Amino acid dipepetide frequency for Microbacterium trichothecenolyticum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.075AlaAla: 22.075 ± 0.211	0.671AlaCys: 0.671 ± 0.025	8.937AlaAsp: 8.937 ± 0.082	8.162AlaGlu: 8.162 ± 0.115	4.102AlaPhe: 4.102 ± 0.058	12.724AlaGly: 12.724 ± 0.128	2.529AlaHis: 2.529 ± 0.052	6.125AlaIle: 6.125 ± 0.073	2.556AlaLys: 2.556 ± 0.054	14.045AlaLeu: 14.045 ± 0.137	2.682AlaMet: 2.682 ± 0.045	2.3AlaAsn: 2.3 ± 0.043	7.052AlaPro: 7.052 ± 0.093	3.703AlaGln: 3.703 ± 0.058	9.475AlaArg: 9.475 ± 0.107	7.794AlaSer: 7.794 ± 0.089	7.913AlaThr: 7.913 ± 0.107	11.931AlaVal: 11.931 ± 0.116	2.144AlaTrp: 2.144 ± 0.046	2.562AlaTyr: 2.562 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.638CysAla: 0.638 ± 0.022	0.05CysCys: 0.05 ± 0.006	0.301CysAsp: 0.301 ± 0.014	0.264CysGlu: 0.264 ± 0.014	0.155CysPhe: 0.155 ± 0.011	0.52CysGly: 0.52 ± 0.022	0.114CysHis: 0.114 ± 0.01	0.172CysIle: 0.172 ± 0.012	0.053CysLys: 0.053 ± 0.007	0.428CysLeu: 0.428 ± 0.017	0.072CysMet: 0.072 ± 0.009	0.102CysAsn: 0.102 ± 0.01	0.274CysPro: 0.274 ± 0.017	0.117CysGln: 0.117 ± 0.009	0.34CysArg: 0.34 ± 0.019	0.306CysSer: 0.306 ± 0.015	0.307CysThr: 0.307 ± 0.016	0.399CysVal: 0.399 ± 0.019	0.064CysTrp: 0.064 ± 0.007	0.099CysTyr: 0.099 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	10.073AspAla: 10.073 ± 0.105	0.249AspCys: 0.249 ± 0.014	4.589AspAsp: 4.589 ± 0.076	4.573AspGlu: 4.573 ± 0.063	1.725AspPhe: 1.725 ± 0.035	6.441AspGly: 6.441 ± 0.086	1.216AspHis: 1.216 ± 0.032	2.581AspIle: 2.581 ± 0.04	0.969AspLys: 0.969 ± 0.034	6.23AspLeu: 6.23 ± 0.067	0.867AspMet: 0.867 ± 0.026	0.995AspAsn: 0.995 ± 0.046	4.343AspPro: 4.343 ± 0.059	1.469AspGln: 1.469 ± 0.034	4.503AspArg: 4.503 ± 0.061	2.529AspSer: 2.529 ± 0.04	2.938AspThr: 2.938 ± 0.049	5.763AspVal: 5.763 ± 0.074	1.024AspTrp: 1.024 ± 0.028	1.288AspTyr: 1.288 ± 0.032	0.0AspXaa: 0.0 ± 0.0
Glu	7.34GluAla: 7.34 ± 0.097	0.226GluCys: 0.226 ± 0.014	2.819GluAsp: 2.819 ± 0.052	2.874GluGlu: 2.874 ± 0.051	1.739GluPhe: 1.739 ± 0.035	4.252GluGly: 4.252 ± 0.061	1.37GluHis: 1.37 ± 0.036	2.616GluIle: 2.616 ± 0.053	1.262GluLys: 1.262 ± 0.04	6.413GluLeu: 6.413 ± 0.075	0.846GluMet: 0.846 ± 0.028	1.132GluAsn: 1.132 ± 0.03	3.258GluPro: 3.258 ± 0.063	1.999GluGln: 1.999 ± 0.038	5.287GluArg: 5.287 ± 0.072	2.803GluSer: 2.803 ± 0.049	2.997GluThr: 2.997 ± 0.046	4.651GluVal: 4.651 ± 0.066	1.009GluTrp: 1.009 ± 0.029	1.259GluTyr: 1.259 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	4.494PheAla: 4.494 ± 0.063	0.158PheCys: 0.158 ± 0.011	2.462PheAsp: 2.462 ± 0.044	1.795PheGlu: 1.795 ± 0.041	1.081PhePhe: 1.081 ± 0.034	3.421PheGly: 3.421 ± 0.056	0.567PheHis: 0.567 ± 0.019	1.146PheIle: 1.146 ± 0.036	0.412PheLys: 0.412 ± 0.021	2.906PheLeu: 2.906 ± 0.046	0.446PheMet: 0.446 ± 0.018	0.656PheAsn: 0.656 ± 0.02	1.436PhePro: 1.436 ± 0.031	0.824PheGln: 0.824 ± 0.025	1.861PheArg: 1.861 ± 0.041	1.697PheSer: 1.697 ± 0.038	2.181PheThr: 2.181 ± 0.047	2.906PheVal: 2.906 ± 0.051	0.525PheTrp: 0.525 ± 0.023	0.656PheTyr: 0.656 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	11.319GlyAla: 11.319 ± 0.114	0.571GlyCys: 0.571 ± 0.024	5.555GlyAsp: 5.555 ± 0.073	5.102GlyGlu: 5.102 ± 0.063	3.357GlyPhe: 3.357 ± 0.055	7.922GlyGly: 7.922 ± 0.101	1.802GlyHis: 1.802 ± 0.039	4.77GlyIle: 4.77 ± 0.062	1.929GlyLys: 1.929 ± 0.048	8.736GlyLeu: 8.736 ± 0.106	1.938GlyMet: 1.938 ± 0.041	1.667GlyAsn: 1.667 ± 0.046	3.996GlyPro: 3.996 ± 0.057	2.342GlyGln: 2.342 ± 0.045	6.397GlyArg: 6.397 ± 0.078	5.304GlySer: 5.304 ± 0.072	5.524GlyThr: 5.524 ± 0.068	8.262GlyVal: 8.262 ± 0.082	1.736GlyTrp: 1.736 ± 0.034	2.272GlyTyr: 2.272 ± 0.04	0.0GlyXaa: 0.0 ± 0.0
His	2.438HisAla: 2.438 ± 0.042	0.093HisCys: 0.093 ± 0.009	1.328HisAsp: 1.328 ± 0.034	1.097HisGlu: 1.097 ± 0.027	0.594HisPhe: 0.594 ± 0.022	1.999HisGly: 1.999 ± 0.043	0.591HisHis: 0.591 ± 0.022	0.73HisIle: 0.73 ± 0.023	0.292HisLys: 0.292 ± 0.016	1.939HisLeu: 1.939 ± 0.04	0.297HisMet: 0.297 ± 0.013	0.351HisAsn: 0.351 ± 0.018	1.479HisPro: 1.479 ± 0.036	0.466HisGln: 0.466 ± 0.018	1.552HisArg: 1.552 ± 0.039	0.9HisSer: 0.9 ± 0.03	0.978HisThr: 0.978 ± 0.031	1.736HisVal: 1.736 ± 0.041	0.301HisTrp: 0.301 ± 0.014	0.432HisTyr: 0.432 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	7.228IleAla: 7.228 ± 0.09	0.202IleCys: 0.202 ± 0.013	3.485IleAsp: 3.485 ± 0.051	2.806IleGlu: 2.806 ± 0.048	1.143IlePhe: 1.143 ± 0.035	4.676IleGly: 4.676 ± 0.071	0.712IleHis: 0.712 ± 0.02	1.682IleIle: 1.682 ± 0.045	0.689IleLys: 0.689 ± 0.025	3.645IleLeu: 3.645 ± 0.055	0.618IleMet: 0.618 ± 0.024	0.835IleAsn: 0.835 ± 0.027	2.573IlePro: 2.573 ± 0.043	0.951IleGln: 0.951 ± 0.029	2.604IleArg: 2.604 ± 0.049	2.212IleSer: 2.212 ± 0.043	2.67IleThr: 2.67 ± 0.045	4.673IleVal: 4.673 ± 0.072	0.491IleTrp: 0.491 ± 0.019	0.732IleTyr: 0.732 ± 0.023	0.0IleXaa: 0.0 ± 0.0
Lys	2.334LysAla: 2.334 ± 0.048	0.053LysCys: 0.053 ± 0.005	1.063LysAsp: 1.063 ± 0.029	0.889LysGlu: 0.889 ± 0.032	0.459LysPhe: 0.459 ± 0.019	1.464LysGly: 1.464 ± 0.04	0.389LysHis: 0.389 ± 0.02	0.774LysIle: 0.774 ± 0.027	0.661LysLys: 0.661 ± 0.026	1.667LysLeu: 1.667 ± 0.037	0.299LysMet: 0.299 ± 0.016	0.434LysAsn: 0.434 ± 0.019	1.185LysPro: 1.185 ± 0.037	0.586LysGln: 0.586 ± 0.026	1.426LysArg: 1.426 ± 0.041	0.984LysSer: 0.984 ± 0.028	1.271LysThr: 1.271 ± 0.037	1.601LysVal: 1.601 ± 0.042	0.237LysTrp: 0.237 ± 0.014	0.437LysTyr: 0.437 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	14.551LeuAla: 14.551 ± 0.13	0.494LeuCys: 0.494 ± 0.019	6.573LeuAsp: 6.573 ± 0.077	5.339LeuGlu: 5.339 ± 0.063	2.931LeuPhe: 2.931 ± 0.059	8.912LeuGly: 8.912 ± 0.094	1.804LeuHis: 1.804 ± 0.039	4.204LeuIle: 4.204 ± 0.067	1.615LeuLys: 1.615 ± 0.039	9.426LeuLeu: 9.426 ± 0.124	1.539LeuMet: 1.539 ± 0.036	1.643LeuAsn: 1.643 ± 0.04	5.411LeuPro: 5.411 ± 0.069	2.451LeuGln: 2.451 ± 0.045	7.45LeuArg: 7.45 ± 0.085	5.531LeuSer: 5.531 ± 0.057	6.114LeuThr: 6.114 ± 0.075	9.258LeuVal: 9.258 ± 0.091	1.409LeuTrp: 1.409 ± 0.04	1.743LeuTyr: 1.743 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	2.047MetAla: 2.047 ± 0.041	0.091MetCys: 0.091 ± 0.009	0.782MetAsp: 0.782 ± 0.025	0.551MetGlu: 0.551 ± 0.022	0.512MetPhe: 0.512 ± 0.021	1.294MetGly: 1.294 ± 0.031	0.326MetHis: 0.326 ± 0.015	0.805MetIle: 0.805 ± 0.026	0.397MetLys: 0.397 ± 0.016	1.823MetLeu: 1.823 ± 0.04	0.299MetMet: 0.299 ± 0.015	0.464MetAsn: 0.464 ± 0.019	1.173MetPro: 1.173 ± 0.03	0.511MetGln: 0.511 ± 0.021	1.332MetArg: 1.332 ± 0.03	1.395MetSer: 1.395 ± 0.033	1.716MetThr: 1.716 ± 0.032	1.229MetVal: 1.229 ± 0.029	0.222MetTrp: 0.222 ± 0.014	0.3MetTyr: 0.3 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.456AsnAla: 2.456 ± 0.049	0.1AsnCys: 0.1 ± 0.009	1.157AsnAsp: 1.157 ± 0.036	0.892AsnGlu: 0.892 ± 0.025	0.624AsnPhe: 0.624 ± 0.023	1.96AsnGly: 1.96 ± 0.051	0.336AsnHis: 0.336 ± 0.015	0.771AsnIle: 0.771 ± 0.026	0.349AsnLys: 0.349 ± 0.016	1.745AsnLeu: 1.745 ± 0.029	0.303AsnMet: 0.303 ± 0.014	0.418AsnAsn: 0.418 ± 0.021	1.565AsnPro: 1.565 ± 0.033	0.515AsnGln: 0.515 ± 0.02	1.186AsnArg: 1.186 ± 0.031	0.954AsnSer: 0.954 ± 0.046	1.084AsnThr: 1.084 ± 0.033	1.59AsnVal: 1.59 ± 0.035	0.296AsnTrp: 0.296 ± 0.016	0.47AsnTyr: 0.47 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	7.922ProAla: 7.922 ± 0.093	0.172ProCys: 0.172 ± 0.013	4.201ProAsp: 4.201 ± 0.063	3.904ProGlu: 3.904 ± 0.059	1.746ProPhe: 1.746 ± 0.032	5.189ProGly: 5.189 ± 0.069	1.159ProHis: 1.159 ± 0.032	2.157ProIle: 2.157 ± 0.043	0.978ProLys: 0.978 ± 0.029	4.865ProLeu: 4.865 ± 0.066	0.919ProMet: 0.919 ± 0.026	0.985ProAsn: 0.985 ± 0.028	2.543ProPro: 2.543 ± 0.059	1.582ProGln: 1.582 ± 0.035	3.568ProArg: 3.568 ± 0.053	3.168ProSer: 3.168 ± 0.05	3.458ProThr: 3.458 ± 0.066	5.036ProVal: 5.036 ± 0.064	0.951ProTrp: 0.951 ± 0.029	1.048ProTyr: 1.048 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	3.401GlnAla: 3.401 ± 0.054	0.101GlnCys: 0.101 ± 0.008	1.249GlnAsp: 1.249 ± 0.03	1.222GlnGlu: 1.222 ± 0.031	0.873GlnPhe: 0.873 ± 0.026	2.015GlnGly: 2.015 ± 0.038	0.613GlnHis: 0.613 ± 0.023	1.249GlnIle: 1.249 ± 0.034	0.57GlnLys: 0.57 ± 0.022	3.017GlnLeu: 3.017 ± 0.047	0.455GlnMet: 0.455 ± 0.018	0.62GlnAsn: 0.62 ± 0.026	1.553GlnPro: 1.553 ± 0.032	1.068GlnGln: 1.068 ± 0.036	2.326GlnArg: 2.326 ± 0.048	1.444GlnSer: 1.444 ± 0.035	1.569GlnThr: 1.569 ± 0.038	2.151GlnVal: 2.151 ± 0.039	0.494GlnTrp: 0.494 ± 0.022	0.574GlnTyr: 0.574 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	9.212ArgAla: 9.212 ± 0.117	0.305ArgCys: 0.305 ± 0.016	4.494ArgAsp: 4.494 ± 0.07	4.427ArgGlu: 4.427 ± 0.072	2.432ArgPhe: 2.432 ± 0.042	5.769ArgGly: 5.769 ± 0.071	1.59ArgHis: 1.59 ± 0.038	3.542ArgIle: 3.542 ± 0.049	1.265ArgLys: 1.265 ± 0.034	7.333ArgLeu: 7.333 ± 0.101	1.627ArgMet: 1.627 ± 0.038	1.263ArgAsn: 1.263 ± 0.03	3.717ArgPro: 3.717 ± 0.055	1.842ArgGln: 1.842 ± 0.033	6.618ArgArg: 6.618 ± 0.112	3.861ArgSer: 3.861 ± 0.056	4.351ArgThr: 4.351 ± 0.067	6.203ArgVal: 6.203 ± 0.069	1.267ArgTrp: 1.267 ± 0.035	1.582ArgTyr: 1.582 ± 0.034	0.0ArgXaa: 0.0 ± 0.0
Ser	7.055SerAla: 7.055 ± 0.083	0.268SerCys: 0.268 ± 0.016	3.16SerAsp: 3.16 ± 0.063	2.586SerGlu: 2.586 ± 0.045	1.878SerPhe: 1.878 ± 0.04	5.685SerGly: 5.685 ± 0.077	1.005SerHis: 1.005 ± 0.028	2.548SerIle: 2.548 ± 0.049	0.969SerLys: 0.969 ± 0.029	5.236SerLeu: 5.236 ± 0.073	1.093SerMet: 1.093 ± 0.029	1.026SerAsn: 1.026 ± 0.027	3.15SerPro: 3.15 ± 0.052	1.374SerGln: 1.374 ± 0.033	3.693SerArg: 3.693 ± 0.048	3.217SerSer: 3.217 ± 0.056	3.51SerThr: 3.51 ± 0.061	4.738SerVal: 4.738 ± 0.062	0.959SerTrp: 0.959 ± 0.029	1.179SerTyr: 1.179 ± 0.032	0.0SerXaa: 0.0 ± 0.0
Thr	8.051ThrAla: 8.051 ± 0.105	0.269ThrCys: 0.269 ± 0.015	3.891ThrAsp: 3.891 ± 0.058	2.824ThrGlu: 2.824 ± 0.042	1.947ThrPhe: 1.947 ± 0.044	5.832ThrGly: 5.832 ± 0.076	1.117ThrHis: 1.117 ± 0.029	2.921ThrIle: 2.921 ± 0.057	1.098ThrLys: 1.098 ± 0.034	5.959ThrLeu: 5.959 ± 0.077	0.911ThrMet: 0.911 ± 0.028	1.147ThrAsn: 1.147 ± 0.029	4.083ThrPro: 4.083 ± 0.07	1.49ThrGln: 1.49 ± 0.034	3.785ThrArg: 3.785 ± 0.062	3.318ThrSer: 3.318 ± 0.054	3.889ThrThr: 3.889 ± 0.064	5.8ThrVal: 5.8 ± 0.084	0.966ThrTrp: 0.966 ± 0.03	1.233ThrTyr: 1.233 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	12.479ValAla: 12.479 ± 0.12	0.501ValCys: 0.501 ± 0.018	5.935ValAsp: 5.935 ± 0.073	4.944ValGlu: 4.944 ± 0.063	2.92ValPhe: 2.92 ± 0.046	7.219ValGly: 7.219 ± 0.075	1.689ValHis: 1.689 ± 0.035	4.289ValIle: 4.289 ± 0.065	1.552ValLys: 1.552 ± 0.037	9.08ValLeu: 9.08 ± 0.102	1.426ValMet: 1.426 ± 0.033	1.823ValAsn: 1.823 ± 0.039	4.995ValPro: 4.995 ± 0.054	2.18ValGln: 2.18 ± 0.04	6.273ValArg: 6.273 ± 0.081	4.836ValSer: 4.836 ± 0.06	5.815ValThr: 5.815 ± 0.067	9.18ValVal: 9.18 ± 0.114	1.305ValTrp: 1.305 ± 0.034	1.627ValTyr: 1.627 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	1.787TrpAla: 1.787 ± 0.038	0.088TrpCys: 0.088 ± 0.007	0.943TrpAsp: 0.943 ± 0.031	0.732TrpGlu: 0.732 ± 0.024	0.653TrpPhe: 0.653 ± 0.022	1.212TrpGly: 1.212 ± 0.031	0.374TrpHis: 0.374 ± 0.018	0.808TrpIle: 0.808 ± 0.027	0.259TrpLys: 0.259 ± 0.016	1.813TrpLeu: 1.813 ± 0.039	0.372TrpMet: 0.372 ± 0.016	0.44TrpAsn: 0.44 ± 0.017	0.757TrpPro: 0.757 ± 0.028	0.566TrpGln: 0.566 ± 0.02	1.32TrpArg: 1.32 ± 0.037	1.015TrpSer: 1.015 ± 0.033	1.038TrpThr: 1.038 ± 0.028	1.265TrpVal: 1.265 ± 0.032	0.41TrpTrp: 0.41 ± 0.018	0.319TrpTyr: 0.319 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.583TyrAla: 2.583 ± 0.051	0.112TyrCys: 0.112 ± 0.009	1.44TyrAsp: 1.44 ± 0.032	1.171TyrGlu: 1.171 ± 0.032	0.757TyrPhe: 0.757 ± 0.024	1.995TyrGly: 1.995 ± 0.039	0.291TyrHis: 0.291 ± 0.017	0.737TyrIle: 0.737 ± 0.024	0.342TyrLys: 0.342 ± 0.019	2.091TyrLeu: 2.091 ± 0.04	0.259TyrMet: 0.259 ± 0.015	0.47TyrAsn: 0.47 ± 0.02	1.008TyrPro: 1.008 ± 0.029	0.527TyrGln: 0.527 ± 0.021	1.706TyrArg: 1.706 ± 0.036	1.06TyrSer: 1.06 ± 0.03	1.209TyrThr: 1.209 ± 0.038	1.694TyrVal: 1.694 ± 0.036	0.36TyrTrp: 0.36 ± 0.018	0.498TyrTyr: 0.498 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4075 proteins (1369450 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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