.503GlyAsp: 5.503 ± 0.052	5.236GlyGlu: 5.236 ± 0.06	2.931GlyPhe: 2.931 ± 0.034	9.1GlyGly: 9.1 ± 0.09	2.301GlyHis: 2.301 ± 0.032	3.316GlyIle: 3.316 ± 0.039	2.189GlyLys: 2.189 ± 0.035	9.873GlyLeu: 9.873 ± 0.075	2.094GlyMet: 2.094 ± 0.035	1.78GlyAsn: 1.78 ± 0.035	5.31GlyPro: 5.31 ± 0.056	2.69GlyGln: 2.69 ± 0.04	8.318GlyArg: 8.318 ± 0.065	4.968GlySer: 4.968 ± 0.048	5.823GlyThr: 5.823 ± 0.059	8.192GlyVal: 8.192 ± 0.061	1.751GlyTrp: 1.751 ± 0.032	2.376GlyTyr: 2.376 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	2.631HisAla: 2.631 ± 0.035	0.209HisCys: 0.209 ± 0.009	1.355HisAsp: 1.355 ± 0.023	1.234HisGlu: 1.234 ± 0.021	0.591HisPhe: 0.591 ± 0.014	2.186HisGly: 2.186 ± 0.032	0.617HisHis: 0.617 ± 0.016	0.642HisIle: 0.642 ± 0.016	0.303HisLys: 0.303 ± 0.012	2.369HisLeu: 2.369 ± 0.034	0.347HisMet: 0.347 ± 0.012	0.375HisAsn: 0.375 ± 0.012	1.703HisPro: 1.703 ± 0.027	0.537HisGln: 0.537 ± 0.014	1.936HisArg: 1.936 ± 0.027	0.914HisSer: 0.914 ± 0.019	1.081HisThr: 1.081 ± 0.02	1.837HisVal: 1.837 ± 0.029	0.343HisTrp: 0.343 ± 0.012	0.491HisTyr: 0.491 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	5.166IleAla: 5.166 ± 0.049	0.293IleCys: 0.293 ± 0.01	2.357IleAsp: 2.357 ± 0.034	2.189IleGlu: 2.189 ± 0.034	0.806IlePhe: 0.806 ± 0.019	3.71IleGly: 3.71 ± 0.04	0.629IleHis: 0.629 ± 0.015	0.97IleIle: 0.97 ± 0.02	0.734IleLys: 0.734 ± 0.018	2.679IleLeu: 2.679 ± 0.038	0.568IleMet: 0.568 ± 0.015	0.704IleAsn: 0.704 ± 0.016	1.852IlePro: 1.852 ± 0.024	0.739IleGln: 0.739 ± 0.017	2.525IleArg: 2.525 ± 0.037	1.844IleSer: 1.844 ± 0.031	2.315IleThr: 2.315 ± 0.03	3.207IleVal: 3.207 ± 0.038	0.434IleTrp: 0.434 ± 0.014	0.597IleTyr: 0.597 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	2.546LysAla: 2.546 ± 0.035	0.122LysCys: 0.122 ± 0.008	1.103LysAsp: 1.103 ± 0.026	1.051LysGlu: 1.051 ± 0.023	0.407LysPhe: 0.407 ± 0.014	1.619LysGly: 1.619 ± 0.03	0.381LysHis: 0.381 ± 0.013	0.953LysIle: 0.953 ± 0.021	0.599LysLys: 0.599 ± 0.019	1.715LysLeu: 1.715 ± 0.027	0.355LysMet: 0.355 ± 0.012	0.44LysAsn: 0.44 ± 0.015	1.215LysPro: 1.215 ± 0.027	0.599LysGln: 0.599 ± 0.016	1.352LysArg: 1.352 ± 0.026	0.946LysSer: 0.946 ± 0.019	1.192LysThr: 1.192 ± 0.025	1.799LysVal: 1.799 ± 0.03	0.303LysTrp: 0.303 ± 0.012	0.409LysTyr: 0.409 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	15.562LeuAla: 15.562 ± 0.102	0.829LeuCys: 0.829 ± 0.019	6.578LeuAsp: 6.578 ± 0.06	4.707LeuGlu: 4.707 ± 0.045	2.634LeuPhe: 2.634 ± 0.037	9.427LeuGly: 9.427 ± 0.07	2.084LeuHis: 2.084 ± 0.032	3.454LeuIle: 3.454 ± 0.042	1.822LeuLys: 1.822 ± 0.031	11.302LeuLeu: 11.302 ± 0.097	1.716LeuMet: 1.716 ± 0.026	1.69LeuAsn: 1.69 ± 0.029	6.408LeuPro: 6.408 ± 0.056	2.003LeuGln: 2.003 ± 0.03	9.107LeuArg: 9.107 ± 0.075	5.486LeuSer: 5.486 ± 0.048	6.741LeuThr: 6.741 ± 0.047	9.174LeuVal: 9.174 ± 0.089	1.338LeuTrp: 1.338 ± 0.028	1.85LeuTyr: 1.85 ± 0.027	0.0LeuXaa: 0.0 ± 0.0
Met	2.383MetAla: 2.383 ± 0.03	0.132MetCys: 0.132 ± 0.008	0.923MetAsp: 0.923 ± 0.02	0.786MetGlu: 0.786 ± 0.018	0.489MetPhe: 0.489 ± 0.016	1.346MetGly: 1.346 ± 0.023	0.33MetHis: 0.33 ± 0.011	0.822MetIle: 0.822 ± 0.021	0.44MetLys: 0.44 ± 0.012	1.894MetLeu: 1.894 ± 0.028	0.304MetMet: 0.304 ± 0.01	0.459MetAsn: 0.459 ± 0.013	1.218MetPro: 1.218 ± 0.02	0.396MetGln: 0.396 ± 0.014	1.699MetArg: 1.699 ± 0.024	1.378MetSer: 1.378 ± 0.023	1.669MetThr: 1.669 ± 0.027	1.35MetVal: 1.35 ± 0.024	0.241MetTrp: 0.241 ± 0.009	0.313MetTyr: 0.313 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.272AsnAla: 2.272 ± 0.033	0.158AsnCys: 0.158 ± 0.007	0.923AsnAsp: 0.923 ± 0.02	0.773AsnGlu: 0.773 ± 0.018	0.482AsnPhe: 0.482 ± 0.016	2.052AsnGly: 2.052 ± 0.036	0.373AsnHis: 0.373 ± 0.013	0.621AsnIle: 0.621 ± 0.016	0.352AsnLys: 0.352 ± 0.013	1.815AsnLeu: 1.815 ± 0.031	0.284AsnMet: 0.284 ± 0.011	0.468AsnAsn: 0.468 ± 0.02	1.412AsnPro: 1.412 ± 0.026	0.528AsnGln: 0.528 ± 0.016	1.318AsnArg: 1.318 ± 0.022	0.849AsnSer: 0.849 ± 0.022	1.024AsnThr: 1.024 ± 0.028	1.498AsnVal: 1.498 ± 0.027	0.314AsnTrp: 0.314 ± 0.012	0.419AsnTyr: 0.419 ± 0.013	0.0AsnXaa: 0.0 ± 0.0
Pro	8.658ProAla: 8.658 ± 0.076	0.366ProCys: 0.366 ± 0.012	4.218ProAsp: 4.218 ± 0.043	4.177ProGlu: 4.177 ± 0.04	1.565ProPhe: 1.565 ± 0.025	7.077ProGly: 7.077 ± 0.063	1.294ProHis: 1.294 ± 0.023	1.751ProIle: 1.751 ± 0.031	1.116ProLys: 1.116 ± 0.025	5.293ProLeu: 5.293 ± 0.049	1.12ProMet: 1.12 ± 0.021	0.92ProAsn: 0.92 ± 0.024	3.912ProPro: 3.912 ± 0.058	1.534ProGln: 1.534 ± 0.03	4.086ProArg: 4.086 ± 0.043	3.616ProSer: 3.616 ± 0.056	2.786ProThr: 2.786 ± 0.039	5.414ProVal: 5.414 ± 0.053	0.931ProTrp: 0.931 ± 0.021	1.493ProTyr: 1.493 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	3.853GlnAla: 3.853 ± 0.037	0.148GlnCys: 0.148 ± 0.007	1.208GlnAsp: 1.208 ± 0.025	1.323GlnGlu: 1.323 ± 0.027	0.683GlnPhe: 0.683 ± 0.017	2.161GlnGly: 2.161 ± 0.029	0.53GlnHis: 0.53 ± 0.014	1.202GlnIle: 1.202 ± 0.022	0.525GlnLys: 0.525 ± 0.017	2.359GlnLeu: 2.359 ± 0.034	0.505GlnMet: 0.505 ± 0.014	0.493GlnAsn: 0.493 ± 0.014	1.55GlnPro: 1.55 ± 0.032	0.971GlnGln: 0.971 ± 0.032	2.2GlnArg: 2.2 ± 0.031	1.231GlnSer: 1.231 ± 0.022	1.346GlnThr: 1.346 ± 0.023	2.431GlnVal: 2.431 ± 0.036	0.476GlnTrp: 0.476 ± 0.014	0.468GlnTyr: 0.468 ± 0.014	0.0GlnXaa: 0.0 ± 0.0
Arg	10.115ArgAla: 10.115 ± 0.077	0.62ArgCys: 0.62 ± 0.015	4.613ArgAsp: 4.613 ± 0.05	5.055ArgGlu: 5.055 ± 0.054	2.542ArgPhe: 2.542 ± 0.031	6.018ArgGly: 6.018 ± 0.043	2.182ArgHis: 2.182 ± 0.033	3.265ArgIle: 3.265 ± 0.04	1.484ArgLys: 1.484 ± 0.022	9.843ArgLeu: 9.843 ± 0.083	1.943ArgMet: 1.943 ± 0.03	1.353ArgAsn: 1.353 ± 0.027	5.227ArgPro: 5.227 ± 0.047	2.401ArgGln: 2.401 ± 0.03	8.668ArgArg: 8.668 ± 0.08	4.002ArgSer: 4.002 ± 0.041	4.77ArgThr: 4.77 ± 0.044	6.46ArgVal: 6.46 ± 0.053	1.419ArgTrp: 1.419 ± 0.024	1.894ArgTyr: 1.894 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	6.536SerAla: 6.536 ± 0.058	0.402SerCys: 0.402 ± 0.013	2.546SerAsp: 2.546 ± 0.028	2.277SerGlu: 2.277 ± 0.029	1.51SerPhe: 1.51 ± 0.025	6.12SerGly: 6.12 ± 0.06	0.962SerHis: 0.962 ± 0.021	1.656SerIle: 1.656 ± 0.028	0.898SerLys: 0.898 ± 0.021	4.743SerLeu: 4.743 ± 0.049	1.105SerMet: 1.105 ± 0.021	0.885SerAsn: 0.885 ± 0.022	3.709SerPro: 3.709 ± 0.056	1.221SerGln: 1.221 ± 0.025	3.896SerArg: 3.896 ± 0.045	2.816SerSer: 2.816 ± 0.04	2.825SerThr: 2.825 ± 0.038	4.038SerVal: 4.038 ± 0.04	0.952SerTrp: 0.952 ± 0.019	1.225SerTyr: 1.225 ± 0.021	0.0SerXaa: 0.0 ± 0.0
Thr	8.147ThrAla: 8.147 ± 0.067	0.475ThrCys: 0.475 ± 0.014	2.86ThrAsp: 2.86 ± 0.034	2.654ThrGlu: 2.654 ± 0.036	1.706ThrPhe: 1.706 ± 0.026	6.525ThrGly: 6.525 ± 0.057	1.078ThrHis: 1.078 ± 0.021	2.05ThrIle: 2.05 ± 0.032	1.006ThrLys: 1.006 ± 0.022	5.679ThrLeu: 5.679 ± 0.044	1.045ThrMet: 1.045 ± 0.017	1.009ThrAsn: 1.009 ± 0.025	4.12ThrPro: 4.12 ± 0.044	1.3ThrGln: 1.3 ± 0.026	4.075ThrArg: 4.075 ± 0.046	3.084ThrSer: 3.084 ± 0.043	3.682ThrThr: 3.682 ± 0.044	5.662ThrVal: 5.662 ± 0.054	0.936ThrTrp: 0.936 ± 0.022	1.305ThrTyr: 1.305 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	11.714ValAla: 11.714 ± 0.083	0.752ValCys: 0.752 ± 0.017	4.845ValAsp: 4.845 ± 0.047	4.789ValGlu: 4.789 ± 0.047	2.526ValPhe: 2.526 ± 0.037	6.513ValGly: 6.513 ± 0.059	1.804ValHis: 1.804 ± 0.027	3.187ValIle: 3.187 ± 0.041	1.67ValLys: 1.67 ± 0.026	9.662ValLeu: 9.662 ± 0.086	1.489ValMet: 1.489 ± 0.025	1.816ValAsn: 1.816 ± 0.031	5.369ValPro: 5.369 ± 0.049	1.906ValGln: 1.906 ± 0.028	7.516ValArg: 7.516 ± 0.064	4.604ValSer: 4.604 ± 0.045	6.042ValThr: 6.042 ± 0.056	8.709ValVal: 8.709 ± 0.076	1.166ValTrp: 1.166 ± 0.019	1.657ValTyr: 1.657 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.676TrpAla: 1.676 ± 0.027	0.15TrpCys: 0.15 ± 0.007	0.851TrpAsp: 0.851 ± 0.02	0.681TrpGlu: 0.681 ± 0.018	0.519TrpPhe: 0.519 ± 0.014	1.064TrpGly: 1.064 ± 0.021	0.397TrpHis: 0.397 ± 0.011	0.585TrpIle: 0.585 ± 0.016	0.339TrpLys: 0.339 ± 0.013	1.847TrpLeu: 1.847 ± 0.026	0.312TrpMet: 0.312 ± 0.01	0.464TrpAsn: 0.464 ± 0.013	0.916TrpPro: 0.916 ± 0.021	0.551TrpGln: 0.551 ± 0.017	1.511TrpArg: 1.511 ± 0.027	0.97TrpSer: 0.97 ± 0.022	1.075TrpThr: 1.075 ± 0.023	0.997TrpVal: 0.997 ± 0.019	0.353TrpTrp: 0.353 ± 0.012	0.382TrpTyr: 0.382 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.672TyrAla: 2.672 ± 0.03	0.185TyrCys: 0.185 ± 0.009	1.454TyrAsp: 1.454 ± 0.03	1.213TyrGlu: 1.213 ± 0.023	0.652TyrPhe: 0.652 ± 0.015	2.35TyrGly: 2.35 ± 0.033	0.419TyrHis: 0.419 ± 0.014	0.527TyrIle: 0.527 ± 0.015	0.381TyrLys: 0.381 ± 0.013	2.209TyrLeu: 2.209 ± 0.028	0.27TyrMet: 0.27 ± 0.01	0.462TyrAsn: 0.462 ± 0.015	1.075TyrPro: 1.075 ± 0.021	0.604TyrGln: 0.604 ± 0.017	1.906TyrArg: 1.906 ± 0.031	0.987TyrSer: 0.987 ± 0.02	1.131TyrThr: 1.131 ± 0.02	1.84TyrVal: 1.84 ± 0.026	0.371TyrTrp: 0.371 ± 0.013	0.501TyrTyr: 0.501 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 8224 proteins (2616702 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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