Amino acid dipepetide frequency for Anabaena sp. 90

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.524AlaAla: 6.524 ± 0.091	0.74AlaCys: 0.74 ± 0.023	3.858AlaAsp: 3.858 ± 0.058	4.914AlaGlu: 4.914 ± 0.061	2.707AlaPhe: 2.707 ± 0.051	4.948AlaGly: 4.948 ± 0.076	1.085AlaHis: 1.085 ± 0.03	6.503AlaIle: 6.503 ± 0.09	4.49AlaLys: 4.49 ± 0.066	7.383AlaLeu: 7.383 ± 0.085	1.481AlaMet: 1.481 ± 0.039	3.601AlaAsn: 3.601 ± 0.066	2.438AlaPro: 2.438 ± 0.05	3.376AlaGln: 3.376 ± 0.052	2.922AlaArg: 2.922 ± 0.05	3.976AlaSer: 3.976 ± 0.061	4.214AlaThr: 4.214 ± 0.064	5.049AlaVal: 5.049 ± 0.064	0.935AlaTrp: 0.935 ± 0.03	2.341AlaTyr: 2.341 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.593CysAla: 0.593 ± 0.022	0.161CysCys: 0.161 ± 0.011	0.555CysAsp: 0.555 ± 0.02	0.538CysGlu: 0.538 ± 0.022	0.414CysPhe: 0.414 ± 0.018	0.812CysGly: 0.812 ± 0.027	0.279CysHis: 0.279 ± 0.015	0.633CysIle: 0.633 ± 0.023	0.384CysLys: 0.384 ± 0.017	1.175CysLeu: 1.175 ± 0.031	0.158CysMet: 0.158 ± 0.013	0.394CysAsn: 0.394 ± 0.017	0.522CysPro: 0.522 ± 0.022	0.653CysGln: 0.653 ± 0.023	0.514CysArg: 0.514 ± 0.023	0.598CysSer: 0.598 ± 0.017	0.489CysThr: 0.489 ± 0.019	0.58CysVal: 0.58 ± 0.022	0.143CysTrp: 0.143 ± 0.01	0.357CysTyr: 0.357 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.3AspAla: 3.3 ± 0.051	0.542AspCys: 0.542 ± 0.023	2.299AspAsp: 2.299 ± 0.056	2.928AspGlu: 2.928 ± 0.06	2.638AspPhe: 2.638 ± 0.053	3.123AspGly: 3.123 ± 0.063	0.872AspHis: 0.872 ± 0.024	4.025AspIle: 4.025 ± 0.065	2.856AspLys: 2.856 ± 0.053	5.828AspLeu: 5.828 ± 0.071	0.78AspMet: 0.78 ± 0.023	2.486AspAsn: 2.486 ± 0.061	2.111AspPro: 2.111 ± 0.042	1.906AspGln: 1.906 ± 0.041	2.473AspArg: 2.473 ± 0.044	2.901AspSer: 2.901 ± 0.05	2.505AspThr: 2.505 ± 0.056	2.95AspVal: 2.95 ± 0.054	0.909AspTrp: 0.909 ± 0.025	1.948AspTyr: 1.948 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	4.686GluAla: 4.686 ± 0.076	0.495GluCys: 0.495 ± 0.017	2.769GluAsp: 2.769 ± 0.058	4.194GluGlu: 4.194 ± 0.069	2.607GluPhe: 2.607 ± 0.048	3.024GluGly: 3.024 ± 0.058	0.957GluHis: 0.957 ± 0.029	5.699GluIle: 5.699 ± 0.076	4.286GluLys: 4.286 ± 0.067	7.211GluLeu: 7.211 ± 0.087	1.395GluMet: 1.395 ± 0.033	3.563GluAsn: 3.563 ± 0.057	2.159GluPro: 2.159 ± 0.04	3.212GluGln: 3.212 ± 0.059	3.062GluArg: 3.062 ± 0.053	3.545GluSer: 3.545 ± 0.058	3.723GluThr: 3.723 ± 0.055	4.152GluVal: 4.152 ± 0.063	0.79GluTrp: 0.79 ± 0.025	2.099GluTyr: 2.099 ± 0.04	0.0GluXaa: 0.0 ± 0.0
Phe	2.974PheAla: 2.974 ± 0.05	0.546PheCys: 0.546 ± 0.02	2.325PheAsp: 2.325 ± 0.043	2.077PheGlu: 2.077 ± 0.045	1.68PhePhe: 1.68 ± 0.036	2.821PheGly: 2.821 ± 0.048	0.822PheHis: 0.822 ± 0.029	2.778PheIle: 2.778 ± 0.043	1.722PheLys: 1.722 ± 0.034	4.302PheLeu: 4.302 ± 0.065	0.746PheMet: 0.746 ± 0.025	2.052PheAsn: 2.052 ± 0.041	1.946PhePro: 1.946 ± 0.037	1.904PheGln: 1.904 ± 0.043	1.772PheArg: 1.772 ± 0.039	2.959PheSer: 2.959 ± 0.049	2.488PheThr: 2.488 ± 0.056	2.356PheVal: 2.356 ± 0.045	0.665PheTrp: 0.665 ± 0.023	1.424PheTyr: 1.424 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	4.221GlyAla: 4.221 ± 0.076	0.77GlyCys: 0.77 ± 0.028	3.267GlyAsp: 3.267 ± 0.064	4.187GlyGlu: 4.187 ± 0.065	2.908GlyPhe: 2.908 ± 0.045	4.563GlyGly: 4.563 ± 0.097	1.16GlyHis: 1.16 ± 0.036	5.286GlyIle: 5.286 ± 0.064	4.558GlyLys: 4.558 ± 0.061	6.619GlyLeu: 6.619 ± 0.077	1.426GlyMet: 1.426 ± 0.036	3.495GlyAsn: 3.495 ± 0.103	1.104GlyPro: 1.104 ± 0.031	2.686GlyGln: 2.686 ± 0.04	2.869GlyArg: 2.869 ± 0.048	3.735GlySer: 3.735 ± 0.06	3.735GlyThr: 3.735 ± 0.075	4.518GlyVal: 4.518 ± 0.055	1.05GlyTrp: 1.05 ± 0.033	2.301GlyTyr: 2.301 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	0.936HisAla: 0.936 ± 0.03	0.277HisCys: 0.277 ± 0.016	0.794HisAsp: 0.794 ± 0.026	0.939HisGlu: 0.939 ± 0.028	0.796HisPhe: 0.796 ± 0.026	1.146HisGly: 1.146 ± 0.028	0.665HisHis: 0.665 ± 0.025	1.295HisIle: 1.295 ± 0.031	0.914HisLys: 0.914 ± 0.028	2.232HisLeu: 2.232 ± 0.032	0.173HisMet: 0.173 ± 0.01	0.871HisAsn: 0.871 ± 0.026	1.262HisPro: 1.262 ± 0.033	1.215HisGln: 1.215 ± 0.032	1.011HisArg: 1.011 ± 0.027	1.205HisSer: 1.205 ± 0.035	0.921HisThr: 0.921 ± 0.028	0.758HisVal: 0.758 ± 0.024	0.327HisTrp: 0.327 ± 0.018	0.69HisTyr: 0.69 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	6.776IleAla: 6.776 ± 0.073	0.864IleCys: 0.864 ± 0.025	4.254IleAsp: 4.254 ± 0.061	4.63IleGlu: 4.63 ± 0.056	2.99IlePhe: 2.99 ± 0.056	4.609IleGly: 4.609 ± 0.06	1.407IleHis: 1.407 ± 0.035	5.064IleIle: 5.064 ± 0.067	4.036IleLys: 4.036 ± 0.063	7.436IleLeu: 7.436 ± 0.081	1.095IleMet: 1.095 ± 0.031	4.035IleAsn: 4.035 ± 0.069	3.857IlePro: 3.857 ± 0.055	3.373IleGln: 3.373 ± 0.056	3.14IleArg: 3.14 ± 0.056	5.241IleSer: 5.241 ± 0.074	4.507IleThr: 4.507 ± 0.059	4.373IleVal: 4.373 ± 0.059	0.989IleTrp: 0.989 ± 0.031	2.302IleTyr: 2.302 ± 0.041	0.0IleXaa: 0.0 ± 0.0
Lys	4.052LysAla: 4.052 ± 0.063	0.417LysCys: 0.417 ± 0.02	2.575LysAsp: 2.575 ± 0.054	3.261LysGlu: 3.261 ± 0.064	2.201LysPhe: 2.201 ± 0.042	2.905LysGly: 2.905 ± 0.05	0.908LysHis: 0.908 ± 0.029	4.573LysIle: 4.573 ± 0.069	2.958LysLys: 2.958 ± 0.056	6.223LysLeu: 6.223 ± 0.075	1.139LysMet: 1.139 ± 0.031	2.933LysAsn: 2.933 ± 0.06	2.595LysPro: 2.595 ± 0.045	2.967LysGln: 2.967 ± 0.048	2.468LysArg: 2.468 ± 0.045	3.582LysSer: 3.582 ± 0.056	3.381LysThr: 3.381 ± 0.059	3.314LysVal: 3.314 ± 0.053	0.613LysTrp: 0.613 ± 0.021	1.882LysTyr: 1.882 ± 0.044	0.0LysXaa: 0.0 ± 0.0
Leu	8.778LeuAla: 8.778 ± 0.093	0.984LeuCys: 0.984 ± 0.028	5.353LeuAsp: 5.353 ± 0.07	7.444LeuGlu: 7.444 ± 0.081	3.861LeuPhe: 3.861 ± 0.069	7.41LeuGly: 7.41 ± 0.09	1.803LeuHis: 1.803 ± 0.037	7.451LeuIle: 7.451 ± 0.088	5.985LeuLys: 5.985 ± 0.077	10.929LeuLeu: 10.929 ± 0.123	2.046LeuMet: 2.046 ± 0.046	5.029LeuAsn: 5.029 ± 0.065	5.659LeuPro: 5.659 ± 0.082	5.714LeuGln: 5.714 ± 0.082	4.955LeuArg: 4.955 ± 0.069	7.454LeuSer: 7.454 ± 0.088	6.611LeuThr: 6.611 ± 0.091	6.804LeuVal: 6.804 ± 0.075	1.41LeuTrp: 1.41 ± 0.042	2.675LeuTyr: 2.675 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	1.575MetAla: 1.575 ± 0.036	0.116MetCys: 0.116 ± 0.008	0.7MetAsp: 0.7 ± 0.024	1.019MetGlu: 1.019 ± 0.03	0.572MetPhe: 0.572 ± 0.024	1.366MetGly: 1.366 ± 0.034	0.242MetHis: 0.242 ± 0.013	1.34MetIle: 1.34 ± 0.036	1.038MetLys: 1.038 ± 0.028	1.942MetLeu: 1.942 ± 0.044	0.469MetMet: 0.469 ± 0.019	0.998MetAsn: 0.998 ± 0.029	0.814MetPro: 0.814 ± 0.022	0.905MetGln: 0.905 ± 0.027	0.902MetArg: 0.902 ± 0.024	1.304MetSer: 1.304 ± 0.029	1.333MetThr: 1.333 ± 0.034	1.236MetVal: 1.236 ± 0.031	0.154MetTrp: 0.154 ± 0.01	0.392MetTyr: 0.392 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.977AsnAla: 2.977 ± 0.054	0.578AsnCys: 0.578 ± 0.025	2.01AsnAsp: 2.01 ± 0.052	2.124AsnGlu: 2.124 ± 0.052	2.284AsnPhe: 2.284 ± 0.05	2.921AsnGly: 2.921 ± 0.071	1.098AsnHis: 1.098 ± 0.028	3.701AsnIle: 3.701 ± 0.057	2.384AsnLys: 2.384 ± 0.05	6.103AsnLeu: 6.103 ± 0.088	0.784AsnMet: 0.784 ± 0.024	2.809AsnAsn: 2.809 ± 0.071	3.12AsnPro: 3.12 ± 0.06	3.17AsnGln: 3.17 ± 0.056	2.453AsnArg: 2.453 ± 0.045	3.677AsnSer: 3.677 ± 0.065	2.714AsnThr: 2.714 ± 0.054	2.326AsnVal: 2.326 ± 0.041	0.923AsnTrp: 0.923 ± 0.027	1.959AsnTyr: 1.959 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	2.961ProAla: 2.961 ± 0.056	0.334ProCys: 0.334 ± 0.015	2.859ProAsp: 2.859 ± 0.053	3.776ProGlu: 3.776 ± 0.06	1.645ProPhe: 1.645 ± 0.038	2.914ProGly: 2.914 ± 0.055	0.944ProHis: 0.944 ± 0.026	3.199ProIle: 3.199 ± 0.053	2.284ProLys: 2.284 ± 0.043	4.507ProLeu: 4.507 ± 0.07	0.73ProMet: 0.73 ± 0.024	2.315ProAsn: 2.315 ± 0.05	2.035ProPro: 2.035 ± 0.053	2.654ProGln: 2.654 ± 0.048	1.536ProArg: 1.536 ± 0.036	2.732ProSer: 2.732 ± 0.048	2.839ProThr: 2.839 ± 0.066	3.154ProVal: 3.154 ± 0.051	0.556ProTrp: 0.556 ± 0.02	1.328ProTyr: 1.328 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	4.055GlnAla: 4.055 ± 0.07	0.338GlnCys: 0.338 ± 0.017	2.216GlnAsp: 2.216 ± 0.043	4.024GlnGlu: 4.024 ± 0.062	1.78GlnPhe: 1.78 ± 0.037	3.117GlnGly: 3.117 ± 0.054	0.858GlnHis: 0.858 ± 0.029	4.147GlnIle: 4.147 ± 0.059	3.232GlnLys: 3.232 ± 0.056	5.818GlnLeu: 5.818 ± 0.09	1.018GlnMet: 1.018 ± 0.033	2.456GlnAsn: 2.456 ± 0.046	2.472GlnPro: 2.472 ± 0.044	3.577GlnGln: 3.577 ± 0.076	2.543GlnArg: 2.543 ± 0.05	2.941GlnSer: 2.941 ± 0.047	2.877GlnThr: 2.877 ± 0.05	3.61GlnVal: 3.61 ± 0.046	0.641GlnTrp: 0.641 ± 0.023	1.332GlnTyr: 1.332 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	2.628ArgAla: 2.628 ± 0.052	0.445ArgCys: 0.445 ± 0.018	2.246ArgAsp: 2.246 ± 0.047	3.186ArgGlu: 3.186 ± 0.057	2.01ArgPhe: 2.01 ± 0.04	2.69ArgGly: 2.69 ± 0.058	0.907ArgHis: 0.907 ± 0.031	3.247ArgIle: 3.247 ± 0.046	2.411ArgLys: 2.411 ± 0.051	5.302ArgLeu: 5.302 ± 0.064	0.898ArgMet: 0.898 ± 0.026	2.117ArgAsn: 2.117 ± 0.042	1.804ArgPro: 1.804 ± 0.042	2.813ArgGln: 2.813 ± 0.052	2.54ArgArg: 2.54 ± 0.056	2.782ArgSer: 2.782 ± 0.045	2.324ArgThr: 2.324 ± 0.052	2.886ArgVal: 2.886 ± 0.049	0.686ArgTrp: 0.686 ± 0.022	1.772ArgTyr: 1.772 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	4.02SerAla: 4.02 ± 0.058	0.686SerCys: 0.686 ± 0.025	3.074SerAsp: 3.074 ± 0.053	3.886SerGlu: 3.886 ± 0.056	2.525SerPhe: 2.525 ± 0.043	4.608SerGly: 4.608 ± 0.08	1.342SerHis: 1.342 ± 0.034	4.121SerIle: 4.121 ± 0.061	2.923SerLys: 2.923 ± 0.056	7.499SerLeu: 7.499 ± 0.081	1.185SerMet: 1.185 ± 0.03	2.849SerAsn: 2.849 ± 0.054	3.393SerPro: 3.393 ± 0.056	3.875SerGln: 3.875 ± 0.06	2.904SerArg: 2.904 ± 0.041	4.472SerSer: 4.472 ± 0.074	3.319SerThr: 3.319 ± 0.056	3.91SerVal: 3.91 ± 0.052	0.991SerTrp: 0.991 ± 0.025	1.914SerTyr: 1.914 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	4.788ThrAla: 4.788 ± 0.067	0.456ThrCys: 0.456 ± 0.018	2.859ThrAsp: 2.859 ± 0.053	3.544ThrGlu: 3.544 ± 0.058	2.182ThrPhe: 2.182 ± 0.041	4.316ThrGly: 4.316 ± 0.067	1.003ThrHis: 1.003 ± 0.025	4.129ThrIle: 4.129 ± 0.06	2.692ThrLys: 2.692 ± 0.051	6.028ThrLeu: 6.028 ± 0.08	0.82ThrMet: 0.82 ± 0.025	2.767ThrAsn: 2.767 ± 0.059	3.355ThrPro: 3.355 ± 0.055	2.858ThrGln: 2.858 ± 0.046	2.169ThrArg: 2.169 ± 0.042	3.538ThrSer: 3.538 ± 0.049	3.523ThrThr: 3.523 ± 0.071	3.971ThrVal: 3.971 ± 0.057	0.71ThrTrp: 0.71 ± 0.026	1.698ThrTyr: 1.698 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	4.891ValAla: 4.891 ± 0.067	0.653ValCys: 0.653 ± 0.023	3.187ValAsp: 3.187 ± 0.049	4.149ValGlu: 4.149 ± 0.063	2.541ValPhe: 2.541 ± 0.046	4.209ValGly: 4.209 ± 0.07	0.969ValHis: 0.969 ± 0.024	4.929ValIle: 4.929 ± 0.067	3.659ValLys: 3.659 ± 0.055	6.107ValLeu: 6.107 ± 0.074	1.342ValMet: 1.342 ± 0.03	3.229ValAsn: 3.229 ± 0.056	2.682ValPro: 2.682 ± 0.05	2.637ValGln: 2.637 ± 0.048	2.916ValArg: 2.916 ± 0.05	4.005ValSer: 4.005 ± 0.059	3.656ValThr: 3.656 ± 0.06	4.343ValVal: 4.343 ± 0.076	0.781ValTrp: 0.781 ± 0.026	1.819ValTyr: 1.819 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	0.738TrpAla: 0.738 ± 0.024	0.159TrpCys: 0.159 ± 0.01	0.647TrpAsp: 0.647 ± 0.026	1.045TrpGlu: 1.045 ± 0.028	0.611TrpPhe: 0.611 ± 0.02	0.956TrpGly: 0.956 ± 0.025	0.346TrpHis: 0.346 ± 0.016	0.877TrpIle: 0.877 ± 0.023	0.682TrpLys: 0.682 ± 0.026	1.87TrpLeu: 1.87 ± 0.054	0.278TrpMet: 0.278 ± 0.013	0.653TrpAsn: 0.653 ± 0.025	0.278TrpPro: 0.278 ± 0.015	1.24TrpGln: 1.24 ± 0.031	0.762TrpArg: 0.762 ± 0.027	0.793TrpSer: 0.793 ± 0.027	0.615TrpThr: 0.615 ± 0.02	0.864TrpVal: 0.864 ± 0.026	0.205TrpTrp: 0.205 ± 0.013	0.415TrpTyr: 0.415 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.011TyrAla: 2.011 ± 0.042	0.388TyrCys: 0.388 ± 0.016	1.534TyrAsp: 1.534 ± 0.04	1.763TyrGlu: 1.763 ± 0.038	1.406TyrPhe: 1.406 ± 0.038	2.0TyrGly: 2.0 ± 0.046	0.759TyrHis: 0.759 ± 0.026	1.984TyrIle: 1.984 ± 0.04	1.451TyrLys: 1.451 ± 0.033	3.841TyrLeu: 3.841 ± 0.06	0.454TyrMet: 0.454 ± 0.019	1.466TyrAsn: 1.466 ± 0.036	1.694TyrPro: 1.694 ± 0.036	2.314TyrGln: 2.314 ± 0.048	1.782TyrArg: 1.782 ± 0.042	2.058TyrSer: 2.058 ± 0.04	1.631TyrThr: 1.631 ± 0.038	1.55TyrVal: 1.55 ± 0.031	0.559TyrTrp: 0.559 ± 0.022	1.17TyrTyr: 1.17 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4476 proteins (1402120 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---