Amino acid dipepetide frequency for Bacillus virus Wbeta

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.656AlaAla: 4.656 ± 0.935
0.653AlaCys: 0.653 ± 0.316
3.594AlaAsp: 3.594 ± 0.586
5.31AlaGlu: 5.31 ± 0.86
2.941AlaPhe: 2.941 ± 0.406
4.493AlaGly: 4.493 ± 0.848
0.653AlaHis: 0.653 ± 0.214
5.31AlaIle: 5.31 ± 0.551
4.493AlaLys: 4.493 ± 0.697
4.819AlaLeu: 4.819 ± 0.73
1.552AlaMet: 1.552 ± 0.34
3.349AlaAsn: 3.349 ± 0.524
1.389AlaPro: 1.389 ± 0.304
2.042AlaGln: 2.042 ± 0.37
2.859AlaArg: 2.859 ± 0.495
4.329AlaSer: 4.329 ± 0.972
4.003AlaThr: 4.003 ± 0.872
3.839AlaVal: 3.839 ± 0.836
0.98AlaTrp: 0.98 ± 0.287
1.797AlaTyr: 1.797 ± 0.371
0.0AlaXaa: 0.0 ± 0.0
Cys
0.245CysAla: 0.245 ± 0.13
0.082CysCys: 0.082 ± 0.083
0.572CysAsp: 0.572 ± 0.176
0.817CysGlu: 0.817 ± 0.334
0.327CysPhe: 0.327 ± 0.187
0.408CysGly: 0.408 ± 0.186
0.245CysHis: 0.245 ± 0.15
0.49CysIle: 0.49 ± 0.219
0.653CysLys: 0.653 ± 0.198
0.735CysLeu: 0.735 ± 0.4
0.245CysMet: 0.245 ± 0.133
0.327CysAsn: 0.327 ± 0.18
0.653CysPro: 0.653 ± 0.269
0.327CysGln: 0.327 ± 0.132
0.408CysArg: 0.408 ± 0.173
0.653CysSer: 0.653 ± 0.24
0.245CysThr: 0.245 ± 0.127
0.327CysVal: 0.327 ± 0.158
0.0CysTrp: 0.0 ± 0.0
0.817CysTyr: 0.817 ± 0.292
0.0CysXaa: 0.0 ± 0.0
Asp
3.267AspAla: 3.267 ± 0.629
0.327AspCys: 0.327 ± 0.171
3.512AspAsp: 3.512 ± 0.552
4.901AspGlu: 4.901 ± 0.566
2.614AspPhe: 2.614 ± 0.484
3.594AspGly: 3.594 ± 0.468
0.572AspHis: 0.572 ± 0.182
4.003AspIle: 4.003 ± 0.548
5.963AspLys: 5.963 ± 0.599
4.983AspLeu: 4.983 ± 0.644
2.042AspMet: 2.042 ± 0.363
2.287AspAsn: 2.287 ± 0.414
1.225AspPro: 1.225 ± 0.36
2.042AspGln: 2.042 ± 0.429
2.859AspArg: 2.859 ± 0.474
2.696AspSer: 2.696 ± 0.361
2.941AspThr: 2.941 ± 0.515
4.084AspVal: 4.084 ± 0.488
0.653AspTrp: 0.653 ± 0.227
2.042AspTyr: 2.042 ± 0.561
0.0AspXaa: 0.0 ± 0.0
Glu
5.146GluAla: 5.146 ± 0.654
0.98GluCys: 0.98 ± 0.371
4.329GluAsp: 4.329 ± 0.554
7.924GluGlu: 7.924 ± 1.094
2.777GluPhe: 2.777 ± 0.44
4.084GluGly: 4.084 ± 0.587
1.634GluHis: 1.634 ± 0.278
7.597GluIle: 7.597 ± 0.714
8.25GluLys: 8.25 ± 0.893
8.659GluLeu: 8.659 ± 0.866
3.267GluMet: 3.267 ± 0.455
4.248GluAsn: 4.248 ± 0.591
1.062GluPro: 1.062 ± 0.404
4.329GluGln: 4.329 ± 0.815
3.758GluArg: 3.758 ± 0.631
4.166GluSer: 4.166 ± 0.784
4.248GluThr: 4.248 ± 0.603
4.819GluVal: 4.819 ± 0.595
0.653GluTrp: 0.653 ± 0.225
2.451GluTyr: 2.451 ± 0.423
0.0GluXaa: 0.0 ± 0.0
Phe
2.532PheAla: 2.532 ± 0.447
0.408PheCys: 0.408 ± 0.175
2.614PheAsp: 2.614 ± 0.573
2.451PheGlu: 2.451 ± 0.385
1.144PhePhe: 1.144 ± 0.336
2.369PheGly: 2.369 ± 0.428
0.653PheHis: 0.653 ± 0.195
2.369PheIle: 2.369 ± 0.525
3.758PheLys: 3.758 ± 0.433
3.758PheLeu: 3.758 ± 0.585
1.307PheMet: 1.307 ± 0.307
2.369PheAsn: 2.369 ± 0.43
1.062PhePro: 1.062 ± 0.29
1.225PheGln: 1.225 ± 0.351
1.96PheArg: 1.96 ± 0.362
2.124PheSer: 2.124 ± 0.41
2.206PheThr: 2.206 ± 0.487
1.96PheVal: 1.96 ± 0.356
0.408PheTrp: 0.408 ± 0.208
1.552PheTyr: 1.552 ± 0.31
0.0PheXaa: 0.0 ± 0.0
Gly
3.758GlyAla: 3.758 ± 0.573
0.327GlyCys: 0.327 ± 0.196
3.186GlyAsp: 3.186 ± 0.508
5.065GlyGlu: 5.065 ± 0.711
3.676GlyPhe: 3.676 ± 0.504
3.758GlyGly: 3.758 ± 0.685
0.98GlyHis: 0.98 ± 0.233
6.372GlyIle: 6.372 ± 1.916
5.8GlyLys: 5.8 ± 0.556
5.881GlyLeu: 5.881 ± 0.975
2.206GlyMet: 2.206 ± 0.61
2.777GlyAsn: 2.777 ± 0.526
3.921GlyPro: 3.921 ± 3.067
1.715GlyGln: 1.715 ± 0.318
1.797GlyArg: 1.797 ± 0.322
3.267GlySer: 3.267 ± 0.529
2.532GlyThr: 2.532 ± 0.471
4.574GlyVal: 4.574 ± 0.822
0.735GlyTrp: 0.735 ± 0.292
2.206GlyTyr: 2.206 ± 0.409
0.0GlyXaa: 0.0 ± 0.0
His
0.98HisAla: 0.98 ± 0.24
0.572HisCys: 0.572 ± 0.258
0.49HisAsp: 0.49 ± 0.19
1.47HisGlu: 1.47 ± 0.358
0.653HisPhe: 0.653 ± 0.233
0.49HisGly: 0.49 ± 0.164
0.572HisHis: 0.572 ± 0.258
1.144HisIle: 1.144 ± 0.333
0.98HisLys: 0.98 ± 0.227
1.225HisLeu: 1.225 ± 0.232
0.572HisMet: 0.572 ± 0.223
0.98HisAsn: 0.98 ± 0.318
0.572HisPro: 0.572 ± 0.338
0.572HisGln: 0.572 ± 0.197
0.98HisArg: 0.98 ± 0.236
0.49HisSer: 0.49 ± 0.243
0.98HisThr: 0.98 ± 0.317
0.572HisVal: 0.572 ± 0.2
0.245HisTrp: 0.245 ± 0.124
1.225HisTyr: 1.225 ± 0.261
0.0HisXaa: 0.0 ± 0.0
Ile
5.31IleAla: 5.31 ± 0.592
0.653IleCys: 0.653 ± 0.217
4.901IleAsp: 4.901 ± 0.714
6.453IleGlu: 6.453 ± 0.765
1.552IlePhe: 1.552 ± 0.394
4.003IleGly: 4.003 ± 0.606
0.899IleHis: 0.899 ± 0.243
3.022IleIle: 3.022 ± 0.472
6.535IleLys: 6.535 ± 0.655
5.555IleLeu: 5.555 ± 0.795
1.225IleMet: 1.225 ± 0.371
4.329IleAsn: 4.329 ± 0.542
3.431IlePro: 3.431 ± 0.996
4.248IleGln: 4.248 ± 1.495
4.248IleArg: 4.248 ± 0.466
4.901IleSer: 4.901 ± 0.604
3.267IleThr: 3.267 ± 0.489
3.839IleVal: 3.839 ± 0.55
0.735IleTrp: 0.735 ± 0.269
2.042IleTyr: 2.042 ± 0.523
0.0IleXaa: 0.0 ± 0.0
Lys
5.718LysAla: 5.718 ± 0.908
0.653LysCys: 0.653 ± 0.261
3.839LysAsp: 3.839 ± 0.598
8.985LysGlu: 8.985 ± 0.99
2.941LysPhe: 2.941 ± 0.423
5.473LysGly: 5.473 ± 0.723
1.634LysHis: 1.634 ± 0.412
7.025LysIle: 7.025 ± 0.818
9.557LysLys: 9.557 ± 0.896
8.659LysLeu: 8.659 ± 0.967
2.859LysMet: 2.859 ± 0.45
5.391LysAsn: 5.391 ± 0.724
2.369LysPro: 2.369 ± 0.473
3.104LysGln: 3.104 ± 0.561
4.084LysArg: 4.084 ± 0.604
4.983LysSer: 4.983 ± 0.549
4.819LysThr: 4.819 ± 0.863
6.045LysVal: 6.045 ± 0.681
0.98LysTrp: 0.98 ± 0.278
3.104LysTyr: 3.104 ± 0.626
0.0LysXaa: 0.0 ± 0.0
Leu
5.065LeuAla: 5.065 ± 0.742
0.653LeuCys: 0.653 ± 0.315
4.983LeuAsp: 4.983 ± 0.621
7.107LeuGlu: 7.107 ± 0.757
3.676LeuPhe: 3.676 ± 0.68
4.738LeuGly: 4.738 ± 0.627
1.144LeuHis: 1.144 ± 0.307
4.166LeuIle: 4.166 ± 0.586
8.822LeuLys: 8.822 ± 0.804
6.045LeuLeu: 6.045 ± 0.987
1.715LeuMet: 1.715 ± 0.304
5.228LeuAsn: 5.228 ± 0.704
2.124LeuPro: 2.124 ± 0.448
4.411LeuGln: 4.411 ± 0.724
3.431LeuArg: 3.431 ± 0.56
5.228LeuSer: 5.228 ± 0.578
6.29LeuThr: 6.29 ± 0.516
4.656LeuVal: 4.656 ± 0.598
0.735LeuTrp: 0.735 ± 0.298
3.186LeuTyr: 3.186 ± 0.614
0.0LeuXaa: 0.0 ± 0.0
Met
1.307MetAla: 1.307 ± 0.381
0.082MetCys: 0.082 ± 0.086
1.96MetAsp: 1.96 ± 0.402
1.96MetGlu: 1.96 ± 0.327
0.572MetPhe: 0.572 ± 0.356
1.797MetGly: 1.797 ± 0.384
0.408MetHis: 0.408 ± 0.219
1.96MetIle: 1.96 ± 0.273
3.349MetLys: 3.349 ± 0.559
1.389MetLeu: 1.389 ± 0.288
0.572MetMet: 0.572 ± 0.212
1.389MetAsn: 1.389 ± 0.354
0.817MetPro: 0.817 ± 0.219
1.225MetGln: 1.225 ± 0.347
1.634MetArg: 1.634 ± 0.312
2.206MetSer: 2.206 ± 0.367
1.96MetThr: 1.96 ± 0.411
1.144MetVal: 1.144 ± 0.282
0.735MetTrp: 0.735 ± 0.251
0.572MetTyr: 0.572 ± 0.244
0.0MetXaa: 0.0 ± 0.0
Asn
3.921AsnAla: 3.921 ± 0.601
0.0AsnCys: 0.0 ± 0.0
2.451AsnAsp: 2.451 ± 0.419
4.738AsnGlu: 4.738 ± 0.686
1.715AsnPhe: 1.715 ± 0.405
3.512AsnGly: 3.512 ± 0.518
1.307AsnHis: 1.307 ± 0.447
3.022AsnIle: 3.022 ± 0.38
4.656AsnLys: 4.656 ± 0.641
3.839AsnLeu: 3.839 ± 0.494
1.634AsnMet: 1.634 ± 0.409
2.451AsnAsn: 2.451 ± 0.488
2.287AsnPro: 2.287 ± 0.43
2.369AsnGln: 2.369 ± 0.466
4.166AsnArg: 4.166 ± 0.663
2.696AsnSer: 2.696 ± 0.55
2.532AsnThr: 2.532 ± 0.47
3.839AsnVal: 3.839 ± 0.647
0.735AsnTrp: 0.735 ± 0.266
1.47AsnTyr: 1.47 ± 0.43
0.0AsnXaa: 0.0 ± 0.0
Pro
1.96ProAla: 1.96 ± 0.38
0.327ProCys: 0.327 ± 0.203
0.899ProAsp: 0.899 ± 0.308
2.451ProGlu: 2.451 ± 0.489
0.98ProPhe: 0.98 ± 0.268
2.614ProGly: 2.614 ± 1.335
0.735ProHis: 0.735 ± 0.251
2.287ProIle: 2.287 ± 0.543
1.389ProLys: 1.389 ± 0.384
1.96ProLeu: 1.96 ± 0.472
0.653ProMet: 0.653 ± 0.208
1.47ProAsn: 1.47 ± 0.318
0.899ProPro: 0.899 ± 0.283
1.389ProGln: 1.389 ± 0.431
0.98ProArg: 0.98 ± 0.282
2.369ProSer: 2.369 ± 0.402
3.512ProThr: 3.512 ± 1.841
1.797ProVal: 1.797 ± 0.385
0.163ProTrp: 0.163 ± 0.175
1.389ProTyr: 1.389 ± 0.42
0.0ProXaa: 0.0 ± 0.0
Gln
3.104GlnAla: 3.104 ± 0.867
0.245GlnCys: 0.245 ± 0.152
1.879GlnAsp: 1.879 ± 0.355
3.349GlnGlu: 3.349 ± 0.571
1.715GlnPhe: 1.715 ± 0.431
4.983GlnGly: 4.983 ± 2.613
0.817GlnHis: 0.817 ± 0.243
2.042GlnIle: 2.042 ± 0.314
4.329GlnLys: 4.329 ± 0.692
2.532GlnLeu: 2.532 ± 0.514
1.389GlnMet: 1.389 ± 0.328
2.206GlnAsn: 2.206 ± 0.448
1.715GlnPro: 1.715 ± 0.299
2.451GlnGln: 2.451 ± 0.75
1.715GlnArg: 1.715 ± 0.387
2.777GlnSer: 2.777 ± 0.552
1.552GlnThr: 1.552 ± 0.339
2.532GlnVal: 2.532 ± 0.468
0.082GlnTrp: 0.082 ± 0.081
1.552GlnTyr: 1.552 ± 0.401
0.0GlnXaa: 0.0 ± 0.0
Arg
2.532ArgAla: 2.532 ± 0.489
0.327ArgCys: 0.327 ± 0.148
3.104ArgAsp: 3.104 ± 0.456
4.574ArgGlu: 4.574 ± 0.536
1.96ArgPhe: 1.96 ± 0.409
2.777ArgGly: 2.777 ± 0.462
0.572ArgHis: 0.572 ± 0.231
3.758ArgIle: 3.758 ± 0.631
3.921ArgLys: 3.921 ± 0.832
4.656ArgLeu: 4.656 ± 0.62
1.062ArgMet: 1.062 ± 0.272
2.532ArgAsn: 2.532 ± 0.574
1.062ArgPro: 1.062 ± 0.293
2.042ArgGln: 2.042 ± 0.587
1.797ArgArg: 1.797 ± 0.378
2.614ArgSer: 2.614 ± 0.663
1.96ArgThr: 1.96 ± 0.384
2.369ArgVal: 2.369 ± 0.426
0.653ArgTrp: 0.653 ± 0.221
2.206ArgTyr: 2.206 ± 0.458
0.0ArgXaa: 0.0 ± 0.0
Ser
3.431SerAla: 3.431 ± 0.774
0.408SerCys: 0.408 ± 0.174
3.839SerAsp: 3.839 ± 0.424
3.431SerGlu: 3.431 ± 0.547
2.206SerPhe: 2.206 ± 0.492
3.676SerGly: 3.676 ± 0.641
0.98SerHis: 0.98 ± 0.285
3.839SerIle: 3.839 ± 0.614
6.617SerLys: 6.617 ± 0.901
5.555SerLeu: 5.555 ± 0.579
1.634SerMet: 1.634 ± 0.414
3.186SerAsn: 3.186 ± 0.568
1.144SerPro: 1.144 ± 0.335
2.287SerGln: 2.287 ± 0.393
2.287SerArg: 2.287 ± 0.408
3.267SerSer: 3.267 ± 0.606
3.758SerThr: 3.758 ± 0.527
3.921SerVal: 3.921 ± 0.48
0.572SerTrp: 0.572 ± 0.221
1.715SerTyr: 1.715 ± 0.385
0.0SerXaa: 0.0 ± 0.0
Thr
3.839ThrAla: 3.839 ± 0.713
0.163ThrCys: 0.163 ± 0.11
2.777ThrAsp: 2.777 ± 0.435
4.819ThrGlu: 4.819 ± 0.933
2.206ThrPhe: 2.206 ± 0.443
6.453ThrGly: 6.453 ± 2.381
0.572ThrHis: 0.572 ± 0.248
4.738ThrIle: 4.738 ± 0.564
4.574ThrLys: 4.574 ± 0.596
4.656ThrLeu: 4.656 ± 0.693
1.225ThrMet: 1.225 ± 0.288
2.451ThrAsn: 2.451 ± 0.494
2.042ThrPro: 2.042 ± 0.506
2.532ThrGln: 2.532 ± 0.4
2.042ThrArg: 2.042 ± 0.336
2.532ThrSer: 2.532 ± 0.369
4.003ThrThr: 4.003 ± 0.609
3.758ThrVal: 3.758 ± 0.42
0.082ThrTrp: 0.082 ± 0.089
1.797ThrTyr: 1.797 ± 0.329
0.0ThrXaa: 0.0 ± 0.0
Val
3.267ValAla: 3.267 ± 0.657
0.653ValCys: 0.653 ± 0.302
4.819ValAsp: 4.819 ± 0.601
5.065ValGlu: 5.065 ± 0.648
2.287ValPhe: 2.287 ± 0.456
2.941ValGly: 2.941 ± 0.487
0.653ValHis: 0.653 ± 0.267
4.738ValIle: 4.738 ± 0.575
4.411ValLys: 4.411 ± 0.537
4.656ValLeu: 4.656 ± 0.745
0.98ValMet: 0.98 ± 0.273
3.349ValAsn: 3.349 ± 0.53
1.634ValPro: 1.634 ± 0.364
3.022ValGln: 3.022 ± 0.607
3.186ValArg: 3.186 ± 0.432
4.166ValSer: 4.166 ± 0.588
3.758ValThr: 3.758 ± 0.53
3.676ValVal: 3.676 ± 0.512
0.653ValTrp: 0.653 ± 0.241
2.859ValTyr: 2.859 ± 0.562
0.0ValXaa: 0.0 ± 0.0
Trp
0.49TrpAla: 0.49 ± 0.294
0.163TrpCys: 0.163 ± 0.11
0.817TrpAsp: 0.817 ± 0.211
0.817TrpGlu: 0.817 ± 0.243
0.49TrpPhe: 0.49 ± 0.223
0.49TrpGly: 0.49 ± 0.21
0.245TrpHis: 0.245 ± 0.155
0.98TrpIle: 0.98 ± 0.411
0.572TrpLys: 0.572 ± 0.189
0.817TrpLeu: 0.817 ± 0.262
0.0TrpMet: 0.0 ± 0.0
0.98TrpAsn: 0.98 ± 0.346
0.082TrpPro: 0.082 ± 0.082
0.245TrpGln: 0.245 ± 0.159
0.49TrpArg: 0.49 ± 0.2
1.062TrpSer: 1.062 ± 0.288
0.572TrpThr: 0.572 ± 0.214
0.572TrpVal: 0.572 ± 0.228
0.163TrpTrp: 0.163 ± 0.13
0.408TrpTyr: 0.408 ± 0.164
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.532TyrAla: 2.532 ± 0.497
0.899TyrCys: 0.899 ± 0.328
2.042TyrAsp: 2.042 ± 0.439
2.696TyrGlu: 2.696 ± 0.448
1.797TyrPhe: 1.797 ± 0.398
1.879TyrGly: 1.879 ± 0.342
0.49TyrHis: 0.49 ± 0.149
2.369TyrIle: 2.369 ± 0.448
3.594TyrLys: 3.594 ± 0.762
3.022TyrLeu: 3.022 ± 0.595
0.817TyrMet: 0.817 ± 0.186
2.042TyrAsn: 2.042 ± 0.429
0.735TyrPro: 0.735 ± 0.259
1.389TyrGln: 1.389 ± 0.352
1.797TyrArg: 1.797 ± 0.395
1.225TyrSer: 1.225 ± 0.203
2.124TyrThr: 2.124 ± 0.355
2.451TyrVal: 2.451 ± 0.314
0.49TyrTrp: 0.49 ± 0.171
1.389TyrTyr: 1.389 ± 0.574
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (12243 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski