Alcanivorax sp. IO_7

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Alcanivoracaceae; Alcanivorax; unclassified Alcanivorax

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2056 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M5ZA45|A0A6M5ZA45_9GAMM Uncharacterized protein OS=Alcanivorax sp. IO_7 OX=2735303 GN=HML84_07075 PE=4 SV=1
MM1 pKa = 7.17HH2 pKa = 7.23TLSSPIDD9 pKa = 3.78VDD11 pKa = 5.21DD12 pKa = 5.55PDD14 pKa = 4.12HH15 pKa = 8.11SGMGVFSFAQAGSEE29 pKa = 3.94DD30 pKa = 3.69VWFGEE35 pKa = 4.08WSEE38 pKa = 4.17AGGPNFEE45 pKa = 4.61HH46 pKa = 6.87RR47 pKa = 11.84AVYY50 pKa = 10.16YY51 pKa = 10.55VGEE54 pKa = 4.46KK55 pKa = 10.91GNVDD59 pKa = 2.84ATMPTSGQVDD69 pKa = 3.87YY70 pKa = 10.18TVTGINQHH78 pKa = 5.19TAGGALGGTLTADD91 pKa = 3.92FGGNTVTGSFSSSALSIGVDD111 pKa = 3.41ATISGSSFSGDD122 pKa = 3.28AIAIGGAGTEE132 pKa = 4.28TGVSVGEE139 pKa = 4.11FYY141 pKa = 10.16GTNAAALAGMATFSGNSDD159 pKa = 3.21YY160 pKa = 10.95DD161 pKa = 3.75TAFGGARR168 pKa = 3.41

Molecular weight: 16.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M5ZBP4|A0A6M5ZBP4_9GAMM Uncharacterized protein OS=Alcanivorax sp. IO_7 OX=2735303 GN=HML84_19995 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNIKK11 pKa = 10.12RR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84NHH16 pKa = 5.43GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.16VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.76GRR39 pKa = 11.84KK40 pKa = 8.98RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2056

0

2056

398629

23

964

193.9

21.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.502 ± 0.08

1.161 ± 0.025

6.247 ± 0.059

5.605 ± 0.055

3.181 ± 0.043

8.845 ± 0.069

2.215 ± 0.034

3.81 ± 0.049

2.61 ± 0.05

10.639 ± 0.091

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.304 ± 0.032

2.715 ± 0.043

5.567 ± 0.065

3.509 ± 0.045

8.384 ± 0.086

5.237 ± 0.054

5.309 ± 0.058

7.011 ± 0.049

1.776 ± 0.029

2.373 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski