Aestuariisphingobium litorale

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Aestuariisphingobium

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3475 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A419MXF9|A0A419MXF9_9SPHN Aldehyde dehydrogenase family protein OS=Aestuariisphingobium litorale OX=2339262 GN=D5I55_04315 PE=4 SV=1
MM1 pKa = 7.37TYY3 pKa = 10.66VVTEE7 pKa = 3.87ACIKK11 pKa = 9.69CKK13 pKa = 10.78YY14 pKa = 7.76MDD16 pKa = 4.28CVEE19 pKa = 4.32VCPVDD24 pKa = 3.79CFYY27 pKa = 11.19EE28 pKa = 4.58GEE30 pKa = 4.18NMLVINPNEE39 pKa = 4.6CIDD42 pKa = 4.23CGVCEE47 pKa = 4.44PEE49 pKa = 4.82CPAEE53 pKa = 5.13AILPDD58 pKa = 3.97TEE60 pKa = 4.55SGLEE64 pKa = 3.72KK65 pKa = 10.06WLEE68 pKa = 4.05LNSTYY73 pKa = 10.56SASWPNVTRR82 pKa = 11.84KK83 pKa = 10.1GDD85 pKa = 3.62QTPADD90 pKa = 4.07ADD92 pKa = 3.59DD93 pKa = 4.36HH94 pKa = 7.59KK95 pKa = 11.53GEE97 pKa = 3.94EE98 pKa = 4.29GKK100 pKa = 10.55YY101 pKa = 10.37DD102 pKa = 3.42KK103 pKa = 11.21YY104 pKa = 11.21FSAEE108 pKa = 3.96PGAGDD113 pKa = 3.38

Molecular weight: 12.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A419MVE4|A0A419MVE4_9SPHN Response regulator OS=Aestuariisphingobium litorale OX=2339262 GN=D5I55_12085 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3475

0

3475

1112920

27

7169

320.3

34.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.175 ± 0.061

0.789 ± 0.013

5.991 ± 0.034

5.292 ± 0.046

3.603 ± 0.029

8.85 ± 0.047

1.982 ± 0.022

5.023 ± 0.029

3.353 ± 0.036

9.711 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.538 ± 0.026

2.669 ± 0.033

5.204 ± 0.038

3.104 ± 0.021

6.936 ± 0.044

5.253 ± 0.046

5.51 ± 0.066

7.359 ± 0.031

1.444 ± 0.019

2.214 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski